0.05), but the time to initiate effect in the treated group [(14.98?9.28) days] was obviously shorter than that of control group [(30.88?11.32) days] with significant difference (P

Objective To study the impact of diabetes mellitus (DM) and postoperative blood glucose control on the clinicopathological characteristics and prognosis of patients with pancreatic cancer who underwent radical surgery.Methods From January 2010 to January 2015, the clinical and follow-up data of 83 patients with pancreatic cancer who underwent radical surgery at Tianjin Medical University General Hospital were retrospectively studied.Patients were classified into the diabetic group (28 cases) and the non-diabetic group (55 cases) according to the preoperative status of DM.The clinicopathological characteristics, postoperative conditions and prognosis were compared.Results The incidences in the two groups of patients whose postoperative blood glucose levels were poorly controlled were 71.4％ (20/28) and 20.0％ (11/55) in the diabetes group and the non-diabetic groups, respectively.The difference was significant (P ＜0.05).The 3-year survival rate of the diabetic and the non-diabetic groups were 11.3％ and 31.8％ , respectively.The difference was significant (P ＜ 0.05).The 3-year survival rate of patients with poorly-controlled blood glucose levels were 8.8％, and 34.6％, respectively.The difference was significant (P ＜ 0.05).Conclusions The postoperative blood glucose level in pancreatic cancer patients with DM was poorly controlled after radical surgery.The prognosis of pancreatic cancer patients with DM or poorly postoperative blood glucose control was poor after radical surgery.

Objective To study the effect of Radix Salviae Miltiorrhizae (RSM), a kind of Chinese Traditional Medicine which can dissolve stasis by activating blood circulation, on proliferation, invasion, adhesion, migration and metastasis of B16-BL6 metastatic mouse melanoma cells and discuss its functional mechanism. Methods The proliferation, adhesion, invasion and migration capacity of B16-BL6 metastatic cells was evaluated by MTT assay, adhesion assay and reconstituted basement membrane invasion and migration assay in vitro respectively. Mouse spontaneous melanoma model was used to study the effect of RSM on metastasis in vivo. Results The extract of RSM bidirectionally adjusted the multiplication of B16-BL6 cells, promoted prominently the adhesion of B16-BL6 to Laminin, inhibited significantly B16-BL6 invading reconstituted basement membrane and the migration of B16-BL6. In the mouse spontaneous melanoma model, it suppressed significantly the volume of lung metastatic nodes but had little effect on the number of lung metastatic nodes. Conclusion The extract of RSM can alleviate the degree of the metastasis of B16-BL6 metastatic mouse melanoma cells, which may be related with inhibiting the B16-BL6 cells invading the extracellular matrix and reducing the migration of B16-BL6 cells.

Objective To explore the clinical value of serological indicators in diagnosis of patients with different degree of liver cirrhosis.Methods 50 patients with liver cirrhosis and 50 patients with decompensate cirrhosis were selected.The control group had 50 patients.The serological indicators were statistically analyzed.Results TBIL and ALT in the decompensated liver cirrhosis group were (69.5 ± 7.0) μmol/L,(143.1 ± 14.2) U/L,which were higher than those of compensated liver cirrhosis group (44.6 ± 5.8) μmol/L,(77.4 ± 8.6) U/L (P ＜ 0.05),and those two groups were higher than the control group(P ＜ 0.05).Alb and ChE of decompensated liver cirrhosis group were (28.2 ± 3.7) g/L and (2024.39 ± 211.40) U/L,which were lower than those of the control group (36.1 ±3.7) g/L,(6169.36 ± 607.42) U/L and the compensated cirrhosis group [(34.7 ± 4.3) g/L,(3571.27 ± 310.01)U/L] (P ＜0.05).ChE of compensated cirrhosis group was lower than the control group (P ＜0.05).PA and ADA of decompensated and compensated liver cirrhosis group were (0.14 ±0.04)mg/L,(16.17 ± 1.94)U/L and (0.21 ±0.05) mg/L,(34.20 ± 3.29) U/L,the differences were statistically significant (P ＜ 0.05).A/G of decompensated and compensated liver cirrhosis group were (0.64 ± 0.29) and (1.06 ± 0.30),which were lower than the control group (1.51 ± 0.21) (P ＜ 0.05),and that of the decompensated cirrhosis group was lower than that of the compensated cirrhosis group(P ＜0.05).Ⅳ-C and LN of decompensated cirrhosis group were (97.4 ±9.8) μg/L and (205.7 ±20.1) μg/L,which were higher than those of compensated liver cirrhosis group (68.7 ± 7.5) μg/L and (124.1 ±11.8) μg/L and the control group(52.3 ±6.1) μg/L and (83.8 ±7.6) μg/L(P ＜0.05).And those of the compensated cirrhosis group were higher than the control group (P ＜ 0.05).HA and PC Ⅲ of decompensated cirrhosis group were (211.3 ± 16.4) μg/L and (168.1 ± 16.2) μg/L,which were higher than the control group (51.2 ±5.3) μg/Land (79.1 ± 8.0) μg/L (P ＜ 0.05).Conclusion The serological indicators had the important reference value for clinical diagnosis and prognosis of cirrhosis.

Objective To evaluate the expression of Yes-associated protein (YAP) in colorectal carcinoma and analyze its influence on tumor cell proliferation.Methods The expressions of YAP in 94paired colorectal carcinomas and pericancerous normal tissues were detected by using immunohistochemistry method.The expressions of YAP in colorectal carcinoma cell line HCT116 were inhibited with a YAP-spe-cific siRNA.Cell proliferation was then determined by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay.Results The positive rate of YAP in colorectal carcinomas was significantly higher than that in pericancerous normal tissues [69.1％ (65/94) vs 22.3 ％ (21/94),P ＜ 0.001].The expression of YAP was associated with tumor Node Metastasis(TNM) stage and lymph node metastasis(P ＜0.05),but not associated with gender,age,tumor location and histological grade(P ＞0.05).After YAP-specific siR-NA was transfected into HCT116 using lipofectamine,the expression of YAP mRNA and protein in the experimental group were reduced by (78.2 ±2.1)％ and (81.7 ± 1.5)％,respectively,with a statistically significant difference (t =67.55,91.601,P ＜0.01).The growth of HCT116 was significantly inhibited and the reduced rate of cell proliferation was (28.1 ± 1.6) ％,(34.7 ± 2.4) ％ and (24.7 ± 1.2) ％ at the time point of 48 h,72 h and 96 h,respectively.Conclusions Expression of YAP was upregulated in colorectal carcinomas and downregulation of YAP expression could inhibit growth of colorectal carcinoma cells.YAP can be used as a new candidate target for diagnosis and treatment of a colorectal carcinoma.

Background:Conventional gastrointestinal endoscopy is incapable of determining the deriving layers,size and nature of submucosal lesions,however,mini probe ultrasonography(MPS)is effective for mural stratification and determining the deriving layers and nature of lesions within gastrointestinal wall,and is considered to be an optimal examination for suspected submucosal tumors before endoscopic or surgical operation. Aims:To assess the diagnostic value of MPS for gastrointestinal submucosal lesions and the significance of MPS-assisted endoscopic therapy. Methods:A total of 69 patients with presumed gastrointestinal submucosal protruded lesions were retrospectively enrolled. All of them underwent MPS and then endoscopic therapy,such as cyst incision,high frequency electric snare resection,endoscopic mucosal resection and endoscopic submucosal dissection were performed according to the deriving layers,size and nature determined by MPS. The ultimate diagnosis was confirmed by histopathological examination. Results:In the 69 cases of lesions,MPS showed that 15 were derived from muscularis mucosa,40 from submucosa,and 14 from muscularis propria;10 of them were considered as cyst,18 were stromal tumor,8 were leiomyoma,6 were ectopic pancreas,15 were neuroendocrine tumor,and 12 were lipoma. Compared with pathological diagnosis,an overall coincidence rate of 91. 3%(63 / 69)was achieved by MPS. Conclusions:The accuracy rate of MPS is high for determining the deriving layers and nature of gastrointestinal submucosal protruded lesions prior to the attempting of endoscopic removal. It might be helpful for selecting treatment modalities for this kind of lesions.

Objective To improve the diagnostic accuracy of silicosis by investigating its CT appearances.Methods CT appearances in 45 patients with silicosis were analyzed retrospectively.All these patients were male,aged from 42~71 years(mean age,56 years old),including stageⅠin 5,stageⅡin 18 and stage Ⅲ in 22 cases.All patients underwent plain CT scan,CT findings of lung parenchyma,hilus, mediastinum,and pleura were analysed.Results CT appearances of silicosis included:(1)Lung parenchyma:①All cases showed diffuse small nodules;②Progressive massive fibrosis were found in 16 in stageⅡ(16/18) and in 22 in stageⅢ(22/22);③All cases showed fibrous strips and reticular opacities.Central fibers and vascular bundles thickening were found in 2 in stageⅡ(2/18) and in 5 in stageⅢ(5/22);8 in stageⅢ(8/22) cases showed obvious interlobular septal thickening and subpleural lines;④ 3 in stageⅡ(3/18) and 8 in stageⅢ(8/22)patients showed emphysema.(2)All cases except 4 in stageⅠshowed enlarged hilar and mediastinal lymph nodes,which contained silicon powder;2 in stageⅡ(2/18)and 9 in stageⅢ(9/22) patients showed fleck,mulberry,eggshell calcifications in the hilar lymph nodes.(3)Pleural thickening was showed in 6 in stageⅡ(6/18) and 13 in stageⅢ(13/22)cases,some of which showed typical bandlike pleural thickening.Conclusion CT features of silicosis in combination with clinical history will lead to accurate diagnosis.

Objective To study the dosimetry of mono isocenter irradiation technique in radiotherapy for nasopharyngeal carcinoma. Methods Conventional and mono isocenter irradiation techniques were used to simulate irradiation of the phantom and nasopharyngeal carcinoma patients with Siemens primus linear accelerator equipped with asymmetric collimators. Dose uniformity?isodose distribution and field overlap were measured at the plane of junction with TLD and film dosimeter on the phantom. The reproducibility of the set up was tested on patients with verification films. Results For an applied dose of 1?Gy, TLD dosimetry showed that the mean dose at the junction of fields were 1.01 ?Gy for the mono isocenter technique as compared to 1.09 1.13?Gy for the conventional technique. An ideal isodose distribution at the junction with mono isocenter technique was found by film dosimetry. The reproducibility of the set up was obviously better for the mono isocenter technique, with less field overlap (1mm) and set up deviation (0.5?mm) than those (6 14?mm and 3?mm) of the conventional technique. Conclusions This investigation of junction dosimetry confirms the potential advantage of the mono isocentric technique. Not only does it lead to superior dosimetry in the plane of junction but also associates with a better reproducibility of the set up.

Objective To evaluate the stability and compressive mechanical functions of the lumbar spine following insertion of a new self-made allograft interbody fusion cage.Methods Anti-bending intensity with three points test,anti-rotation intensity and compressive stiffness were measured at L4-L5 lumbar spine on five adult human fresh cadaveric specimens following insertion of a new self-made allograft interbody fusion cage and compared with that of before and after nucleus pulposus removal.Results The anti-bending intensity of flexion and extension of lumbar spine after inserting a new allograft interbody fusion cage was increased significantly(P

Objective To evaluate the reliability of ultrasonography used to guide the selection of uncuffed endotracheal tube (ETT) size for pediatric patients.Methods Eighty pediatric patients requiring endotracheal intubation for elective surgery under general anesthesia,aged 2-6 yr,of American Society of Anesthesiologists physical status Ⅰ or Ⅱ,were randomized into 2 groups (n=40 each) using a random number table:control group and ultrasonography group.In control group,the internal diameter of an uncuffed ETT was determined according to age-based formulas.In ultrasonography group,the outer diameter of an uncuffed ETT was determined according to the transverse diameter of the subglottic airway at the level of the cricoids cartilage measured by ultrasonography.The air leak test was performed after intubation,and either a larger or a smaller size of ETT selected was considered as a failure of intubation.The failure of intubation and postoperative complications related to intubation were recorded.Results Compared with control group,the total failure rate of intubation and failure rate due to the smaller size of ETT selected were significantly decreased in ultrasonography group (P＜0.01).There was no significant difference in the incidence of intubation-related complications between the two groups (P＞0.05).Conclusion Ultrasonogra-phy can be used to guide the selection of ETT size for pediatric patients.