Faced with the current intense employment competition,medical graduates have a variety of negative employment states of mentality. This article,by doing investigation,obtains the negative employment states of mentality and its main behaviors among medical graduates,analyzes the causes of this phenomenon,and then tries to find out the methods and channels to help medical graduates to relieve negative employment states of mentality. This kind of probe is helpful for medical graduates to realize full employment and smooth employment,and is helpful for medical education to keep a healthy and steady development as well.

Objective To investigate the mechanisms of early sensorineural hearing loss of diabetic rats. Methods The diabetic model for rats was established. Every rat was checked with ABR and DPOAE before and af-ter treatment . After 14 days , the blood glucose, blood fat, SOD, GSH-Px and MDA were measured. Results For the model group, the rats" blood glucose and fat increased while the GSH-Px was reduced more than the con-trol group. The response amplitudes of DPOAE decreased at 4 000 Hz and 8 000 Hz(P<0. 05). Conclusion The damage to the hair cells caused by the affected metabolism is considered as the culprit of the sensorineural hearing loss and the changes of DPOAE took place earlier than that of ABR, thus becoming useful in detecting early hearing loss of the diabetic patients.

Objectives: The aim of this study was to define whether growth hormone therapy would reduce the course of postoperative fatigue (POF) in aged patients (60～80 years old) with nutritional support. Methods: Forty aged patients who underwent partial gastrectomy or other operations were randomized into two groups. All patients were treated with the same nutritional support formula.A group of patients was treated with rhGH for 7 day from the third day after operation, and control group treated with placebo.The score of POF, nitrogen balance, transferrin level and body weight were monitored. Results: Score of POF in rhGH group on day 7 and 14 after operation was lower than that in control group(P0.05). Conclusions: Aged patients with rhGH after major operation can recover more rapidly from POF.

Objective]Not only to promote the rational allocation of resources between government, society and universities,but also to provide theoretical basis and practical path for connotational development of newly-built undergraduate college.[Methods]Focusing on“ the development situation and the demand for the connotational development of the newly-built undergraduate college”and“ the aid possibility”,five representatives of newly-built undergraduate colleges and seven aid-donors were interviewed and inquired by Likert scale .[Results]The average index of newly-built undergraduate colleges on“ the development situation” and “ the demand for the connotational development” are respectively 2.93 and 4.03,showing a good situation and strong demand for external assistance.Meanwhile, the aid-donors also show a high will on the aid possibility” ,the average index is 4.06.[Conclusion]Based on analysis of“ the development situation and the demand for the connotational development of the newly-built undergraduate college, andthe aid possibility”,our research puts forward aid model and mechanism in connotational development of newly-built undergraduate college in order to make full use of external assistance, including government,society and universities.

Objective: To investigate the relationship of murine fibrinogen-like protein 2(mfgl2) / fibroleukin with pulmonary impairment in the murine model of severe acute respiratory syndrome(SARS).Methods: The Balb/cJ mice infected with murine hepatitis virus strain 3(MHV-3) through the trachea were observed for the pathological features and virus distribution in different organs.The expressions of both mfgl2 and fibrino in the lungs were determined by in situ hybridization and immunohistochemistry.Results: The MHV-3 infected mice developed typical interstitial pneumonia with extensive hyaline membrane formation in the alveoli,presented micro-vascular thrombosis in the pulmonary vessels and died within 5 days.MHV-3 virus replication was identified in all the organs observed.The specific expression of mfgl2 prothrombinase was noted in the terminal and respiratory bronchioles,alveolar epithelia and infiltrating cells.Conclusion: The characteristics of the pulmonary impairment of SARS in human can be properly simulated by the MHV-3 induced murine model of SARS.The up-regulation of the specific gene mfgl2 in the lungs involved in fibrino deposition and microvascular thrombosis may be largely responsible for SARS-associated pulmonary damages.

Objective To study the alterations of serum amino acid level in patients with short bowel syndrome (SBS). Methods Blood levels of 17 amino acids were measured in 17 SBS patients on admission to the hospital, and 15 healthy volunteers. Results Compared with the control group, serum concentrations of Val, Leu, Ile, Lys, Met, Ser, His, Cys in patients with SBS significantly decreased ( P

Objective To construct the eukaryotic expression plasmids of human Fas and TNFR1 gene(pcDNA3.0-hFas and pcDNA3.0-hTNFR1)and microRNA(miRNA)expression plasmid of hFas and hTNFR1 named p-hFasmiRNA and p-hTNFR1miRNA,and to investigate their inhibitory effects in vitro.Methods The eukaryotic expression plasmids of human Fas and TNFR1 gene were constructed(pcDNA3.0-hFas and pcDNA3.0-hTNFR1)and have been shown successfully to express hFas and hTNFR1 protein.miRNA expression plasmids of hFas and hTNFR1 named p-hFasmiRNA and p-hTNFR1miRNA complimentary to the sequence responsible for hFas and hTNFR1 respective were constructed,meanwhile irrelevant miRNA plasmid was used as a control.By respective co-transfection of p-hFasmiRNA and pcDNA3.0-hFas,p-hTNFR1 miRNA and pcDNA3.0-hTNFR1 expression construct into 293T cells,the inhibition of hFas and hTNFR1 expression was analyzed by real-time PCR and Western blot.Results The experiments showed the significant inhibitory effect of p-hFasmiRNA on hFas and p-hTNFR1miRNA on hTNFR1 expression at 48 h post-transfection both at RNA level and protein level as well in 293T cell lines with the inhibitory efficiency being as high as 87% for hFas and 80% for hTNFR1,respectively.Conclusion The p-hFasmiRNA and p-hTNFR1miRNA were constructed successfully,and it was verified that they could specifically inhibit the hFas and hTNFR1 expression at the cellular level.

Objective To evaluate the efficacy of intensity-madulated radiotherapy(IMRT) as the primary treatment in nasopharyngeal carcinoma (NPC). Methods Between November 2001 and March 2006,147 newly diagnosed NPC patients were treated with IMRT. The disease was Stage I in 3 patients, Stage Ⅱ in 35,Stage Ⅲ in 67 and Stage Ⅳ in 42. For T1-2 disease,the prescription dose was 70 Gy to the gross tumor volume( GTVnx) and positive neck nodes ,60 Gy to the planning target volume I, and 50-56 Gy to the clinically negative neck. For T3-4 disease,the prescription dose was 74-78 Gy to GTVnx. The local control rate(LC),overall survival (OS), disease-free survival (DFS) and distant metastasis-free survival (DMFS) were estimated by Kaplan-Meier method. Results The 3-year LC, OS, DFS and DMFS of the whole group were 93.2% ,93.5% ,72.6% and 74.4% ,respectively. T stage was a significant predictor of LC and OS. For patients with T1-2 and T3-4 disease,the LC was 100% and 86.9% (P=0.007) and OS was 95.5% and 91.3% (P=0.030), respectively. N stage was a significant predictor of OS, DFS and DMFS(P=0.03,0.004 and 0.0004,respectively). The grade Ⅰ+ Ⅱ and Ⅲ acute toxieities of parotid were 96.6% and 1.4%. The rate of grade Ⅱ xerostomia at 3-month, 6-month, 1-year and 2-year after radiotherapy were 43.0%, 12.0% ,4.9% and 3.2%. Conclusions The treatment results are promising according to our target definition and dose prescription protocol for nasopharyngeal carcinoma.

Objective To understand the epidemic situation and epidemiological characteristics of malaria in Jiangsu Prov?ince from 2005 to 2014,so as to provide the evidence for formulating and adjusting the strategies and measures on malaria elimi?nation in this province. Method The epidemiological data of malaria in Jiangsu province from 2005 to 2014 were collected, and the prevalence situation,infection sources as well as the temporal,regional and population distribution of the cases were an?alyzed. Result A total of 5 069 malaria cases were reported in Jiangsu Province form 2005 to 2014,there were 3 422 cases (67.51%)of vivax malaria,1 497 cases(29.53%)of falciparum malaria,and 150 cases(2.96%)that were unclassified. Among all the reported cases,2 139(42.20%)were local cases,1 131 were(22.31%)imported cases from other places in Chi?na ,and 1 799(35.49%)were imported cases from abroad. There were 3 809 male cases and 1 260 female cases,with a sex ratio of 3.02∶1. The patients were mainly aged from 25 to<60 years(accounting for 67.15%),and were mainly young adult farmers (40.26%)and migrant workers(23.63%),who mainly distributed(accounting for 60.58%)in Xuzhou,Suqian,Huai’an,Su?zhou and Nantong cities. The predominant malaria type from 2005 to 2009 were vivax malaria,while after 2010,it changed to falciparum malaria. Conclusions The malaria epidemic situation has been effectively controlled in Jiangsu Province,which re?flects the initiative achievements of malaria elimination. However,there are still many imported malaria cases from abroad,and the species of the Plasmodium are diverse. Therefore,the imported malaria from abroad still remains the key of malaria control in Jiangsu Province.

O bjective:To investigate the effectiveness of D-xylose absorption test in evaluating the ab-sorptive capacity of the remnant small bowel in short bowel patients.M ethods:5 hr urine D-xylose ex-cretion,1hr,2hr,3hr serum D-xylose levels and AUC was compared between the short bowel patients(n=22) and the healthy control(n=50).The correlationship among the D-xylose absorptive capacity,the remnant small bowel length and area,and protein absorptive capacity were analyzed.For the 6 pa-tients receiving rehabilitative therapy,theirD-xylose and protein absorption capacities before and after the therapy were also stud ied.R esu lts:5hr urine D-xylose excretion,1hr,2hr,3hr serum D-xylose levels and AUC were all significantly lower in short bowel patients compared to control group(P