Objective:To investigate the complication of microinvasive surgery for osmidrosis and find ways of preventing and treatment.Methods:284 osmidrosis patients undergoing minimally invasive surgery were retrospectively analyzed,the reasons and presentations of the complication were reviewed.Results:Complications occurred only by 13%,including haematoma,epidermal necrosis,scan,recurrence and nerve injury.Conclusion:Microinvasive surgery is a new technique for treating osmidrosis.The skilled operation makes success of the surgery,so the doctors should get hard training for the microinvasive surgery in order to reduce the complications and obtain good results.

Objective: To explore the possible factors that affect the mental health of military officers and soldiers in Tibet and provide the basis for the prevention of mental disorder and the practice of mental intervention. Methods: 90 Symptoms Check List (SCL-90),Life Events Scale(LES) and self-made questionnaire for general condition were used to investigate 299 army officers and soldiers who stationed in the frontier post of Tibet. The subjects were chosen in the principle of cluster sampling in squad or platoon. Results: The factors of environment satisfaction, negative live events, ways of entering Tibet, total score of life events, army years and age were positively related with SCL-90, while the hometown living condition had significantly negative relation with SCL-90. The factors of environment satisfaction, negative life events ,army years, motivation of joining the army, ways of entering Tibet and hometown living condition were in the regression equation(P

BACKGROUND:Pierre Robin Sequence is a congenital malformation which is characterized by micrognathia, glossoptosis and respiratory tract obstruction with or without cleft palate. SOX9, KCNJ2, Ptprs and Ptprf are probably connected with Pierre Robin Sequence. OBJECTIVE:To review the recent progress in the researches on the related genes about Pierre Robin Sequence. METHODS:A computer-based online search of CNKI database and PubMed database was performed to retrieve the relevant articles published from January 1999 to September 2014 with the key words of“micrognathia, Pierre Robin Sequence, mutation, gene locus”in Chinese and English, respectively. Final y, 58 articles were included for review after deleting unrelated and repetitive ones. RESULTS AND CONCLUSION:SOX9, KCNJ2, Ptprs and Ptprf are probably connected with Pierre Robin Sequence. Recently, the research on the genes connected with Pierre Robin Sequence focuses on 17q23-24, and smal sample cases are commonly seen. But, further large sample test and case analysis, as wel as related animal models are needed to analyze the role of these genes in the pathogenesis of Pierre Robin sequence, as wel as consequently, we can analyze the etiology and pathogenesis of Pierre Robin sequence.

Objective To investigate the effect of isoflurane preconditioning on Bcl-2 and Bax mRNA expression and the signaling pathway of nitric oxide against brain ischemia-reperfusion(I/R).Methods Seventy-five male gerbils were randomly divided into 5 groups(n=15):SHAM,I/R,ISO,AG+ISO and AG group.Global cerebral I/R was produced by occlusion of bilateral common carotid arteries for 5 minutes and confirmed by isoelectric potential on EEG.In ISO group,the animals inhaled 1.2%-1.5% isoflurane for 30 minutes followed by 30 minutes wash-out period before I/R.In AG+ISO group,30 minutes after aminoguanidine,200 mg/kg i.p.isoflurane was inhaled as ISO group,and aminoguanidine was injected 30 min before I/R in AG group.After 24 hours,the forebrains were collected,and the expression of Bcl-2 and Bax mRNA was determined by reverse transcriptase polymerase chain reaction.Results Bcl-2 mRNA expression in ISO group(1.22±0.12) was significantly higher than that in sham(0.83±0.16),I/R(0.83±0.11) and AG+ISO group(0.82±0.12)(P<0.05).Bax mRNA expression in I/R and AG group was significantly higher than that in sham group(P<0.05),and the expression in ISO group was higher than that in AG+ISO group.Conclusion Nitric oxide is involved in the signaling pathway of isoflurane preconditioning and has the protection effect on gerbil brain against I/R.

Objective:To investigate the regulatory mechanism of LBP on T lymphocytes excessive apoptosis and the mRNA expression of apoptotic genes in aged rats Methods:T lymphocyte apoptosis and the expression of pre apoptotic and anti apoptotic genes (Fas, FasL, TNFR1, Bax, Bcl2 and TNFR2) from young rats and old rats treated with or without LBP were studied using flow cytometry and fluorescence real time quantitative RT PCR Results:LBP could effectively downregulate the percentage of T cell apoptosis in aged rats At the same time LBP also downregulated the transcription level of TNFR1 gene and upregulated the transcription level of Bcl 2 in T cells from old rats Conclusion:The T cell excessive apoptosis in old rats could be effectively downregulated with LBP, by the by of decreasing the expression of pro apoptotic gene and enhancing the expression of anti apoptotic gene

Objective To evalue the effects of elevation epidural block with 0.2% ropivacaine on lung function. Methods fifty ASA Ⅰ-Ⅱ patients undergoing elective radical mastectomy were anesthetized with method of epidural block .T 2-3 or T 3-4 were selected as puncture site. A catheter was inserted into the epidural space in a cephalad direction for 3-4cm. Nasal catheter oxygen inhalation was used in routine method. A loading dose of 0.2% Ropivacaine 15-20ml was given after a test dose of 1% lidocaine 5ml or 0.2% ropivacaine 5ml. Generally, the first dosage was at 25-30ml. The anesthesia effect, segments of block were measured and heart rates (HR),blood pressure (BP), blood oxygen saturation(SpO 2) were assessed before anesthesia, after anesthesia and at the end of surgery, meanwhile the breathing frequency, minute ventilation and maximal voluntary ventilation(MVV) were monitored. Blood gas, lactic acid and serum glucose were detected from 25 of the total case before and after anesthesia. Levels of sense obstacle and movement of bilateral upper limbs were recorded after operation. Results All 50 patients but one were finely anesthetized by elevation epidural block with 0.2% ropivacaine. Among them 39(78%) patients were blocked at C 5-T 8. No significant difference existed before and after anesthesia on breathing frequency, minute ventilation and maximal voluntary ventilation, and the values of blood gas in all those patients analysed were normal. Over 20% of decline on BP and obvious reduction on HR were found in 8 patients after anesthesia (P

Objective To investigate the effect of transabdominal gastroesophageal stapling in management of variceal bleeding in portal hypertension with Hassabi operation and sum up our clinical experience.Methods Retrospective analysis of 48 cases with portal hypertension operated by transabdominal gastroesophageal stapling between May,1998 and Oct.2005.The elimination of oesophagus venous、the rebleeding rate、the operation mortality、the stricture and the fistula rate and the index that influences the liver function were maily observed.Results No case suffered rebleeding and hepatal encephalopathy during the hospitaligation of the patients.Esophageal varices disappeared or reduced in all cases.One case resulted from stricture and one case died of multiple organ failure.Conclusions Transabdominal extensive oesophagogastric devascularization combined with gastrooesophageal stapling is an effective and safe procedure for control of acute variceal haemorrhage with satisfactory short-term control,but the long-term effect needs to be long follow-up for the patients.

Objective To investigate the mRNA expression of secondary lymphoid tissue chemokine (SLC) in human gastric carcinoma. Method The study was designed to investigate semi-quantitatively the expression of SLC mRNA in gastric carcinoma and metastatic lymph nodes by reverse transcription-polymerase chain reaction.Result The expression of SLC mRNA in the gastric cancer tissues was markedly reduced compared with that in adjacent noncancer tissues. The average tumor/normal (T/N) ratio determined by RT-PCR was 0.43?0.08 in 29 patients.Metastatic lymph node/normal ratio was 0.63?0.07.Negative lymph node/normal ratio was 1.19?0.11. As a control,the mean G3PDH T/N ratio was 1.16?0.06,there was no correlation between SLC mRNA expression and clinicopathological characteristics in gastric cancer.ConclusionSLC mRNA expression is suppressed in the gastric tumours. SLC may play an important role in the early stage of carcinogenesis.

Objective To observe the effect of Fuzhenghunao capsule on NE of the locus ceruleus and myocardial tissues in rats with intracerebral hemorrhage. Methods 72 rats were randomly divided into 4 groups:sham,model,Fuzhenghunao and Angongniuhuang. The content of NE were tested by the high performance liquid phase-electrochemical method at 6 h,24 h and 72 h of post-operation. Result NE was obviously increased in sham group,and decreased in two treatment groups. Conclusion Fuzhenghunao capsule could be used to inhibit the activation of locus ceruleus noradrenalin system and protect myocardial tissues by regulating NE content.

Objective To observe the clinical effect of Zhang point in subclavian vein puncture. Methods One hundred patients underwent deep vein catheterization were selected, ASA grade of Ⅱ- Ⅲ. The patients were divided into 2 groups according to puncture method with 50 cases each, the patients in group A used traditional puncturing method, and the patients in group B used Zhang point puncture method. The total success rate, success rate of the first trial and incidence of complication were recorded. All the patients received the bedside chest X-ray examination to observe the location of central venous catheter after surgery. Results The total success rate and success rate of the first trial in group B were significantly higher than those in group A:100%(50/50) vs. 88%(44/50) and 96%(48/50) vs. 76%(38/50), the incidence of complication was significantly lower than that in group A: 4% (2/50) vs. 20%(10/50), there were statistical differences (P<0.05). Conclusions Using of Zhang point is very simple in subclavian vein puncture catheterization, with a higher success rate and a less complication rate.