Objective　To study the induction of apoptosis and the regulation of apoptosis related genes bcl-2, bax and ICE expression by wild-type p53 gene. Methods　A p53 gene recombinant adenovirus vector, ad CMV p53, was transfected into human bladder cancer cell HTB9. The level of bax mRNA was quantified by reverse transcription-polymerase chain reaction (RT-PCR). The content of Bcl-2, Bax and ICE protein were detected by immunochemical staining. Agarose gel electrophoresis of DNA、 terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and flow cytometric technique were used for analysis of apoptosis. Results　Introduction of wild-type p53 could increase the mRNA and protein level of bax, and could induce apoptosis in HTB9 cells with the apoptotic percentage of 50.4%. At the same time, the introduction also increased the protein level of ICE, but that of Bcl-2 decreased.Conclusion　It's very possible that wild-type p53 gene induced apoptosis by regulating the expression of apoptosis-related genes including bax, bcl-2 and ICE.

Objective To investigate the safety and efficacy of Left atrial linear ablation of paroxysmal atrial fibrillation guided by three-dimensional electroanatomical system. Methods 29 patients with paroxysmal atrial fibrillation in this study. A nonfluoroscopic mapping system was used to generate a 3D electroanatomic LA mapping, and all pulmonary vein ostia were marked under the help of pulmonary veins angiography on the 3D map. Radiofrequency (RF) energy was delivered to create continuous linear lesions encircling the pulmonary veins. It was delivered with a target temperature of 43℃, a maximal power limit of 30W and applied for ≥20 seconds until the maximal local electrogram amplitude decreased by ≥50%. The ablation was completed by finishing the circular line. Results The mean procedure duration was 180?18 minutes, with mean fluoroscopy time of 80?20 minutes. The average number of RF pulses was 120?15. After a follow-up of 6.0 months, 24 patients maintained sinus rhythm. 3 patients suffered from less frequent paroxysmal atrial fibrillation during the first 3.0 months after the ablation and remained Af free after 6 months. 1 patient had atrial fibrillation episodes and 1 patient had atrial fibrillation attacks unchanged. No pulmonary vein narrowing was observed. Conclusion Left atrial linear ablation of paroxysmal atrial fibrillation guided by three-dimensional electroanatomical system was safe and effective.

Objective To investigate the clinical presentations,pathologic features,treatment and prognosis of lymphoepithelioma-like carcinoma(LELC)in the upper urinary tract. Methods A case with LELC of the ureter was retrospectively analyzed.A 81-year-old female patient was hospitalized because of gross hematuria for 1 month. CTU demonstrated a 2.5cm tumor in the left ureter.Radical nephroureterectomy was performed laparoscopically. Results Pathologic findings showed invasive poorly differentiated carcinoma in a prominent lymphocytic background.The diagnosis was LELC of the ureter.Epstein-Barr virus in situ hybridization was negative.No disease progression was noted at 3-month foUow-up. Conclusions LELC of the upper urinary tract is rare.All the cases are diagnosed pathologically.The disease eould be treated with radical surgery.Although the differentiation of the tumour cells is poor,the prognosis of pure subtypes seems to be better than that of other types of urothelial carcinoma.

Objective To investigate the efficacy and safety of 3D-laparoscopic surgery in adrenalpheochromocytomas /paragangliomas.Methods From December 2012 to July 2014,23 cases of adrenal pheochromocytomas/paragangliomas were treated by 3D-laparoscopic surgery.Among them,7 cases were male and 16 cases were female,whose mean age are 47 (32-68) years old.Their clinical symptoms including sustained hypertension in 11 cases,intermittent hypertension in 8 cases,sustained hypertension with intermittent progression in 2 cases and asymptom in 2 cases.All tumors were diagnosed by endocrine examination,image test and nuclear medicine.Mean diameter of tumors are 8 cm,ranged from 3-14cm.In order to adjust the blood pressure,patients took α-receptor blocker for 2-4 weeks preoperatively.Under the general anesthesia,19 patients received the 3D-laparoscopic surgery via retroperitoneal approach and 4 cases received the procedure via peritoneal approach.Results All operations were completed successfully.There were no major intraoperative complications.Mean operating time was (78 ± 21) min and mean estimated blood loss was (54.8 ± 36.3) ml.Mean hospital stay after operation was (3.8 ± 1.4) days.The pathological diagnosis included pheochromocytomas in 15 cases and paragangliomas in 8 cases.During the 3 to 18 months following up,no recurrence or metastasis was found in this study.Conclusions 3D-laparoscopic surgery has obvious advantage in spatial location and the sense of depth for adrenal pheochromocytomas /paragangliomas,which shortens the operation time and increase the safety obviously.

Radiomics is a new area of research, which converts imaging data into high?resolution quantitative imaging features by applying the automatic high?throughput imaging?feature?extraction algorithm. With the development of data science, more and more attention has been paid to the non?invasive and quantitative method in precision radiotherapy all over the world. This paper will briefly introduce the concept of radiomics and its application in precision radiotherapy.

Objective To observe the development of Schistosoma japonicum after single sexual infection and double sexual infection respectively. Methods A single Oncomelania hupensis snail was infected by a single schistosome miracidium. The lar?va were induced and released from the snail after 60?day incubation at 26℃. The mice were infected by the larva(single sexual infection)and dissected 40 days after the infection. All the worms were collected and the sex of the larvae was determined by the sex of the adult worms. Then,the mice and rabbits were infected by single sex of larvae(single sexual infection)and double sex of larvae(double sexual infection)respectively. The mice and the rabbits were dissected 40 days after the infection. All the worms were collected and measured under a microscope. Results All the male or female worms were collected from the mice and rabbits after single sexual infection. There were three main forms of worms after dissection of double sexual infection of mice and rabbits:folded mature male and female,male or female. Few folded male and immature female were found. Only the double sexual larva infected mice or rabbits had schistosome eggs in the liver and the liver had typical schistosome egg nodules. The sin?gle sexual larva infected mice or rabbits had no schistosome eggs or schistosome egg nodules in the liver tissues. The single male larva could develop to worms with the testis,and with a little smaller size compared to the mature folded male,while the single sexual infection female worm could not develop to the mature stage with much thinner and smaller compared to the mature folded female. Conclusions The male or female worms from single sexual infection are smaller than those from double sexual infection (mature worms?folded male and female). So it is necessary to check single sex worms in vessels of intestinal mucosa thoroughly in the sentinel mice when no schistosome eggs were found in the liver.

Objective To analyze the constitution of in-patient death causes in Sharxi Cancer Hospital from 2005 to 2010. Method Statistical analysis of 1277 hospitalized cases from 2005 to 2010 in Shanxi Cancer Hospital was retrospectively conducted. Results The overall case fatality rate of hospitalized patients from 2005 to 2010 was 0.86 ％ (1277/146820),the rates were 1.16 ％,1.05 ％,0.99 ％,0.85 ％,0.84 ％, 0.64 ％, respectively, with a declining trend, and the differences among them was statistically significance (x2 =45.763,P ＜0.001).Total mortality rate of male hospitalized patients (1.26％) was higher than that of women (0.53％),and the difference was statistically significant (x2=215.367,P＜ 0.001).Analysis of cancer death cause revealed that lung cancer possessed the leading cause of cancer death with the ratio of 36.4％(456/1253). The analysis of top 10 death causes showed that the majority of the population in cancer death causes were men,cadres of staff and workers,secondary school education level people,and people over the age of 60. Conclusion The consitution of in-patient deaths in Shanxi Cancer Hospital is defined, which could provide a scientific basis for disease prevention and control.

Objective To study the application of the combined detection of Golgi glycoprotein 73(GP-73),phosphatidylinositol proteoglycan 3(GPC3 )and percentage of AFP heteroplasmon(AFP-L3%)in the diagnosis of primary hepatocellular carcinoma (PHC).Methods The concentrations of GP-73,GPC3 and AFP-L3 were detected by enzyme-linked immunosorbent assay(ELISA) in 154 patients with PHC(PHC group),78 patients with cirrhosis(cirrhosis group)and 56 healthy subjects(control group).Then the detection results were statistically analyzed.Results The levels of GP-73,GPC3 and AFP-L3% in the PHC group were signifi-cantly higher than those in the liver cirrhosis group and the control group(P <0.05).The positive rates of GP-73,GPC3 and AFP-L3% in the PHC group were 66.2%,72.1% and 53.2% respectively.The positive rate in the combined detection of these three in-dices could reach 97.9%,which was higher than the sensitivity and accuracy in any single index detection and the combination de-tection.In the PHC group,the comparison between different levels of GP-73 and AFP-L3% with the AFP levels showed the statis-tically significant difference(P <0.05 ).Conclusion The combination detection of GP-73,GPC3 and AFP-L3% can improve the sensitivity and accuracy for diagnosing PHC and has reference significance in the differential diagnosis of early PHC.

Objective To investigate the relationship between microchimerism and immunologic tolerance in kidney transplantation recipients. Methods In samples of peripheral blood and urinary sediment from 176 females who received male donor's kidney,DNA and mRNA expression of specific fragment in Y chromosome were determined by PCR and RT PCR. Results The test results were positive for chimerism in 137 out of 176 recipients(77.84%),and negative in 39 cases (22.16%).The mean survival period in chimerism positive group was 8.9?3.7 years,and 5.2?3.9 years in chimerism negative group.The difference was statistically significant between the 2 groups ( P

ObjectiveTo discuss the safety and feasibility of transumbilical laparoendoscopic single-site resection of postperitoneal paraganglioma. MethodsFive patients with postperitoneal paraganglioma accepted transumbilical laparoendoscopic single-site surgery from October 2010 to October 2011.Four patients were male and 1 patient was female aged from 24 to 52 years with the average age of 38 years.Three patients presented with paroxysmal hypertension,1 patient with persistent hypertension,and the other with no symptom.The tumors were located on left postperitoneum in 3 cases (2 adjacent to left renal vessels,the other para-aortic),and right postperitoneum in 2 cases (1 adjacent to right renal vessels,the other between inferior vena cava and aorta).The diameter of tumors ranged from 2.5 cm to 6.0 cm.or-blocker was given for preoperational preparation.After 2 -4 weeks,transumbilical laparoendoscopic single-site resection of tumors were administered.Transparacolic route was selected in 4 cases and the other was operated through mesentery. ResultsAll the operations were successful.None was converted to conventional laparoscopic surgery or open surgery.The operative time ranged from 80 to 190 min,the mean was 150 min.The blood loss ranged from 50 to 150 ml,the average blood loss was 85 ml. No operation-related complications ocurred.Four cases with hypertension returned to normal blood pressure.All cases recovered uneventfully and discharged in 3 to 6 d after operation.Postoperational pathologic result proved the diagnosis of paraganglioma.The wound healed very well and the scars were occult.Follow-up was performed in all cases for 3 to 15 months.No recurrence was found.ConclusionTransumbilical laparoendoscopic single-site resection of postperitoneal paraganglioma could be safe and effective,and has good cosmetic effect.