BACKGROUND: Parental rearing styles has profound and long-range effects on the mental development of their children.OBJECTIVE: To investigate the relationship between parental rearing styles and its correlative factors and the mental health of medical students.DESIGN: A transactional survey study based on medical students.SETTING: Department of psychology in a clinical medical college of a normal school.PARTICIPANTS: The study was conducted in the faculty of medicine of Hangzhou Normal School in December 2002. Totally 165 students of grade 2000-2002 including 106 college students, 59 undergraduates, and 98males, 67 females were selected as subjects. The youngest was 19 years old and the oldest was 24 years old. Totally 161 students had both parents (97.60%), 4 students had single parent (2.40%), 53 students were the only child of their family (32. 10%), and 112 students were not the only child of the family (67.90%).METHODS: Egna minnen barndoms uppfostran(EMBU) and symptom checklist 90(SCL-90) was introduced to evaluate the parental rearing styles and the mental health level in 165 medical students. SPSS 10.0 software was used for correlation analysis and multifactor stepwise regression analysis.MAIN OUTCOME MEASURES: Scores for 11 factors in EMBU including father' s emotional warmth and understanding, father' s punishment and stringency, father' s excessive interference, father' s favoritism, father' s rejection of deny, father' s excessive protection, mother' s emotional warmth and understanding, mother' s excessive interference, mother' s excessive protection, mother' s punishment and stringency, and mother' s favoritism, and the selection status of the factors correlated with the parental rearing styles including the relationship between father and mother, family income, and family type, etc. Total mean and scores for 9 factors in SCL-90 including somatization, obsessive-compulsive, interpersonal sensitivity, depression, anxiety, hostility, phobic anxiety, paraniod ideation and psychoticism.RESULTS: Correlation analysis displayed that there was significant positive correlation between father' s punishment and stringency and somatization,sensitive human relationship(r=0. 186, r=0. 162), between mother' s punishment and stringency and sensitive human relationship( r =0. 185),between father' s favoritism and obsessive-compulsive, interpersonal sensitivity, depression, anxiety, hostility, phobic anxiety, paraniod ideation and psychoticism factor and total mean( r = 0. 161, 0. 243, 0. 224, 0. 182,0. 166, 0. 193), between mother' s favoritism and depression( r=0.132),between father' s excessive protection and depression, anxiety, and total mean( r = 0. 177, 0. 204, 0. 162), and between mother's rejection of deny and sensitive human relationship( r = 0. 162) . There was significant negative correlation between father' s emotional warmth and anxiety, hostile ( r =-0. 187,-0. 241 ). As indicated by multifactor stepwise regression analysis for the impacts of parental rearing styles and its correlative factors on SCL-90 total mean, rearing styles including mother' s education background and father' s excessive protection entered into regression equation (β= -0. 269, P =0. 001; β=-0. 187, P=0. 016).CONCLUSION: Parents' punishment and stringency, parents' favoritism, father' s excessive protection and mother' s rejection of deny are not good for the mental health of medical students. Father' s emotional warmth is good for the improvement of mental health in medical students. The impacts of father' s excessive protection and mother' s education background are even more important to the mental health of medical students. Hence, the improvement of mental health in students should extend to the instruction of parental rearing styles.

Objective: To explore the relationship between parental rearing styles and medical students' personality characteristics. Methods: 260 medical students were assessed by the Egna Minnen Barndoms Uppfostran (EMBU)Scale and the Eysenck Personality Questionnaire(EPQ).Results:(1) The single children obtained higher scores than non-single children on father's over-interference and parents' favorite factor of EMBU ( P

As a carrier of anti -cancer drugs ,peptides can modify and coat drugs .It shows good pros-pects in targeted drug delivery ,drug sustained -releasing,drug enhancement of solubility ,bioavailability.It can also reduce side effects of drug .The related studies become one of the hot points in the field of pharmaceutical re-search in recent years .In this paper , the types of peptide based drug delivery emerged in recent years are re-viewed,the characteristics and advantages of various kinds of carrier systems are described in details .The present review provides a theoretical basis for the further application of peptide drug carries .

Objective To screen and identify the human phage engineered antibodies against HBsAg, and to analyze their gene sequence. Methods The phage antibody library was constructed by phage surface display techniques, and then the antibody genes were isolated and amplified from human peripheral blood lymphocyte. The human phage engineered antibody against HBsAg was selected through bio-panning from the phage antibody library. The affinity and specificity of the phage antibody was identified by ELISA and inhibition ELISA assay. The genes of heavy chain and light chain of the phage antibody against HBsAg were analyzed by sequencing. Results 30 colonies were obtained after three rounds of bio-panning. One of them had the highest A490 (A490=1.47?0.08) with ELISA, and the inhibition rate was 76% with inhibition assay (A490=0.35?0.10), implying that the phage antibody against HBsAg was of high specificity. The findings of enzyme digestion demonstrated that the phage antibody included genes of heavy chain and light chain. Sequencing results indicated that the VH region of heavy chain belonged to VHI subgroup and the VL region of light chain belonged to V? Ⅰ and V? Ⅲ subgroup. Conclusions The specific human phage engineered antibody against HBsAg was selected successfully from the phage antibody library. These results suggest that screening and identification of human phage engineered antibody against HBsAg through the phage antibody library are technologically feasible. It lays a foundation for application of human engineered antibody against HBsAg to design new targeted therapy strategy for HBV.

Drug-receptor interaction plays an important role in a series of biological effects, such as cell pro-liferation, immune response, tumor metastasis, and drug delivery. Therefore, the research on drug-re-ceptor interaction is growing rapidly. The equilibrium dissociation constant (KD) is the basic parameter to evaluate the binding property of the drug-receptor. Thus, a variety of analytical methods have been established to determine the KD values, including radioligand binding assay, surface plasmon resonance method, fluorescence energy resonance transfer method, affinity chromatography, and isothermal ti-tration calorimetry. With the invention and innovation of new technology and analysis method, there is a deep exploration and comprehension about drug-receptor interaction. This review discusses the differ-ent methods of determining the KD values, and analyzes the applicability and the characteristic of each analytical method. Conclusively, the aim is to provide the guidance for researchers to utilize the most appropriate analytical tool to determine the KD values.

Objective To screen permethrin human single-chain variable region (scFv) antibody for aims of developing rapid detection kit. Methods Phage display technology was used in this study. Permethrin was solid phase coated on Nunc plate as antigen. Semi-synthetic single-chain variable region of human antibody library technology was applied, and single chain variable region was screened from phage antibody library after 3 rounds "adsorption - elution - amplification" of the selection process. 100 clones were random selected as resistance to permethrin clones , enzyme-linked immunosorbent assay (ELISA), crossreactivity and competitive inhibition experiments were used to validate permethrin binding activity with strong scFv clones from the selected phage antibody clones plasmid. The plasmid was digested with restriction enzyme Sfi Ⅰ / Not Ⅰ and subcloned into pCANTAB5E vector. After transformed into E. coli XL1BIue, the plasmid was identified by restriction enzyme analysis. Results After screening in 100 clones, 18 clones had high ELISA absorbance values ( A value) at 490nm wavelength ( A490nm), then bovine serum albumin (BSA) cross-reactions identified five weak cross-reaction. Combined with the triplicate ELISA and competitive inhibition experiment results, one positive clone was acquired at last. And this clone was subcloned into pCANTAB5E vector and transformed into competent cells XL1-Blue. Conclusion Plasmid fragment was consistent with the purpose, which provided the foundation for further study of its specific affinity.

Objective To do screening acute lymphoblastic leukemia patients scFv antibody single chain variable region to cre-ate conditions for the expression and obtain further specificity of antibody fragments.Methods In this study,patients with newly diagnosed acute lymphoblastic leukemia serum as coating antigen using phage display technology,screening phage an-tibody specificity from the semi-synthetic human phage antibody libraries,the first to target the immune antigen-coated tab-let,phage library was added,so that with the target antigen-specific binding phage antibody was immobilized on plates immu-nization,could not be specifically bound phages were rinsed.The eluted specific binding phage,E.coli infection.Could get the specific antibody gene containing phagemid.Results After three “adsorption-elution-amplification”screening process,got stronger leukemia patient antigen-specific phage antibody variable region fragment and identification.Conclusion Got better strain affinity antibody fragments,to create the conditions for the next fragment expression,identification and clinical appli-cation.

Objective To study the effect of auricular point sticking on the cerebrovascular reactivity in hypertension patients of different stages by using Transcranial Doppler (TCD) technology.Method Three hundred hypertension patients were randomized into a treatment group (auricular point sticking) of 180 cases and a control group (medication) of 120 cases. The cerebral arterial hemodynamic indexes were observed and analyzed by TCD before and after intervention.Result In comparing the improvements of cerebral arterial hemodynamic indexes in stage 1-2 hypertension patients, the treatment group was significantly superior to the control group (P＜0.05,P＜0.01); in stage 3 hypertension patients, the improvements of cerebrovascular hemodynamics of the treatment group were significantly better than those in the control group (P＜0.05,P＜0.01).Conclusion Auricular point sticking can improve the cerebrovascular hemodynamics and effectively improve the cerebrovascular reactivity of hypertension patients. TCD can help observe and estimate the cerebral arterial function of hypertension patients, and prevent and intervene in cerebrovascular diseases.

Objective To investigate the changes in plasma very long chain fatty acids (VLCFAs),and to explore its relationship with obesity-related index in obese children.Methods One hundred and sixty-six obese children aged 7 to 12 years old were investigated and 148 health children matched with age and sex were selected as control group.Their height,weight,and waist circumference (WC) were measured.The percentage of body fat (PBF) was tested by dual-energy X-ray absorptiometry.Fasting blood triglycerides (TG),total cholesterol (TC),high density lipoprotein-cholesterol (HDL-C),low density lipoprotein-cholesterol (LDL-C) levels,fasting plasma glucose,and fasting insulin (FINS) were determined.The body mass index (BMI),BMI-Z score,waist to height ratio (WHtR),and homeostasis model assessment for insulin resistance (HOMA-IR) were calculated.The plasma VLCFAs were analyzed by gas chromatography-mass spectrometry.Results (1) Compared with normal control children,body weight,BMI,BMI-Z score,WC,WHtR,PBF,TG,TC,LDL-C,FINS,and HOMA-IR in obese children were increased (all P ＜ 0.05),and the level of HDL-C was decreased (P ＜ 0.05).(2) The plasma levels of docosamonoenoic acid (C22 ∶ 1 n-9) and arachidonic acid (C20 ∶ 4n-6) were decreased while the levels of arachicacid (C20 ∶ 0) and eicosapentaenoic acid (C20 ∶ 5n-3) increased in obese children compared to non-obese children.(3) The linear correlation analysis showed that PBF was positively correlated with C20 ∶ 0 and C20 ∶ 5n-3,and negatively correlated with C20 ∶ 4n-6.WHtR was negatively correlated with C22 ∶ 1 n-9 and positively correlated with C20 ∶ 0 (4) The multiple stepwise regression analysis showed that C20 ∶ 0 was the main factor for PBF,and C22 ∶ 1 n-9 and C20 ∶ 0 were the main factors for WHtR.Conclusion The metabolic abnormality of plasma very long chain fatty acids exists in obese children.The changes in C20 ∶ 0,C22 ∶ 1 n-9,and C20 ∶ 5n-3 may be associated with obesity in children.

Objective:To evaluate the fit of titanium alloy removable partial denture framework fabricated by selective laser melting(SLM) technique.Methods:7 Kennedy Ⅲ Ti-6Al-4V removable titanium alloy partial denture frameworks were fabricated by SLM technology.An optical scanner was used to scan the gypsum model adhered with the silicone rubber film obtained by the impression method before and after removal of the film.Geomagic Qualify 2013 software was used to analyze the gap between the model and the tissue surface of the major connector of the framework,the fit of the frame work was evaluated.Results:The overall 3D deviation between the titanium alloy frameworks and the gypsum model was (0.221 9±0.07) mm.Conclusion:The fit of the titanium alloy removable partial denture framework made by SLM technology can basically met the clinical requirements.