Objective To explore the clinical effect and safety of bisoprolol combined with methimazole in treatment of hyperthyroidism.Methods 100 cases with hyperthyroidism were randomly divided into control group (methimazole treatment)50 case.$and treatment group(bisoprolol combined with methimazole treatment)50 cases.The therapeutic effect,serum levels of FT3,FT4 and s-TSH were observed.Results The total effective rate in treatment group 92.0% was significantly higher than 78.0%in control group(χ2=3.798,P＜0.05);The contents of TT3,TT4,FT3,FT4 after treatment were lower than before treatment(t=2.245,2.315,2.411,all P＜0.05);The contents of S-TSH was higher than before treatment(t=2.335,P＜0.05);Hyperthyroidism exponential integral in treatment group decreased significantly than the control group(t=2.312,P＜0.05).Conclusion The bisoprolol combined with methimazole treatment could efficiently improve clinical symptoms,raise therapeutic effect in patients with hyperthyroidism.

To investigate the effects of Tacrolimus ointment on patients with erosive oral lichen planus. A total of 62 cases were collected and divided into two groups randomly: 31 cases were treated with Tacrolimus ointment (treatment group), and 31 cases with clobetasol propionate ointment (control group). There was no significant difference between two groups of the effective rate and side-effect occurrence during the therapy, and relapse rate within six months after treatment. The results of the present work demonstrate that Tacrolimus ointment is an effective therapy for oral lichen planus.

Objective To examine the age-related morphological changes in hair cell nuclei,and explore the death modes of cochlea hair cells in aged rats.Methods Thirty-two Wistar rats were used in present experiment.The animals were assigned to one of the two groups,rats in aged group(n=20)were 22-23 months of age,and those in young group(n=12)were 2-3 months of age.The auditory brainstem response(ABR)thresholds of both ears elicited with tone bursts at 4,10 and 20 kHz were measured in both young and aged Wistar rats.Upon completion of the auditory test,animals were decapitated and both left and right bullae were exposed.Following fixation,whole specimens comprising the basilar membrane with Corti's organ were separated from the modiolus.Propidium iodide(PI),a popular DNA intercalating fluorescent probe,was used to trace the morphological changes in cochlea hair cell nuclei in the aged rats.Each Corti's organ was thoroughly inspected from the apical to the basal turns of cochlea with fluorescence microscopy.According to the morphological changes in the nuclei,the death modes of cochlea hair cell were determined.Results There were significant differences on ABR thresholds(P=0.001)at all tested frequencies between the young and aged rats.Three types of cochlea hair cell pathology appeared in the aged rats,including karyopyknosis,nuclear swelling and denucleation.A large number of loss or degenerated hair cells were present in the apical and basal end of cochlea in aged rats.Conclusion The present study indicates that apoptosis and necrosis are the death modes of cochlea hair cells in aged Wistar rats.

Basic medical theory integrating with clinical practice is the motivation of medical development.In our country,basic medical theory research levels has been low because of mismatch between clinic practice and basic research in lots of affiliated hospitals of medical colleges.In this paper,according to analysize the basic medical research status of several affiliated hospitals of Shantou University Medical College,we think that it is key for enhancing basic research levels to strengthen building clinical research platform,standardize management of scientific research and guide medical staffs carrying out basic theory research from clinic practice.

A dynamic model of disease can be used to quantitatively describe the pattern and characteristics of disease transmission, predict the disease status and evaluate the efficacy of control strategy.This review summarizes the basic transmission dynamic models of echinococcosis granulosus and their application.

Objective To investigate the different expression of NF-κB p65 Protein in placental tissues between premature delivery and term delivery and to explore the significance of nuclear factor kB (NF-κB) protein expression in preterm delivery. Methods Fifty premature delivery pregnant women and thirty term delivery pregnant women ( were enrolled in this study. According to the way of delivery , the patients were divided into two groups. The expression of NF-κB p65 protein was analyzed by using immunohistochemistry method in the placental tissues. Results In the term delivery, NF-κB p65 was mainly negative or weak positive expressed in the cytoplasm of cells in the placental tissues. There was no demonstrable difference in immunostaining of the NF-kB p65 subunit in the placental tissues including chorion, umbilical cord and fetal membranes before and after labor ( P ＞ 0.05 ). In the premature delivery, NF-κB p65 was strongly expressed in the cytoplasm and nuclear of cells. The expression of NF-κB p65 protein in chorion and fetal membranes from premature delivery( 18% ,56% ) was significantly higher than that from normal pregnant women (6.7% ,13.3%, P ＜0.01 ). But no significant change was found for NF-κB p65 expression in umbilical cord in pregnant women with or without premature delivery ( P ＞ 0.05 ). And in fetal membranes and deciduas, there was a significant increase in the staining of immunoreactive NF-κB p65 in preterm(52.9% ,58.8% ) by caesarean section compared to tissues obtained from term delivery( 13.3%,6.7%, P ＜0.01 ). In chorion and fetal membranes, there was significant increases in the staining of immunoreactive NF-κB p65 in preterm ( 24.2%, 57.6% ) by vaginal dehvery compared to tissues obtained term delivery (6.7%, 13.3%, P ＜0.01 ). Conclusion In this study, the expression of NF-κB p65 did not show significant change in term delivery before and after labor. NF-κB p65 in premature delivery was higher than term delivery, and it had no relationship with the delivery ways.

Objective:To investigate the value of NMO-IgG in diagnosis of neuromyelitis optica(NMO).Methods:A total of 120 patients with NMO,including high-risk NMO(HR-NMO),multiple sclerosis(MS),and other neurological diseases were enrolled in the study.The titres of serum NMO-IgG in all patients’ were detected by indirect immunofluorence.Results:The sensitivity and specificity of NMO-IgG were 67%,92% respectively in diagnosis of NMO.There were significantly differences in serum NOM-IgG posieivity among patients with NMO and MS who experienced severe optical neuritis,transverse myelitis,spinal-cord lesion beyond 3 segments as well as the patients with normal MRI scan in brain brain normal in MRI scan,serum and accompanied with other immunological disorders(P

Objective To explore the relationship between family care and postoperative cognitive dysfunction(POCD) in patients undergoing spinal surgery.Methods Patients undergoing spinal surgery were questionnaired by Family APGAR Index and Mini-Mental State Examination (MMSE) 1 week after operation.They were divided into the POCD group and non-POCD group,and the scale of Family APGAR Index was compared between the two groups.Then the pearson correlation analysis and multivariate linear regression analysis were used to explore the effect of family care on POCD.Results ①35％ (35/100) patients undergoing spinal surgery sufferd POCD.②The partnership,affection,resolve and the total score of Family APGAR Index in the POCD group ((1.25 ± 0.45),(1.36 ± 0.41),(1.27 ± 0.42),(5.85 ± 1.30)) were significantly lower than that in the nonPOCD group((1.63 ± 0.56),(1.63 ± 0.52),(1.65 ± 0.51),(6.74 ± 1.56)) (P ＜ 0.05).③Pearson correla tion analysis showed that the MMSE was negatively correlated with the partnership,affection and resolve(r=-0.426,-0.382,-0.482,P ＜ 0.05).④Multivariate linear regression analysis showed that the resolve,partnership,affection were the influence factor of POCD in patients undergoing spinal surgery.Conclusions The decline of family care can lead to POCD.

Objective To investigate the influence of retinoic acid (RA) in differentiation,maturation and function of intestinal dendritic cells (DC),and probe into the effect of Vitamin A (VA) on intestinal mucosal immunity and pathway. Method Rat intestinal mucosa was cultured in vitro,supplemented with all-trans RA and/or retinoid acid receptor ? (RAR?) antagonist (Ro 41-5253). After cultured for 24h and 48h,the surface markers OX62、OX6 and CD86 on DCs were detected by flow cytometry,to observe the effect of RA on differentiation and maturation of DC. The cytokines and RAR? were measured in mRNA levels by RT-PCR to analyze the influence of RA on mucosal immunity and the pathway. Results RA promoted the maturation of DC in mucosa cultured in vitro,and up-regulated RAR? mRNA levels,the changes were marked at 24h. Moreover,when RA was present in the culture,IL-12 and IFN-? (Th1 cytokine) mRNA were reduced,and IL-10 was increased significantly compared with control . However,the actions of RA above can be inversed by Ro 41-5253. Conclusion The modulation of RA on DC could be one of the important mechanisms that VA influences intestinal mucosal immunity. RAR? participates in the regulation of RA on DC.

Objective To examine the role of mitochondrial energy-conversion efficiency in regulating the initiation and execution of the apoptotic process in outer hair cells(OHCs)of cochlea following exposure to intense noise.Methods Seventeen adult chinchillas were used in present study.The animals were randomly assigned to one of the two groups,12 animals were exposed to noise and the remaining 5 animals without exposure to noise served as normal control.For all the animals,3-nitropropionic acid solution(3-NP,50mmol/L),an irreversible inhibitor of succinate dehydrogenase(SDH),was dropped onto the round window of the cochlea of the right ear using a micro-syringe to inhibit the mitochondrial energy production,to serve as the test ear.Artificial perilymph solution(AP)was dropped onto the round window of the cochlea of the left ear to serve as the control ear.16h after application of 3-NP and AP,the animals were exposed to 75 pairs of impulse noise at 155 dB pSPL.The cochleae were harvested 2h after the noise exposure.The cochlear basilar membrane was stained with propidium iodide(PI),a DNA intercalating fluorescent probe used to visualize the morphologic viability of hair cell nuclei.All the specimens were examined with a fluorescence microscope.Results In the 3-NP-treated cochlea,medium degree of nuclear condensation of OHCs appeared to be the dominant nuclear pathology,and only a few OHCs showed nuclear fragmentation in the damaged area of the cochlea.In contrast,the AP-treated control ear exhibited a large quantity of nuclear fragments,and a small quantity of medium degree of nuclear condensation.Conclusion The present study indicates that mitochondrial energy-conversion function plays an important role in regulating the apoptotic process.Disruption of the mitochondrial energy can not deter the apoptotic process from initiation,but can slow down the pace of apoptotic progression in outer hair cells of the cochlea following exposure to intense noise.