ObjectiveTo investigate the clinical effect of carvedilol in the treatment of chronic heart failure patients with arrhythmia.Methods120 cases of hospitalized cases of cardiology were randomly divided into treatment and control groups by checking the random number table.The control group was given benazepril hydrochloride combined digoxin cardiac medicine and furosemide or hydrochlorothiazide,spironolactone and other diuretics in the treatment.The treatment group was given carvedilol based on the treatment of the control group.Followed up for 12 weeks,the clinical manifestations,blood pressure and heart rate,left ventricular ejection fraction(EF) and arrhythmias before and after treatment were obsered,and to determine the efficacy.ResultsThe treatment group decreased heart rate,corrected ventricular premature contraction,ventricular contraction and ventricular tachycardia in comparison with the control group with significant differences(t =6.018、5.319、7.623、6.194、7.562,all P ＜0.001 ),and there was a significantly statistical significance( P ＜ 0.01 ) that the treatment group can increase the ejection fraction compared with the control group.The markedly and total efficiency was more effective in the treatment group than the control group,which was statistically significant(x2 =34.301,P ＜0.05).ConclusionThere was a positive effect of carvedilol in improving heart function,reduceing arrhythmias and mortality,and reducing hospitalization rate and other aspects.

The emergence of drug resistance mutations in human immunodeficiency virus (HIV) genome is the major reason for antiviral treatments failure.To investigate the drug-resistance viral quasispecies in HIV-infected individuals and to find out the new drug resistance mutations have significant effects on directing effective clinical treatment and developing novel antiviral drugs.HIV drug resistance tests mainly include genotypic drug susceptibility assay and phenotypic drug susceptibility assay.We reviewed in this article some novel development in the field.

The process of apoptosis runs through a variety of signal transduction, and is regulated by many apoptosis-related genes. Inhibitors of apoptosis protein (IAPs) are closely related to the infinite proliferation of tumor cells. IAPs including the recently discovered survivin, livin, are closely related to tumors. The anti-apoptotic mechanism is to be further studied.

ObjectiveTo study the value of serum soluble intercellular adhesion molecule-1 (sICAM-1),B-type natriuretic peptide (BNP) and C-reactive protein (CRP) levels detection on assessment of illness in patients with congestive heart failure.MethodsSixty-three patients with congestive heart failure were involved in this study.The levels of serum sICAM-1,BNP and CRP were compared in patients with different NYHA cardiac function classification before and after treatment.ResultsThe levels of serum sICAM-1,BNP and CRP in NYHA cardiac function classification ≥ Ⅲ grade patients were significantly higher than those in NYHA cardiac function classification ＜ Ⅲ grade patients[ (392.6 ± 73.3) μ g/L vs.(212.6 ± 83.4) μ g/L,( 1992.5 ±1173.7) ng/L vs.(512.6 ± 283.2) ng/L,(49.3 ± 33.5 ) mg/L vs.(6.1 ± 3.6) mg/L] (P ＜ 0.01 ).The levels of serum sICAM-1,BNP and CRP after treatment were significantly decreased compared with those before treatment [ ( 168.9 ± 52.5) μ g/L vs.(362.8 ± 123.1 ) μ g/L,(68.6 ± 38.4) ng/L vs.(1470.9± 1460.1) ng/L,(3.6±2.8) mg/L vs.(34.1 ±29.8) mg/L] (P ＜ 0.01).Conclusion Serum sICAM-1,BNP and CRP levels are positively related with illness,and serum sICAM-1,BNP and CRP levels may be judged as an index assessing treatment effect and prognosis.

BACKGROUND:Fatigue measurement is conducted while the eyes are gazing at something to accomplish the determination and monitoring of a real-time and non-contact fatigue.OBJECTIVE:To figure out the percentage of eyelid closure (PERCLOS)value for fatigue and deduct the subject's fatigue state.DESIGN:To detect the subject's pupils with a reconstructed eye tracking system ,the video frequency of the pupils is collected, from which the PERCLOS value is worked out by image processing. The PERCLOS value is used to reflect the subject's fatigue state.SETTING:School of Mechanical and Electronic Engineering, Beijing Jiaotong University.MATERIALS :The experiment was conducted in the Ergonomics Lab from September 2003 to November 2004. The equipments used were video transmission cable, frame grab card and computer.METHODS:The video frequency images of pupil module sent from eye tracking system were processed to deduct the closure state of the pupil and figure out the PERCLOS value for the determination of fatigue.MAIN OUTCOME MEASURES :The images collected by image collecting card reflected the state of opening and closure of the pupil.RESULTS:A fatigue determination system which was able to determine effectively the examinee's fatigue state was established.CONCLUSION:This new method expands the application of eye tracking system which can be safely used off-line or on-line.

OBJECTIVE: To investigate the efficacy outcomes of laser assisted outpatient septal spur surgery for contact point pain in a carefully selected group of patients. METHOD: This investigation included 32 patients who were selected for laser-assisted septal spur surgery in an outpatient surgical suite. All had endoscopically visible spur contact as well as nansal CT scans. Patients of sinusitis were excluded. The area of the contact point was treated with decongestant and lidocaine. If the headache completely disappeared or diminished by more than 50% in intensity, subjects were considered candidates for surgery. Headache characteristics were assessed preoperatively and at follow-up (30 months after surgery) using a standardized questionare. RESULT: Nine cases (28.1%) were free from pain at the last follow-up; 19 cases (59.4%) had their headache scores improved after surgery; four cases (12.5%) had a less than 25% reduction in their headache score. No septal perforation, hematoma, was reported. CONCLUSION For selected patients with contact point headaches, septoplasty may be useful. The positive dicaine test may indicate better effect of operation. Laser-assisted outpatient septal spur surgery for contact point pain shows good results in short-term effect, and has less complications, but long-term follow-up is required to assess its real effect.
Adult ; Anesthesia, Local ; Female ; Headache ; etiology ; surgery ; Humans ; Laser Therapy ; Male ; Middle Aged ; Nasal Cavity ; Nasal Mucosa ; surgery ; Retrospective Studies ; Treatment Outcome ; Young Adult

Paeonol is an nerb which has been used us an anodyne. The analgesic action of Paeonol has been shown in this paper by using the following methods: Mice licking hind paw on hot plate (55℃); mice wring evoked by ip 0. 7% acetic acid 10 ml ? kg-1; continuous pain stimuli elicited by sc 2. 5% formaldehyde 0. 03 ml in fore paw of mice.No tolerance of analgesic effect was found after daily sc of Paeonol 100 mg ? kg-1for 7 d in mice assayed with hot plate method. In naloxone-challenge test, no jumping response was observed in mice treated with Paeonol 690mg ? kg-1, the maximal tolerance dose. The results indicate that Paeonol induced no morphine-like tolerance norphysical dependence.The analgesic action of Paeonol was not an-tagonizd by naloxone, but was eliminated by in-traperitoneal injection reserpine 3 mg ? kg-1 3 h prior to Paeonol. These results demonstrate that Paeonol belongs to the non-narcotic analgesic, and suggested that the analgesic action of Paeonol may be related to the monoamine level in brain.

Objective To study the changes of the rat's hearing threshold by long-term treatment with Vitamin-C and the reason of the changes. Methods Forty-two Wistar rats were randomly divided into two groups: the treatment group was treated with water added Vit-C and the placebo group was treated with drinking water.(1)Concentrations of malondialolehyde(MDA)of the cochlea with fluorescence chromatometry.(2) Auditory sensibilities with auditory brainstem response testing.(3) The frequency of the mtDNA~(4834)bp deletion in the cochlea with polymerase chain reaction technique. Results Hearing threshold of both groups become worse with aging,and the treatment group showed the better auditory sensitivities,the less concentrations of MDA and the less frequency of mtDNA~(4834)bp deletion compare with the placebo group. Conclusions Long-term treatment with Vitamin-C could reduce the degree of hearing loss of the rats and the concentrations of MDA of the cochlea and the frequency of mtDNA~(4834)bp deletion in the cochlea.The oxygen radicals theory should be the etiology of presbyacusis.

Objective To establish a quality standard for Xiaoer Shengxue granules. Method SHU-Dihuang, yam, Chinese data in Xiaoer Shengxue granules were identified by TLC. Ferrous sulfate in the granule was determined by UV with wavelength at 509 nm. Results SHU-Dihuang, yam, Chinese data could be detected by TLC. Ferrous sulfate showed a good linear relationship at the range of 0.50～2.50 mg/mL, r=0.999 9. The average recovery was 101.3% and RSD was 1.48% (n=5). Conclusion The method is available with a good reproducibility and can be used for the quality control of Xiaoer Shengxue granule.

To investigate the expression of mRNA and protein of telomerase reverse transcriptase(TERT) in guinea pig asthmatic lung tissues and human circulating lymphocytes and to discuss the role of TERT in the pathogenesis of asthma and the clinical significance of expression of TERT in human asthmatic circulating lymphocytes in monitoring the state of asthma.Methods: In 6 guinea pig asthmatic lung tissues, 6 guinea pig normal lung tissues, 16 samples of circulating lymphocytes of asthmatic patients and 14 normal circulating lymphocytes the expression of mRNA and protein of TERT were detected with nuclei acid in situ hybridization and immunostaining. The expression of TERT in circulating lymphocyte were compared with pulmonary function and serum IgE level. Results:The expression of mRNA and protein were up-regulated in asthmatic airway epithelium and lymph nodule germinal center in guinea pig. Compared with control group,high expression were also showed in human asthmatic circulating lymphocytes.The index of TERT expressive positive lymphocytes showed negative correlation with FEV1/ predictive value and MW/predicn've value,positive correlation with serum IgE level. Conclusion: (1 )TERT expression in asthmatic airway epithelium and lymph nodule germinal centers of guinea pig and human circulating lymphocytes were up-regulated; (2)Detection of TERT expression of asthmatic circulating lymphocytes may be useful to monitor the state of asthma.