AIM: To explore the properties of the acetylcholine(ACh)-sensitive potassium channel in type Ⅱ vestibular hair cells(VHCs Ⅱ) in mice saccular macula and the modulation effect of calcium ions.METHODS: Under the whole-cell patch mode,the pharmacology properties of ACh-sensitive potassium channel and the modulation of calcium ions on ACh-sensitive potassium channel were investigated.RESULTS: Following extracellular perfusion of ACh,VHCs Ⅱ displayed a slow and sustained outward current,which was sensitive to tetraethylammonium(TEA,5 mmol/L) and charybdotoxin(CTX,100 nmol/L),but not sensitive to 4-aminopyride(4-AP,15 ?mol/L).ACh-sensitive potassium current was inhibited by intracellular application of ethylene glycol-bis(B-aminoethylether)-N,N,N',N'-tetraacetic-acid(EGTA,5 mmol/L) and extracellular perfusion of Cd2+ and Ni2+,respectively.Intracellular application of heparin(8 g/L) failed to inhibit ACh-sensitive potassium current.CONCLUSION: Extracellular application of ACh activates the big conductance,calcium-dependent potassium current(BK) in VHCs Ⅱ of mice,which is potently modulated by extracellular Ca2+ ions.However,intracellular IP3-dependent Ca2+ ions release mechanism is not involved in the activation of the ACh-sensitive BK channel.

In view of the problems existing in medical image network teaching, such as redundant and rigid curriculum system, obsolete structure, untimely updates, lack of supervision system and poor stability of network security and so on, we explored how to improve the medical image network teaching from the fusion of traditional teaching, optimizing curriculum system, perfecting the medical imaging system of autonomous learning, encouraging online communication, creating a good atmosphere, increasing invest-ment in software development and multi-sectoral collaboration, in order to complete the transfer of knowl-edge more effectively, and promote the good development of medical imaging teaching.

The relationship between hypermethylation of CpG islands in the promoter regions of O6-methylguanine DNA methyltransferase (MGMT) genes and laryngeal squamous cell carcinoma was explored. Methylation-specific PCR and semi-quantitative RT-PCR were used to study the promoter methylation and mRNA expression of the MGMT gene in laryngeal carcinoma tissues, tissues adjacent to the tumor and normal laryngeal tissues. Hypermethylation of MGMT gene was detected in 16 samples of 46 (34.8%) laryngeal squamous cell carcinoma samples. However, the MGMT hypermethylation was not detected in all tissues adjacent to the tumors and normal tissues. No significant difference in MGMT gene hypermethylation was found in samples with different histological grades (chi2 = 3.130, P = 0.077) or in samples from patients with different TNM status (chi2 = 3.957, P = 0.138). No expression of MGMT mRNA was detected in all hypermethylated laryngeal carcinoma tissues. The expression of MGMT mRNA was detected in all unmethylated laryngeal carcinoma tissues, tissues adjacent to the tumors and normal tissues. It suggests that MGMT gene promoter hypermethylation is associated with MGMT gene transcription loss in laryngeal carcinoma tissues and possibly plays an important role in carcinogenesis of laryngeal tissues.
Carcinoma, Squamous Cell/*genetics ; CpG Islands/genetics ; DNA Methylation ; DNA Repair ; Laryngeal Neoplasms/*genetics ; O(6)-Methylguanine-DNA Methyltransferase/*genetics ; Polymerase Chain Reaction/methods ; Promoter Regions (Genetics)/*genetics

Dendrobium officinale is a kind of precious Chinese herbal medicine, which has good effects on the anti-aging, antineoplastic, and blood glucose reducing treatment. It has been listed in the top of “Nine Magic Herbs”, with significant cultural features of traditional Chinese medicine (TCM). Planting, processing, and cultural base construction of Dendrobium officinale in Hunan Province have obtained great achievements. Further combination of Dendrobium officinale with TCM culture will develop a series portable and convenient tourism products with the features of wide taking habits and large intended population. The planting of Dendrobium officinale should be integrated with ecofallow organically; the construction of science museums about Dendrobium officinale should be improved; the modern view on health maintenance “Health maintenance should focus on Yin nourishment” should be included in TCM cultural tourism in Hunan Province. All of these can improve the potential of tourism product development, meet the living requirements of modern people, and drive the development of tourist industry in Hunan Province.

Purpose/Significance A method is proposed for extracting data elements from electronic medical records(EMR)based on national standards,helping to achieve fine-grained sharing of EMR data.Method/Process The ALBERT,BILSTM and CRF models are used to perform sequence labeling on EMR,and a set of candidate data elements based on labeling results are generated.For any can-didate data elements,the contextual information is collected to form an enhanced key vector.Then the similarity between the vector and the standard vector is calculated to determine whether the candidate data element is valid.Result/Conclusion The F1 value is 90.32％,indicating the proposed method has a good performance.

Objective To study the composition of variant and point mutations of Norovirus GⅡ.4 in Guiyang regions.Methods From June to November 2010,cases information and fecal specimens were collected from guard-hospitals in Guiyang regions,who had caught the acute-gastroenteritis.Noroviruses in specimens were detected by a real-time reverse transcription polymerase chain reaction(real-time RT-PCR),and then partial genotyped norovirus-positive clinical samples (in random) were cloned and sequenced in VP1 gene code.Furthermore,the gene sequences were compared with the published variants at home and abroad of norovirus(GⅡ.4),including the phylogenetic analyses of genomes and variation of amino acids within individual sites.Results Those 267 specimens were GⅡ-norovirus-positive(62.68％) in 426 clinical samples.There were nine GⅡ.4-norovirus-positive VP1 gene-sequences available,and two subtype-norovirus variants (GⅡ.4 2008a and G Ⅱ.4 2008b variant) were epidemic in 2010,Guizhou province.The homology between and in subgroups were 95.90％-96.72％ and 99.45％-100％.Two amino acids within individual sites were apt to mutate.Conclusion Norovirus GⅡ genotype were predominant in summer and fall acute gastroenteritis in 2010 for Guiyang regions,and the variants were diversity.

Objectives Explore the experiences of professor Li Shunmin in treating chronic kidney diseases (CKD) according to spleen and kidney theory.Methods Information of medical records was acquired from Shenzhen TCM hospital information management department. It included the records from Jan, 2014 to Mar, 2016. Access database was established and SQL was used for data processing. Cytoscape 2.8 software was used to visualize the results and analyze the treatment characteristics in CKD.Results ProfessorLi used herbs of nourishing spleen and kidney to treat CKD. The herbs included Astragalus membranaceus, Rhizoma Dioscoreae, Rehmannia glutinosa and Gordon Euryale. The rules of treatment included invigorating spleen and kidney, and regulating liver and lung. The characteristics of using herbs included combination of cooling and warming herbs, bitter and pungent herbs, and sweet herbs for CKD.Conclusions Data mining could help to discover the rules of Li Shunmin in treating CKD. The results confirmed the academic attitude of treating CKD on spleen and kidney. It provided ideas and direction for CKD treatment.

It has been a general trend to open the 5+3 medical personnel training mode of radiology in China.But at present,there are still a lot of problems during the process,such as,lack of training plan,unified teaching materials and examination index for reference,lack of learning consciousness and self-discipline among the students who participate in the standardization training,lack of adequate teaching resources in the standardization training base,and so on.In order to cultivate qualified and high-quality radiologists,this paper discussed the ways of forming a new set mode of 5+3 medical personnel training in the radiology department through exploration and practice,including strengthening teachers' construction and building a professional teachers troop,reforming the teaching method of standardization training,improving the teaching base infrastructure,establishing and perfecting the assessment evaluation mechanism.

Objective We tried to observe the effects of Chinese herb fumigation and washing combined with electromyographic biofeedback on stroke patients with muscle cramp on limbs.Methods 84 patients who had muscle cramp on limbs were selected and divided into two groups randomly,namely the intervention group and the control group and both groups had 42 stroke patients.The intervention group was treated with routine treatment,Chinese herbal fumigation and washing and electromyographic biofeedback treatment.The control group received only routine treatment and electromyographic biofeedback treatment.We had compared and analyzed the Ashworth Spasticity Scale and the Daily Life Abihty Score (Barthel index) of the two groups at the time of the pre therapy,two weeks after the treatment and at the end of the treatment(after four weeks),respectively.Results There were no significant differences between the two groups in Barthel index,however,there were significant differences in Ashworth Spasticity Scale scores between the two groups four weeks after treatment,t=-3.84,P ＜ 0.05.Conclusions The effect of Chinese herb fumigation and washing combining with the electromyo-graphic biofeedback treatment is better than usage of the electromyographic biofeedback treatment alone on stroke patients with muscle cramp on limbs.

OBJECTIVE To investigate the prevalence of nasal colonization among health care workers(HCWs).METHODS Nasal swabs from 93 ICU workers and 98 other clinical workers were cultured and isolated and the tests of antibiotic susceptibility were performed by using paper diffusion method.RESULTS In total,214 isolates of 8 species from 191 health care workers were recovered,of which 187 isolates were coagulase-negative Staphylococcus(CNS)(the carriage rate of 93.71%) and 8 isolates were Staphylococcus aureus(the carriage rate of 4.19%).While the total Gram-negative bacteria carriage rate was 14.14%(27 isolates).The most frequent CNS species were S.epidermidis and S.haemolyticus.The antibiotic susceptibility profiles of S.aureus and CNS differed sharply: all 8 S.aureus strains were resistant to penicillin but were fully susceptible to oxacillin,in contrast,most of CNS were resistant to both penicillin and oxacillin.The carriage rate of CNS(60.2%)and Gram-negative bacteria(26.9%)in HCWs of ICU were higher than other HCWs(P