For the purpose of opening up the future of more widespread application of cord blood,and also for the purpose of setting up the, bank of hemopoietic stem sells in cord blood,an effective method of cryopreserving the cord blood was approached, indicating, that it was appropriate that the time of cryopreserving the hemopoietic stem cells in cord blood at 4℃ should be 2-3 days (the storage of hemopoietic stem cells in cord blood with liquid nitrogen was an effective method of cryopreserving the hemopoietic stem cells in cord blood,with high recovery rate,and longterm cryopreservation without any change in the recovery rate;the effect of storing the hemopoietic stem cells in freezer at-80℃ was similar to that of cryopreserving them with liquid nitrogen,and the former was simple,e-conomic, convenient and practical, and needed no any programmed temperature-dropping apparatus .At the same time, it is convenient to using the stem cells in large scale and to setting up the bank of them. Preserving the whole cord blood can avoid the loss of hemopoietic stem cells in cord blood during the centrifuging process,with the effect of preservation being unchanged.

ObjectiveTo explore the curative effect about treatment acute coronry syndrome(ACS) by song ling xue mai kang drug used.Methods110 patients with ACS were randomly distributed into treatment group and control group,treatment group included 56 patients,control group included 54 patients,therapy of treatment group added song ling xue mai kang drug base on general treatmnet,but therapy of control group was only general treatment.It was time for four weeks.All patients were examinated blood lipid and C-reactive protein level(CRP) and liver kideny function end of four weeks,and follow-up cardiovascular ischemic event ( reangina attack,remyocardial infarction,heart failure,cardiol vascular death).Results C-reactive protein level of treatment group was less than its of control group,it was significant by statistics test( P ＜0.01 ).It was significant different about cardiovascular ischemic event between treatment group and control group( P ＜ 0.01 ).ConclusionIt was beneficial to patient of acute coronary syndrome about uesd song ling xue mai kang drug,it could reduce inflammatory reactive to happen in ACS,and reduce badness event of cradiolvascular.

Objective To study the image features and clinical significance of 3-dimensional (3-D)and multiplanar reconstruction with spiral CT(SCT) in cervical spinal fractures.MethodPlain film radiography and spiral CT scanning were taken for 26 cases of cervical spinal fractures.The type of fractures, diagnositic successful rate, parameter of scanning and late stage managing methods were investigated retrospectively to find out the effective difference between the diagnosis of plain X-ray and SCT. The imagin location of fractures,sequence of cenrum, heighth of centrum, fracture line, auxillary structure and rotation/dislocation of the fractures were emphasized to evaluate the advantages of 3-D imaging.ResultIn the 26 cases, there were 4 Jefferson fractures, (in which 1 combined with articular dislocations), 17 C2 spinous process fractures, (in which 1 had failure of plain X-ray diagnostic), 1 Hangman fracture, 2 C1～2 imbalance, (in which 1 had failure of plain X-ray diagnostic).There were 2 cases of axoid longitudinal fractures without obvious dislocation. The diagnositic successful rate were 100% in SCT group, while 65% in X-ray group in which 6 cases had diagnostic failure and 3 cases had fracture mis-diagnozing as dislocation.The anatomical structure of upper cervical spine was complicated and was significantly different from other cervical spine constructions. Spiral CT could reveal high resolution images of the upper cervical spine, morphosis of vertibral canal, dislocation information of fracture mass and its relation with neighbouring tissues. Especially in C1 burst fracture,C2 dens fracture and C1～2 dislocation, SCT could provide clear 3-D information of the size of collateral mass and the dislocation degree, furthermore, images could be rotated freely to observe the fracture in any proper section. That provides important information for the decision of operation, selection of operation types and evaluation of operation dager.ConclusionThe spiral CT scan and 3D reconstruction can reveal fracture better.The advantages of SCT are suggested that this diagnostic modality may be the standard method for the initial evaluation of the upper cervical spine injury.

Objective To observe the effect of heat stress on plasma cGMP and hemodynamics in animals with chronic heart failure. Methods Coronary arteries of twenty-five rabbits were ligated to set up the animal model of heart failure and other five rabbits only received thoractomy as sham group. Eight weeks after coronary artery ligation, they are randomly divided into heat stress (HS) group, control group, HS+L-NAME group, and non-HS+L-NAME group and non-HS+L-Arg group, each group containing 5 animals. Hemodynamic indices and plasma levels of NOS and cGMP were measured. Results Hemodynamics of all 25 rats received operation was poorer compared with the rats of sham group (P

Objective: To study the effect of topical administration of basic fibroblast growth factor (bFGF) on healing of rabbit corneal wound caused by mustard gas. Methods: All the corneas of 8 New Zealand white rabbits were injured by liquid mustard at the concentration of 0.2%. One eye of each rabbit was treated with bFGF eye drops 6 times daily, the other eye with 0.25% chloromycetin eye drops as control. Computerized image analyzer was used to calculate the corneal epithelial healing rate. Results: The mean epithelial healing rate in bFGF-treated eyes was (1.276?0.152) mm 2/h, whereas in the control eyes it was (1.094?0.154) mm 2/h. The difference of 2 groups was statistically significant (P

Objective To clone CTLA-4 and TCRV?8 gene from T lymphocyte of thyroid of Graves' disease (GD) patients,recombine to form CTLA-4-TCRV?8 fusion gene and express the fusion protein.Methods CTLA-4 and TCRV?8 gene was cloned from T lymphocyte of thyroid of GD patients by RT-PCR.Then it was recombined with expression plasmid in order.The correct plasmids were obtained after the restriction analysis and DNA sequencing.Prokaryotic expression of the fusion protein in E.coli,SDS-PAGE and Western Blotting were used to verify the fusion protein.Results Restriction analysis and DNA sequencing confirmed the correct sequence and insertion site of the recombinant plasmid.The recombinant fusion protein was successfully expressed in E.coli,which was consistent with the predicted putative calculating molecular weight.Conclusion CTLA-4-TCRV?8 gene was constructed and expressed successfully,providing gene product and application theory for immune tolerance therapy of GD.

BACKGROUND: Active bone morphogenetic protein (BMP-2) reduction can cause severe symptoms like osteoporosis. In addition, there is estrogen receptor (ER) in osteoblast, osteoclast and bone marrow stromal cells, indicating a direct effect of estrogen on bone tissues.OBJECTIVE: To investigate the effect of Chinese medicine of Jiangzhi Zhuanggu on BMP-2 and ER expression in rats with prey.hyperlipidemia induced osteoporosis.METHODS: A total of 27 adult SD rats, of either gender, weighing 180-230 g, were randomly divided into three groups. In the normal control group, rats were intragastrically infused with 5 mL/kg normal saline every morning and afternoon. In the model group, the rats were infused with 5 mL/kg high-fat diet in the morning and 5 mL/kg normal saline in the afternoon. In the Chinese medicine group, 5 mL/kg high-fat diet was infused in the morning and 5 mL/kg Chinese medicine of Jiangzhi Zhuanggu water extract in the afternoon. After 8 weeks, expression levels of BMP-2 and ER in bone tissue was detected with immunohistological methods, and ER mRNA level of bone tissue in rats was detected by in situ hybridization.RESULTS AND CONCLUSION: Compared with the model group, the BMP-2 and ER expression in the bone tissue was significantly increased (P ＜ 0.01), and ER mRNA level increased following Chinese medicine treatment. Results show that Chinese medicine of Jiangzhi Zhuanggu could increase BMP-2 and ER expression in the osteoporosis bone tissue, and improve osteoporosis effectively.

Objecitve To investigate the relationship between placental growth factor and acute coronary syndrome(ACS). Methods 70 cases of patients with acute chest pain were enrolled. 36 cases of patients who is posi-tive on cardiac troponin T(cTnT) as treatment group, 24 patients who is negative on cTnT as control group, blood of all patients were detected about PLGF and cTnT in hospital,and coromary angiography examination, Gemini score is used to evaluate coronary artery lesion. Result PLGF and cTnT were elevated in ACS, it had significant difference between two groups(P <0. 01) ,PLGF and cTnT are significantly positive correlated(r= 1.2,P <0. 01), PLGF and cornorary artery lesion severity are significantly positive correlated(r = 1 ,P <0. 01). Conclusion PLGF is helpful to predict ACS attack to comorary heart disease,higher PLGF predicts unstable to coronary artery lesion in coronary heart disease.

Objectlve To study the therapeutic efficacy of the combined medication of ShiHuiDa and capto pril on patients with by Opertension and coronary heart disease.Methods 80 patients witll hypertension complicated with coronary disease were included in this study.They were randomly dirided in to treatment group and control group,every group is forty patients.Treatment group treated by ShiHuiDa and captopril,control group treated by hydrocblorothiazide nitrendipine and dinitrosorbide.Blood pressure angina pectoris relief and electrocardlogram were observed after eight weeks therapy.Results Systolic pressure and diastolic pressure in treatment group is less than control group,it is obviously better than control group about depressurization effect,it has significant difference by statistical test.Angina pectoris relief rate and electrocardiogram improve rate in treatment group is better than control group,it has significant difference.Conclusion The combined medication of ShiHuiDa and captopril is not only effective in lowering blood pressure and stronger and persistence,but also in relieving angna pectoris attack.

Objective To explore the diagnosis and treatment of isolated dissection of the superior mesenteric artery (SMA).Methods From Feb 2006 to July 2010,15 patients with isolated SMA dissection were treated in our center,there were 13 males,2 females,the mean age was(53 ± 8) years (range 43 -63).Among them,1 was caused by trauma,14 had unknown etiology,and 9 cases had a history of hypertension.Diagnosis was made by contrast-enhanced computed tomography (CT) in all cases.Management strategies inlcuded placement of self-expanding bare stent,medical treatment,and transperitoneal SMA fenestration.Results Endovascular stenting was attempted in 14 cases,with a success in 5 and a failure in 9 cases who were then given medical treatment with antiplatelet agents.One case with critical intestinal ischemia underwent open exploration and SMA fenestration.Blood vessel patency resumed.Follow-up with duplex and CT was accomplished in 13 cases,time ranging from 12 to 60 months (mean 28 ± 14mos).There was no recurrent abdominal pain or chronic intestinal ischemia developed during the follow-up.In medically treated patients,there was no aneurismal enlargement of SMA,while in the endovasculartreatmentgroup,allstentsremainedpatentthroughoutthefollow-up.Conclusions Endovascular treatment of isolated dissection of SMA appears to be feasible and effective,despite its relatively low technical success rate.For asymptomatic patients,medical treatment is the treatment of choice.In case of critical intestinal ischemia and with a suspected intestinal gangrene,emergency surgical exploration and fenestration should be performed.