OBJECTIVE:To evaluate the effect of single decoction vs.mingled decoction on the content of Baicalin in Radix Scutellariae.METHOD:The separation of samples was performed on Agilent C18 column(250 mm?4.6 mm,5 ?m)with a column at room temperature.The mobile phase consisted of 0.5% formic acid-methano and 0.5% formic acid solution(55∶45)with flow rate of 1.0 mL?min-1 and detection wavelength of 274 nm.RESULTS:The linear range of Baicalin was 0.258 5～2.326 5 ?g(r=0.999 9)with an average recovery of 97.8%(RSD=1.1%,n=9).CONCLUSION:The content of Baicalin in Radix Scutellariae decocted alone is higher than in the mixture of all the single decoctions,and significantly higher than in Radix Scutellariae decocted by mingled decoction,all showing significant differences.

Objective To investigate the effect of health education in the community of high risk group of pressure sore , and to provide a reference for the formulation of community health education strategies .Methods 148 subjects with NORTON score discharge assessment results less than or equal to 14 points were randomly divided into observation group and control group with 74 cases in each group.The control group received routine discharge instructions.The observation group in the conventional discharge guidance on the implementation of the home, regular and telephone guide.The self-made pressure ulcer prevention knowledge questionnaire were com-pared between the two groups at the time of discharge and intervention for caregivers to grasp the situation of pressure ulcer prevention knowledge after 3 months;The incidence rate of discharged patients in the two groups after the comparison of pressure ulcer were com-pared.Results After the intervention, the observation group significantly increased the pressure ulcer prevention, which was signifi-cantly higher than before the intervention and the control group after intervention (all p<0.05);In the observation group was discharged after 3 months, 6 months and 9 months were no bedsore.Control group 3 months after discharge pressure sore the incidence rate was 8. 11%.6 months after discharge pressure ulcer incidence rate was 16.22%.9 months after discharge pressure ulcer incidence was 22. 97%.The observation group of 3 months, 6 months, 9 months after hospital discharge rate of pressure ulcer was significantly lower than that of control group (all p<0.05).Conclusion Health education is helpful to improve caregivers knowledge related to pressure ulcer prevention, reduce the risk of high-risk population community bedsore, and is worthy of popularization and application.

Objective To investigate if Carroll Upper Extremities Functional Test (UEFT) is a better rating scale for evaluating fine mo-tor function in children with cerebral palsy. Methods From January, 2011 to January, 2014, 104 children with cerebral palsy were divided in-to non-hemiplegic palsy group (n=76) and hemiplegic palsy group (n=28), they were assessed by two certified valuers in one week. Their fine motor function was evaluated with UEFT and the Gesell Developmental Scale. The scores of UEFT were compared respectively with the developmental age (DA) and developmental quotient (DQ) in fine motor domain of Gesell Developmental Scale. At the same time, the UEFT score of the affected limb was compared with of the unaffected limb in children with hemiplegic palsy. Results In the non-hemiplegic palsy group, the UEFT score strongly correlated with DA (r=0.752, P<0.001) and moderately correlated with DQ (r=0.456, P<0.001). In the hemiplegic palsy group, the UEFT score of the unaffected limb strongly correlated with DA (r=0.722, P<0.001), and a moderate correlation was found in the affected limb (r=0.591, P<0.001). The UEFT scores of both sides did not correlated with DQ (r<0.186, P>0.05). The UEFT score of unaffected limb was significantly higher than that of the affected limb (Z=-4.708, P<0.001). Conclusion Both of UEFT and the Ge-sell Developmental Scale could be used clinically in assessment of fine motor function in children with cerebral palsy. The UEFT was better in evaluating children with hemiplegic palsy.

Endothelial cells can be activated, and develop dysfunction and damage under the pathological state. Endothelial dysfunction plays a key role in the processes of cardio-cerebrovascular diseases, and can be detected in peripheral blood. The concentration of von Willebrand factor (vWF) predicts the risk of coronary heart disease and stroke; the concentration of soluble thrombomodulin is associated with the prognoses of coronary heart disease and stroke; soluble intercellular ashesion rnolecule-1 (ICAM-1) predicts the risks of carotid athero-sclerosis, coronary heart disease, peripheral arterial disease, and diabetes mellitus; the levels of plasma soluble E-selectin in patients with carotid atherosclerosis, coronary artery disease, and diabetes mellitus are increased; the increased endothelin-1 levels predicts the elevated risk of congestive heart failure. The injection of endothelial progenitor cells is promising to become a novel approach for the treatment of cardio-cerebrovascular diseases.

Objective To investigate the composition and drug resistance of pathogenic bacteria of nongonococcal urethritis in men,in order to give some references for clinical treatment.Methods The pathogens were cultured by Urethral secretions of nongonococcal urethritis in men and drug susceptibility test followed.Results Coagulase negative staphylococcus and mycoplasma urealytium were major pathogens of nongonococcal urethritis in men;The constituent ratio of meticillin resistance staphylococcus was above 40%.There was significant difference that the constituent ratio of meticillin resistance coagulase negative staphylococcus compared with specimen in the same period.Meticillin susceptible coagulase negative staphylococcus and meticillin susceptible Staphylococcus aureus were sensitive to cephalosporins and quinolones,excluding penicillins.The antimicrobial susceptibility of meticillin resistance staphylococcus was just opposite completely;Mycoplasma urealytium and Mycoplasma hominis were high sensitivity to josamycin、doxycyline and minocycline.Conclusion The colonization bacterium of nongonococcal urethritis in men are primarily consist of coagulase negative staphylococcus and mycoplasma urealytium.It is worthy of further researching correlation between coagulase negative staphylococcus and nongonococcal urethritis,it also suggested that antibiotics on should be rationally choosed on therapy of nongonococcal urethritis.

Objective To explore the clinical effect of the two different administration style of insulin pump to treat type 2 diabetes.Methods 118 patients with type 2 diabetes were selected and randomly divided into the experimental group,which 59 cases of the patients were treated with insulin pump (CSII group) and the control group,which 59 cases of the patients with subcutaneous injecting of insulin.The treat ment effects were observed.Results The blood glucose level of the two groups of patients was significantly decreased after treatment,the CSII group spend shorter time [(4.5 ± 1.3 ) d vs (7.2 ± 2.3 ) d],used a significantly less dose of insulin and has a lower incidence of hypoglycemia than the MDII group in the course of decreasing the blood glucose to aimed level(all P ＜0.05 ).Conclusion Insulin pump is safe and more effective than subcutaneous injecting insulin in treating type 2 diabetes,and has no significant adverse reactions,worthy of clinical application.

ObjectiveTo observe the clinical efficacy and application value of preoperative application of hydrochloride tirofiban in patients with acute coronary syndrome(ACS) by percutaneous coronary intervention (PCI).Methods60 ACS patients underwent PIC surgery were randomly divided into observation group and control group.All patients underwent preoperative and postoperative regular routine anti-angina and acute myocardial infarction treatment,the observation group was given the hydrochloride tirofiban in 1h before surgery of emergency PCI,the control group received conventional therapy.The bleeding and thrombocytopenia occurrence during hospitalization of the two groups were observed,before surgery and 1 month after surgery the cardiac function of the two groups were compared,1 month after surgery the occurrence of adverse cardiovascular events were recorded.ResultsThe bleeding incidence of observation group was 6.7％,the control group was 23.3％,the difference between two groups was significant ( x2 =15.594,P ＜ 0.01 ) ; The thrombocytopenia occurrence of observation group was 3.3％,the control group was 10.0％,the difference between two groups was significant( x2 =13.366,P ＜ 0.01 ).1 month after surgery the LVEF,CO and E/A of the two groups increased significantly,compared with the prior-treatment,the difference was significant (P ＜ 0.05 ),and the difference was significant between the two groups( P ＜ 0.05 ) ;The occurrence of adverse cardiovascular events of observation group was 33.3％,the control group was 66.7％,the difference between two groups was significant(x2 =8.924,P ＜ 0.01 ).Conclusion The hydrochloride tirofiban is a safe and effective anti-platelet drugs.Preoperative application of hydrochloride tirofiban in patients with ACS by PCI can reduce the occurrence of cardiovascular events.

Objective To study the different influences of Xiaobanxiatang superfines (XBTs) made of Chinese medicinal materials and traditional decoction (XBTd) on gastrin. Method The model of mice were made with cisplatin. To observe the effect of the different forms of XBT on gastrin in serum and small intestine. Results After giving medicine, XBTd and XBTs remarkably increased the gastrin in serum and small intestine (P

Ten cats lightly anesthetized and D-tubocurarine immobilized were employed.The responses of a same unit to the train stimuli of square wave applied to the saphenous nerve,the greater splanchic nerve,and the two nerves simultaneously were observed.Among the 80 units recorded on the 10 cats,43 went through all the experimental procedures,33 out of the 43 could be activated by splanchic inputs.Convergence of the splanchic and saphenous inputs occurred to 26(79％) out of the 33.These 26 units could respond to the noxious stimuli on the saphenous and greater splanchic nerves in turn or simultaneously.Furthermore,they could be divided into 3 types;15 units showed similar pattern and duration of discharge only when the greater splanchic nerve was stimulated,6 showed similar discharge only when the saphenous nerve was stimulated,and 5 showed smaller responses when either of the two nerves was stimulated.When the interval between stimulations was shortened to 5 seconds,37(86％) out of the 43 units showed a decrease in discharge,which was apparently an after-inhibitory effect.The results indicate that the inputs from visceral and somatic C or A-delta afferent fibers can converge onto the units of the dorsal hippocampal CA1 area,and evoke blockage and inhibition of these units.

Objective: To determine the adjuvant activity of recombinant human HSP70D protein. Methods: Dendritic cells were cultured in vitro , rhHSP70D was added into the culture medium, the cytokine secretion and maturation of dendritic cells(DC) stimulated by rhHSP70D were measured; C57BL/6 mice were immunized with rhHSP70D OVA 257 264 complexs reconstituted in vitro , and the protective antigen specific immune response against tumor challenge were evaluated in the immunized mice. Results: rhHSP70D stimulated human DC to secrete IL 1?, IL 12 p70 and TNF ?, up regulated the HLA DR,CD86 and CD40 expression on DC and improved the antigen presentation function of DC; After immunization with the hybrid of rhHSP70D and OVA 257 264 , the mice were able to resist the challenge of E. G7 OVA, which was a tumor cell line expressing model antigen OVA. Conclusion: rhHSP70D can induce maturation of DC, immunization of rhHSP70D peptide complexs can induce antigen specific immune response, suggesting that rhHSP70D may be a new adjuvant in immunotherapy of the cancer and infection diseases.