Objective To investigate the significance of changes of serum high-sensitivity C-reactive protein (hs-CRP),tumor necrosis factor-alpha (TNF-α),interleukin 6 (IL-6) and urinary microalbumine levels in patients with diabetic kidney disease.Methods Serum hs-CRP,TNF-α,IL-6 and urinary microalbumine levels were measured with RIA in 60 type 2 diabetic patients and 20 controls.The 60 diabetic patients were divided into three groups based on urine albumin excretion rate.Group A:without alblminuria ( UAER ＜ 20 μg/ min,n =20 ) ; Group B:micro-albuminuria ( UAER ＜ 200 μg/min,n =20 ) ; Group C:macro-albuminuria ( UAER ＞ 200 μg/min,n =20 ).The serum levels of hs-CRP,TNF-α,IL-6 of patients in the four groups were measured.Results The serum levels of hs-CRP,TNF-α,IL-6 of patients in the healthy group were ( 1.21 ± 0.87 ) mg/L,( 1.27 ± 0.93 ) ng/L and ( 4.31 ± 1.72 ) ng/L,in the nonalbuminuria group were ( 2.31 ± 1.07 ) mg/L,( 1.95 ± 1.34 ) ng/L and ( 5.79 ± 1.68 ) ng/L,in the microalbuminuria group were ( 3.47 ± 1.25 ) mg/L,(2.86 ± 1.26) ng/L,(7.13 ± 1.57 ) ng/L,in the macroalbuminuria group were (4.83 ± 1.47 ) mg/L,(4.03 ±1.43)ng/L,(9.14 ±2.43)ng/L Serum levels of hs-CRP,TNF-α and IL-6 were significantly higher in all the patients with type 2 diabetes than those in the control group (P ＜ 0.01 ).Serum levels of hs-CRP,TNF-α and IL-6 were significantly higher in type 2 diabetic patients with microalbuminuria than those in patients without albuminuria (P ＜0.05).Serum levels of hs-CRP,TNF-α and IL-6 were significantly higher in diabetic patients with macroalbuminuria than those in patients with micrealbuminuria (P ＜ 0.01 ).The levels of hs-CRP,TNF-αand IL-6 were positively related to the urinary microalbumine levels ( rs =0.67,0.64,0.75,P ＜ 0.01 ).Conclusion Determination of serum hs-CRP,TNF-α and IL-6 levels might be helpful for early detection of diabetic kidney disease and monitoring the progress of the disease process.

Ten healthy mongrel dogs of either sex were divided into two groups: Dex-treated group (n=6) and NS-treated group (n=4). The dogs were attacked with hemorrhagic shock by bleeding via left femoral artery. Dex-treated group was given Dexamethasone (5mg/kg) at mean arterial pressure (MAP) of 13.3 kPa. NS-treated was given placebo of 0.9% Nad solution. The atrial natriuretic peptide (ANP) was determined by radioimmunoassay. Plasma level of ANP decreased progressively 5 h after shock. There was a significant positive correlation between the extent of reduction of MAP and that of plasma ANP (r=0.871203, n=16, P

Objective To discuss the influence of different dose α-lipoic acid on blood glucose and C-reaction protein(CRP) in type 2 diabetic patients with macroangiopathy.Methods Seventy-two cases type 2 diabetic patients with macroangiopathy were divided randomly and voluntarily into the conventional dose group(n=36) and high dose group(n=36).The patients of conventional dose group were given conventional dose α-lipoic acid(300 mg/d) based on glucose treatment while high dose group were given high dose α-lipoic acid (600 mg/d) based on glucose treatment,the courses were all 15 d.The fasting blood glucose(FBG),2 h postprandial blood glucose(2 hBG),serum CRP of before and after treatment of between the two groups and safety during the treatment were compared.Results The FBG,2 hBG and CRP of before and after treatment were respcetively (9.24±2.18) mmol/L and (8.18±1.38) mmol/L,(13.26±3.17) mmol/L and (11.26±1.63) mmol/L,(21.52±4.17) mg/L and (14.72±3.73) mg/L;(9.26±2.04) mmol/L and (5.82±1.27)mmol/L,(13.52±2.45) mmoL/L and (8.92±1.04) mmol/L,(22.65±4.67) mg/L and (8.61±1.52) mg/L,all groups decreased obviously,the differences were statistically significant compared to that of before treatment(t=4.265,4.654,4.956 and 5.562,6.254,7.654,P＜0.05),and the high dose group showed more lower (t=5.353,5.783,6.257,P＜0.05).After treatment,ALT ((37.26±9.64) U/L),AST ((38.22±7.04) U/L),BUN((7.25±1.52) mmol/L) and Scr((55.25±11.25) mmol/L) of all groups increased obviously than pretreatment((33.53±6.37) U/L,(33.15±7.16) U/L,(5.43±4.67) mmol/L,(53.15±13.65) mmol/L),but the differences were no statistically significant compared to that of before treatment (t=-2.061,-2.165,-1.455,-0.689,P＜0.05),and there were also no statistical significance between the two groups(t =2.125,3.026,1.235,1.035,P＜0.05).Conclusion α-lipoic acid can obviously decrease the blood glucose and CRP in type 2 diabetic patients with macroangiopathy,which has a significant dose effect relationship,and is safe and worthy of clinical application.

Objective To discuss the effect of alprostadil combined with irbesartan in patients with early diabetic nephropathy.Methods Ninety-six patients with early diabetic nephropathy were divided randomly and voluntarily into the control group (n =48) and observation group (n =48).All patients were given regular treatment,the patients of the control group were given irbesartan (150 mg,oral) based on regular treatment while the observation group were given alprostadil(intravenous drip alprostadil 10 g with saline 250 ml,1 times a day)based on treatment of the control group,the course was four weeks.The blood rheology,serum albumin,24 hours urinary albumin excretion rate (UAER) and urea nitrogen (BUN),serum creatinine (Scr) and clinical effect between the two groups were compared.Results After treatment,the whole blood midst shear viscosity values,whole blood low shear viscosity values and whole blood high shear viscosity values of observation group were respectively (5.32±1.16) mPa · s,(8.73±2.15) mPa · s and (3.52±0.52) mPa · s,significantly lower thanof control group((6.74± 1.92) mPa · s,(9.96±2.43) mPa · s,(4.53±0.64) mPa · s),the differences were statistically significant (t =4.627,4.728,4.226;P＜0.05).The Alb of observation group was (36.36±7.24) g/L,obviously higher than that of control group (29.73 ± 5.36) g/L,the difference was statistically significant (t=5.336,P＜0.05),while the UAER,BUN and Scr of observation group were respectively (375.84±24.24)mg/L,(9.61 ± 1.42) mmol/L and (136.36 ± 26.73) μmol/L,obviously lower than that of control group ((636.42± 43.56) mg/L,(13.45 ± 3.14) mmol/L,(176.32 ± 41.62) μmol/L),the differences were statistically significant (t =6.738,4.628,6.662;P＜ 0.05).The total effective rate of observation group was obviously higher than that of control group,the differences were statistically significant (93.75％ (45/48) vs.66.67％ (32/48);x2 =6.637,P＜ 0.05).Conclusion Alprostadil combine with irbesartan can effectively improve the blood rheology and renal function in patients with early diabetic nephropathy and improve the living quality,the effect is better than irbesartan only,it is worth popularization and application.

Objective To observe the clinical effect of mifepristone combined with intramuscular injection of methotrexate in the treatment of cesarean scar pregnancy.Methods Fifty-six patients with uterine scar pregnancy after cesarean section were divided into observation group and control group by random number method.The control group was intramuscularly injected methotrexate,the observation group was given methotrexate combined with mifepristone therapy.The therapeutic effect,blood β human chorionic gonadotropin(β-HCG) decreased to normal time,vaginal bleeding time,mass size were observed.Results After treatment,the serum β-HCG decreased to normal time[(15.96±3.71)d] and vaginal bleeding time[(5.12±2.08)d] of the observation group were significantly shorter than those of the control group [(25.42±4.02)d,(7.39±3.37)d],the mass size of the observation group [(1.88±0.15)cm] was also significantly smaller than (2.14±0.33) cm of the control group,and there were significant differences between the two groups(t=4.3142,3.0331,3.7954,all P＜0.05).Conclusion Mifepristone combined with intramuscular injection of methotrexate has significant effect on uterine scar pregnancy after cesarean section.

Objective:To investigate the effects of electroacupuncture (EA) on intestinal motility disorder in rats with irritable bowel syndrome (IBS) in order to provide experimental evidence for improving clinical treatment to IBS with EA. Methods: IBS was induced by inserting a balloon into the rectum of male Sprague-Dawley rats from their neonatal age (8th, 21st day after birth) and applying colorectal distention stimulation by inflating air into the balloon. Peristaltic wave (PW) was used to estimate intestinal motility. Rats were divided into 4 groups (n=6): normal group, untreated group, sham EA group and EA group. Acupoints of Zusanli (ST36) and Shangjuxu (ST37) in the hind limbs bilaterally were chosen for EA and sham EA treatment. Trains of dense-sparse frequencies (100 Hz and 2 Hz alternately) and intensity of 1, 2 and 3 mA (10 min for each) were applied for 30 min with each EA treatment while inserting similar needles without electrical stimulation was done as sham EA treatment. PW recorded for 30 min in normal and untreated groups respectively was taken as control values, while PW recorded after EA or sham EA treatment for 30 min was taken as responsive values. Results: Frequency of PW was significantly increased in the untreated group as compared with that in the normal group (P<0.05), and decreased after 30-min EA treatment (P<0.05). There was no significant change in the PW of sham EA group as compared with the control group and before treatment (P>0.05). Conclusion: These results suggest that 1) there is an abnormal increase in intestinal motility indicating an intestinal motility disorder in IBS rats; 2) EA but not sham EA can relieve such intestinal motility disorder.

Hepatocellular carcinoma(HCC) is one of the most common malignant tumors in the world.According to the statistic data of Ministry of Public Health of China in 1995, the annual mortality of HCC was 20.4 per 0.1 million, and had the rise tendency. Surgery is commonly the main modality of HCC combined therapy. The high intensity focused ultrasound (HIFU), as a noninvasive therapy, with the mechanism of transient high temperatures, cavitation and mechanical effects, has been recognized. This review aimes to elucidate the immune changes in HCC after HIFU exposures and the mechanism.

Long non-coding RNAs are important regulators of gene expression.ANRIL which was coded on the Chr9p21.3 loci participates in the pathogenesis of tumor, coronary artery disease, type 2 diabetes mellitus and oth-er diseases.Multiple ANRIL isoforms are tissue-specific.ANRIL mainly functions through Polycomb proteins, while there are also other downstream targets.The mechanism of each isoform and the downstream pathways are hotspots incurrent researches.

Objective: To investigate Leptin resistance of patients with metabolic syndrome. Methods: 50 patients with metabolic syndrome and 30 health persons as control were investigated. Body mass index(BMI)、waist to hip ratio(WHR)、blood pressure(Bp) were recorded. Serum Leptin、plasma glucose、insulin、total cholesterol(TC)、triglyceride(TG)、high density lipoprotein cholesterol(HDL C)、low density lipoprotein cholesterol(LDL C)、lipoprotein(a) were determined,and HOMA IR was calculated. Results: Compared to controls, serum Leptin concentration was significantly higher in metabolic syndrome〔(23.82?15.63)?g/L vs (11.20?4.70)?g/L, P

Objective:To discuss the effect of optimum program on hypertensive cerebral hemorrhage in acute phase.Methods:380 patients with hypertensive cerebral hemorrhage were selected.They were randomly divided into test group by given two agents(Xuezhongxiao/Zhongfengfangzhiling)+comprehensive rehabilitation(rehabilitation of modern acupuncture)+ medicine-based treatment +microinvasive surgical therapy,and control group by given Chinese medicines(Xuekang /Naoan capsule) + modern rehabilitation+ medicine-based treatment + craniotomy treatment.The curative effects was observed and compared between the two groups.Results:The test group was evidently superior to the constrol one in haematoma clearance rate,nerve functions recovery,extremity rehabilitation and symptoms improvement(P