Objective To study the radiographic findings of anomalies of thoracic skeletion in patients with congenital heart disease.Methods Frontal and lateral chest films of 252 cases with congenital heart disease proved by operation were reviewed.Results The skeletal anomalies in 8 cases including generalized sternal prominence,sternal bowing,pouter pigeon breast,hemivertebrae and butterfly vertebrae of thoracic spine,and deformities of ribs were discovered.Conclusion The skeleton anomalies which are divided into primary and secondary types often occur in patients with congenital heart disease.

Objective To explore the effect of chlamydia pneumoniae(CP)infection on T lymphocyte subsets in patients with chronic obstructive pulmonary disease(COPD).Methods A total of 176 patients with acute exacerbations of COPD who were admitted to hospital from Jan.2001 to Jan.2005,and 46 healthy subjects were selected.Chlamydia pneumoniae-specific serum IgG,IgA,IgM antibodies were measured by microimmunofluorescence(MIF)test;the flow cytometer was used to analyze the frequency of CD+_3,CD+_4 and CD+_8 lymphocytes in patients with COPD and controls.Results The rate of acute chlamydia pneumoniae infection in COPD patients with acute exacerbations was 27.3% and that of chronic CP infection was 19.3%.They were all significantly higher than that in healthy subjects(P﹤0.01).The frequency of the CD+_3 and CD+_4 lymphocytes showed no difference in each group.The frequency of CD+_8 lymphocyte in COPD patients of chronic CP infection rose and the CD+_4/CD+_8 ratio was decreased in comparison with controls.On the other hand,the CD+_8 lymphocytes and the CD+_4/CD+_8 ratio showed no difference between chronic CP infection group,no CP infection group and controls.Conclusion The chlamydia pneumoniae infection is a rather frequent event in acute exacerbations of COPD,and it is an important reason to cause the disorder of cell-mediated immunity in COPD patients.The disorder of cell-mediated immunity may be involved in pathogenesis of COPD.

AIM: To identify the key factors responsible for drug resistance in different ovarian cancer cell lines using protein microarray system. METHODS: Six ovarian cancer cell lines were employed. The sensitivity of ovarian cancer cell line to common chemotherapeutic drugs was determined by using MTT assays. The expression of 78 cytokines and other factors was examined by using cytokine antibody array technology. RESULTS: Different ovarian cancer cell line responded to chemotherapeutic agents differently. The drug resistance was correlated with certain cytokine expression. Cell line SKOV3 was less sensitive to first line chemotherapeutic drug (ADM, CBPDA) and accumulated high amounts of GRO and TIMP-2 compared with other 5 cell lines. OVCAR4 cells were more resistant to second line chemotherapeutic drug (TAXOL, VP16) and had higher levers of IL-6 and IL-8 than IGROV1, OVCAR3 and OVCAR5. CONCLUSIONS: Among the most common excretive cytokines, increasing of GRO, IL-6, IL-8 and TIMP-2 might be related to drug-resistance of ADM and CBPDA in ovarian cancer cell, while IL-6 and IL-8 might also be related with drug resistance of TAXOL and VP16. The different types of ovarian cancer cell might have roughly similar excretive cytokines-induced mechanism of drug resistance.

Objective To probe the neuroprotective mechanism of Donepezil in hippocampus of mice with vascular dementia(VD).Methods The mice were subjected for reperfusion repeatedly on bio-lateral common carotid arteries by repeatedly clipping and injected sodium nitroprusside into the abdominal cavity to establish the imitative VD rat models.Then Donepezil was given by intragastric administration.The behavioral abnormalities were investigated by Y-type maze test.The expressions of N-methy1-D-aspartate receptor subunit proteins(NR1)in neuron of hippocampus were observed by immunohistochemistry technique,and the activities of glutathione peroxidase(GSH-PX)and catalase(CAT)were measured with dithio-bis-nitrobenzoic acid(DTNB)and ultraviolet spectrophotometry(UV)methods,respectively.Results Compared with VD control group,the performance records of learning and memory in Donepezil group were better(all P

Objective To study the effect of Atorvastatin on the level of serum high-sensitivity C-reactive protein(hs-CRP) and nitric oxide(NO) in patients with acute cerebral infartion(ACI). Methods 60 patients with ACI were randomly divided into the Atorvastatin therapy group (n=30) and the conventional therapy group (n=30). At the basic of conventional therapy, the Atorvastatin therapy group was treated with Atorvastatin 20 mg/d. Both groups were treated for 14 consecutive days. The levels of serum hs-CRP and NO were measured and the scores of neurological deficit (NDS) were evaluated before and after treatment. Results The levels of serum hs-CRP in both two groups after 14 d treatment were significantly lower than those before treatment ( all P

Objective To construct the eukaryotic expression vector containing short hairpin RNA (shRNA) targeting the inhibition of Smad3 gene segment in keloid fibroblasts (KFBs) and to investigate the effects on the expression of Smad7 in KFBs. Methods A couple of the most effective siRNA screened from former experiments were used to recombine the plasmids of Smad3 shRNA, and then Smad3 shRNA was transfected into KFBs by LyoVec TM. The expressions of Smad3 and Smad7 at different time points (0～9 d) were detected by RT-PCR and Western blotting. Results ① The recombinant Smad3 shRNA vectors were identified by RT-PCR and Western blotting. ② The expressions of mRNA and protein of Smad3 in KFBs decreased significantly in a time-dependent manner after transfection of Smad3 shRNA and reached the lowest point at 72 hours in the experimental group. Optical density analysis revealed a significant difference between the experimental group and the control group (P

Objective To observe the dynamic changes in collagen type Ⅰ and collagen type Ⅲ in rabbits with schistosomiasis japonica and the treatment effect of gamma interferon on the degradation of collagens in schistosomal hepatic fibrosis.Methods Each rabbit was infected with 80?1 S japonicum cercariae. Liver operations were done at different time points after infection and the liver specimens were embedded with paraffin and stained with ? SMA, HE and picric acid Sirius red. The stained slides were observed under polarizing microscope and different collagen areas calculated by computer imagine analysis system. At the 16th week after infection, the infected rabbits received a single dose of praziquantel and gamma interferon for 8 weeks.Results The area percent of collagen type Ⅰ at the 28th week after infection (40 14?17 00) increased about seven fold compared with the 8th week group (5 73?3 40). The area percent of collagen type Ⅲ at the 28th week after infection (6 80?5 19) increased about six fold compared with the 8th week group (1 15?1 34). The ? SMA positive cells also increased significantly. After gamma interferon treatment, the area percent of collagen type Ⅰ and type Ⅲ decreased significantly, from 18 51?7 52 and 4 63?3 64 (before treatment) to 3 09?1 54 and 0 40?0 37 (0 and 4 weeks after treatment) ( P

Variants of gene loci on susceptibility genes are the major individual susceptibility factors of esophageal squamous cell carcinoma (ESCC) in China.Some of the gene loci participate in the DNA damage and repair,and some are related with cancer suppressor genes,metabolism enzymes,trace elements and smoking.The single nucleotide polymorphisms of these susceptibility genes are closely correlated with the genesis of ESCC.

The external treatmen of traditional Chinese medicine, the enema and the acupuncture is the current methods of external treatment of traditional Chinese medicine treatment for cirrhosis ascites. In the field of external application and enema, most literature reported specific herbal formular for specific disease, while little literature reported combination of oral administration of herb and external application, or external application based on TCM pattern differentiation, or external application on the acupoints. Acupuncture can reduce drug use and avoid their damage on the liver. This paper summarized the current researches of Chinese medicine external treatment, and points out the direction of the research on the external treatment of traditional Chinese medicine.

Objective To analyze the changes in platelet parameters and their influential factors in cirrhotic patients with hepatocellular car-cinoma (HCC).Methods The clinical data of 602 cirrhotic patients with HCC who were admitted to the First Hospital of Jilin University from January 201 1 to December 2012,as well as 200 cirrhotic patients hospitalized during the same period,were collected.Statistical analy-sis was performed using SPSS 19.0.Normally distributed continuous data were expressed as mean ± standard deviation;comparison be-tween two groups was made by t test,and comparison between multiple groups was made by analysis of variance.Non-normally distributed data were expressed as median and interquartile range (P25 -P75 );comparison between groups was made by rank sum test.Results Com-pared with the cirrhotic group,the HCC group had significantly higher platelet count (PLT)and plateletcrit (PCT)(t=5.019,P=0.000;t=5.017,P=0.000)and a significantly lower mean platelet volume (MPV)/PLT (t=5.877,P=0.000);there were no significant differences in MPV and platelet distribution width between the two groups (t=-0.942,P=0.347;t=-1.040,P=0.298).The receiv-er operating characteristic (ROC)analysis showed that the area under the ROC curve was 0.636 for PLT,0.633 for PCT,and 0.639 for MPV/PLT in the diagnosis of HCC in cirrhotic patients.Decreases in PLT and PCT were closely related to hepatitis C virus (HCV)infec-tion.Patients with Child-Pugh class A cirrhosis had significantly higher PLT and PCT than those with Child-Pugh class B and C cirrhosis (P<0.01);patients with a maximum tumor diameter of≥5 cm had significantly higher PLT and PCT than those with maximum tumor di-ameters of2-5 cm and≤2 cm (P<0.01).Patients with Child-Pugh class A cirrhosis had a significantly lower MPV/PLT than those with Child-Pugh class B and C cirrhosis (P<0.01);patients with a maximum tumor diameter of≥5 cm had a significantly lower MPV/PLT than those with maximum tumor diameters of2-5 cm and≤2 cm (P<0.01).Conclusion PLT,PCT,and MPV/PLT can be used in the auxiliary diagnosis of HCC in cirrhotic patients,which are related to HCV,Child-Pugh classification,and tumor size.