Objective To evaluate the effectiveness of Nicked-Titanium Shape-Memory alloy net-like stent in the treatment of high risk patients with benign prostatic hyperplasia Methods 35 high risk patients with benign prostatic hyperplasia were treated with Nicked-Titanium Shape-Memory alloy net-like stent from 1995 to 1998. The results were analyzed retrospectirely. Results 34 cases of the disease were treated successfully at the first session, 30 of whom could urinate immediately after treated. 4 patients had a normal urination in 3 days after operation, the total efficacy rate being 97.1% (34/35). Conclusions The present study shows that Nickel-Titanium Shape-Memory alloy net-like stent for patients with benign prostatic hyperplasia is a safe, efficient and ideal method. The long term efficacy of the stent is in progress.

Objective To report and summarize the improvement of diagnosis and treatment of male pseudohermaphroditism. Methods Among the 21 cases (mean age of 13 years,range from 6 to 31 years),19 were socially female sex and 2 were male sex.Visual clinical examination indicated abnormal external genitalia,lumps on both sides of inguina or labia majora pudendi.Genetic examination showed that the sex chromosome karyotypes of all cases were 46,XY.Before operation diagnoses were established in 20 cases by blood biochemistry,B-ultrasound and CT examination with misdiagnosis in only 1 case. Results All the 21 cases received operation.Female features were retained in 19 cases and male features in 2.Of them 15 cases were followed up for 3 to 8 years.Of the 15 cases 14 were treated by hormone replacement (oral stilbestrol)with obvious improvement of secondary sexual character.Of the 14 cases 11 had normal prepuberal physical development.The appearances of the external genitalia were satisfactory.One patient who maintained male sex had obvious growth of breasts during puberty. Conclusions Early diagnosis and treatment are very important to the therapeutic effects and the life quality of the patients.It is suggested that operation of maintaining female sex can be preferred as treatment of choice for male pseudohermaphroditism.

Objective To investigate the etiologic factors of chronic prostatitis following sexually transmitted urethritis. Methods The pathogens have been detected in 86 patients with chronic prostatitis following sexually transmitted urethritis. Results 57 strains (66.3%) of bacteria were cultured in expressed prostatic secretions(EPS) from 86 patients,in which gonococcus was the majority of cultures,accounting for 38.4%. Chlamydia trachomatis (CT), ureaplasma urialyticum (UU) and gonococcus were also detected by polymerase chain reaction (PCR) in expressed prostatic secretions,25.6%(22/86), 22.0%(19/86),and 43.0%(37/86) showed positive results respectively. Conclusions Gonococcus,chlamydia trachomatis and ureaplasma urialyticum were the main pathogens of chronic prostatitis.The results indicate that pathogen examination plays an important role in treatment of chronic prostatitis following sexually transmitted urethritis.

Objective To study Barrett's esophageal consistency with its histological findings,which were found with magnifying chromoendo6copy,and to advance the endoscopic diagnosis of Barrett's esophagus. Methods The patients with BE were diagnosed by endoscopy and histology, and their histological findings and endoscopic appearances of a total of 67 patients with BE were observed. Results Endoscopic appearances type of BE showed island (28 cases) ,tongue(7 cases) ,and circum ference(32 cases). Three types of BE under magnifying chromoendoscopy,and in magnifying endoscope group,type Ⅲ which were found with magnifying endoscope were verified intestinalisation epithelium pat ho-type, compared with type I and type Ⅱ,the discrepancy of them had statistical significance(P＜0.01). Conclusion Magnifying chromoendoscopy had a relatively better consistency in the diagnosis of Barrett's esophagus with histological findings.

ObjectiveTo evaluate the killing effects of MMC,5-Fu and TAM on human breast cancer cell lines (MCF-7 and MDA-MB-435S). MethodsThe MTT method and median-effect principle were used. ResultsKilling effects of drugs were enhanced as drug concentration increased while they were used alone or in combination. The median-effect concentration of TAM in conjunction with MMC was 1.65?10 -5 mmol/L,and that of TAM in conjunction with 5-Fu was 0.032 6 mmol/L. MMC was synergetic with 5-Fu at high concentration(CI1). The ratios of drug concentration and the sequence of administration were not related with the killing effects. ConclusionsA combination of MMC, 5-Fu and TAM has an increased killing effect on MCF-7 cell lines and questionable enhanced effect on MDA-MB-435S cell lines.

Objective To investigate the saponins from the Chinese materia medica Huluba (the seeds of Trigonella foenum-graecum L.). Methods To isolate and purify the single saponin by the column chromatography and dry column chromatography of silica gel H. The structural elucidation was carried on by spectroscopic evidence of (()~(13)C-NMR), FAB-MS, DEPT and the results of the fraction-hydrolysis of acquiring the secondary glucosides. Results The newly isolated saponin D was consisted by six molecules of sugar with diosgenin. The chemical structure of saponin D is: diosgenin-3-O-?-L-rhamnopyranosyl (1→3)-?-D-glucopyranosyl (1→4)-?-L-rhamnopyranosyl [(1→3)-?-L-rhamnopyranosyl] (1→4)-?-D-glucopyranosyl (1→4)-?-D-glucopyranoside. Conclusion Saponin D is a new one consisted of six molecules of su-(gar) with diosgenin.

PurposeTo investigate CT-guided needle aspiration biopsy in segmental bronchial lesions of central lung cancer., and to provide basis for developing therapeutic schedule.Materials and Methods Fifty-five cases of suspected central lung cancer with segmental bronchial lesions were selected. All patients underwent CT-guided needle aspiration biopsy. The puncture complications and the effect of different position and direction of the puncture needle on complications were observed.Results All 55 patients were punctured successfully in one time. Satisfactory biopsy specimens were collected in all patients. Pathological diagnosis rate was 90.91% (50/55). Operative pathology or post-puncture follow-up confirmed the central lung cancer with segmental bronchial lesions in 40 cases with positive rate of 72.73% (40/55), and the negative rate was 18.18% (10/55). As for puncture complications, the needle tract bleeding was seen in 19 cases (34.55%), and some pneumothorax in 4 cases (7.27%). There was statistic difference between the two complications (χ2=6.03,P<0.01). The complications of prone and supine position had no statistical difference (P>0.05). Incidence of complications decreased with performance angle by the order of vertical puncture, horizontal puncture, and 45 degrees puncture. There was statistic difference (χ2=3.68,P<0.05).Conclusion CT-guided percutaneous needle aspiration biopsy is accurate in diagnosis of central lung cancer with segmental bronchial lesions. It has no serious complication and is worthy of general clinical application.

Objective:To study the isolation, culture and identification of the TMJ cells and to observe the biological characteristics of cultured fibrochondrocytes. Methods:The TMJ discs were dissected from two 1 month goats under sterile conditions and were digested with collagenase. The cells were collected. Morphological changes and attachment efficiency were constantly observed under phase-contrast microscope. Immunohistochemical staining for type I collagen as well as toluidine blue staining were performed. Ultrastructures of the TMJ cells were observed under transmission electron microscope. Results: Most of the primary fibrochondrocytes presented a short spindle-shape while the rest showed polygon-shape. On the 7th day, the perliferating fibrochondrocytes started to contact each other to form a monolayer covering the bottom of the incubation disc. Immunohistochemical staining of type I and toluidine blue staining exhibited positive results. The fibrochondrocytes cytoplasms were rich in mictochondria and endoplasm reticulum. Conclusion: The fibrochondrcytes isolated from one-month-old goat TMJ disc have good proliferation ability in vitro and cells from passage 1 to 3 might be used as seed cells for TMJ disc tissue engineering.

Aim To investigate the effects of iptakalim(IPT),a novel K_(ATP) opener,on the functions of endothelin system in human pulmonary artery endothelial cells.Methods Primary cultured human pulmonary artery endothelial cells were incubated with different concentrations iptakalim for 24 h.The levels of ET-1 in medium were observed by radioimmunoassay.Reverse transcription polymerase chain reaction(RT-PCR)was performed to analyze the expression of ET-1 and ECE.Results When endothelial cells were incubated with IPT at concentrations above 10 μmol·L~(-1),the levels of ET-1 release in medium and the levels of ET-1 mRNA were significantly inhibited.When endothelial cells were incubated with IPT at concentrations above 1 μmol·L~(-1),the levels of ECE mRNA were significantly inhibited.Conclusions IPT can inhibit the expression of ET-1 and ECE mRNA from human pulmonary artery endothelial cells, thus it inhibits the secretion of ET-1 from endothelial cells. Iptakalim may serve as a promising candidate drug to treat pulmonary hypertension.

BACKGROUND:There is no common cognition in the cell type in the temporomandibular joint(TMJ)discs,and names describing TMJ disc cells also vary a lot.OBJECTIVE:To characterize the type and the distribution of cells in the TMJ disc of goats DESIGN,TIME AND SETTING:A single sample observation was completed in Cettutar and Motecutar Biologicat Center and Electron Microscope Center of Lanzhou University from March to May in 2007.MATERlALS:TMJ discs were obtained from two one-month-old healthy goats that were slaughtered freshly.METHODS:Bilateral TMJ discs of goats were cut off completely and were divided into 6 parts by 3 cuts in the major axis direction (mediolaterally)and 2 cuts in the minor axis direction(anteroposteriorly).Then the marked samples were fixed in 10%neutral formalin Iiquid for 24 hours and embedded by paraffin.MAIN OUTCOME MEASURES:Hematoxylin and eosin staining were used to identify regional variation of cell type and cellnumbers.Toluidine blue staining and collagen type Ⅰimmunohistochemical assay were performed to test the distribution of collagens.Transmission etectren microscopy was used to observe the ultrastructure of cells of goat TMJ discs.RESULTS:TMJ discs were comprised of cells and collagen fibers distributing unevenly.Collagens were mostly type Ⅰ.Collagen fibers were wave or crimping and approximately parallel to each other.with cells scattered in their matrix.Fibroblast-like cells and chondrocyte-like cells were the main two types of cells existing,with the former predominating over the later in a ratio of 2.05:1 approximately.There were no significant regional differences in cell type and distribution statistically.Transmission electron microscopy denoted that fibroblast-iike cells have fairly larger fusiform or irregular nuclei with very few organelles,while the chondrocyte-like cells exhibited round or elliptical nuclei,well defined pericellular electron lucent zones,unconspicuous cytocysta and non-distinctive pseudopodia CONCLUSION:There are no significant differences in type,number and arrangement of cells in TMJ discs of one-month-old goats statistically,with Fibroblast-like cells predominating slightly over chondrocyte-like cells.