1.Study on the development of an evaluation index system for electricity saving at general hospitals
Honglin LI ; Xin ZHANG ; Shixin WANG ; Weike CHEN ; Yue LI ; Ying HAN
Chinese Journal of Hospital Administration 2017;33(7):537-539
Objective To establish an evaluation index system of electricity saving at general hospitals.Methods Based on civil building energy saving studies and in accordance with national regulations on hospital energy saving, the authors build an electricity saving index system for general hospitals.The indexes were reduced by the rough set theory, and their weight was determined by analytical hierarchy process and expert analysis.Results An electricity saving evaluation index system for general hospitals is so developed, consisting of six level-2 indexes and 27 level-3 indexes.Conclusions Such an evaluation index system can guide hospital electricity consumption and saving.
2.Chronic conditions of type 2 diabetes in a community:262 case analyses
Jie DU ; Liangpu PENG ; Yuhua ZUO ; Suying JIANG ; Weike XIN ; Wen XU ; Lanhua LIU ; Naizhen ZHEN ; Rong FANG ; Xuhong HOU ; Weiping JIA
Chinese Journal of General Practitioners 2008;7(5):336-337
This study was to assess the chronic morbidity and metabolic disordelters in 262 patients with type 2 diabetes.Of all participants,64(24.4%)coexisted with peripheral neuropathy,34(13.0%)combined with peripheral vascular disease.41(15.6%)were diagnosed as diabetic retinopathy,and 46 (17.6%)had concurrent diabetic nephropathy.In comparison with diabetic patients without these complications,those with the chronic conditions generally had higher plasma glucose,blood pressure or body mass index.
3.Downregulation of cardiac PIASy inhibits Cx43 SUMOylation and ameliorates ventricular arrhythmias in a rat model of myocardial ischemia/reperfusion injury.
Tingting WANG ; Jinmin LIU ; Chenchen HU ; Xin WEI ; Linlin HAN ; Afang ZHU ; Rong WANG ; Zhijun CHEN ; Zhengyuan XIA ; Shanglong YAO ; Weike MAO
Chinese Medical Journal 2023;136(11):1349-1357
BACKGROUND:
Dysfunction of the gap junction channel protein connexin 43 (Cx43) contributes to myocardial ischemia/reperfusion (I/R)-induced ventricular arrhythmias. Cx43 can be regulated by small ubiquitin-like modifier (SUMO) modification. Protein inhibitor of activated STAT Y (PIASy) is an E3 SUMO ligase for its target proteins. However, whether Cx43 is a target protein of PIASy and whether Cx43 SUMOylation plays a role in I/R-induced arrhythmias are largely unknown.
METHODS:
Male Sprague-Dawley rats were infected with PIASy short hairpin ribonucleic acid (shRNA) using recombinant adeno-associated virus subtype 9 (rAAV9). Two weeks later, the rats were subjected to 45 min of left coronary artery occlusion followed by 2 h reperfusion. Electrocardiogram was recorded to assess arrhythmias. Rat ventricular tissues were collected for molecular biological measurements.
RESULTS:
Following 45 min of ischemia, QRS duration and QTc intervals statistically significantly increased, but these values decreased after transfecting PIASy shRNA. PIASy downregulation ameliorated ventricular arrhythmias induced by myocardial I/R, as evidenced by the decreased incidence of ventricular tachycardia and ventricular fibrillation, and reduced arrythmia score. In addition, myocardial I/R statistically significantly induced PIASy expression and Cx43 SUMOylation, accompanied by reduced Cx43 phosphorylation and plakophilin 2 (PKP2) expression. Moreover, PIASy downregulation remarkably reduced Cx43 SUMOylation, accompanied by increased Cx43 phosphorylation and PKP2 expression after I/R.
CONCLUSION
PIASy downregulation inhibited Cx43 SUMOylation and increased PKP2 expression, thereby improving ventricular arrhythmias in ischemic/reperfused rats heart.
Rats
;
Male
;
Animals
;
Myocardial Reperfusion Injury/metabolism*
;
Connexin 43/genetics*
;
Sumoylation
;
Down-Regulation
;
Rats, Sprague-Dawley
;
Arrhythmias, Cardiac/drug therapy*
;
Myocardial Ischemia/metabolism*
;
RNA, Small Interfering/metabolism*