Objective:To investigate the promoting effects of interleukin-13(IL-13)on the proliferation of airway smooth muscle cells(ASMC)in mice and its mechanism.Methods:Murine ASMC were isolated and subcultured.IL-13,IL-13plus AG1478,IL-13plus neutralizing antibody to TGF-?,IL-13plus neutralizing antibody to EGF,IL-13plus neutralizing antibody to HB-EGF,IL-13plus dexamethasone were added to the media,respectively.MTT and 3 H-TdR incorporation assays were used.Apical media were analyzed for the presence of soluble TGF-?using ELISA.Results:The D 595 value of MTT assay and the cpm value of 3 H-TdR incorporation assay of IL-13group were significantly higher than those of control group(P

Objective To investigate the value of CT in the diagnosis of dedifferentiated liposarcoma.Methods Fifteen surgical operations of dedifferentiated liposarcoma in 11 patients, confirmed by pathology, underwent plain (n=15) and contrast enhanced (n=12) CT scans. Two of the 12 lesions underwent dynamic multi-phase contrast scans. The CT features and the corresponding pathological basis were analyzed.Results The lesions were divided into 3 groups according to the fatty component and its distribution. The CT appearances were as follows: (1)a well delineated fatty component in the lesion and a closely apposed non-fatty region in 10 cases including 5 initial (5/5, 100%) and 5 recurrent (5/10, 50%), which depicted an abrupt transition from the well-differentiated area to the high-grade sarcoma component under microscope; (2)fatty component within the lesion was less than 10% in 3 recurrent (3/10, 30%); (3)fatty masses and non-fatty masses separately distributed in 2 recurrent cases (2/10, 20%). Contrast enhanced CT scan showed moderate to marked inhomogeneous enhancement in the early phase in all 12 lesions, and delay enhancement was also observed.Conclusion Dedifferentiated liposarcoma exhibited specific appearances as well delineated fatty component and closely apposed non-fatty region with apparent enhancement. Dynamic contrast CT scans play important roles in the diagnosis and differential diagnosis of dedifferentiated liposarcoma, and help pre-surgical grading and pathological sampling.

Objective To explore the correlation between cardiovascular risk factors and characteristics of coronary artery le‐sions ,the degree of stenosis in patients with coronary artery disease(CAD) .Methods Totally 156 patients who under went coro‐nary angiography(CAG) in our hospital were divided into CAD group(n=98) and non‐CAD group(n=58) which the patients with coronary stenosis <50% according to the result of CAG .Their clinical data were retrospectively analyzed ,including gender ,age , body mass index(BMI) ,family history ,smoking status ,left ventricular ejection fraction(LVEF) ,blood lipid ,blood pressure and blood sugar levels ,the correlation between cardiovascular risk factors and coronary artery lesions was analyzed .Results There was significant difference of gender ,systolic blood pressure (SBP) ,total cholesterol (TCH) ,three acyl glycerin (TG) ,low density lipo‐protein cholesterol (LDL‐C) ,fasting blood glucose (FBG) ,smoking between the two groups(P<0 .05) ,but no significant differ‐ence was found at the level of ages ,BMI ,high‐density lipoprotein cholesterol (HDL‐C) ,diastolic blood pressure (DBP) ,family his‐tory ,serum creatinine (Cr) ,LVEF between the two groups(P>0 .05) .The number of coronary artery lesion branch were increased with these risk factors incresed ,such as smoking ,SBP ,LDL‐C ,FBG ,no significant difference with age ,male ,TCH ,TG .The degree of stenosis were increased with SBP ,FBG ,no significant difference with age ,male ,smoking ,TCH ,TG and LDL‐C .Conclusion The smoking ,males ,FBP and SBP are the prominent risk factors of CAD in Qinghai area ,prosmote the occurrence and development of CAD together .

Objective To study the changes in cortical activation of frontal lobes and temporal lobes between schizophrenic patients and comparison subjects during a verbal fluency task by using functional magnetic resonance imaging (fMRI). Methods Thirteen schizophrenic patients and 8 volunteers were studied during a verbal fluency task performance. fMRI of frontal lobes and temporal lobes was conducted at a GE 1.5 T Signa Horizon LX scanner while the subjects performed the task. Stimulus sequences were divided into six 30-second segments by using a task-activation paradigm that alternated between resting and stimulated states. Results Schizophrenic subjects demonstrated significantly less left frontal activation than comparison subjects during a word fluency task, and comparison subjects showed evidently more activation in left frontal lobes than in the right (P0.05). Conclusion These preliminary data suggest that fMRI has the sensitivity to detect the differences in activation between comparison subjects and schizophrenic patients during higher cortical functions.

Objective To investigate the effectiveness and safety of minimal invasive surgery combined with ventriculoscope for the treatment of basal ganglia hemorrhage.Methods From June 2014 to June 2015 there were 57 patients with basal ganglia hemorrhage were selected into this study.These patients were signed into the minimal invasive surgery combined with ventriculoscope group and the craniotomy group according to the methods of surgery they received.And the clinical outcomes of the two groups were compared.Results Compared with the craniotomy group,patients in the minimal invasive surgery combined with ventriculoscope group got a significantly lower Glasgow coma scale at 7 days,14 days and 28 days after the operation (P =0.02,0.04,0.04);the hospital stays were significantly reduced in the minimal invasive surgery combined with ventriculoscope group [(21.45 ±5.67)d vs.(25.67 ±7.45)d,P =0.02];and the operation time were significantly reduced as well [(134.45 ±21.11)min vs.(178.65 ±45.32)min,P =0.000)].There was no significant difference in intra-cranial pressure,rate of hematoma clearance,rate of organ functional failure,rate of re-bleeding and mortality 28 days after operation (P >0.05).Conclusion The minimal invasive surgery combined with ventriculoscope is effective and safe for the treatment of basal ganglia hemorrhage,which is worthy of popularization.

Objective:To investigate gypsum fibrosum from different habitats by scanning electron microscopy ( SEM) . Methods:Gypsum fibrosum samples from different habitats were scanned by an electronic microscope. Results: The results of SEM showed that gypsum fibrosum (CaSO4·2H2O) from different habitats was with the crystal structure of fibrous,tabular or flaky aggregation. The sur-face amplification of gypsum fibrosum from Hubei showed obvious black spots,while that from the other habitats was without the feature. Conclusion:The method is simple and accurate, which can be used to investigate gypsum fibrosum from different habitats.

Objective To investigate the changes of nuclear factor-κB (NF-κB),tumor necrosis factor-α(TNF-α),and cell apoptosis of cerebral ischemia-reperfusion injury and the influence of brain-derived neutrophic factor(BDNF) on these parameters in rats.Methods Eighty-four male Sprague-Dawley (SD) rats were randomly divided into two groups:BDNF (n =42) and control (n =42) groups.The BDNF group was induced using the improved Zea-longa method and were received abdominal injections of BDNF (0.5 μg/μl) immediately after injury.The control group was received abdominal injections with the same dose sodium chloride injection immediately after injury and repeated one time everyday until the rats was killed.Each group was divided into seven subgroups by sacrificed time after injury,including subgroups 1 h,3 h,6 h,12 h,24 h,3 d,and 7 d; each subgroup got 6 rats.Each subgroup were randomly selected three rats after being killed.The expressions of NF-κB and TNF-α of rats contusion peri tissues brain tissue were detected by immunohistochemical methods.Terminal deoxynucleotidyl transferase (TUNEL) method was used to observe the peri cell apoptosis after brain contusion.Results The expressions of NF-κB and TNF-α in BDNF group was significantly decreased relative to the control group (P ＜ 0.05),with a significant positive correlation between two parameters in two groups (P ＜ 0.001).The number of apoptotic cells was significantly decreased in the BDNF group relative to control group (P ＜ 0.05).Conclusions Brain-derived neutrophic factor probably relieves inflammation response,reduces the change of secondary brain injury after traumatic brain injury,and decreases neural cell apoptosis,and finally provides protection of neurocytes.

Background and purpose:Histological grade of DCIS is an important factor in the prognosis, high nuclear grade have higher inifltration trend and recurrence rate. This study aimed to evaluate the dynamic and morphological MRI charactristics of ductal carcinoma in situ (DCIS) of the breast, then analyze its relations with nuclear grade. Methods:Of the 94 patients, 97 lesions were proved DCIS by pathology. The morphology, maximum size, time-intensity curve of lesion were recorded or measured. Statistic was performed to identify MR imaging features that optimally discriminated HNG from non-HNG DCIS. Results:There were 49 non mass-like enhancement lesions, and 48 masses enhancement lesions. Among 49 non mass-like lesions, 29 were segmental enhancement (59.18%). Dynamic enhanced performance:50 exhibited plateau curves and 27 were washout curves. BI-RADS categories:22 BI-RADS 4A, 19 BI-RADS 4B, 29 BI-RADS 4C, and 27 BI-RADS 5. HNG lesions exhibited larger mean maximum lesion size (non-mass-like enhancement: P=0.01; mass: P=0.03), time- intensity curve was variable to approach signiifcance (P=0.01), and BI-RADS can help to discriminate the nuclear grade (P=0.02). There were no differences in lesion morphology (P>0.05). Conclusion:The preliminary ifndings suggest that DCE MR imaging features may aid in identifying patients with high risk DCIS.

Objective To investigate the long-term results of gastrocnemius fascial turn-down flaps and suture anchors in reconstruction of chronic Achilles tendon rupture.Methods From September 2007 to February 2010,20 patients with chronic Achilles tendon rupture were reconstructed with gastrocnemius fascial turn-down flaps plus suture anchors.There were 13 males and 7 females at mean age of 36 years (range,23-50 years).Unilateral tendon rupture was observed in 19 patients and bilateral in 1 patient.Tendon defect gap was 5-9 cm (mean 6.7 cm).Braces were used to immobilize the lower limber,and early exercise was encouraged.The outcomes were assessed using Arner-Lindhohm scoring system and Leppilahti scoring system.Results Postoperative recovery was uneventful.No patients experienced cut infection or other complications.After a mean 38-month follow-up (range,30-42 months),all patients could stand on the injured toes.Muscular strength of the affected lower limber was restored to 89％ of the unaffected side.According to the Arner-Lindhohm score,10 patients were rated as excellent and ten good.Mean Leppilahti score was 85 points (range,60-100 points),with excellent results in nine patients,good results in 10 and fair result in one.Conclusion For patients with chronic Achilles tendon rupture,the reconstructing using gastrocnemius fascial turn-down flaps combined with suture anchors can attain rigid fixation,early functional exercise,effective prevention of tendon adhesion,and good function recovery.

Clinical characteristics were retrospectively analyzed in a patient with parathyroid crisis as the main symptoms of parathyroid adenoma and asymptomatic pheochromocytoma.This analysis was aimed to implement specific diagnosis and treatment and to accumulate experience in managing these diseases.