Objective To explore the clinical significance of changes of serum IL-6,IL-8 and adiponectin in patients with acute cerebral infarction(ACI).Methods Serum IL-6,IL-8 and adiponectin levels were measured by ELISA in 98 patients with ACI and 80 healthy control.Results The ACI group serum IU6 and IL-8 levels were higher than the healthy control group(all P＜0.01),while the level of adiponectin was converse;The levels of serum IL13,IL-18 increased gradually with the increase of the severity and the volume of ACI,while the level of adiponectin was decreased;the serum level of adiponectin was negative correlated with the level of IL-6、IL-8 in patients with ACI (r=0.733,0.715,all P＜0.05),the serum level of IL-6 was positive correlated with the level of IL-8(r=0.830,P＜0.01).Conclusion The serum IL-6,IL-8 and adiponectin could reflected situation of patients and extent of neurologic impairment,which had close relationship with the clinical process of ACI and had significance value to evaluate the pathogenetic condition and prognosis.

Objective To investigate the quality of Pulsatilla chinensis from different sources on reference of pulchinenoside B4 as the index. Methods Five kinds of Pulsatilla chinensis herbs and cut crude drug were purchased from Chinese herbal medicine market in Bozhou,and Wild Pulsatilla chinensis were collected from Chuzhou. The content of pulchinenoside B4 were determined by HPLC. Results Linear relationship was good within 0.312 5～10 ?g,the regression equation was Y=276 350X -12 180,R2= 0.999 6. Conclusion The differences of pulchinenoside B4 from different sources of Pulsatilla chinensis varied greatly.

stir-baked Radix Paeoniae Alba with vinegar. Conclusion Different processing method has great influence on the paeoniflorin content of Radix Paeoniae Alba.

Objective To optimize the extracting conditions of polysaccharide from Imperata Cylindrica and establish a suitable method for its content determination. Methods Based on the qualitative yield of polysaccharide extraction, a orthogonal test was employed to evaluate the effect of four factors including extracting temperature, extracting time, the extraction times and the ratio of material to liquid. The phenol-dense sulphuric acid method was applied for the content determination of polysaccharide. Results The times of extraction and the extracting temperature influenced the content of polysaccharides mostly, while the duration of extraction and the amount of water showed little influence. Conclusions The optimum extraction condition was refluxing and extracting for 3 times at 85 ℃ for 3 hours, and with 15 folds of water for every time. The contents of polysaccharide in Imperata Cylindrica collected from two different places were above 0.15%.

Objective: To optimize the alcohol-extraction process and condition of Radix Pulsatillae.Methods: Alcohol concentration,the extraction time and number of extraction on effect of extraction rate of pulchinenoside B4 were evaluated by orthogonal design L9(34).Results: The optimum condition of the alcohol extraction was: 75% ethanol,3 times of extraction,3h for extraction process.Conclusion: The extraction method can be used for reference for the extraction process of Radix Pulsatillae.

Objective:To assess the nutritional status of obese patients prior to bariatric surgery, and to explore the related factors of nutrient deficiency.Methods:Clinical data of 43 patients with obesity who underwent bariatric surgery at Beijing Hospital from Jan. 2011 to Dec. 2017 were retrospectively analyzed. Gender, age, BMI, body composition analysis data, blood test data of nutrients were collected. The software of SPSS 20.0 was used to conduct data analysis.Results:Nutrients deficiencies were found for vitamin D (100.0%), iron (26.1%), prealbumin (15.4%) and hemoglobin (7.0%). Hemoglobin, prealbumin, and serum iron levels were significantly higher in male patients than in female patients ( P=0.001, 0.000 and 0.001, respectively). Body fat percentage was negatively correlated with 25 hydroxyvitamin D ( r=-0.983, P=0.017), and positively correlated with serum sodium ( r=0.568, P<0.001). Conclusions:Obesity patients were presented with a variety of nutritional deficiencies before bariatric surgery. A comprehensive nutrients test should be performed before bariatric surgery, to detect and correct nutrient deficiencies preoperatively.

Objective To investigate the changes of the neuron-specific enolase(NSE) and soluble intercellar adhesion molecule-1(CAM-1) in serum and cerebrospinal fluid(CSF) in the patients with viral encephhalites (VE). Methods The levels of NSE and sICAM-1 in serum and CSF were determined before and after treatment using ELISA in 58 patients with VE and 20 normal persons. Results The levels of NSE and sICAM-1 in serum before treatment were obviously higher than those of control group, and their differences were significant (P0.05). Conclusions NSE and sICAM-1 may contribute to pathologic course in infection of VE and the levels of NSE and sICAM-1 in serum and CSF may serve as markers of differential diagnosis and clinical significance.

Objective To evaluate the role of c-Jun N-terminal kinase (JNK) signaling pathway in isoflurane (ISO) preconditioning against cerebral ischemia/reperfusion (I/R) injury and investigate the relationship between JNK signaling pathway and apoptosis.Methods Global cerebral I/R models were made by 4-artery occlusion technique.Forty male SD rats of clean grade were divided into sham operation group (S group),I/R injury group (I/R group),SP600125 (an inhibitor of JNK signaling pathway) + I/R group (SP + I/R group),ISO preconditioning group (ISO group),and ISO preconditioning + SP600125 group (ISO + SP group) according to the random number table.Preconditioning protocol was successive inhalation of 15 g/L ISO for 5 days,1 h/d.I/R was induced at 24 hours after the end of preconditioning.Brain tissues were harvested at 72 hours later to take histomorphological examination by HE staining as well as detect apoptosis of hippocampal nerve cells by TUNEL method,expression of caspase-3 in hippocampal nerve cells by immuno-histochemistry,and expression of protein p-JNK in hippocampal tissues by Western blot.Results Compared with S group,brain injury score,apoptosis ratio of nerve cells,and expression of caspase-3 were significantly increased in the other groups (P ＜ 0.05).Moreover,p-JNK protein had a higher expression in IR group than in S group (P ＜ 0.05),but no significant difference was observed in SP + I/R group,ISO group,and ISO + SP group as compared with S group (P ＞ 0.05).Compared with I/R group,brain injury score,apoptosis ratio of nerve cells,expression of caspase-3,and expression of p-JNK protein were all declined in SP + I/R group,ISO group,and ISO + SP group (P ＜ 0.05).Moreover,brain injury score,apoptosis ratio of nerve cells,and expression of caspase-3 had further decline in ISO + SP group as compared with SP + I/R group and ISO group (P ＜ 0.05),but the difference in expression of p-JNK protein was insignificant among the three groups.Compared with SP + I/R group,no significant changes of each index were found in ISO group.Conclusion ISO preconditioning alleviates cerebral I/R injury through down-regulating expression of caspase-3 and inhibiting JNK signaling pathway.

OBJECTIVETo analyze a case with Angelman syndrome (AS) using single nucleotide polymorphism array (SNP array) and explore its genotype-phenotype correlation.

METHODSG-banded karyotyping and SNP array were performed on a child featuring congenital malformations, intellectual disability and developmental delay. Mendelian error checking based on the SNP information was used to delineate the parental origin of detected abnormality. Result of the SNP array was validated with fluorescence in situ hybridization (FISH).

RESULTSThe SNP array has detected a 6.053 Mb deletion at 15q11.2q13.1 (22,770,421- 28,823,722) which overlapped with the critical region of AS (type 1). The parents of the child showed no abnormal results for G-banded karyotyping, SNP array and FISH analysis, indicating a de novo origin of the deletion. Mendelian error checking based on the SNP information suggested that the 15q11.2q13.1 deletion was of maternal origin.

CONCLUSIONSNP array can accurately define the size, location and parental origin of chromosomal microdeletions, which may facilitate the diagnosis of AS due to 15q11q13 deletion and better understanding of its genotype-phenotype correlation.

Angelman Syndrome ; genetics ; Child ; Genotype ; Humans ; Karyotyping ; methods ; Male ; Phenotype ; Polymorphism, Single Nucleotide ; genetics

Objective To summarize the experiences of aneurysmorrhaphy for arteriovenous fistula aneurysms with acute thrombosis in hemodialysis patients.Methods There were 7 cases of arteriovenous fistula with acute thrombosis from Nov 2015 to Feb 2017 at our department of vascular surgery,Longhua Hospital.Results In all cases thrombosis was secondary to proximal stenosis or occlusion.The stenosis and occlusion were corrected with embolectomy and aneurysmorrhaphy.The proximal part of the cephalic vein was translocated to the basilic vein in 1 case,axillary vein in 2 cases;autologous vein graft in 1 case;resection of the occlusion,end-to-end anastomosis in 1 case;autogenous patch in 1 case.No perioperative complications occurred.The operation site was cannulated within one month after operation in all cases.Patients were followed up for 7 months to 23 months,all cases were patent.Conclusions Aneurysmorrhaphy is effective,reliable and safe for arteriovenous fistula aneurysms with acute thrombosis in hemodialysis patients.