Objective To explore the effect of Jin's three-needle therapy, as an assistant treatment, in improving the intelligence of children with mental retardation (MR).Method Seventy-six MR patients were divided into a control group and an observationgroup, 38 cases in each group.The control group was treated with rehabilitation, while the observation group with JIN's three needle therapy plus the rehabilitation training.Prof. JIN Rui's 10-item observation index was adopted to evaluate the memory ofthe patients before and after the treatment; Gesell Developmental Schedule (GDS) was used to measure the intelligence before and after the treatment. The clinical efficacies in the two groups were statistically analyzed.Result After the treatment, Prof.JIN Rui's 10-item index scores dropped obviously and the GDS scores increased significantly in both groups, while the decrease and increase of scores in the observation group were more significant than those in the control group (P<0.05). The total effective rate was 94.7% in the observation group, significantly higher than 71.1% in the control group (P<0.05).Conclusion As an assistant treatment, JIN's three-needle therapy can help rehabilitation training to improve the intelligence of MR patients.

Objective To observe the effect of Tongbi Prescription on pathomorphological changes of ankles in adjuvant arthritis rats. Methods Rats were randomly divided into six groups: normal control group,model control group,three Tongbi Prescription treated group (respectively with high-,mid-and low-dosages),Tripterygium glycosides treated group (each n=10). Except for the rats of normal control group,the others were injected with Freund’s complete adjuvant. The pathomorphological changes of ankles of each group were measured. Results The pathomorphological changes of high-and mid-dosages Tongbi Prescription treated group and Tripterygium glycosides treated group were lightened than those of model control group obviously (P

Objective To study the effect of Brucea javanica oil emulsion on apoptosis of bladder cancer J82 cells and the possible mechanism.Methods Brucea javanica oil emulsion of different concentrations (0.5～4.0 ml/L) was added to the bladder cancer J82 cell cultured in vitro.After 24 h, the proliferation of J82 cells was measured by methyl thiazole tetrazolium (MTT) assay, cell apoptosis was detected by morphological observation and flow cytometer analysis, and caspase-3 activity was assessed using a caspase-3 kit. Results 0.5～4.0 ml/L Brucea javanica oil emulsion inhibited J82 cell proliferation in a dose-dependant manner.2.0 ml/L Brucea javanica oil emulsion induced typical apoptosis 24 h after incubation. The caspase-3 activity of 2.0 ml/L Brucea javanica oil emulsion group was higher than those of control group or Brucea javanica oil emulsion plus Ac-DEVD-CHO group. Conclusion Brucea javanica oil emulsion induces J82 cell apoptosis, and this effect includes activation of caspase-3.

Purpose To observe the effect of Xiange Granule on osteoporosis in ovariectomized rat model and explore the mechanism of prevention and treatment of osteoporosis.Methods 60 female rats were randomly divided into blank control group, model group, Xiange Granule low, middle and high-dose group, CEE3 group of 6 groups. The method used ovariectomy made animal model of postmenopausal osteoporosis. Gavage after 3 months measured femur bone mineral density, calcitonin, bone gla protein and estradiol concentration, observed cancellous bone of femur with electron microscope.Results Xiange Granule could significantly increase the bone mineral density, calcitonin, bone gla protein and estradiol levels of the ovariectomized rat model and improve the microstructure of cancellous bone.Conclusion Xiange Granule is effective for prevention and treatment on osteoporosis.

Objective To investigate the significance of digital tomosynthesis (DTS) for suspicions lesions in the physical examination of the chest.Methods Totally 1 000 physical examinees were divided into two groups,with 500 younger ones in one group and the remained 500 ones in the other.The examinees underwent examination with digital X-ray radiography,and then the suspicious cases went through DTS examination to analyze the detection rate of pulmonary positive results.Results There were 110 suspected cases found by digital X-ray radiography,including 8 young ones and 102 old ones.The suspected cases went through DTS examination,and totally 92 nodules were found including 4 ones in the young persons and 88 ones in old persons.CT examination found 89 carcinomatous nodules in the 92 ones.Conclusion DTS examination can detect the lesion of the physical examinee,and lays a foundation for early diagnosis and treatment.

Objective To explore the improvement effect of SLOT Scan technology (narrow seam exposure capture technology) and the radiography techniques on the quality of the scoliosis X-ray films in teenagers. Methods The Sonialvision Satire Ⅱ equipment of Shimadzu corporation and SLOT Scan were applied to take the radiography for 60 patients taller than 1.50 metres. All the data were collected through a continual exposure, and the images were sewn up through a seamless connec-tion software. Results Cervical, thoracic, and lumbar and sacral segments could clearly present at the same time on one X-ray film by seamless splicing, and the quality of one time radiograph was as good as one film. Conclusion As a new radiology technique, SLOT Scan can wipe off splitting arti-fact effectively and make the whole spine seamless present on one X-ray film. It helps the spinal sur-geons to observe, calculate and measure accurately. It is useful to choose the operation mode and judge the curative effect.

OBJECTIVE:To establish a rapid,accurate and standardized DNA barcode identification method for the ferulic me-dicinal plants. METHODS:Genomic DNA was extracted from Ferula sinkiangensis K. M. Shen and Ferula fukanensis K. M. Shen,ITS2 sequences were amplified and sequenced,analytical similarity search method was conducted,13 species 26 samples gene ITS2 sequences of ferulic medicinal plants in GenBank database were downloaded and comparatively analyzed;interspecies and intraspecies genetic distances were calculated,and phylogenetic tree was developed for cluster analysis. RESULTS:According to calculation,species genetic distances of the 15 species ranged 0.009-0.230,average genetic distance was 0.018;results of clus-ter analysis showed DNA barcode ITS2 sequences can cluster the 32 kinds of ferulic medicinal plants into different classes. CON-CLUSIONS:The established DNA barcode ITS2 sequences identification method can accurately and rapidly identify the genuine and false samples of ferulic species.

Objective To investigate the effect and the mechanism of Sorcin (soluble resistancerelated calcium-binding protein )on development of drug-resistance in human gastric cancer cell line.Methods The full length Sorein cDNA was isolated by reverse transcription-polymerase chain reaction (RT-PCR).The FLAG-sorein-peDNA 3.1 plasmid was constructed by directed cloning of Sorein gene into the eukaryotie expression vector FLAG-peDNA 3.1,and was transfeeted into SGC7901 cells using liposome-mediated method.The expressions of Sorcin mRNA and protein in stable clone were detected by RT-PCR and Western blot. The intracellular concentration of Vineristine (VCR) in Sorein-transfected SGC7901 eells(SGC7901-F-Sor) and SGC7901 cells with or without Verapamil(VRP) was determined by high performance liquid chromatography(HPLC).Results The FLAG-Sorcin-peDNA3.1 plasmid vector was constructed successfully by DNA recombination and was transfected into SGC7901 cells.RT-PCR and Western blot analysis revealed that the expressions of Sorcin mRNA and the protein were up-regulated in SGC7901 F-Sor cells.Compared to the parent SGC7901 cells,the concentration of VCR in SGC7901-FSor cells was decreased by 76.89%,but it was increased by 2.41 times when treated with VRP.Conclusions Overexpression of Sorcin in SGC7901 cells results in decreasing concentration of VCR,which indicate that Sorcin may play a role in drug-resistance of SGC7901 cells by regulating the transference of chemotherapy drugs.The effect of Sorcin can be reversed by VRP.

Objective:To explore the relationship between the accelerated repopulation of tumor cells in the afterloading fractionated radiation therapy and the radiation dose,Ki67 antigen,PcNA & mutation p53 antigen expression were analyzed by continual biopsy on the same patients of cervical carcinoma two weeks (10 Gy)and four weeks(20 Gy).Methods:Ki67 antigen expression and cell cycle analysis (SPF,PI,DEN) were checked by flow cytometry,PCEA & mutation p53 antigen expression were checked by means of immunohistochemical technique and computerized image cytometry.All these indexes were viewed on their relationship with the irradiation dose.Rusults:Ki67 antigen expression and DEN were increased as radiation dose increased.The difference of Ki67,PCNA and mtP53 antigen expression at the dose of 10 Gy had statistical significance compared with before brachytherapy,but it had no statistical significance compared with at the dose of 20 Gy.Conclusion:It may be concluded that the tumor cell repopulation had occurred at the dose of 10 Gy (two weeks after brachytherapy).But these markers were not obviously changed compared with at the dose of 20 Gy,so it leads to a conclusion that tumor cell repopulation was stable.It also offers the theory basis to select the opportunity of accelerated brachytherapy of cervical carcinoma.

OBJECTIVE:To establish a method for the content determination of eudesmin in Zanthoxylum simulans. METH-ODS:HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-water(56:44,V/V)at flow rate of 1.0 ml/L,detection wavelength was 221 nm,column temperature was 25 ℃. RESULTS:The linear of eudesmin was 0.208-2.08 μg (r=0.999 8);RSDs of precision,reproducibility and stability tests were lower than 2.0%;recovery was 96.18%-103.04%(RSD=2.58,n=6). CONCLUSIONS:The method is simple and good reproducibility,and can be used for the content determination of eu-desmin in Z. simulans.