itamin D plays a crucial role in human health and diseases.Researches have showed that 50％ of the population have a risk for vitamin D deficiency in the world.Vitamin D deficiency is caused by the lack of sunlight or by deficient dietary supplement.It does not only lead to rickets and osteomalacia, can also cause cancers, cardiovascular disease, endocrine system diseases, autoimmune diseases, nervous system diseases and tuberculosis susceptibility.

Objective:To study the histogenesis and cell differentiation of desmoplastic ameloblastoma.Methods:The expression of various protein filaments was detected by immunohistochemical stainning in 20 cases of desmoplastic ameloblastoma and the ultrastructure of the tumor was observed in 4 cases.Results:CKH,CK8 and CK19 were positive in spindle-shaped and polygonal epithlium tumor cells.Vimentin was positive in mesenchymal fibrocytes and smooth muscle actin in some mesenchymal cells.Ultrastructural observation found that tonifilaments were in epithelial cells and there were desmosomes between these cells.In stroma myofibroblasts were presented.Loose connective tissue could be seen around some epithelial cell nests.Conclusion:Desmoplastic ameloblastoma may be arise from the early stage of the dental germ formation, epithelial cells of the tumor had the tendency to differentiate to the keratinized cells.

The paper introduces new retrieval functions in PubMed 2008, including advanced search pattern, citation sensor and improved automatic term mapping, meanwhile the analysis and assessment on those new functions are originally revealed, it points out the advantages, limitations as well as applicable situations, so as to provide help for users.

OBJECTIVE:To explore the reasons for leg length discrepancy following total hip arthroplasty,and to summarize the prevention and treatment of leg length discrepancy after total hip arthroplasty.DATA SOURCES:A computer-based online search was conducted in PubMed (www.ncbi.nlm.nih.gov/pubmed) for the English articles published between 2001 and 2008 with keywords "Leg length discrepancy,hip arthroplasty" and Wanfang database (http://www.wanfangdata.com.cn) for the Chinese articles published between 2004 and 2009.DATA SELECTION:Totally 154 literatures were retrieved by computer,including 116 English articles and 38 Chinese articles,through reading title and abstract,112 independent and 51 repeated papers were excluded.The remained 29 literatures were performed further analysis.MAIN OUTCOME MEASURES:Reasons for leg length discrepancy and the prevention and treatment of leg length discrepancy following total hip arthroplasty.RESULTS:The main cause of leg length discrepancy after total hip arthroplasty included redundant neck of femur stump,used longer prothesis neck,diminished femur eccentricity,selected longer neck of femur prothesis,as well as larger movement of cotyloid cavity rotation center.The effective approaches of leg length discrepancy after total hip arthroplasty included careful preoperative planning,intraoperative measurement digital templating and image-guided surgery.It could be the adjusted with heel and shaped bottom for the limbs length which was more than 15 mm after operation,and revision operation maybe applied to patients who had more than 20 mm limbs length or combined with complications.CONCLUSIONS:Leg length discrepancy is one of the common complications following total hip arthroplasty.Along with the development of science and technology,to design individual prothesis and computer-assisted navigation joint can be recover farthest joint function.

Objective To investigate the efficacy of repairing finger nail bed defects by split-thickness nail bed grafts in one stage, and evaluate the functional recovery of nail beds comprehensively. Methods From February 2000 to December 2003, 33 fingers with nail bed defect in 26 patients (7 thumbs, 13 index fingers, 9 middle fingers, 4 ring fingers) were repaired with middle thickness grafts of nail bed harvested from the residual nail beds of the same fingers or from nail beds of 1st, 2nd and 3rd toes. Brachial plexus block or regional anesthesia was used. When the area of nail bed defect area was less than one third of the nail, the graft was taken from the same finger under rubber tourniquet. In condition of the area of nail bed defect larger than one third of the nail beyond nail plica or two nail bed defects, the grafts were taken from the nail beds of 1st, 2nd and 3rd toes. Results In the study, there were 23 fingers (18 patients) with the nail bed defects area more than 5 mm and less than one third of the nail repaired with the grafts of the same finger nail; there were 10 fingers (8 patients) with the nail bed defects more than one third of the nail beyond the nail plica or two nail bed defects transplanted with toe nail bed. All patients were followed up from 6 months to 30 months with an average of 17 months, all grafts survived very well. No pain, no infection and obvious deformed growth of nail were found, and a sound recovery was obtained in all patients. Excellent and good rates were 87.9% in 23 patients with 29 fingers; poor rate was 12.1% in 3 patients with 4 fingers according to the criteria by L? Gui-xin. Conclusion The transplantation of middle split-thickness nail bed to repair nail bed defect is simple and easy to operate. The grafted nail can grow in good appearance, and the deformation rate is lower.

Objective: To sum up the therapeutic effect of early enteral nutrition(EN) in postoperative patients with esophageal or cardiac cancer.Methods: The clinical data of 106 postoperative patients with esophageal or cardiac cancer who had received early enteral nutrition treatment were analyzed retrospectively.Results: All the patients recovered well.The occurrence of the serious postoperative complication,such as anastomotic leak,hypoalbuminemia and so on had been decreased.Conclusion: Early EN for postoperative patients with esophageal or cardiac cancer is a physiological nutrition support way.EN can maintain gastrointestinal function and improve nutritional status.

Objective To study the imaging diagnostic methods of fibrous dysplasia.Methods 31 patients with fibrous dysplasia proved pathologically were studied. In addition to CT scan in 11 cases and MRI in 6 cases,plain films were performed in all 31 cases. The compartive analysis was made between the features of plain film and CT.Results Of 31cases, 18 patients had monostotic fibrous dysplasia and 13 polyostotic lesions. Roentgenologically, they were divisible into 4 type: cystic type(18 lesions),diffuse type(30 lesions),sponge groud-flesh type(2 lesions) and moth-eaten type(2 lesions). CT features of lesions were classified into 2 type: cystic expanded type(38 lesions) and osteosclerotic type(7 lesions).Conclusion X-ray film should be used as the first choice in diagnosis of fibrous dysplasia. CT is hightly sensitive in displaying the craniofacial lesions.The lesions can be correctly diagnosed in most instances,if plain film combined with CT.

Objective To investigate immunoadsorption in the prevention of hyperacute rejection in highly sensitized recipients of renal allografts.Methods Immunoadsorption treatment was performed on 10 patients whose panel reactive antigen (PRA) was more than 40 %. The adverse effect and hyperacute rejection were observed.Results Two cases of acute rejection of renal allografts were found in highly sensitized recipients and reversed by the therapy of immunoadsorption and adjusting immuno-suppressive drugs.Conclusion Immunoadsorption treatment could effectively and safely prevent highly sensitized recipients from hyperacute rejection post-transplantation.

Objective To research the quality control of determination of formaldehyde concentration in the indoor air in the newly decorated houses with 4-amino-3-hydrazine-mercapto-1, 2, 4-amilinoazaole spectrophotometry. Methods 35 newly decorated houses in two urban districts of Guiyang were chosen and the air samples were collected from March to December in 2004. Formaldehyde concentration was determined as soon as the air samples were collected, and the laboratory internal quality was controlled by using the standard sample in the determination. Results The formaldehyde concentration range of 35 newly decorated houses was 0.01-1.71 mg/m3, the detection rates was 100%, the rate of exceeding national standard was 77.1% and the median was 0.28 mg/m3. The recovery rates of quality control samples were 90.70%-103.00%. Conclusion The quality control method used in the present paper can make the results of formaldehyde concentration determinated with 4-amino-3-hydrazine-mercapto-1, 2, 4-amilinoazaole spectrophotometry be controlled in the demanded ranges of errors.

[Objective]To discuss a reasonable treatment for unstable intertrochanteric hip fractures in senile patients by comparing the effects of PFN fixation and hemiarthroplasty.[Method]Totally 82 intertrochanteric hip fractures in senile patients with complete clinical data were retrospectively analyzed,who were treated with hemiarthroplasty or PFN fixation from April 2005 to April 2007.Forty-six were treated with hemiarthroplasty,36 were treated with PFN fixation.All of them had multiple medical co-morbidities.According to modified Evens-Jensen classification,all of them belonged to unstable fracture.Comparison was made between the two treated groups in terms of the length of incision,operative time,the blood lost,blood transfusion during or after surgery,time for bed rest postoperatively,postoperative complications and St.Michael hip score one year after surgery.[Result]The average duration of follow-up for PFN fixation and hemiarthroplasty were 16 and 18 months respectively.Compared with the group of hemiarthroplasty,the group of PFN fixation experienced longer operation time,longer time for bed rest postoperatively,less blood lost,less blood transfusion during or after surgery,shorter incision length,and the differences between two groups had statistical significance (P0.05).[Conclusion]Both of the two methods are reasonable treatment for unstable intertrochanteric hip fractures in senile patients.