OBJECTIVE To determine the distribution of pathogens of bacterial infection in respiratory intensive care unit (RICU) to provide reference for the prevention of hospital infection. METHODS To collect specimens of the patients received endotracheal intubation from Jan 2006 to Dec 2006 in our RICU,to identify pathogens and drug sensitivity test. RESULTS We isolated 105 pathogenic bacteria from 626 specimens of the 58 patients,the G-bacilli accounted for 64.76%,G+ cocci accounted for 20.95%,fungi accounted for 12.38%; Acinetobacter baumannii was one of the main G-bacilli accounted for 19.05%. Staphylococcus aureus of G+ cocci for 9.52%,and Candida albicans of fungi for 7.62%; from 105 pathogens,65 from the respiratory tract,15 from the for urinary tract,eight from a catheter tube,six from the digestive tract,five from the blood,four from the various drainage tubes and two from the incision secretions. CONCLUSIONS Hospital infection pathogens exist in the respiratory tract infection in hospitalized patients of respiratory ICU with risk factors of age,underlying diseases,time in the intensive care unit,ventilator time and the use of invasive procedures in a certain relationship.

Objective To explore the value of laparoscopic ovarian drilling in the treatment of polycystic ovarian syndrome with infertility.Methods The clinical data of 51 patients with polycystic ovarian syndrome with infertility undergone laparoscopic ovarian drilling were reviewed.The Serum concentrations of follicle-stimulating hormone(FSH),luteinizing hormone(LH),androgen(T),and estradiol(E2) were measured before and after the operation in all of the cases.Meanwhile,ovarian follicular development and pregnancy rate were observed during follow-up.Results Significantly decreased levels of FSH,LH,LH /FSH,and T were observed after the laparoscopic ovarian drilling in the patients(P

Objective To investigate the mechanisms of endothelial cell injury in women with pregnancy induced hypertension (PIH). Methods Human umbilical endothelial cells were cultured in the serum from 10 women with severe PIH and 10 normal pregnant women, and co-cultured with neutrophils or not for 48hrs. The morphology and cell cycles were studied. Results Without neutrophils, no apoptotic cells were observed, while neutrophils were added, apoptotic cells were obviously detected. The percentage of apoptotic cells in PIH was more than that in non-PIH. The percentage of cells in G 2/M phase decreased in all the groups, but there were no significant differences among them. Conclusions Endothelial cells were injured indirectly in PIH patients. Activated neutrophils induced apoptosis might be one of the answers.

Objective To study the probability of screening the high risk people of hypercholestrolemia by cholestrol level in cord blood. Methods Cord venous blood were collected in 1000 newborns and the cholestrol and glucose level were measured. Maternal diet, birth weight and delivery time were recorded. Results The mean level of cholestrol in cord blood was (64.86?17.25) mg% in full term newborns, 42 cases were higher than 100 mg%(4.2%). No relationship was shown between the cholestrol level in cord blood and that in the mother blood or maternal ,diet or delivery time. The glucose level in cord blood was affected by what the mother eating during the 3 hours before delivery. Conclusions The cholestrol level in cord blood of full term newborns may be used as the earliest screening method for hypercholestrolemia people.

0.05). Conclusions It is suggested that ANCA may be contribute to the pathogenesis of PIH. The exactly role of ANCA in the pathogenesis of PIH remains unknown.

0. 05). No significant difference was found in maternal and neonatal complications between the ANCA( + ) and ANCA(－) subgroups in S-PE subjects. But those 4 cases who developed renal function insufficiency patients were ANCA( + ). Conclusions ANCA might be associated with renal diseases in preeclampsia women, and further studies is required to determine whether ANCA is involved with the pathogenesis of preeclampsia.

AIM To study the effects of parathyroid hormone(PTH) on intracellular calcium concentration([Ca 2+ ] i), hypertrophy and apoptosis in rat cardiomyocytes. METHODS Neonatal rat cardiomyocyte hypertrophic response was assayed by measuring cell surface area and protein content. [Ca 2+ ] i was measured by confocal microscope using Fluo 3/AM as fluorescent indicator.Apoptosis was determined by flow cytometer and electron microscope. RESULTS After 7 d exposure to PTH 1～34 of 0 01 and 0 1 ?mol?L -1 ,[Ca 2+ ] i,surface area and protein content,and apoptosis rate(APO) of cardiomyocytes were significantly increased,especially in high concentration groups. While application of both nifedipine and PTH 1～34 significantly decreased lose indicators, they were still markedly higher than normal. CONCLUSIONS PTH 1～34 dose dependently induces hypertrophy and apoptosis of cardiomyocyte,and this is attributed able to augmented entry of calcium into cell via voltage dependent Ca 2+ channel.

Objective To investigate clinical features and drug resistance of neonate septicemia caused by Streptococcus agalac-tiae in the hospital and to provide clinical guidance for treatment.Methods 42 Streptococcus agalactiae isolates in the hospital were identified and their department distribution as well as drug susceptibility was determined by using automatic VITEK-2 system and criteria released by CLSI in 2012.Results The number of cases in preterm neonatal intensive care unit,full-term neonatal intensive care unit,pediatric intensive care unit and extremely preterm neonatal intensive care unit were 3,26,7 and 6,respectively.The drug resistance rates of the isolated Streptococcus agalactiae to ampicillin,erythromycin,clindamycin,quinupristin/dalfopristin,linezolid, penicillin,tetracycline,tigecycline,vancomycin and levofloxacin were 0.0%,69.0%,71.4%,0.0%,0.0%,0.0%,74.4%,0.0%, 0.0% and 38.1%,respectively.Conclusion The antimicrobial resistance rates of Streptococcus agalactiae which caused neonatal septicemia in our hospital are relatively high.Ampicillin and penicillin are recomended in treatment for neonate septicemia caused by Streptococcus agalactiae .

Objective To diagnose retinal detachment with International Classification of Diseases10 (ICD-10) and to analyze the surgical modalities of vitrectomy for the disease.Methods The clinical data of 1 648 hospitalized patients with retinal detachment undergoing vitrectomy in our hospital in 2013 were retrospectively analyzed.Results The ICD etiological classification showed that primary retinal detachment accounted for 50.18％ (827/1 648),tractional retinal detachment accounted for 32.52％ (536/1 648),exudative retinal detachment accounted for 0.61％ (10/1 648)and other types accounted for 16.69％ (275/ 1 648).The top 5 common etiological diagnoses were simple rhegrnatogenous retinal detachment (25.73％,424/1 648),diabetic tractional retinal detachment (12.62％,208/1 648),traumatic retinal detachment (12.08％,199/1 648),other primary retinal detachment (11.23％,185/1 648) and retinal detachment after intraocular surgery (10.62％,175/1 648).The top 4 modalities of vitrectomy were combined silicone oil implanting surgery (54.73％,902/1 648),basic vitrectomy (21.97％,362/1 648),combined silicone oil and lenticular surgery (14.38％,237/1 648) and combined lenticular surgery (5.58％,92/1 648).Conclusions Modern vitrectomy is used mainly for the treatment of primary retinal detachment and the treatment of retinal detachment related to diabetes,ocular trauma and intraocular surgeries.The complexity of various retinal detachment diseases requires different surgical modalities of vitrectomy for treatment.

Objective To study the incidence of pelvic adhesions in endometriosis(EM) and the relationship between pelvic adhesions and pain symptoms. Methods The incidence of pelvic adhesions, dysmenorrhea, chronic pelvic pain, dyspareunia, dyschizia in 480 patients with EM were studied retrospectively to evaluate the correlation between pelvic adhesions and the degree of pain symptoms. In accordance with the revised American Fertility Society classification (r-AFS), it was observed that 155 cases were in Stage Ⅰ,33 cases were in stage Ⅱ,108 cases were in stage Ⅲ and 184 were cases in stage Ⅳ. Results (1) Among 480 cases with EM, 72.3% (347/480) of patients have pelvic adhesions simultaneously, of which 25.2% (39/155) patients were in Stage Ⅰ, 78.8% (26/33) patients were in Stage Ⅱ, 90.7% (98/108) patients were in Stage Ⅲ and 100.0% (184/184) patients were in Stage Ⅳ. It was found that positive correlation existed between the degree of pelvic adhesions and clinical staging of EM(rs=0.870,P<0.01).(2) 61.0%(293/480) of patients presented dysmenorrhoea, of which the percentages of mild, moderate and severe dysmenorrhea were 52.2%(153/293), 26.6%(78/293), 21.2% (62/293), respectively. The rate of patients presenting chronic pelvic pain (CPP), dyspareunia and dyschezia were 23.8% (114/480), 15.4% (74/480) and 7.1% (34/480), respectively. (3) Ovarian adhesions was positively correlated with dysmenorrhea and CPP(rs=0.367 and 0.267,P<0.01). Adhesion of the bottom and posterior wall of uterus was positively correlated with dysmenorrhea and CPP (rs=0.336, 0.164,P<0.01); adhesions of fallopian tubes were positively correlated with dysmenorrhea, CPP and dyschezia (rs=0.283, 0.225 and 0.159, P<0.01). Adhesions of rectum was positively correlated with dysmenorrhea (rs=0.101,P<0.05). In addition to dyspareunia, the degree of pelvic adhesions was positively correlated with dysmenorrhea, CPP and dyschezia (rs=0.470, 0.273, 0.132, P<0.01). Conclusion Pelvic adhesions are characteristic lesions of endometriosis, the site and degree pelvic adhesions are closely correlated with pain symptoms.