The authors have determined the effects of 261-B_2-3 and C_(12)b_3-2, extracts two strains of Alternaria alternata, on the nucleolus organizer regions (NORs) activity and the progression of cell proliferating cycle of human peripheral lymphocytes. The methods used in the experiment were selective silver staining of human NORs and cytophotometry. The result showed that the number of chromosomes bearing Ag-NORs was meaningly decreased when the cells were treated with different concentrations of 261-B_2-3 and C_(12)-b_3-2 extracts. The difference was quite significant when the data were compared with those of the control (P

OBJECTIVE:To study the pharmacoeconomic effectiveness of three antihyperlipoidemics METHODS:According to literature reports,158 patients with hyperlipoidemia were selected These patients were randomly divided into three groups which received zhibituo,pravastatin and simvastatin respectively The results were evaluated with pharmacoeconomic analysis RESULTS & CONCLUSION:The cost of zhibituo is minimal

OBJECTIVE:To optimize the formulation and preparation technic of azathioprine liposomes and evaluation the quality.METHODS:The liposomes were prepared by a film-vibration technique.The optimal formulation and preparation technic were selected by means of an orthogonal design test.The entrapment ratio of liposomes was studied by titrimetric method.The size distribution and appearance of liposomes were observed under microscope.RESULTS:The entrapment ratio of azathioprine liposomes was49.19%.The liposomes consisted of spherical or similar to spherical multilamellar vesicles with78.17%of particles in the liposomes smaller than2.4?m.CONCLUSION:The selected formulation and preparation technic of azathioprine liposomes are rational and stable.

The cultured human esophageal cancer cell line——Eca109 cells was incubated with the scorpion venom crude (SVC) collected from Buthus Martensii Karshiin Henan Province. The growth inhibition and cytotoxicity of SVC on Eca109 cells were detected. The results showed that Eca109 cell growth was inhibited by SVC. while Eca 109 cells were incubated with SVC for 24, 48, 72hrs. The rates of inhibition were 35.6%, 39.5, 36.9% respectively, the concention of SVC used was 0.017?g/ml. The mitochondrial dehydrogenase of Eca109 cells were also inhibited by SVC, Which had the cytotoxic effect on Eca109 cells. When the concentrations of SVC were 0.017?g/ml, 0.034?g/ml, 0.085?g/ml, the cytotoxicity were 63%, 56% and 59%, respectively. The effect and mechanism of cytotoxicity of SVC on the tumor cells are worth further studies.

Human fetal esophageal epithelial tissue were cultured in vitro and treatedwith mycotoxins of Alternaria alternata (AME or AOH) for 4 h. The genomic DNAwere extracted from these tissues. Genomic DNA was isolated from normal human fetalesophageal epithelium (as blank control), DNA from malignant tissue and its adjacentnormal mucosa was obtained from esophagectomy patients. DNA was amplified with PCRreaction, using genomic DNA as templet. The PCR products was a 104bp fragment from which the 12 codon of c-Ha-ras gene was contained. The excition point of restriction en-zyme Hpe Ⅱ was located in this fragment. The PCR amplified 104bp fragment was diges-ted by Hpa Ⅱ and analysed by agarose gel electrophoresis. The results showed that the104bp fragment amplified from genomic DNA of blank control and esophagectomy patientcould be digested by Hpa Ⅱ ; but that from genomic DNA of human fetal esophagealepithelium treated by AME or AOH could not. These results indicated that a mutationhad taken place at 12-codon of c-Ha-ras gene after it was treated by AME, AOH for ashort time. The mutation of Ha-ras gene might be the early event during esophageal car-cinogenesis. The effect of AME and AOH during the onset of esophageal cancer and themolecular machanisms of the effect were worth of further study.

OBJECTIVE:To compare the pharmacoeconomic effectiveness of three antiviral agents for herpes zoster ME_THODS:101 patients with herpes zoster were divided into three groups who received acyclovir injection,valaciclovir tablet and ribavirin injection,respectively Evaluation was carried out with cost-effectiveness analysis RESULTS:The cost-effectiveness ratios of three groups were $1 288 2,$863 0 and $949 8 CONCLUSION:Valaciclovir was the best antiviral agents for herpes zoster

Objective To evaluate the safety and feasibility of portal CO 2-DSA with fine needle splenic puncture. Methods The splenic tails of seven adult white rabbits were exteriorized by laparotomy, and followed by a 25 gauge fine needle inserting about 1.0 cm into the splenic parenchyma. Portal CO 2-DSA was performed (2.0 ml/s, 10ml) and the images were evaluated. After removal of the needle, the puncture site was observed for bleeding till coagulation occurred. The spleen were taken for gross and histological examination. Results All the CO 2-DSA clearly showed the portal trunk with intrahepatic branches above 3～4 orders, the main splenic vein, and the main mesenteric veins with parts of its branches. CO 2 disappeared from the intrahepatic portal vein over 2～3 minutes. In one animal, the left renal vein and the inferior vena cava were also displayed by CO 2 through communication between splenic vein and renal vein. After removal of the needle, there was small amount of bleeding at the puncture site which ceased spontaneously over 3～5 minutes. In all animals, no extravasation of CO 2 at the puncture site, no subcapsular dissection or intrasplenic hematoma was observed. Microscopically, the splenic capsule appeared intact and there was no evidence of subcapsular hematoma formation.Conclusions Portal CO 2-DSA with fine needle splenic puncture is feasible, safe and efficient. In normal adult rabbit, CO 2 may help to visualize the left renal vein and inferior vena cava through communication between splenic and renal vein.

Objective To study the feasibility and safety of the diagnostic coronary angiography by transradial approach with modified 5 F universial catheter.Methods Retrospective analysis was performed in 268 randomly chosen patients undergoing diagnostic coronary angiography by transradial approach,either with 5 F universial catheter(universial group,n=133) or 5 F modified universial catheter(modified group,n=135).The success rate and the duration of operation and fluoroscopy,the rate of error entry into branch vessel between the 2 groups were compared.Results The success rate for angiography was 87.2%(116/133) in the universal group and 97.0%(131/135) in the improved group(P

Objective To study the antibacterial substances produced by Musca domestica and heattreatment and electrophoresis isolation of the substances. Methods After the 3rd instar larval of Musca domestica being induced for 48 hours by pricking,the hemolymph was collected and bathed at 100 ℃ for 0,0.5,1,3,5 min. Antibacterial substances of Musca domestica was isolated by electrophoresis with acide polyacrylamide gel using 5,10,20,30 ?l doses. Results 20 ?l was the optimum dose both for bathed and non-bathed hemolymph. Water bath at 100 ℃ for 1 min was the optimum bath time for hemolymph. Comparing induced hemolymph with non-induced hemolymph,the protein of the induced hemolymph was increased in the number and content. After 100 ℃ bathed for 1 min,it showed that the bonds of hemolymph increased both in quantities and contents. Conclusion Optimum treatment is 100 ℃ bath for 1 min and 20 ?l of adding dose. Pricking inducing can produce new proteins in hemolymph, which are the new induced antibacterial substances.

Purpose:To investigate the effect of antineoplastic polypeptide from Buthus martensii venom (APBMV) on the cultured human promyelocytic leukemia cells HL 60 and hepatoma cell line SMMC 7721 and hepatoma H 22 bearing mice.Methods:MTT colorimetric method, growth inhibiting test and colony formation assay were used in the in vitro test. H 22 bearing mice were applied in the in vivo experiment. Through measuring tumor growth inhibitory rate(IR),white blood cell (WBC) number and spleen index (SI) ,we explored the influence of APBMV on H 22 bearing mice.Results:APBMV possessed stronger cytotoxicity on HL 60 cells and SMMC 7721 cells, and IC 50 was 10.74 ?g/ml and 11.33 ?g/ml , respectively. APBMV could dramatically inhibit their growths. There were obvious dosage response correlations. The IC 50 of HL 60 and SMMC 7721 at 24h, 48h and 96h were 19.41 ?g/ml, 9.90 ?g/ml, 11.41 ?g/ml and 15.87 ?g/ml, 13.05 ?g/ml, 8.70 ?g/ml, respectively. When the concentration of APBMV exceeded 8 ?g/ml, the colony formation rate of SMMC 7721 cells decreased dramatically ( P 0.05).Tumor growth of H 22 bearing mice was markedly inhibited by APBMV,the growth inhibiting rate was reached 40.30% ( P