Objective:To observe the impact of the Jiawei Xiaochaihu particle and it's prescription on immune organ and immune function of ITP mice.Methods:The ITP mice were divided into five groups at random:(1)Nomal group;(2)ITP model group;(3)Jiawei Xiaochaihu particle group;(4)Xiaochaihu particle group;(5)Danshen and Sanqi particle group.All ITP mice were administrated 7days later by 30ml per kilogram the rat's weight once a day for 14 days.21 days after the model mice were made,peripheral blood T lymphocyte subpopulation was detected with flow cytometry.Antibody against platelets was measured by ELISA.Peripheral blood IL-2 and IL-10 were measured by radio immuno assay(RIA).Thoracic gland and spleen were weighted to compute the immune organ index.Results:Spleens of ITP mice had intumesce of spleens and a rise of the index;Peripheral blood platelet antibody was also increased;A disproportion was found in T lymphocyte subpopulation,CD8 upgrade,CD3 and CD4 descent,CD4/CD8 also descent.As one of the Th1 cytokine,IL-2 expression accentuated while IL-10,the kind of Th2 cytokine,was inhibited.There were significances when compared with the nomal group(P0.05).Conclusion:There are indeed dysfunction in immune organ and its function of ITP mice.Jiawei Xiaochaihu particle and Xiaochaihu particle can exert a regulation,but the Danshen and Sanqi particle not.

Objective To observe the effect of Tongbi Prescription on pathomorphological changes of ankles in adjuvant arthritis rats. Methods Rats were randomly divided into six groups: normal control group,model control group,three Tongbi Prescription treated group (respectively with high-,mid-and low-dosages),Tripterygium glycosides treated group (each n=10). Except for the rats of normal control group,the others were injected with Freund’s complete adjuvant. The pathomorphological changes of ankles of each group were measured. Results The pathomorphological changes of high-and mid-dosages Tongbi Prescription treated group and Tripterygium glycosides treated group were lightened than those of model control group obviously (P

Objective To study the effect of Radix Salviae Miltiorrhizae (RSM), a kind of Chinese Traditional Medicine which can dissolve stasis by activating blood circulation, on proliferation, invasion, adhesion, migration and metastasis of B16-BL6 metastatic mouse melanoma cells and discuss its functional mechanism. Methods The proliferation, adhesion, invasion and migration capacity of B16-BL6 metastatic cells was evaluated by MTT assay, adhesion assay and reconstituted basement membrane invasion and migration assay in vitro respectively. Mouse spontaneous melanoma model was used to study the effect of RSM on metastasis in vivo. Results The extract of RSM bidirectionally adjusted the multiplication of B16-BL6 cells, promoted prominently the adhesion of B16-BL6 to Laminin, inhibited significantly B16-BL6 invading reconstituted basement membrane and the migration of B16-BL6. In the mouse spontaneous melanoma model, it suppressed significantly the volume of lung metastatic nodes but had little effect on the number of lung metastatic nodes. Conclusion The extract of RSM can alleviate the degree of the metastasis of B16-BL6 metastatic mouse melanoma cells, which may be related with inhibiting the B16-BL6 cells invading the extracellular matrix and reducing the migration of B16-BL6 cells.

Objective To observe the effect of Kouchuangping oral liquid (KCP), which can clear away stomach-heat, purge fire, nourish yin, on golden yellow hamster aphtha that induced by PQ. Methods Inject PQ which can cause membrana mucosa ulceration in golden yellow hamster’s cheek pouch, observe the pathomorphological process of ulcerative lesion and antioxidation of three different KCP dosage teams. Result The KCP has the function of anti-inflammation and anti-exudation on ulcerative lesion, which can relieve epithelium necrosis and promote the recovery of ulceration. It also can increase the vigor of SOD in serum and degrade the content of MDA. Conclusion KCP has the functions of antioxidation and eliminate oxydic and metabolic products.

Purpose To observe the effect of Xiange Granule on osteoporosis in ovariectomized rat model and explore the mechanism of prevention and treatment of osteoporosis.Methods 60 female rats were randomly divided into blank control group, model group, Xiange Granule low, middle and high-dose group, CEE3 group of 6 groups. The method used ovariectomy made animal model of postmenopausal osteoporosis. Gavage after 3 months measured femur bone mineral density, calcitonin, bone gla protein and estradiol concentration, observed cancellous bone of femur with electron microscope.Results Xiange Granule could significantly increase the bone mineral density, calcitonin, bone gla protein and estradiol levels of the ovariectomized rat model and improve the microstructure of cancellous bone.Conclusion Xiange Granule is effective for prevention and treatment on osteoporosis.

Objective:To compare and analyze the ultrasonic images between intestinal involvement in Kawasaki disease (IIKD) and abdominal type allergic purpura (ATAP), so as to improve the understanding of IIKD.Methods:From July 2014 to January 2020, the ultrasonographic images of 21 children with IIKD and 24 children with ATAP in Tianjin Children′s Hospital were collected and analyzed.Results:Univariate analysis showed that there were significant differences between IIKD group and ATAP group in non stratified thickening of intestinal wall, decreased echo of serosa and adjacent mesentery (irregular shape), thickening of peripheral fat tissue and abnormal shape of adjacent lymph nodes (all P<0.05); Multivariate analysis showed that decreased echo of serosa and adjacent mesentery (irregular shape) were independent influencing factors of IIKD. The area under ROC curve was 0.914(95% CI=0.819-1.000, P<0.001). The sensitivity and specificity of IIKD were 95.2% and 87.5%, respectively. Conclusions:The ultrasonographic images of intestinal involvement in IIKD and ATAP have certain specificity. Decreased echo of serosa and adjacent mesentery (irregular shape) can effectively distinguish IIKD and ATAP.

On the teaching of chemistry for medical majors on fewer lesson periods,the contents for teaching were reorganized and optimized.The students interests in learning basic chemistry were stimulated and their study on their own was guided by the interesting and heuristic methods.Thus,the comprehensive ability of medical students was cultivated and improved.

Objective To analyzed the CT features of struma ovarii(SO).Methods The clinical data and CT features of eleven patients with pathologically proved SO were retrospectively analyzed.CT features were compared with pathological results.Results(1)All tumors were unilateral.On non-enhanced CT,the lesions presented as well-defined irregular masses,which were cystic-solid(n=6,54.5%)or cystic(n=5,45,5%).(2)The cystic portions presented as well-defined,multiple,various size,and there were entire cystic walls with smooth inner wall.The tumors(63.6%)showed a high at attenuation lesions in the cyst portion of the mass on precontrast scans and the attenuation ranged from 72.3 to 113.5 Hounsfield units(HU)in 7 cases.The solid portions showed irregular tissue density,and were often distributed in the cysts.After contrast administration,the cystic portions showed no enhancement,the solid portions marked enhancement,and the cystic walls demonstrated no moderate,or marked enhancement.(3)The tumors(72.7%)showed stippled calcification in solid portions and(or)cystic wall in 8 cases.(4)The tumors(27.3%)accompanied with mature cystic teratomas,and showed fat density in 3 cases.(5)The tumors(27.3%)accompanied a great of ascites and pleural liquid.Conclusion CT characteristics of SO might be of great value for the diagnosis.

Objective To observe the anatomical and functional changes in patients with different degrees of myopic traction maculopathy (MTM) after vitrectomy.Methods It was a retrospective case series study.Forty-seven consecutive patients (47 eyes) diagnosed with MTM were studied between January 2010 and May 2014.There were 38 females (38 eyes) and 9 male (9 eyes),mean age was (65.13±6.98) years,mean axial length was (29.23±1.77) mm.The eyes was divided into 3 groups according to the macular morphologies on optical coherence tomography (OCT),including macular retinoschisis only group (MRS group,18 eyes),foveal retinal detachment group (FD group,13 eyes) and full-thickness macular hole group (MH group,16 eyes).All the eyes underwent minimum resolution angle in logarithmic (logMAR) best corrected visual acuity (BCVA),intraocular pressure,axial length,A or B-ultrasonography,fundus photography,OCT and microperimetry examinations.The average logMAR BCVA of 47 eyes was 1.43±0.52.The center retinal thickness (CRT) of eyes in MRS and FD group was (528.45± 167.61) μm.All the patients underwent pars plana vitrectomy combined with internal limiting membrane peeling.The mean follow-up period was 23.4 months.The changes of logMAR BCVA,microperimetry and macular microstructural were observed.Results At the final follow-up,the logMAR BCVA of 47 eyes was 0.86 ± 0.42,which improved compared with the preoperative vision (t=7.36,P＜ 0.001).The mean CRT of eyes in MRS and FD group was (250.90 ± 91.81) μm,which improved compared with the preoperative CRT (t=8.17,P＜0.001).In MRS group,the retina was attached in 18 eyes.In FD group,the retina was attached in 11 eyes,MH was observed in 2 eyes.In MH group,recurrent retinal detachment was observed in 1 eye.The differences of logMAR BCVA and retinal sensitivity among MRS,FD and MH groups were significant (x2 =6.38,11.08;P=0.030,0.004).Conclusion The macular structural and visual function in MTM eyes with macular retinoschisis only after vitrectomy are better than those in MTM eyes with MH and foveal retinal detachment.

LTB gene fragment was amplified by PCR from plasmid pMDTLT, and a recombinant plasmid pETLTBVP1 was constructed by inserting LTB gene fragment into VP1 gene expression plasmid pETVP1 constructed previously. The recombinant plasmids were transformed into E. coli BL21(DE3) and induced to express by IPTG. The recombinant protein existed in the inclusion body and its molecular weight was about 39 kD proved by SDS-PAGE analysis. Western blotting showed that the fusion protein could be reacted with both anti-FMDV and anti-cholera toxin serum demonstrating the immunoactivity of the fusion protein. Strong immune responses can be induced in mice inoculated with the fusion protein intraperitoneally, and the serum antibody level is higher than that of commercial foot-and-mouth disease vaccines.
Animals ; Antibodies, Viral ; blood ; Bacterial Toxins ; genetics ; immunology ; metabolism ; Capsid Proteins ; genetics ; immunology ; metabolism ; Enterotoxins ; genetics ; immunology ; metabolism ; Escherichia coli ; genetics ; metabolism ; Escherichia coli Proteins ; Female ; Gene Fusion ; genetics ; Mice ; Plasmids ; genetics ; Recombinant Fusion Proteins ; genetics ; immunology ; metabolism