581 cases of cholelithiasis were assigned to Western medicine group. Chinese herb group and Combined treatment group. The Western medicine group received oral administration of Trepihutorie, Ai Hou Dan Tong Tablet; The Chinese herb group were orally administrated self - formulated prescription Si Jin Pai Shi Decoction and Gan Dan Jie Shi Tablet; The combined treatment group were orally administrated Si Jin Pai Shi Decoction and received treatment with Tui An Yun Jing Instrument. Results showed that the therapeutic effect in the combined treatment group was the best, the all - stone ejection rate, total lithecbole rate and the total effective rate being ]6. 8'i%i, 49. 27%. 84. 82%, respectively; in the Chinese herb group was next, the three indexes being 11. 29%. 37. 10% and 67. 7%, respectively; in the Western medicine group was the worst, the three indexes being 2. 44%, 7. 32% and 43. 90%. respectively. There were significantly differences in the all stone ejection rate (P

Objective To investigate the effect of isoflurane preconditioning on Bcl-2 and Bax mRNA expression and the signaling pathway of nitric oxide against brain ischemia-reperfusion(I/R).Methods Seventy-five male gerbils were randomly divided into 5 groups(n=15):SHAM,I/R,ISO,AG+ISO and AG group.Global cerebral I/R was produced by occlusion of bilateral common carotid arteries for 5 minutes and confirmed by isoelectric potential on EEG.In ISO group,the animals inhaled 1.2%-1.5% isoflurane for 30 minutes followed by 30 minutes wash-out period before I/R.In AG+ISO group,30 minutes after aminoguanidine,200 mg/kg i.p.isoflurane was inhaled as ISO group,and aminoguanidine was injected 30 min before I/R in AG group.After 24 hours,the forebrains were collected,and the expression of Bcl-2 and Bax mRNA was determined by reverse transcriptase polymerase chain reaction.Results Bcl-2 mRNA expression in ISO group(1.22±0.12) was significantly higher than that in sham(0.83±0.16),I/R(0.83±0.11) and AG+ISO group(0.82±0.12)(P<0.05).Bax mRNA expression in I/R and AG group was significantly higher than that in sham group(P<0.05),and the expression in ISO group was higher than that in AG+ISO group.Conclusion Nitric oxide is involved in the signaling pathway of isoflurane preconditioning and has the protection effect on gerbil brain against I/R.

Population pharmacokinetics(PPK),which is based on the principle of statistics,can be used to estimate various inter- and intra-individual factors which affect pharmacokinetics.By using Bayesian estimator,PPK can predict individual pharmacokinetic parameters accurately.PPK is widely used in the immunosuppressive therapy with cyclosporine,tacrolimus,mycophenolic acid and sirolimus.

Objective To compare the influences between bronchial blocker (BB) and double lumen tube (DLT) in respiratory mechanics and blood gas in one lung ventilation. Methods Thirty-six patients undergoing pulmonary lobectomy or radical operation of esophageal carcinoma were randomized into group BB (17 cases) and group DLT (19 cases). In group BB,BB was intubated under the guidance of a fibreoptic bronchoscope after intubation of single lumen tube; while in group DLT, the position of the tube was confirmed using fibreoptic bronchoscopy after intubation. The peek airway pressure (Pmax) and pulmonary dynamic compliance (CLdyn) during double lung ventilation and one lung ventilation were recorded in recumbent position. Blood samples were taken from, radial artery 20 min after one lung ventilation for blood gas analysis,partial pressure of oxygen in artery(PaO2), partial pressure of carbon dioxide in artery(PaCO2), saturation of arterial blood oxygen (SaO2) were recorded. Results No significant difference was found in Pmax and CLdyn between two groups during double lung ventilation,but Pmax was significantly lower in group BB [(21.00 ± 3.59) cm H2O,1 cm H2O = 0.098 kPa] than that in group DLT [(26.89 ±4.21) cm H2O] (P＜0.05), and CLdyn was greater in group BB [(28.10 ±6.24) ml/cm H2O] than that in group DLT [(24.00±7.13) ml/cm H2O ] (P ＜ 0.05); there were no significant differences in PaO2, PaCO2, SaO2 betweentwo groups during one lung ventilation (P＞ 0.05). Conclusion The influence to respiratory mechanics in one lung ventilation is smaller using BB than using DLT, BB has blocker well applicable value in clinics.

Cancer is frequently considered to be a disease of cell cycle. Recently, it is reported that the transfer of functional ceil cycle regulators into caneer cells via vectors has the potential to intervene tumorigenesis development. This therapeutic strategy might allow cancer cells to regain missing or dysfunctional gene expression due to inhibiting aberrant proliferation or restoring phenotypes defects. As an important negative effecter of cell cycle progression, p21cip1 not only plays central roles in cell proliferation,differentiation, senescence and apoptosis, but also associates with tumor regression. In this review, we will mainly focus on discussing some recent progress in novel strategies for cancer biological therapy, related with the functions of p21cip1 in cell proliferation and apoptosis during tumorigenesis.

Objective To explore the effect of chlamydia pneumoniae(CP)infection on T lymphocyte subsets in patients with chronic obstructive pulmonary disease(COPD).Methods A total of 176 patients with acute exacerbations of COPD who were admitted to hospital from Jan.2001 to Jan.2005,and 46 healthy subjects were selected.Chlamydia pneumoniae-specific serum IgG,IgA,IgM antibodies were measured by microimmunofluorescence(MIF)test;the flow cytometer was used to analyze the frequency of CD+_3,CD+_4 and CD+_8 lymphocytes in patients with COPD and controls.Results The rate of acute chlamydia pneumoniae infection in COPD patients with acute exacerbations was 27.3% and that of chronic CP infection was 19.3%.They were all significantly higher than that in healthy subjects(P﹤0.01).The frequency of the CD+_3 and CD+_4 lymphocytes showed no difference in each group.The frequency of CD+_8 lymphocyte in COPD patients of chronic CP infection rose and the CD+_4/CD+_8 ratio was decreased in comparison with controls.On the other hand,the CD+_8 lymphocytes and the CD+_4/CD+_8 ratio showed no difference between chronic CP infection group,no CP infection group and controls.Conclusion The chlamydia pneumoniae infection is a rather frequent event in acute exacerbations of COPD,and it is an important reason to cause the disorder of cell-mediated immunity in COPD patients.The disorder of cell-mediated immunity may be involved in pathogenesis of COPD.

Objective To study content of interleuin-2 receptor(SIL-2R) pathologic relation with inflammation and fibrosis degree in patient with hepatitis of chronic B type.Methods 250 cases in hepatitis of chronic B type and 40 cases in fat liver were alive examined,according to inflammation degree different divided in to the G_1 80 example,G_2 75 example,G_3 50 example,G_4 45 example;According to fibrosis degree different S_0 40 example,S_1 60 example,S_2 50 example,S_3 50 example with the S_4 50 example;40 example fat liver of control group;The comtents of sIL-2R,HA,Ⅳ?C,PLD,LN,PCⅢ had been detected by radioimmunoassy(RIA) and enzyme immunity.Results The contents of SIL-2R of S_1,S_2,S_3 were obviously higher than that S_0(P

Objective To explore the effect of progressive active respiratory exercise on the pulmonary function and quality of life of middle-aged and elderly patients after surgery for carcinoma of the esophagus.Methods Forty post-operative middle-aged and elderly patients with carcinoma of esophagus were randomized into 2 groups.The patients in the control group were given conventional thoracic nursing,while those in the experimental group were given 3-step thoracic or abdominal breathing training,breath holding training and respiratory gymnastics training in addition.A lung function test,the 6-min walk test and a 36-item short form health survey (SF-36)were used to evaluate the subjects' pulmonary function,motor ability and quality of life after 3,6 and 12 months of treatment.Results Vital capacity (VC) increased 23％ after training.Forced vital capacity (FVC) increased 16％.Maximal ventilation volume (MVV) increased 15％ and 50％ of forced expiratory flow (FEFS0) and the diffusing capacity for carbon monoxide of the lungs (DLCO) increased 30％.Average distance in the 6-minute walk test (6MWD) lengthened from 320 m to 449 m,and the quality of life of the patients in the experimental group improved much more than that of the control group.The average pulmonary function and 6MWD of the control group did not improve significantly with only nursing care.Their average SF-36 scores for physical function,mental health,physical pain,mental role and comprehensive health did,however,improve significantly after treatment.The changes in their role limitation,social function and energy scores were not significant.Conclusion Early and progressive active respiratory exercise can ameliorate lung dysfunction after a thoracic operation,and improve motor ability and quality of life.

Objective:To introduce the local human body metabolic rate measurement system based on the Smartphone with the Android operating system and realizes the transmission of physiological data from the detecting module to Smartphone. Methods:Embedding Bluetooth module in the module of detecting physiological parameters, the measurement target is fingers, so the detected physiological data can be sent to Smartphone with Android system. By develop apk application program, the Smartphone can realize the operation of opening the Bluetooth devices and searching the around Bluetooth devices, to realize the data transmission by the Socket connection, we develop the software which realizes showing data. Results:The Smartphone received the metabolic rate parameters from Bluetooth, and show the result on the surface. A study of the technology of transmitting physiological parameters information through the interface of Bluetooth in Android Smartphone is made in this paper. Conclusion:The paper is based on the Mobile Medicine Measuring System, realizes the technology of physiological parameters transmission through Bluetooth in Android system, which has a great broad application.

Objective To analyze the relationship between genotype and phenotype of vitelline macular dystrophia (VMD2) gene in a family with Best disease, and to provide the theoretical basis for gene diagnosis of Best disease. Methods Mutation in the coding regions and the promotor sequence of VMD2 gene from 10 members in a family with Best disease were screened by polymerase chain reaction (PCR) and direct DNA sequencing, and combined with a conformation sensitive gel electrophoresis (CSGE) approach, VMD2 gene screening was performed on 100 normal control individuals. Results In the 10 members, T→C nucleotide change at the 223 base of exon 3 was detected in 9, including 6 with Best disease who was confirmed by ophthalmoscopy and electrophysiological examination in whom 2 were affirmed as having homozygote of this mutation. Other 3 young family members with VMD2 gene mutation only had abnormal electro-oculogram manifestations. Above mutation was not detected in the normal control individuals. Conclusions The phenotype and genotype of VMD2 in the family with Best disease is highly correlated. Mutation in VMD2 gene is the nosogenesis in this family. Mutation screening of VMD2 gene can be used for genic diagnosis and genetic consultation of Best disease.