Objective:To investigate the expression of recombined human immunoglobulin ? light chain 022 and TOM1 gene and to determine the protein expression of HSIGVL022 in omental adipose tissues of patients with type 2 diabetes and insulin resistance(T2DM-IR).Methods: Fat tissues from greater omentum of T2DM-IR patients and normal controls were obtained.The mRNA levels of recombined human immunoglobulin ? light chain 022 and TOM1 were measured by fluorescent quantitative RT-PCR;expression of HSIGVL022 protein was identified by immunohistochemistry.Results: The mRNA levels of HSIGVL022 and TOM1 in T2DM-IR patients were(34 140?6 160) copy/million house-keeping genes and(4 440?617) copy/million house-keeping genes,respectively;those in control group were(5 930?661) copy/million house-keeping genes and(1 360?82) copy/million house-keeping genes,respectively.There were significant difference between the 2 groups(P

Objective To study the chemical constituents of the medicinal fungus Shiraia bambusicola.Methods Chromatography on silica column and preparative HPLC were used to purify the compounds;Spectroscopic methods including 1H-NMR,13CNMR,and MS were used to elucidate their structures.Results Nine compounds were isolated and identified as 3,6,8-trihydroxy-l-methylxanthone(1),3,8-dihydroxy-6-methoxy-1-methylxanthone(2),2,3,6,8-tetrahydroxy-l-methylxanthone(3),3,4,6,8-tetrahydroxy-l-methylxanthone(4),pregn5(10)-en-3?,17?,20?-triol(5),macrosphelide A(6),(+)-griseofulvin(7),griseophenone A(8),and 11,11′-dideoxyverticillin A(9).Conclusion Compounds 1-9 are obtained from the fungus S.bambusicola for the first time.

Objective To measure the scope of normal meniscus by magnetic resonance imaging(MRI),and create a database of meniscus size of each part.Methods The MRI image measured rake angle and back angle,body height and width of each part and the angular distance of rake and back angle of 303 meniscus of normal knee joint.Results The width and height of anterior horn,anterior exterior angle,body,angulus posteriolateralis and back angle of the medial meniscus were (5.67 ± 1.37) mm and (3.53 ± 0.86) mm,(7.24 ± 1.91) mm and (4.38 ±1.01) mm,(5.61 ± 1.73)mm and (4.25 ±1.07) mm,(11.01 ±3.53) mm and (5.41 ± 1.66)mm,(9.21 ±2.71)mm and (5.02 ± 1.39)mm,respectively.Rake and back angle of angular distance was (38.51 ± 10.88)mm.The width and height of the anterior horn,anterior exterior angle,body,angulus posteriolateralis and back angle of the lateral meniscus were (6.95 ± 1.69) mm and (3.83 ± 0.91) mm,(7.48±1.90)mmand (4.06 ± 0.97) mm,(6.51± 1.79) mm and (5.10±1.01)mm,(7.62±1.99)mm and (5.18±1.19)mm,(6.68 ± 1.41) mm and (5.64 ± 1.12) mm,respectively.The rake and back angle of angular distance was (38.51 ± 10.88) mm.Conclusion In this study,we obtained the data of meniscus for normal people by MRI,which provides reference values for diagnosis of meniscus injury and transplantation.

Objective To observe the effect of Fuzheng Hunao capsule on TNF-? and HSP70 protein expression of myocardial tissues in rats with intracerebral hemorrhage.Methods Seventy two rats were randomly divided into 4 groups:sham,model,Fuzheng Hunao and Angong Niuhuang.The protein expression of TNF-? and HSP70 in rat myocardial tissues were detected by the immunohistochemistiy method,and the integrated optical density (IOD) was measured with image analysis.Result The IOD of TNF-? and HSP70 in model group was higher than that in sham group.The IOD of TNF-? in Fuzheng Hunao and Angong Niuhuang group was lower than that in model group.While the IOD of HSP70 was higher than that in model group.Conclusion Inflammatory response attend the myocardial damage.Fuzheng Hunao capsule could protect the myocardial tissues by inhibiting the protein expression of TNF-? and increasing that of HSP70.

Objective To evaluate the clinical efficacy of primary intracoronary stenting in the infarct related artery in elderly patients after acute myocardial infarction(AMI). Methods Percutaneous transluminal coronary angioplasty(PTCA) plus primary coronary stenting was performed for 41 AMI patients(≥70 years) within 12 hours from the onset of chest pain. Infarct related arteries were left anterior descending artery in 21 patients(51 2%), left circumflex in 7(17 1%), right coronary artery in 13(31 7%). Thrombolysis in myocardial infarction (TIMI) showed that blood flow was grade 0 in 29 patients, grade 1 in 7, grade 2 in 5. Results Forty eight stents were implanted in the infarct related artery for 41 patients. One patient died of cardiac shock during the procedure, and the success rate was 97 6%(40/41). Grade 3 of blood flow after TIMI was gained in 37 patients(90 2%). One patient died of renal failure while hospitalizing. The clinical successful rate was 95 1%(39/41). Thirty six patients were followed up, 1 patient with restenosis of infarct related artery was performed cutting balloon angioplasty, and 1 patient with congestive heart failure was rehospitalized. Other 34 patients did not manifested angina pectoris. Conclusions Primary coronary stenting for the infarct related artery in elderly patients after AMI is safe and effective with a high successful rate and less complications.

Objective To identify stent fracture by revision of coronary artery angiography and intravascular ultrasound(IVUS) images.Methods Stent fracture has been implicated as a cause of drug-eluting stent failure.IVUS is more likely to identify stent failure(including stent fracture) when compared with angiography.We analysed retrospectively 2 021 cases of angiography and 183 cases of IVUS.Results Six fractures were found in 3 patients by angiography and five of these fractures were confirmed by IVUS in 2 patients.All stent fractures occurred in sirolimus-eluting stents in less than one year after implantation.Angiography analysis showed that 4 stent fractures were complete transverse linear fracture with stent displacement.IVUS analysis showed that 3 stent fractures were complete and 2 were partial fracture.One fracture site was found at the overlapping point of stents and one was found with coronary aneurysm.Conclusion Angiography and IVUS are helpful to identify stent fracture.IVUS can help to understand the possible mechanisms of stent fracture.

AIM:To explore clinical effect of Zhongfeng Fangzhiling Granule(Radix Pseudostellariae,Radix Polygoni Multiflori praeparata cum succo glycines sotae,Hirudo,etc.) on acute cerebral thrombosis.METHODS:72 patients were randomly divided into 2 groups.The treatment group was treated with Zhongfeng Fangzhiling Granule,the control group was treated with Zhilong Xuetong Capsule.The treatment effects between two groups were compared.RESULTS:The effect in treatment group was superior to that in control group.CONCLUSION:Zhongfeng Fangzhiling Granule has significiant therapeutic effect on patients with acute cerebral thrombosis,and the preparation method is simple,so it will be a prospective herb granule.

0 05) One patient of group A had in stent thrombosis after procedure Fifty one patients of group A and 41 of group B were followed up (from 1 to 39 months) after procedure, and 48 of group A and 37 of group B without cardiac events, such as death, AMI 3 patients of group A and 4 of group B were repeated coronary angiography because of chest pain There were 1 patient with restenosis and 2 with severe stenosis in non infarct related artery in group A, and 1 with restenosis and 3 with severe stenosis in non infarcted related artery in group B Conclusion Compared with short stenting in short lesions, long coronary stenting in long or diffuse infarct related artery disease after AMI is the same as a high procedure success rate, low complication and good clinical outcomes

Objective The purpose of this study was to determine the feasibility and efficiency of direct stent implantation with intravascular ultrasound (IVUS) guidance Methods 12 native vessel target lesions in 12 patients were studied by coronary angiography and direct stent implantation Ultrasound imagines obtained at the treatment site before and after stenting were analyzed quantitatively for in lesion vessel wall diameter, in lesion minimal lumen diameter and in lesion vessel length Direct stent deployment was performed with IVUS guidance The ends of follow up were acute or subacute thrombosis and major cardiac events (MCE: cardiogenic death, angina pectoris, myocardial infarction and revascularization correspondence with target vessel) Results Direct stenting was successfully performed in 12 patients with IVUS guidance Coronary angiography showed that there was no residual stenosis and TIMI 3 flow was obtained in all patients Further balloon dilation of the stent was performed by observation of the IVUS imagines in two patients The final IVUS imagines showed that ratio of minimal lumen diameter/maximum lumen diameter ≥0 7 and in lesion minimal lumen diameter increased from 1 2?0 87 mm to 3 4?2 8 mm No thrombosis and MCE were found in all patients in 1 4 months follow up Conclusion Direct stenting with IVUS guidance in selected patients had higher success rates, less complications and better clinical results

Objective To establish the quality standard of Heibu ointment. Method The contents of Galla Halepensis were determined by TLC, and Gallic Acid was determined by HPLC. Results In TLC, the spots were very clear and specific to identify Heibu ointment. The linear range of Gallic Acid was 0.1～1.1 ?g. The quality standard of Heibu ointment was established. Conclusion The method can be used for quality control of Heibu ointment.