The relative content of DNA-RNA and the nuclear area/nucleolar area ratio of the hyperplastic tissue,glottic polypus,dysplastic tissue of grade I to I laryngeal squamous cell carcinoma were measured in 33 cases with image analysis technique.It was found that the changes of DNA-RNA content and the ratio of nuclear/nucleolar area were parallel to those of the severity of the carcinoma,and RNA was markedly increased in grade 11 and I dysplastic tissue.On the basis of the current grading of laryngeal pathology,the combined determination of DNA-RNA can provide more sensitive indicators to the stages of malignant degeneration of the laryngeal squamous cell carcinoma.

Biliary complications are a serious problem in patients after liver transplantation, which often results in graft loss, the causes of bile duct stricture are complex. This paper reviewed the mechanism of bile duet stricture after liver transplantation. This complication maybe related with various factors such as liver arterial thrombosis, loss of blood supply of bile duct, warm iscbemia/preservation injury, chronic ductopenic rejection, cytomegalovirus infection, ABO incompatibility, and recurrence of primary sclerosing cholangitis.

Objective To investigate the effect of total sleep deprivation (TSD) shown in the electroencephalogram (EEG) power spectra in healthy young men. Method 20 healthy young male volunteers (19～22 years old) were subjected to 50 hours of TSD. EEGs were recorded 8 times (6 hours intervals) during TSD. EEG power was calculated for six bands at all 16 derivations.Results 50 hour TSD induced an increase in ? power at the other derivations except both occipitals ( P

Objective To evaluate the usefulness of the submucosal tunnel ureteroileostomy using ileovalvular segment as afferent loop in preventing ureteral refluxes and upper urinary tract infection in detenia ceacal-colon continent urinary diversion. Methods Ten patients underwent submucosal tunnel ureteroileostomy in detenia ceacal-colon continent reservior and detenia coloneobladder and the follow up for ureteral reflux and upper urinary tract infection was carried out from 1998 to 2001. Results The patients were followed up for 6 to 36 months.No evidence of ureteral reflux and upper urinary tract infection was observed.Unilateral hydronephrosis was found in one patient caused by the ureteroileal anastomotic stricture. Conclusions Submucosal tunnel ureteroileostomy is an effective fashion for detenia ceacal-colon continent urinary diversion in preventing the postoperative complications of ureteral reflux and upper urinary tract infection.

Objective To observe clinical effect of treating ophthalmoplegia with acupuncture combined with point injection. Methods 25 cases patients with ophthalmoplegia were randomly recurited into a control group and a treatment group. The control group was treated with western medicine and the treatment group was treated with acupuncture combined with point injection. The clinical effect of both groups was observed after treatment. Results The clinical effect in the treatment group was significantly higher than that of the control group (P<0.05) . Conclusion The treatment of ophthalmoplegia with acupuncture combined with point injection has a good effect.

Objective To observe clinical effect of treating blepharospasm with acupuncture. Methods 27 patients with blepharospasm were randomly recruited into a control group and a treatment group. The control group was treated with western medicine, and the treatment group was treated with acupuncture. The clinical effects of both groups were observed. Results Total effective rate in the treatment group and the control group was 85.7% and 69.2%, respectively. There was a significant dieffference between the two groups (P<0.05) . Conclusion The treatment of acupuncture on blepharospasm is better than western medicine.

Objective To analyze the long-term results of fractionated stereotactie radiotherapy(FSRT)for the local residual lesion after the first course of radiotherapy for nasopharyngeal carcinoma.Methods From July 1997 to July 2002,46 patients were treated with FSRT.According to the 1992 Fuzhou staging system,the number of patients was 1,6,30 and 9 with stage Ⅰ,Ⅱ,Ⅲ and Ⅳ disease,respectirely;3,11,27 and 5 with T1,T2,T3 and T4 tumor,respectively;14,16,12 and 4 with N0,N1,N2and N3 disease.Radiotherapy was delivered to tumors with the total of dose 68-70 Gy in 7-8w.Chemotherapy(2 cycles of PVF or POF)was given to the patients with stage Ⅲ and Ⅳ a disease.FSRT was given to the residual disease with the total dose of 18-24 Gy in 3 fractions with an interval of 3-7 days.The reference dose line was 70%-90%.Resuits CR and PR rates in this group were 61%and 39%,respectively.The overall survival rates of each year from 1- to 5-year were 100%,87%,83%,78%and 76%.The 1-,3- and 5-year disease-free survival rates were 100%,93%and 89%;The distant metastasis-free survival rates were 100%,85%and 79%;The local-regional control survival rates were 100%,94%and 91%.Seventeen patients who died during the follow-up period were 1 for local cervical lymph node recurrence,2 for fatal nasopharyngeal hemorrhage,4 for local nasopharynx recurrence,and 10 for distance metastases. Conclusions Fraetionated stereotactic radiotherapy is safe and effective for the patients with residual lesion of nasopharyngeal carcinoma at the primary site after radiotherapy.The optimized fractionation and total dose requires the further investigation.

Objective To investigate the correlation of left ventricular ejection fraction (LVEF) as well as serum levels of NT-proBNP, Hcy and D-Dimer (D-D) with different traditional Chinese medicine (TCM) syndrome types of chronic heart failure (CHF). Methods A total of 178 CHF patients were divided into heart function normal ejection fracture group (HF?NEF, n=86) and heart function reduction (HFREF, n=92) according to their LVEF performance. Another 35 cases with nor?mal cardiac function were included in control group. All CHF patients was also divided into 3 TCM syndrome types:both de?ficiency of Qi and Yin syndrome group(n=64),Qi asthenia causing blood stasis syndrome group(n=59) andYang defi?ciency water stop group (n=55). All patients were examined with cardiac color doppler and LVEF values were recorded. And serum NT-proBNP、Hcyand D-D levels were all quantified. Results As to serum levels of NT-proBNP, Hcy and D-D, they were higher in HFREF group than those in HFNEF group than those in control group. On the other hand, LVEF was lowest in HFREF group but highest in control group. All differences were statistically significant (P<0.05). Among patient in HFNEF group, LVEF in theYang deficiency water stop groupwas lower than that inboth deficiency of Qi and Yin syn?drome group(P<0.05). Serum levels of NT-proBNP, Hcy, and D-D were not significantly different between different TCM syndrome groups. By contrast, among patients in HFREF group, LVEF values did not differ significantly between different TCM syndrome groups. Serum level of NT-proBNP was lower inboth deficiency of Qi and Yin syndrome groupthan that inQi asthenia causing blood stasis syndrome group than that in Yang deficiency water stop group. As to serum levels of Hcy and D-D, they are higher inYang deficiency water stop groupthat those inboth deficiency of Qi and Yin syndrome group and Qi asthenia causing blood stasis syndrome group(P<0.05). Conclusion Patients with different TCM syn?drome types of CHF present different levels of serum NT-proBNP, Hcy, D-D level and LVEF. Changes of indicators in HFREF groups are more obvious than they did in HFNEF group.

AIM:To explore the inhibitory action of recombinant human tumor necrosis factor-related apoptosis-inducing ligand(rhTRAIL) on mouse breast cancer. METHODS:Each mouse was inoculated 0.2 mL (1?106) D2F2 cells subcutaneously in the right lower limb and they were divided into five groups randomly. The control group was infused PBS 0.2 mL,while the low-dose,medium,high groups received purified rhTRAIL 2.5 mg/kg,5.0 mg/kg,10.0 mg/kg,respectively,the positive group was administered cyclophosphamide 30.0 mg/kg. Every group was operated by peritoneal injection once a day for fifteen days. The mice were weighed every day. The growth state was viewed and the size of the tumor was measured every 3 d to calculate the tumor volume and tumor suppression rate. All mice were killed after 15 d. The pathologic changes of the tumor were observed under light-microscopy and electronic microscopy. The cell cycle and apoptosis index of D2F2 cells were analyzed by flow cytometry. RESULTS:The body weight and tumor volume in low-dose,medium,high groups were lower than those in control group and the restriction effect was more significant than that in the control group (P

Porcine transfer factor(TF-P) was prepared from porcine spleen by means of hom-ogenization and vaccum dialysis. Fractionized by Sephadex G-10 gel chromatography,TF-P could be separated into seven fractions but five fractions were obtained from human transfer factor(TF-h) .These fractions of TF-P and TF-h and different dilutionsof TF-P were tested for biological activity in vitro. By the use of ~3H-TdR uptake assay we found that, both of whole TF-P and TF-hpossessed suppresivs activity to PHA-induced human periperal blood lymphocyte tran-sformation. Whereas, Fraction Ⅲ of TF-P and Fraction Ⅲ and Ⅳ of TF-h containedcomponents responsible for augmentation of PHA-induced responses. When TF-P was diluted 10~(-2) to 10~(-5) and cultured with normal human peripheralblood lymphocytes, we found that the original suppression effects of whole TF-P toPHA-induced lymphocyte transformation were converted to normal. The optimum dil-ution of TF-P was 10~(-3), which were shown to augment responses. It shoud be notedthat the responsiveness of normal human lymphocytes to TF-P was dose dependent.