Objective To compare the detection rate of Xpert MTB/RIF test and sputum smear and culture method, and to investigate the value of the detection of the smear negative pulmonary tuberculosis.Methods 69 patients with tuberculosis diagnosed in Tianjin Haihe Hospital from July 2014 to July 2015 were examined by fiberoptic bronchoscopy ( and check of tubercle bacillus in sputum smear negative three times ) .With final diagnosis of pulmonary tuberculosis patients the number of statistical smear, culture method and Xpert MTB/RIF test their tuberculosis detection rate.Results A total of 69 patients with sputum smear negative patients with final diagnosis of 54 cases of pulmonary tuberculosis, confirmed 54 cases of tubercle bacillus in sputum smear negative pulmonary tuberculosis patients, Xpert MTB/RIF test was positive in 33 cases accounted for 61.11%(33/54).Among the 35 cases, 17 patients with Xpert MTB/RIF positive, can improve the detection rate of bacterial negative tuberculosis 31.48%(17/54), 19 cases of patients with negative smear positive, 16 cases of Xpert MTB/RIF positive, and the culture method was 84.21%(16/19).BALFXpert MTB/RIF test of tuberculosis found in 61.11%(33/54), the culture method of tuberculosis found 35.19%(19/54).The report is more than 50%, also has reached to 80%, and the selection has a great relationship.Xpert method and culture method, the difference was statistically significant (P<0.05).The positive rate of Xpert test was positive in 2 cases of positive MTB/RIF in the patients with positive smear positive 100%.Conclusion It is a simple, rapid and accurate method to do Xpert MTB/RIF test for BALF, and it can improve the smear negative tuberculosis patients found rate of 48.75%.The positive rate of the sputum smear negative is 59.62%(14+17/35+17), which is better than that of sputum smear and culture.

Generalized epilepsy with febrile seizures plus is a novel and common generalized epilepsy syndromes, with significant genetic and phenotypic heterogeneity. Research on genetic position and mutant of generalized epilepsy with febrile seizures plus has been a hot spot, which has importance in clarifying epilepsy syndrome as a kind of channelopathy.

Acute Disease ; Adult ; Arsenic Poisoning ; Female ; Humans ; Male ; Middle Aged ; Young Adult

Objective To Investigate the disorder of colonic epithelium apoptosis and proliferation as well as its role of the epithelial barrier break down in ulcerative colitis(UC). Methods Fifty biopsy specimens were obtained endoscopically from 35 patients of UC and 15 controls respectively.The specimens were used for diagnosis of UC and investigation of epithelium proliferation an d apoptosis by IHC with markers of ki－ 67 and M30 respectively. Results Apopto sis index(marked by M30) and ratio of apoptosis to proliferation increased obvio usly in UC compared with the controls(P0.05);Disorder of epithelium apoptosis an d proliferation take place in UC.Apoptosis cells were found not only at luminal surface but also at the base of crypt;however,proliferation cells may extend to superior part of the crypt. Conclusion Unlimited and premature apoptosis as wel l as disorder of apoptosis to proliferation is maybe one of the causes which lea ding to breakdown of the epithelial barrier function i n UC.

The absorptioa of zinc in the fermented soybean weaning food was studied using methed of neutron activation analysis of a stable isotope 70Zn. The results indicated that the rate of zinc absorption (38.7%), activity of alkaline phosphatase in serum (102.3V/L) and the increment of weight (0.5kg) of the infants (7-18m) eating the soybean fermented weaning food were higher than those eating the nonfermented soybean weaning food (30.9%, 71.0V/L, 0.4kg) and the traditional grain weaning food (rice gruel and noodle) (27.5%, 61.5V/L, 0.4kg)

Objective To explore the practicability and clinical significance of gastric tube insertion by left recumbent position for patients with exanimation. Methods We divided 100 patients with exanimation who needed gastric tube insertion into the routien group and the left-recumbent-position group with 50 cases in each group randomly and adopted routine insertion method and insertion by left recumbent position.The success rate of tube insertion and incidence of complications were observed in the two groups.Results The one-time success rate of insertion was higher while the incidence of complication was lower in the left-recumbent-position group compared with the control group (P<0.05). Conclusion Gastric tube insertion by left recumbent position proved to be a desirable method in that it could increase the onetime success rate of insertion and decrease the complications.So it worth widely applying.

Aim To observe the effects of liver cirrho-sis on the expression of transforming growth factor-β1 ( TGF-β1 ) and ColⅠin rat myocardium and interven-tion of erythropoietin ( EPO ) . Methods Thirty-six male Sprague-Dasley rats were randomly divided into three groups:control group, liver cirrhosis group and EPO group, then the cardic hemodynamic parameters in vivo and levels of serum lactate dehydrogenase ( LDH ) as well as creatine kinase isoenzyme ( CK-MB) were measured. With Masson′s trichrome stain, changes of collagen formation of myocardial tissue in different groups were observed. Also the mRNA ex-pressions of TGF-β1 and ColⅠin myocardium were de-tected by RT-PCR. Results In contrast to control group, rats in liver cirrhosis group showed a decline in systolic and diastolic function of left ventricule, rising myocardial enzyme, a distinct increase of cardiac colla-gen deposition, as well as an elevation of TGF-β1 and ColⅠmRNA expressions. In contrast to liver cirrhosis group, rats in EPO group demonstrated an improve-ment in systolic and diastolic function of left ventricule as well as in cardiac collagen deposition, and a de-crease in both myocardial enzyme and TGF-β1 and ColⅠmRNA expressions. Conclusion Liver cirrhosis can lead to the changes of myocardial structure and function in rats,and it can accelerate myocardial inter-stitial fibrosis; EPO can protect the myocardial injury in liver cirrhosis rats.

Objective To study the effect of oxygen therapy combined with recombinant epidermal growth factor on cervical cancer radioactive dermatitis. Methods From October 2013 to October 2015 in Guangxi tumor hospital, 70 stage IIIB cervical cancer patients with radioactive dermatitis from intensity modulated radiation therapy (IMRT) were enrolled in the study, 35 cases with dermatitis occurring on odd date as the study group and another 35 with dermatitis occurring on even date as the control group. The former were treated with oxygen therapy combined with epidermal growth factor and the latter with recombinant epidermal growth factor alone. The two groups were compared in terms of curative effect, wound healing time and pain. Results The total effective rate of the research group was significantly higher than that of the control group (P<0.01). The healing time of the research group was significantly shorter than that of the control group (P < 0.001). Conclusion The oxygen therapy combined with recombinant epidermal growth factor is more effective in improving the cure rate, shorten healing time of dermatitis, reduce the pains from radiotherapy and help them through the radiotherapy process.

Objective The core mechanism of renal insterstitial fibrosis (RIF) is epithelial-mesenchymal transition.This study aimed to investigate the effect of erythropoietin on high glucose-induced epithelial-mesenchymal transition ( EMT) of normal hu-man kidney proximal tubular epithelial cells (HK-2) and its possible mechanism. Mothods HK-2 cells cultured in vitro were ran-domly divided into a blank control group , a high glucose induction group , a mannitol induction group , an EPO induction group , an EPO (5, 10, and 20U/mL) inhibition group, and an Rho kinase inhibitor group.After 24 hours of intervention, the mRNA levels of RhoA and ROCK were determined by RT-PCR, those of E-cadherin and α-smooth muscle actin (α-SMA) proteins detected by immu-nofluorescence staining , and the expression of FN proteins in the supernatant measured by ELISA . Results Compared with the blank control group , the expressions of RhoA and ROCK 1 mRNA were significantly increased in the high glucose induction group (0.945 ±0.132 vs 1.400 ±0.022, 1.007 ±0.002 vs 1.913 ±0.011, P<0.05), but markedly decreased in the 5, 10, and 20U/mL EPO inhibition groups (1.400 ±0.022 vs 1.278 ±0.006, 1.400 ±0.022 vs 0.770 ±0.005, 1.400 ±0.022 vs 0.334 ±0.009, P<0.006) in comparison with the high glucose induction group , and the effects were related to the concentration of EPO .Compared with the blank control, the expression of E-cadherin protein was increased in the high glucose induction group (0.644 ±0.006 vs 0.107 ± 0.004, P<0.05), but remarkably decreased in the 5, 10, and 20 U/mL EPO inhibition groups (0.236 ±0.006, 0.433 ±0.010, 0.521 ±0.010) in comparison with the high glucose induction group (P<0.05), and the effects were also related to the concentration of EPO.Pearson correlation analysis showed a positive correlation between the mRNA expressions of RhoA and ROCK 1 in the high glu-cose induction and EPO inhibition groups . Conclusion EPO can inhibit high glucose-induced epithelial-mesenchymal transition of normal human kidney HK-2 cells and thus delay renal fibrosis , which mignt be related to the RhoA/ROCK signaling pathway .

Objective To study the role of echocardiography in case selection in transcatheter closure of perimembranous ventricular septal defect (VSD) with occluder.Methods Transcatheter closure was conducted on 26 patients with perimembranous VSD. Occluders were made of China. The images were acquired with transthoracic echocardiography. Results Twenty four patients were implanted with occluders successfully. The VSD diameter by echocardiography was 4-10 mm, average was 6.4 mm.The implanted occluder device was 2 mm larger than VSD,the maximal one was 12 mm. Perimembranous VSDs were classified by subinfracristal ( 5 cases ),membranous(6 cases), submembranous (13 cases),and the minimum distance between VSD and aortic valve annulus was 2 mm,between VSD and tricuspid valve was 3 mm. Membranous VSD was easy to be occluded. Echocardiography immediately after placement showed no residual shunt in 21 patients. The one week follow up examination showed complete closures in 21 patients. Echocardiography immediately after placement showed mild residual shunts in 3 VSD, the residual shunt disappeared after one month in one patient, two patients had mild shunt. Two VSD abandoned the occlusion because of aortic valve regurgitation increasing. Conclusions Echocardiography plays an important role in case selection.