OBJECTIVE： To prepare pefloxacin mesylate eye drops.METHODS： The content of pefloxacin was determined with UV－ spectrophotometry.The therapeutic effect of the eye drops was observed in 170 out－ patients.RESULTS： The linearity range was 2～ 14μ g/ml with a regression equation of A=0.1 018C and r=0.9 999(n=7).The average recovery was 99.64％ with a RSD of 0.43％ .After treatment,150 out－ patients recovered,16 out－ patients improved and 4 out－ patients had no efficiency with a total effective rate of 97.6％ .CONCLUSION： The eye drops has definite therapeutic effect.The UV－ spectrophotometry method is simple,rapid and accurate in determination of the content and no special reagent is needed.

Objective To observe the effect of TGF‐β1 in the endothelial‐mesenchymal transition EndM T of glomeruli ,and to explore the effect of TGF‐β/Smad signaling pathway mediated by integrin linked kinase(ILK ) in the progress of renal fibrosis . Methods Human glomerular endothelial cells (HGEnC) incubated in vitro were divided into blank control group ,TGF‐β1 (12 .5 , 25 .0 ,50 .0 ng/mL) group .TGF‐β1 50 .0 ng/mL receptor type one antagonist (LY364749)5 .0 μmol/L group ,ILK (QLT‐0267)5 .0μmol/L antagonist group .The mRNA level of P‐Smad 2/3 and ILK was determined by RT‐PCR ,and the protein level of P‐Smad 2/3 ,ILK ,E‐cadherin ,CD31 ,α‐SMA and FSP‐1 was determined by Western blot after 48 h and 72 h after incubation in each group un‐der the above‐mentioned condition .Results (1)TGF‐β1 could significantly increased the mRNA level of P‐Smad2/3 and ILK (P<0 .01) ,and the protein level of P‐Smad2/3 ,ILK ,E‐cadherin ,CD31 ,α‐SMA as well as FSP‐1 (P < 0 .01) in the concentration‐de‐pendent manner ,but significantly decreased the protein level of E‐cadherin and CD31 ( P < 0 .01) in the concentration‐dependent manner ;(2)TGF‐β1 receptor type one antagonist and ILK antagonist significantly inhibited the increasing mRNA level of ILK (P<0 .01) ,and the protein level of ILK ,E‐cadherin ,CD31 ,α‐SMA as well as FSP‐1 (P< 0 .01) induced by TGF‐β1 in the concentration‐dependent manner ,and significantly inhibited the decreasing protein level of E‐cadherin and CD31 induced by TGF‐β1 in the concen‐tration‐dependent manner(P< 0 .01) ;(3)TGF‐β1 receptor type one antagonist significantly inhibited the increasing mRNA and pro‐tein level of P‐Smad2/3(P< 0 .01) ,but ILK antagonist had no effect to the increased mRNA and protein level of P‐Smad2/3 (P>0 .05) .Conclusion TGF‐β1 as the effector molecule in downstream can promote endothelial‐mesenchymal transition of HGEnC ,and TGF‐β/Smad signaling pathway mediated integrin linked kinase participate in this process ,which probably play important role in the progress of renal fibrosis .

Objective To evaluate the diagnostic value of dynamic contrast enhanced MRI (DCE-MRI)in angiogenesis and invasion of ovarian tumors .Methods 98 patients with pathological-confirmed ovarian tumors without any treatment underwent plain MRI and DCE-MRI before operation .The plain MR images and DCE-MRI data were retrospectively analyzed .Value of Ktrans and Ve was detected by pharmacokinetic model .The expression of VEGF and MMP-2 in ovarian tumors was examined by immunohistochemistry .The correlation between DCE-MRI parameters and immunohistochemical results was analyzed .Results The expression of VEGF and MMP-2 was sig-nificantly higher in the malignant group compared with that in the benign group (both P<0.05).The value of Ktrans and Ve was significantly lower in the benign group compared with that in the malignant group ( both P<0.05) .The value of Ktrans and Ve was positively correlated with the expression of VEGF and MMP-2.Conclusion DCE-MRI can noninvasively evaluate the angiogenesis and invasion of ovarian tumors in vivo , which has important signifi-cance for clinical treatment and prognostic assessment .

Cell Differentiation ; Choristoma ; pathology ; Humans ; Soft Tissue Neoplasms ; pathology ; Thymoma ; pathology ; Thymus Neoplasms ; pathology

Objectives To study the effect of highway transportation on the quality of red blood cells. Methods Highway transportation environment was simulated by random vibration test. Red blood cell suspensions and whole blood were vibrated for 120 minutes based upon GB/T 4857.23-2003 with the RMSVA of 0.38 and 0.52grms, as well as GJB 2711-1996 with the RMSVA of 1.04grms and weight loading of 60kg. Five ml of specimen were taken before the testing (as Control Group), and after the testing at 30mins' intervals (as Testing Group). K+, FHb and red blood cell osmotic fragility were tested. Results The red blood cell osmotic fragility of whole blood increased in 0.38grms, 90min and 0.52grms, 60min specimens (P

Objective To investigate the etiologic factors of chronic prostatitis following sexually transmitted urethritis. Methods The pathogens have been detected in 86 patients with chronic prostatitis following sexually transmitted urethritis. Results 57 strains (66.3%) of bacteria were cultured in expressed prostatic secretions(EPS) from 86 patients,in which gonococcus was the majority of cultures,accounting for 38.4%. Chlamydia trachomatis (CT), ureaplasma urialyticum (UU) and gonococcus were also detected by polymerase chain reaction (PCR) in expressed prostatic secretions,25.6%(22/86), 22.0%(19/86),and 43.0%(37/86) showed positive results respectively. Conclusions Gonococcus,chlamydia trachomatis and ureaplasma urialyticum were the main pathogens of chronic prostatitis.The results indicate that pathogen examination plays an important role in treatment of chronic prostatitis following sexually transmitted urethritis.

Objective To study the clinicopathologic features and immunophenotype of cutaneous NK/T cell lymphoma and the association with Esptein－ Barr virus (EBV) infection. Methods Five cases of cutaneous NK/T cell lymphoma were studied. Histologic sections of embedded tissues were immunohistochemically stained with CD45RO, CD3? , TIA－ 1, CD20, Ki－ B5, CD68 and LMP1 antibodies, and in situ hybridization for EBV－ encoded small nuclear RNAs－ EBER1/2 was also carried out. Results Cutaneous NK/T cell lymphoma accounted for 5.68％ in the cutaneous malignant lymphoma in the same period of observation. There were 4 male and 1 female patients, and the mean age was 34 years. The main clinical manifestations were asymptomatic nodules and masses, which were accompanied by ulcers in 2 cases. Histologically, the tumors were mainly located in the dermis and subcutaneous adipose tissue, the infiltrated lymphoid cells were pleomorphic, and angiocentricity and angiodestruction were found in almost all cases. Immunohistochemical stain showed that five cases were T cell phenotype and the expression rate of TIA－ 1 was 100％ (4/4). The EBV was present in three of four cases by in situ hybridization. Conclusions Cutaneous NK/T cell lymphoma presents with unique and diagnostic clinicopathologic features, and is strongly associated with EBV.

Objective To explore the effect of serum leptin on puberty development in schoolboys.Methods 170 health schoolboys were investigated on the first spermatorrhea time and detected the levels of serum leptin, testosterone,follicle stimulating hormone (FSH), luteinizing hormone (LH). Case-controlled study was carried by multivariable logistic regression in puberty development of schoolchildren with and without spermatorrhea. Meanwhile, follow-up studies on the independent influencing factors on the puberty development were applied to this investigation in continuous two years.Results The levels of serum leptin, testosterone,FSH and LH of schoolboys with spermatorrhea were significantly higher than those of schoolboys without spermatorrhea. Multiple logistic regression analysis demonstrated that the serum leptin,testosterone, FSH, and bodyweight were the independent influence factors in puberty of the schoolboy.The levels of serum leptin,testosterone of pre-spermatorrhea schoolboys were significantly higher than schoolboys without spermatorrhea.Conclusions Serum leptin,testosterone, FSH, and bodyweight were the independent influence factors in puberty of the schoolboy.The levels of serum leptin and testosterone had obviously changed before spermatorrhea.

The application and studies of reduced diameter implants(RDI)are increasing recently.A case with fracture of three 3.3 mm ITI implants in maxilla was reported in this paper.Combined with the related literature,overload,mechanical complications(such as fatigue, crevice corrosion and hydrogen embrittlement)and the insufficiency of mechanical properties of RDI may lead to the fracture.

Objective To probe into the anatomy and clinical application of the facial nerve canal surface by multislice spiral CT image curved planar reformation. Methods Fifty patients with facial nerve disease were selected as study group from January to November in 2010, 50 healthy subjects were selected as control group, using models of multislice spiral CT scan for the patient, scanning from the upper edge of the orbital under external auditory canal edge of the connection as a horizontal line, ranging from the lower edge of mastoid process to the petrous bone on the edge of the data obtained in the Wizard workstation reconstruction. All the measurements were more than two measurements of the average physician. Results Normal facial nerve canal in control group could be displayed clearly in an image, display rate was 100.00%(50/50), and the segment data could be accurately measured; axial reconstruction, reconstruction of coronal and sagittal reconstruction of the three comparison of measured direction, fan sections, horizontal and vertical segments of the length and diameter were not significantly different (P＞0.05), and the first and second bending angle of bending angle was no significant difference (P ＞ 0.05 ). Compared with control group, the patients with vascular disease in study group showed significantly decreased rate, the difference was significant (P ＜ 0.05). Conclusion Curved planar reformation of multislice spiral CT diagnosis of facial nerve canal disease has great clinical significance, should be widely applied.