OBJECTIVE:To establish a method for the content determination of sinomenine in Qihuang capsule. METHODS:HPLC was performed on the column of welch C18-AQ with mobile phase of methanol-phosphate buffer(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 264 nm,column temperature was 30 ℃ and volume injection was 10 μl. RESULTS:The linear range of sinomenine was 0.200 3-10.016 0 μg(r=0.999 8),RSDs of precision,stability and reproducibility tests were lower than 1.0%,recovery was 98.80%-100.94%(RSD=0.79%,n=6). CONCLUSIONS:The method is simple,accurate and re-producible,and can be used for the content determination of sinomenine in Qihuang capsule.

Objective To analyze and identify the main chemical constituents of extract from Cordyceps hawkesii by using method of ultra performance liquid chromatography-qudropole time-of-flight mass spectrometry (UPLC-Q-TOF-MS).Methods UPLC condition was as the following: Agilent SB-C18 (50 mm×4.6 mm, 1.8 μm) column with gradient elution consisting of 0.1 % formic acid in water and acetonitrile, at a flow rate of 0.25 mL/min.The column temperature was set at 30℃.MS analysis was based on information associated mode (IDA) that positive and negative ions were respectively collected.Results Mass spectrometry combined with "Formula Finder" of PeakView software and database, a total 26 compounds were identified as alkaloids, glycosides, nucleosides, and amino acids.9 of them had never been reported in Cordyceps hawkesii, and 2 of them were known.Conclusion The method is accurate, reliable and efficient for rapid identification of compounds in Cordyceps hawkesii.The results provide references for the development and utilization of Cordyceps hawkesii, as well as finding out its pharmacodynamic material basis.