Objective observe the post-radiation expression of rat mesangial cell plasminogen activator inhibitor-1 (PAI-1), and the effect of PPAR-? ligand on radiation induced PAI-1 expression. Methods After 10?Gy ?-ray radiation of cultured rat kidney mesangial cell, RT-PCR was used to observe PPAR-?expression. PAI-1 expression after 10?Gy radiation ?10?mmol/L pioglitazone (one of PPAR-? ligands) was measured by Northern blot and Western blot. Gel-shift method was used to study the effect of AP-1 of PPAR-? ligand on radiation induced PAI-1 expression. Methods Rat kidney mesangial cell expressed PPAR--? mRNA. PAI-1 over-expressed at 24 hour after 10?Gy radiation in a radiation dose dependent manner, but not at other time. When the cell was treated with 10?Gy + 10?mmol/L pioglitazone, PAI-1 upregulation was blunted at message and protein level. AP-1 activation was increased from the 2nd hour after 10?Gy radiation and reached the highest level at the 4th hour. When pioglitazone was added, the radiation induced increase in AP-1 activation was suppressed at the 4th hour. Conclusions Pioglitazone, mediated by AP-1 gene, can suppress radiation induced PAI-1 upregulation.

Objective This study sought to determine the normal ranges of N-terminal pro-brain natriuretic peptide (NT-proBNP) in normal children and children with symptoms of heart failure (HF) caused by dilated cardiomyopathy (DCM),and further study their clinical implications.Methods Concentrations of Serum NT-proBNP were determined in 80 normal children and 45 children with clinical symptoms of HF.Venous blood was taken in each subject,and plasma NT-pro BNP was determined by ELISA method.Of the 80 normal children,40 were boys and 40 were girls.Their age ranged from 1 to 16 years old with a mean of 10.6?4.2 years.The 45 children with HF ,caused by dilated cardiomyopathy (DCM).Their age ranged from 1 to 16 years,and had a left ventricular ejection fraction (LVEF) of less than 50%.The serum NT-proBNP levels were correlated to LVEF measured by echocardiography and clinical symptom score of heart failure defined by using Ross Score.Results The mean (?s) serum concentration of NT-proBNP was 223.1?76.6fmol/ml in normal children from 1 to 16 years old.NT-proBNP levels did not show a significant age-related or sex-related differences.In children with HF,the plasma NT-proBNP levels were significantly elevated (mean:1353.3 fmol/ml range: 341.6 fmol/ml～2794.1 fmol/ml) as compared to normal children.(P

Supported by Center of Laboratory Medicine of PLA,the Third Military Medical University launched teaching reform on laboratory diagnostics by integrating and optimizing teaching contents,adopting case centered teaching method,introducing thinking modes of evidence-based laboratory medicine and strengthening bilingual teaching.Abilities of students to determine the clinical stage and to evaluate the effect of treatment as well as prognosis of diseases were promoted.

OBJECTIVE To explore the changes in foot fungus mobility and its drug resistance for further etiology investigation and clinical treatment. METHODS Sabourand′s agar culture medium was used to culture fungi, ID identification strip was employed to identify the fungi and drug sensitive test was performed by disk diffusion test. RESULTS The incidence of Trichophyton rubrum infection was the highest (79.1%). The isolated fungi were relatively sensitive to amphotericin B (AMB, 98.9%) and itraconazole (ITC, 98.0%), and resistant to 5-fluorocytosine (5-FC, 22.1%). CONCLUSIONS Detection technique of fungal infection should be improved and anti-fungal medicine should be used reasonably according to the results of drug sensitive test so that the fungal infection, especially fungi-resistant infection could be reduced.

OBJECTIVE To monitor the characteristics of distribution and drug resistance of Acinetobacter baumannii in our hospital. METHODS A. baumannii isolates were collected in our hospital from Jan 2004 to Dec 2005. Antimicrobial susceptibility testing was performed by disk-diffusion method,according to the standards of NCCLS 2004. RESULTS Totally 177 strains of A. baumannii were distributed clinically in the respiratory unit as the most ones (47 strains, 26.6%), and in ICU as the next (38 strains, 21.5%); the older the age, the higher the appearing rate; the highest appearing rate was from the sputum, up to 78.1%; more than 60% of isolates were resistant to all antimicrobial agents tested except imipenem, meropenem and cefoperazone/sulbactam. However,10 pan-resistant strains were found. CONCLUSIONS With the increasing isolation rate of A. baumannii, its drug resistance increases simultaneously.

Objective To map the complete methylation status of the hMLH1 promoter in sporadic colorectal carcinoma and analyze the relationship between MVPs (methylation variable positions) of hMLH1 promoter and the expression of hMLH1. Methods Methylation status of hMLH1 promoter was measured by bisulfite sequencing. hMLH1 protein expression was detected by immunohistochemistry. Results Out of the 30 sporadic colorectal carcinoma specimens, the hypermethylation of CGIs (CpG islands) 1 was in 6 and that of CGIs 2 in 4. The hMLH1 protein was detected in 15 specimens. Chi square test showed the methylation of CGIs 1 was closely related to loss of hMLH1 protein expression (P0.05). Conclusion In CGIs 1, CpG positions from 1 to 28 are the critical region that could influence the expression of hMLH1.

OBJECTIVE To investigate the methylation status and mRNA expression of the estrogen receptors(ERs) gene promoter in acute leukemic patients and detect the protein expression in leukemia cell lines with or without treatment of 5'-aza-Dc.And to find out the possibility of promoters methylation profile of estrogen receptor gene as an early diagnosis biomarker in leukemia.METHODS With RT-PCR and MSP,evaluating ERs mRNA expression and status of methylation in 40 acute leukemia patients without treatment.With Western-blot,detecting protein expression in leukemic cell lines with or without treatment of 5-azaDc.RESULTS The protein expression was significantly enhanced in all of leukemic cell lines with 5'-Aza-Dc.ER?-A was inactivated and specifically methylated(97.5%;39/40) in most of the acute leukemic patients.CONCLUSIONS The promoter ER?-A is inactivated and specifically methylated(97.5%;39/40) in most of the acute leukemic patients.This study may provide a new direction method to study the pathogenic mechanism of leukemia,and indicates that ER?-A methylation could be a potential reference marker for leukemia diagnosis.

OBJECTIVE To investigate the epidemiology of fungemia and provide evidence for clinical therapy.METHODS A retrospective survey was done with the 69 cases of fungemia in our hospital from Jan 2004 to Dec 2008.RESULTS More than 65% of the patients suffered from two and more underlying diseases.Over a half of infections were developed following placement of catheters.And all of the patients had a history of antimicrobial agents use before blood culture.53(76.8%) cases were associated with Candida spp.Only 18 strains were C.albicans.The mortality rate of fungemia was 44.9%.Different Candida species had different resistance rates to antifungal agents.CONCLUSIONS Fungemia patients often have serious underlying diseases.Most fungemia cases are candidemia caused by non-C.albicans.Some fungal pathogens are resistant to fluconazole and itraconazole.

Objective To investigate the variation of vascular cell adhesion molecule-1 (VCAM-1) expression and lipid peroxidation in aorta and plasma of Ins2~ Akita diabetic mice, and to inquire into the relationship between the variation and the cardiovascular diseases in diabetic patients. Methods With the aortic and plasma samples from Ins2Akita diabetic mice, the protein expression levels of VCAM-1 were detected by Western Blot or ELISA, and the levels of lipid peroxides were determined by lipid peroxidation kit. Results Compared with that of normal control, VCAM-1 protein and lipid peroxides levels were significantly elevated in the aorta and plasma of Ins2~ Akita diabetic mice (P

OBJECTIVE To analyze pathogenic bacterium distribution and antibiotic resistance of our hospital,and provide scientific basis for clinical rational using of antibiotics.METHODS The patients′ clean catch(midstream)(urine) was collected from Jan 2004 to Dec 2005 and cultivated.Antibiotic sensitivity test and adopted by Kirby-Bauer method.RESULTS The pathogenic bacteria mainly consisted of Gram-negatives,among which Escherichia coli was the most frequent,the others in turn were Pseudomonas aeruginosa,Enterobacter cloacae and(Proteus) mirabilis;Enterococcus were the most common among Gram-positives;fungal infection obviously(increased).The bacteria showed different antibiotic resistance rate and multi-drug resistance.CONCLUSIONS It′s very important for making the clinical use of antibiotic more reasonable and controlling drug resistant strains(transmission).