Objective To evaluate the influence of angiotensin converting enzyme (ACE) gene polymorphism on the effect of aerobic endurance training of soldiers.Methods Two hundred and forty-eight army recruits (males,Hans) from an infantry regiment without previous history of military training were involved in the present study,and they were divided into experimental group and control group (124 each).Intensified endurance training designed with the theory of "cyclic training" was applied in experimental group,and routine training method was applied in control group including mainly 5000 meters running for 3 times in one week (no more than 1 time per day).The 5000m running performance and maximal oxygen consumption (VO_2max) were determined before and after eight-week training as endurance capacity indices.The ACE gene polymorphism was determined and the recruits in both groups were divided into 3 sub-groups according to the ACE genotypes as II,ID and DD.The influence of ACE genotype and training method on endurance capacity was analyzed.Results The 5000m running performance and VO_2max of experimental group after eight-weeks-training were significantly higher than those before training and those of control group (P0.05).In control group,the 5000m running performance and VO_2max of DD sub-group were significantly lower than those of II sub-group and ID sub-group (P0.05).Conclusions Intensified endurance training designed with the theory of "cyclic training" may effectively improve the endurance capacity in 8 weeks.ACE genotype could not be used as an accurate indicator to evaluate the endurance capacity and improvement of efficiency of soldiers.

Objective To explore an appropriate training method to quickly improve the soldiers' aerobic endurance capacity and decrease the incidence of military training injuries for combat troops. Methods Two hundred and forty-eight recruits (males,Hanzu) from infantry without previous history of military training were involved in the present study,and they were divided averagely into experimental group and control group (124 each). Intensified endurance training designed with the theory of "cyclic training" was applied in experimental group,and routine training method was applied in control group including mainly 5000 meters running (no more than once a day,3 times a week). The resting pulse,vital capacity,maximal oxygen consumption (VO2max) and the performance of 5000m running were determined before and after eight-week-training as endurance capacity indices. Physical examination was performed to count the cases of military training injuries including calf swelling and tenderness,knee swelling and pain,and low back pain,and then the incidence of military training injuries was calculated. Results In the experimental group,the resting pulse decreased by 8.0%,while the vital capacity,VO2max and 5000m running performance elevated by 8.1%,20.0% and 14.7% respectively after training,which were significantly different in comparison with that before training and of control group (P

Objective To develop a mobile terminal data acquisition for data collection of malaria prevention and control in the field. Methods Based on 3G,Bluetooth,GPS and/or Bei Dou Navigation Satellite System(BDS)and wireless network communication technology(WIFI),a mobile terminal data acquisition for data collection of malaria prevention and control was developed. Results The developed system included 2 functional modules:a terminal and iCloud,and had examination,modi?fication and deletion functions of malaria epidemiological data. By using GPS and GIS,the target population or institution could be accurately positioned to determine whether the distribution of malaria cases had a spatial aggregation. Conclusion A mobile terminal data acquisition in malaria prevention and control is successfully developed,which is very convenient for data collec?tion and submission of malaria prevention and control.

Objective]Not only to promote the rational allocation of resources between government, society and universities,but also to provide theoretical basis and practical path for connotational development of newly-built undergraduate college.[Methods]Focusing on“ the development situation and the demand for the connotational development of the newly-built undergraduate college”and“ the aid possibility”,five representatives of newly-built undergraduate colleges and seven aid-donors were interviewed and inquired by Likert scale .[Results]The average index of newly-built undergraduate colleges on“ the development situation” and “ the demand for the connotational development” are respectively 2.93 and 4.03,showing a good situation and strong demand for external assistance.Meanwhile, the aid-donors also show a high will on the aid possibility” ,the average index is 4.06.[Conclusion]Based on analysis of“ the development situation and the demand for the connotational development of the newly-built undergraduate college, andthe aid possibility”,our research puts forward aid model and mechanism in connotational development of newly-built undergraduate college in order to make full use of external assistance, including government,society and universities.

Objective To analyze the clinical efficacy of lateral elbow approach combined with anterior median approach in the treatment of terrible triad of elbow.Methods Retrospectively analyzed 20 patients of terrible triad elbow who were admitted into our hospital from April 2012 to January 2015 and treated with lateral elbow approach combined with anterior median approach.The postoperative recovery of arthrosis,forearm rotation range,and range of motion were evaluated by Mayo score system.Results All patients wounds were with primary healing.There were 11 cases of excellent and 4 cases of good through Mayo score system,with the good rate of 75％.At the final follow-up,the range of motion was (122.06 ± 24.17) ° and the forearm rotation was (136.28 ± 8.9) °.Conclusion Lateral elbow approach combined with anterior median approach for the treatment of terrible triad of elbow has clear visual field,excellent surgical rate,without complications such as elbow instability and nerve damage.

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Objective To study the effect of relaxation therapy on sleeping quality of patients with post cran- iocerebral traumatic syndrome. Methods 90 cases with sleep disorders were randomly divided into the treatment group and the control group. The control group was given routine care and the treatment group was given relaxation therapy combined with routine care. Take pittsburgh sleep quality index (PSQI) as evaluation criteria. Results There was significant difference between the two groups( P < 0.01 ). Sleep quality was more improved by relaxation therapy in the experimental group than that of the control group. Conclusion Relaxation therapy can effectively im- prove sleep quality in patients with post craniocerebral traumatic syndrome.

Objective: To investigate the relationship of murine fibrinogen-like protein 2(mfgl2) / fibroleukin with pulmonary impairment in the murine model of severe acute respiratory syndrome(SARS).Methods: The Balb/cJ mice infected with murine hepatitis virus strain 3(MHV-3) through the trachea were observed for the pathological features and virus distribution in different organs.The expressions of both mfgl2 and fibrino in the lungs were determined by in situ hybridization and immunohistochemistry.Results: The MHV-3 infected mice developed typical interstitial pneumonia with extensive hyaline membrane formation in the alveoli,presented micro-vascular thrombosis in the pulmonary vessels and died within 5 days.MHV-3 virus replication was identified in all the organs observed.The specific expression of mfgl2 prothrombinase was noted in the terminal and respiratory bronchioles,alveolar epithelia and infiltrating cells.Conclusion: The characteristics of the pulmonary impairment of SARS in human can be properly simulated by the MHV-3 induced murine model of SARS.The up-regulation of the specific gene mfgl2 in the lungs involved in fibrino deposition and microvascular thrombosis may be largely responsible for SARS-associated pulmonary damages.

Objective To investigate the effect and clinical valve of target-controlled infusion of remifentanil for conscious intubation of patients under fibrobronchoscopy.Methods Forty patients were randomly divided into two groups(F and R),each group contained 20 cases.All of the patients were intubated on regional anaesthesia,as the first step,then the patients in F group were given fentanyl 2?g/kg by vein.In remifentanil target-controlled infusion group(R group),the patients were kept infusing remifentanil 2ng/ml.Results (1)Haemodynamics:in R group,SBP and HR kept steadily;In F group,while the two indices increased significantly.(2)Static pulmonary function:in R group,RR,VT deceased remarkablely.There were no difference between two groups in SpO_2 and P_ ET CO_2.(3)Tracheal intubating condition and Ramsay score in R group were better than in F group;Cardiovascular response in R group was lower.Conclusion Target-controlled infusion of remifentanil for conscious intubation can achieve excellent result,with less cardiovascular response.

Objective To determine the inhibitory effect of a synthetic hexa-peptide GGWSHW (G peptide) derived from thrombospondin-1 (TSP1) on TGF-? activation induced by angiotensin Ⅱ (Ang Ⅱ) in cultured human renal tubular epithelial cells. Methods Human proximal tubular epithelial cell line (HK-2) was cultured in vitro, untreated HK-2 cells were acted as normal control group. HK-2 cells were then stimulated by Ang Ⅱ for 1-24 hours (Ang Ⅱ stimulation group),so that optimal dosage and duration could be chosen.One hour prior to induction,HK-2 cells were pretreated with 10 ?mol/L peptide G (G peptide treated group)or losartan (losartan treated group), the blocker of I type receptor of Ang Ⅱ was acted as inhibitory control.The mRNA and protein levels of TSP1,TGF-?1,FN and PAI-1 were measured by RT-PCR and Western blot. Confocal microscopy and flow cytometry were performed to detect the presence of TSP1, TGF-?1 and co-positive expression of two protein, respectively.The concentrations of total and active TGF-?1 as well as FN and PAI-1 in cell culture supematants were measured by ELISA. Additionally, the expression of Smad2 and p-Smad2 was also examined for the bioactivity of TGF-?1 signaling protein. Results Ang Ⅱ enhanced the expression of TSP1 and TGF-?1 in a temporal-spatial dependent manner. The optimal dosage and duration were 1 ?mol/L and 6 hours,for TSP1,and 0.1 ?mol/L and 12 hours for TGF-?1 respectively.Comparing with untreated HK-2,the co-expression of TSP1 and TGF-?1 induced by A Ⅱ showed a increase of 5.4 folds.In addition,the protein level of p-Smad2 was elevated remarkedly, the mRNA level of FN and PAI-1 was up-regulated by 3 and 1.5 folds, and the concentration was increased by 2.0 and 1.9 folds respectively. Peptide G had less effect on the expression of TSP1 and TGF-?1,whereas it significantly reduced the secretion of active TGF-?1,though total level of TGF-?1 remained up-regulated. Furthermore,comparing with losartan treated group, p-Smad2 expression was reduced by 28.9%, the mRNA level of FN and PAI-1 was decreased by 34.5% and 26% respectively,and the protein levels were reduced by 11.0% and 8.9% respectively. Conclusion The inhibitory peptide derived from TSP1 effectively suppresses TGF-?1 activation through a competitive mechanism and also reduces the secretion of FN and PAI-1 associated with fibrosis.