Objective To compare the detection results and estimate the bias of two hematocyte analyzers of different brands . Methods Sysmex XE‐2100 hematocyte analyzer and Abbott CD‐1700 hematocyte analyzer were chosen to be the reference instru‐ment and comparison instrument ,respectively .40 cases of fresh whole blood samples were collected for the detection of WBC ,RBC , HGB ,HCT ,MCV ,MCH ,MCHC ,and PLT respectively by the two instruments .The correlation coefficient (r) ,regression equa‐tions and bias were calculated and compared to determine the comparability of the two instruments .Results The precisions of two hematocyte analyzers were satisfactory .When fresh whole blood was used as calibrators ,the results of all eight items of the two in‐struments had good correlations (r>0 .975) ,and the relative bias was acceptable .Conclusion The results of two hematocyte ana‐lyzers are comparable .With fresh whole blood using in the comparison test between different hematocyte analyzers ,systematic er‐rors can be discovered in time .

OBJECTIVE: To optimize the prescription of traditional Chinese medicine for motion sickness (MS).METHODS: Zingiber officinale,Herba pogostemonis,Aucklandia lappa were extracted respectively,and rotating-inducing MS mice were enrolled in uniform design.The prescription was optimized with MS index as the parameters.RESULTS: The optimal prescription was as follows:60 g Z.officinale,45 g Herba pogostemonis,5 g A.lappa.The extractive of prescription was significantly better than dimenhydrinate in the treatment of MS.CONCLUSION: MS index is a stable and sensitive parameters and it is suitable for screening and evaluation of anti-MS drugs.R.zingiberis,H.pogostemonis,A.lappq are potential drug for MS.

Objective To analyze the GDM of 336 cases with chronic HBV in pregnancy. Methods According to HBV DNA≥1.0 × 103 IU/mL, participants were divided into HBV DNA (+) or (-) group. 409 cases without HBV were selected as control group. Differences on GMD incidence between groups and virus load and OGTT blood sugar correlation were compared. Results The incidence of GDM of HBV DNA (+) or (-) group was 16.77% and 17.71%, which is higher than that in HBV group (10.27%). The difference is significant (P < 0.05). The correlation index between HBV DNA and fasting blood-glucose is r = 0.005, P = 0.610, the result of which is not statistically significant. But correlation index between HBV DNA and blood sugar at 1 h , 2 h are r = 0.082, 0.086; P = 0.000, 0.000, the result of which is statistically significant. Conclusion The oc-currence of GDM were higher in HBV DNA (+) or (-) group. The viral load is positively related with blood sugar of glucose tolerance at 1 h or 2 h.

OBJECTIVETo study the predictive factors that are associated with intraoperative identification of the sentinel lymph node (SLN).

METHODSLymphatic mapping using blue dye was performed in 108 patients with stage I and II operable primary breast cancer. Subsequently the patients received operations of breast cancer including axillary dissection. Clinical and histological factors were assessed to determine those that were associated with intraoperative identification of the SLN.

RESULTSThe sentinel node was identified at the time of surgery in 84 patients (77.78%). Of the clinical factors assessed, age(y) < 50 (chi(2) = 7.447, P < 0.01), tumour in the upper quadrant (chi(2) = 6.330, P < 0.05), diagnosis by preoperative biopsy (chi(2) = 5.509, P < 0.05), successful mapping of the lymphatic duct (chi(2) = 13.125, P < 0.01) were significant in identifying the sentinel node at operation. No histological factor was associated with intraoperative identification of the sentinel node.

CONCLUSIONThere are the possibility of failure of SLN identification at sentinel lymph node biopsy. Our results suggest that the best predictor of intraoperative sentinel node identification is the visualization of the lymphatic duct on mapping by blue dye. Other factors such as age, tumour site as well as diagnostic method are also important in determining the success of the procedure.

Breast Neoplasms ; pathology ; Female ; Humans ; Intraoperative Care ; Lymph Nodes ; pathology ; Lymphatic Metastasis ; Middle Aged ; Neoplasm Staging ; Sentinel Lymph Node Biopsy

Objective To investigate whether long-chain non-coding ribonucleic acid HAGLR(LncRNA HAGLR)can affect lipopolysaccharide(LPS)-induced apoptosis and expression of inflammatory factors of retinal pigment epithelium(RPE)cells by targeted regulation of the expression of micro ribonucleic acid-625-5p(miR-625-5p),so as to lay an experi-mental foundation for revealing the mechanism of retinopathy.Methods LPS-induced human retinal pigment epithelial(ARPE-19)cells were set as the LPS group,and normally cultured ARPE-19 cells were assigned to the Con group.Quanti-tative real-time polymerase chain reaction was used to detect the expression levels of LncRNA HAGLR and miR-625-5p.Based on different transfection reagents,the cells were divided into the LPS+sh-NC group,LPS+sh-HAGLR group,LPS+miR-NC group,LPS+miR-625-5p group,LPS+sh-HAGLR+anti-miR-NC group,and LPS+sh-HAGLR+anti-miR-625-5p group.Flow cytometry was used to detect apoptosis rate;enzyme-linked immunosorbent assay was used to detect the lev-els of interleukin-6(IL-6)and interleukin-1β(IL-1β);the targeting relationship between LncRNA HAGLR and miR-625-5p was verified;Western blot was used to detect the protein levels of activated cysteinyl aspartate specific proteinase 3 and 9(cleaved-Caspase 3 and cleaved-Caspase 9).Results Compared with the Con group,the LPS group showed an increase in the expression level of LncRNA HAGLR and a decrease in the expression level of miR-625-5p(both P＜0.05),and there were increases in apoptosis rate,protein levels of cleaved-Caspase 3 and cleaved-Caspase 9,and levels of IL-6 and IL-1β(all P＜0.05).Compared with the LPS+sh-NC group,the LPS+sh-HAGLR group showed decreases in apoptosis rate,protein levels of cleaved-Caspase 3 and cleaved-Caspase 9,and levels of IL-6 and IL-1β(all P＜0.05);LncRNA HAGLR could negatively regulate the expression level of miR-625-5p(P＜0.05).Compared with the LPS+miR-NC group,the LPS+miR-625-5p group showed an increase in the expression level of miR-625-5p and decreases in apoptosis rate,protein levels of cleaved-Caspase 3 and cleaved-Caspase 9,and levels of IL-6 and IL-1β(all P＜0.05).Compared with the LPS+sh-HAGLR+anti-miR-NC group,the LPS+sh-HAGLR+anti-miR-625-5p group showed a decrease in the expression level of miR-625-5p and increases in apoptosis rate,protein levels of cleaved-Caspase 3 and cleaved-Caspase 9,and levels of IL-6 and IL-1β(all P＜0.05).Conclusion Interference on LncRNA HAGLR expression can realize the targeted regulation of miR-625-5p expression to inhibit the apoptosis and inflammatory factor expression,reducing LPS-induced injury of ARPE-19 cells.

Objective To investigate the anti-postmenopausal osteoporosis mechanism of Zhuanggu Zhi-tong Capsule ( Bone-strengthening Pain-relieving Capsule , ZGZTC) in regulating Treg/Th17 balance in bone tissue .Methods Seventy-two 8-month-old SD female rats were allocated randomly into sham oper-ation group, model group, estradiol group (0.21 mg/kg), ZGZTC high-dose group (1.28 g/kg), ZGZ-TC medium-dose group (0.64 g/kg) and ZGZTC low-dose group (0.32 g/kg).There were 12 rats in each group .Bilateral ovariectomy was performed on rats in all groups except for the sham operation group.After administration for 18 weeks, all rats were anaesthetized intraperitoneally with hydrazine hy-drate .The content of E 2 , IL-10 and IL-17 A was measured with ELISA in left femur .Gene expression of Foxp3, RORγt , IL-17A and IL-10 in right femur was detected by using RT-PCR, and the number of cell with RORγt or Foxp3 was counted with immunohistochemistry in left tibia .Results Zhuanggu Zhitong Capsule significantly increased E 2 and IL-10 levels in bone tissue (P<0.01), reduced the level of IL-17A in bone tissue (P<0.05 or P<0.01).It significantly increased the Foxp3 and IL-10 mRNA ex-pression and inhibited the RORγt mRNA expression in bone tissue (P<0.05).The percentage of cell with Foxp3 was significantly increased by ZGZTC in bone tissue ( P<0 .05 ) , and the percentage of cell with RORγt was significantly reduced (P<0.05 or P<0.01).The ratio of Foxp3 to RORγt presented a positive correlation with the E2 level (r=0.990 5,P<0.01).Conclusion Zhuanggu Zhitong Capsule could downregulate the number and function of Th 17, upregulate the number and function of Treg in bone tissue in ovariectomized rats partly via improving the E 2 level.

Objective To study the inhibitory effect of astragaloside IV (AST) on the apoptosis of PC12 cells induced by oxygen-glucose deprivation/oxygen-glucose rehabilitation (OGD/OGR) through activating autophagy.Methods PC12 cells in logarithmic phase were collected and randomly divided into normal group, OGD/OGR group (model group), AST solvent control group (DMSO group), AST group, AST+autophagy inhibitor group (AST+3-MA group), 3-MA group and autophagy activator group (RAPA group).Except of normal group, all other groups were given treating of OGD for 3 h and then OGR for 12 h and simultaneous medication treating.The morphological changes of autophagosomes were observed by using transmission electron microscope (TEM), expressions of LC3II, Beclin1 and Caspase-3 were detected by using Western blotting, and apoptosis of PC12 cells were detected by using flow cytometry after TUNEL staining.Results Compared with normal group, the typical autophagosomes were observed, expressions of LC3II, Beclin1 and Caspase3 increased, and apoptosis rate and apoptosis increased (P<0.05).Compared with model group, the quantity of autophagosomes and expressions of LC3II and Beclin1 increased (P<0.05), and expression of Caspase3 and apoptosis rate and apoptosis decreased in AST group and RAPA group (P<0.05).Compared with model group, the quantity of autophagosomes and expressions of LC3II and Beclin1 decreased (P<0.05), and expression of Caspase3 and apoptosis rate and apoptosis increased in 3-MA group (P<0.05), while DMSO group and model group had no significant difference (P>0.05).Compared with AST group, the quantity of autophagosomes and expressions of LC3II and Beclin1 decreased (P<0.05), and expression of Caspase3 and apoptosis rate and apoptosis increased in AST+3-MA group and 3-MA group (P<0.05).Conclusion AST play a neuroprotective role through activating autophagy and inhibiting apoptosis of PC12 cells induced by OGD/OGR.