Psoriasis is a recrudescent chronic inflammatory dermatosis which is called“tinea”in ancient times. Its pathogenesis is not only associated with changes in the blood and body fluids, but also has close connection with external infection of six evil factors. This article sorted data of pathogenesis and treatment from the perspective of external infection of six evil factors, and discovered that pathogenesis of psoriasis includes external factors of four evils of wind, cold, damp and heat and internal factors of blood deficiency and blood dryness. Ancient treatment was based on dispelling wind, clearing away heat and moistening dryness. The pathogenesis can be summarized as follows:wind and poison attack skin and hinder blood production. The treatment can be “let in air for detoxification, enrich blood and moister dryness, cooling and activating blood”, blood and functional status of organs of patients should be observed, with a purpose to provide references and basis for modern clinical prevention and treatment of psoriasis.

70%). Imipenem was of the strongest activity against Pseudomonas aeruginosa with the susceptibility of 86.3% but cefetaxime and ceftriaxone were of the weakest with the susceptibility of 6.8% and 11.0%, respectively. Conclusion The clinically isolated drug resistant Pseudomonas aeruginosa strains are increasing year by year. Therefore, clinical doctors should choose antibiotics rationally in dealing with the P. aeruginosa infected patients according to drug resistance test.

neoplasia.

Supported by Center of Laboratory Medicine of PLA,the Third Military Medical University launched teaching reform on laboratory diagnostics by integrating and optimizing teaching contents,adopting case centered teaching method,introducing thinking modes of evidence-based laboratory medicine and strengthening bilingual teaching.Abilities of students to determine the clinical stage and to evaluate the effect of treatment as well as prognosis of diseases were promoted.

The terahertz laboratory medicine (THz-LabMed) is an intergrated cross-frontier field which involves multi-disciplinary including medicine,biology,biomedical engineering,physics,optics,computer science,information and materials.Using THz technologies for label-free detection and analysis of biomedical macromolecules,cell and tissues,the THz-LabMed is also the core components of terahertz biomedine (THz-BioMed) which focuses on the biomedical application of THz technologies.THz label-free detection is being paid more and more attention because of its unique advantages worldwide and becomes a hot spot for the application of THz wave technology and methods in life science.Application of THz technology in biomedicine involves many fields globally,including disease diagnosis,recognition of protein status,label-free DNA sequencing,mechanism for absorption differences of biological tissue to THz wave,and radiation influence on biological samples and biological process.THz-LabMed is the global synchronized research in THz-BioMed field in China.It is important to seize the opportunities to develop new disciplines of laboratory medicine.

Objective To elucidate the role of human bone marrow stromal cells (hBMSC) in combination with exogenous cytokines such as stem cell factor(SCF) and FLT 3 ligand (FL) in supporting the in vitro expansion of CD 34 + cells purified from cord blood.Methods CD 34 + cells were isolated from umbilical cord blood by using a high gradient magnetic cell sorting system(MACS),expanded in combination with SCF,IL3 (interleukin 3),IL 6 (interleukin 6),FL,EPO (erythropoietin),and were planted onto the pre established irradiated (20Gy) stroma layer(hBMSC plus combinations of cytokines) respectively.On day 10,total cells were counted, and hematopoietic progenitor cells were assessed by semisolid culture assay,CD 34 + cells were quantitated by FACS.Results After separation by MACS,the frequency of CD 34 + cells was 92%?0.04%.On day 2,almost all the inoculated cells adhered to the stroma layer,with only a small number in the supernatant.Then,cells in the supernatant increased gradually, but the percentage of CD 34 + cells decreased.Compared with control, expansion fold of CD 34 + cells, CFU GM, BFU E were significantly higher in hBMSC group (P 0.5).Conclusion ① CD 34 + cells from cord blood formed foci adherent to the monolayer and colony forming cells remained in the culture of 10days,indicating that hBMSC can support hematopoiesis in vitro;②using human bone marrow stromal cells in cooperation with exogenous cytokines may be a feasible way to expand hematopoietic stem/progenitor cells.

Objective To set up a calibration system for automated hematology analyzers with fresh blood in clinical laboratory.Methods Fresh blood assigned by a traceable measurement system was used to calibrate nine hematology analyzers, and compared the bias before and after calibration.Results In the parameters to be calibrated for the hematology analyzers, there was about 55.6% (25/45) over allowable bias before calibration but 15.6% (7/45) after calibration with fresh blood. Among the results of bias over allowable upper limit were mostly existed in 3-part differential hematology analyzers, and mainly focused on WBC and PLT.Conclusion It is available to calibrate different hematology analyzers with fresh blood in a clinical laboratory.

Objective To map the complete methylation status of the hMLH1 promoter in sporadic colorectal carcinoma and analyze the relationship between MVPs (methylation variable positions) of hMLH1 promoter and the expression of hMLH1. Methods Methylation status of hMLH1 promoter was measured by bisulfite sequencing. hMLH1 protein expression was detected by immunohistochemistry. Results Out of the 30 sporadic colorectal carcinoma specimens, the hypermethylation of CGIs (CpG islands) 1 was in 6 and that of CGIs 2 in 4. The hMLH1 protein was detected in 15 specimens. Chi square test showed the methylation of CGIs 1 was closely related to loss of hMLH1 protein expression (P0.05). Conclusion In CGIs 1, CpG positions from 1 to 28 are the critical region that could influence the expression of hMLH1.

Objective To investigate the variation of vascular cell adhesion molecule-1 (VCAM-1) expression and lipid peroxidation in aorta and plasma of Ins2~ Akita diabetic mice, and to inquire into the relationship between the variation and the cardiovascular diseases in diabetic patients. Methods With the aortic and plasma samples from Ins2Akita diabetic mice, the protein expression levels of VCAM-1 were detected by Western Blot or ELISA, and the levels of lipid peroxides were determined by lipid peroxidation kit. Results Compared with that of normal control, VCAM-1 protein and lipid peroxides levels were significantly elevated in the aorta and plasma of Ins2~ Akita diabetic mice (P

Objective To design a system for the detection of coagulation factors using piezoelectric quartz crystal (PQC) sensors and to introduce its operating principle and the main components. Methods The contrary piezoelectric response of quartz crystal was used. Based on the fuzzy and decoupling control, a singlechip computer was used for constant temperature and constant humidity control and frequency count. The plasma coagulation time was measured for the purpose of determination of the activities and contents of the coagulation factors. Results The system was convenient, precise, and consistent for the detection of the coagulation factors. Conclusion PQC sensors with high accuracy and low cost can be used in the detection of coagulation factors and has a bright future in clinical practice.