60mg /kg?d ip, of paeonol were given to rats for 15 days. The myocardial ischemia reperfusion injury model was produced by occluding the left coronary artery and releasing the occlusion in rats. This significantly decreased myocardial Ca++-ATPase activitics and CH/PL radical, FFA content, improved mitochondrial membrane fluidity and kept them away from oxygen free radical damage. These results indicated that paeonol has membrane protective effect on myocardial ischemia rcperfusion injury probably by inhibiting the oxygen free radicals and subsequent lipid peroxidation.

Objective To investigate the emotional intelligence training training's impact on medical students' ethics education.Methods Givingnthe undergraduate and graduate students of Grade 2009,2010 and 2011 emotional intelligent training and distributing questionnaires on ethics education and comparing the result with that of 2006,2007,2008 undergraduate and graduates.Covering issues of medical ethics and the understanding of students' role in alleviating the doctor-patient relationship in medical ethics,evaluation of medical ethics in today's society,the doctor of professional ethics,medical ethics education approaches and problems and so on.By comparing the results of the questionnaire students' knowledge of medical ethics are evaluated and their emotional intelligence training in ethics education role are analyzed.Research data for entry to a database of Excel 2013,using SPSS 19.0 statistical software for analysis,count data taken x2 test,P＜0.05 was considered statistically significant.According to the questionnaire topic A representative proportion of medical students is selected to measure the level of ethics and the role of ethics education in medical students training in emotional intelligence.Results the students of 2006,2007 and 2008 who selected A of five topics were 54.78％(1 278/2 333),79.47％(1 854/2 333),68.41％(1 596/2 333),86.28％(2 013/ 2 333),13.12％(306/2 333); while the students of 2009,2010 and 2011 who selected A of five topics were 73.70％(1 777/2 411),88.68％(2 138/2 411),81.54％(1 966/2 411),93.78％(2 261/2 411),71.75％(1 730/2 411).The difference was statistically significant,indicating that 2009,2010 and 2011 medical students' cognitive level of ethicshas been nore significantly improved than the students of Grade 2006,2007 and 2008.Conclusion EQ training can significantly improve the effectiveness of medical ethics education for medical students and it is worth promoting.

0.999. Conclusion The precision and accuration of this method can satisfy the determination of VOCs in indoor air, this method is simple, rapid and easy to be popularized also.

Objective:To investigate the correlationship between levels of erythrocyte receptor type1(E-CR1) and disease activity and abnormal criteria of immunology in systemic lupus erythematosus (SLE)and to study the E-CR1 levels in pathogenesis of SLE.Methods:The fluorescence intensity in sera from 72 patients with SLE and 20 cases of normal controls was detected by flucytometry.Complement(C3,C4),immunoglobulins(IgG,IgA and IgM),gammaglobulins(?-G),erythrocyte sediment rate(ESR) and blood cell counts,including white blood cell(WBC),red blood cell(RBC),hemoglobin(HGB),hematocrit(HCT),mean corpuscular volume(MCV)and platelet(PLT) were detected at the same time.The scoring instruments of systemic lupus activity measure (SLAM) were used to evaluate disease activity.Results:The E-CR1 levels in patients with SLE were lower significantly than that in normal controls (P

Objective　To synthesize the artificial antigen methylparaoxon （M1600）.Methods　Methylparaoxon was reduced into amino-methylparaoxon by using acetic acid-zinc power-hydrochloric acid. Artificial antigens M1600-BSA， M1600-TTH　were synthesized by conjugating amino-methylparaoxon to bovine serum albumin （BSA）and tachypleus tridentatus hemocyanin （TTH）directly after diazotization.Results　 Rabbits had been immunized with M1600-BSA for 10 weeks， and the high titer and high specificity antiserum from those rabbits was testified by doubled agar gel diffusion and indirect ELISA．Conclusion　An artificial antigen was obtained successfully and this made it possible to establish the immunoassay of M1600.

Objective To discuss the efficacy of Lamiophlomisrotata Dripping Pill combined with intra-articular injection of medical ozone in the treatment of knee osteoarthritis.Methods Eighty patients with knee osteoarthritis were selected,the control group (39 cases) were administered Lamiophlomisrotata Dripping Pill,the observation group (41 cases) were administered Lamiophlomisrotata Dripping Pill combined with intra-articular injection of medical ozone.The efficacy of Lamiophlomisrotata Dripping Pill combined with intra-articular injection of medical ozone in the treatment of knee osteoarthritis were evaluated by efficacy,knee function,and inflammatory factor.Results After treatment,the effective rate of the observation group was higher than that of the control group (P ＜ 0.05).In the observation group,there were 38 cases of patients who felt pain,tenderness,and swelling symptom relief.There were 28 cases in the control group.After treatment,the scores of pain,activity,walking ability,and KSS in the observation group were higher than that of the control group (P ＜ 0.05).There was no statistical significance on the stability between two groups.Before treatment,there were no statistical significance on the IL-6,IL-8,and TNF-α.After treatment,the IL-6,IL-8,and TNF-αt were increased in two groups (P ＜ 0.05).And these indexes in the observation group were higher than that of the control group (P ＜ 0.05).Conclusion In summary,Lamiophlomisrotata Dripping Pill combined with medical ozone had good effect on the knee osteoarthritis.It could release the pain,improve knee function and anti-inflammatory.It was worthy of clinical use.

Objective To explore the cause,classification,incidence rate,diagnosis and treatment of pulmonary sequestration.MethodsThe data of 22 cases was analysed and the literatures were reviewed.ResultsThere were 22 cases of sequestration that confirmed by pathology after resected in our department from 1997 to 2007,of which 16 cases(72.7%)were confirmed before operation.The incidence rate was 5.7% comparing with the same time lobectomy.There were 20 cases(91%) of intralobar type and 2 cases(9%) of extralobar type.In intralobar type,18 cases were accompanied with infection and 2 cases with hemoptysis.2 cases had no symptom in extralobartype.ConclusionPulmonary sequestration is not an rare disease.The diagnosis mainly depends on X-ray.Sometimes,bronchography,retrograde arteriography or CT can also help making the diagnosis correctly.Operation is the main treatment method.Special attention should be paid to avoiding injure to the vessels during operation.

Objective To approach the cause,radiologic images feature and clinical diagnostic technique in pulmonary aspergillosis.Methods We retrospectively evaluated the clinical presentation,symptoms,x-ray film and radiologic images,and clinical diagnostic methods of 36 cases with pulmonary aspergillosis.Results All cases were cured though operation and conformed by pathologic diagnosis.28 cases were diagnose as pulmonary aspergillosis,4 cases as lung cancer,3 casses as pulmonary tuberculosis,1 case as pneumonia.Conclusion Pulmonary aspergillosis was usual secondary to pulmonary tuberculosis,radiologic images feature was contribute to diagnosis,pathologic proof and fungus isolation in infected pulmonary tissue were gold standard.

With the development of molecular and cell biology,new concept of the etiology of pituitary adenomas was taking shape.The etiology of pituitary adenoma was considered to be related to gene mutation and transforming factors.This paper reviews the roles of gene mutations,transforming factors,expression of receptors on cells and growth factors in the etiology of pituitary adenomas.

Objective To investigate the anti-oxidation effects of compound Chinese medicine of Yiqiling,on the spon- taneously hypertensive rats(SHR) and to explore the new target for improving the prognosis of hypertension.Method SHR were randomly divided into 2 groups:Yiqiling group and the control group.The serum content of malondiadehyde (MDA) and superoxide dismutase(SOD),and the urine content of 8-iso prostaglandin F2?(8-isoPGF2?) in two groups were measured.Examination of the gene expression profile was analyzed by using cDNA microarray.Three of the anti-oxidation genes were selected to undergo RT-PCR and quantitative real-time PCR.Results The 8-isoPGF2?urine level and MDA serum level were lower,and SOD serum level was higher in Yiqiling group than those in the control group,the differences of the above indexes had statistical significance.In Yiqiling group the expression of 354 genes was up-regulated and that of 121 genes was down-regulated in comparison with the control group.The results of the Hsphl,Hspca and Pam genes detected by RT-PCR and quantitative real-time PCR were consistent with those detected by the microarray examination.Conclusion Yiqiling has anti-oxidation effects and improvement on the prognosis of SHR,and its possible mechanism is related with the regulation of Hsphl,Hspca and Pam genes expression.