Objective　To investigate the early changes and clinical significance of plasma endothelin(ET) and nitric oxide(NO) in patients with acute brain injury.　Methods　The plasma ET and NO concentration at 1 day after injury were checked with radioimmunoassay and Green method in 48 cases of acute brain injury, in 42 cases of non-brain injury and in 38 healthy volunteers. 　Results　 The early plasma concentrations of ET and NO were (109.73±12.61) ng/Lf and (92.82±18.21) μmol/L, respectively, in 48 cases of acute cerebral injury, which were higher than those in the non-brain injury group［(67.90±11.33) ng/L and (52.66±12.82) μmol/L, respectively, P<0.01］ and in the normal control group ［(50.65±17.12)ng/L and (36.12±12.16) μmol/L, respectively, P<0.001］. The amounts of ET and NO in the patients with severe brain injury were (116.18±18.12) ng/L and (108.19±13.28) μmol/L, respectively, which were significantly higher than that of the moderate brain injury group［(92.33±16.32) ng/L and (76.38±12.71) μmol/L, respectively, P<0.01］.The early levels of ET and NO were negatively related to the GCS scores of the patients with acute brain injury. The amounts of ET and NO were (126.23±15.23)ng/l and (118.18±10.12) μmol/L, respectively, in the patients with subdural hematomas, which were significantly higher than those in the patients with epidural hematomas ［(81.13±12.37) ng/L and(68.02±13.18) μmol/L, respectively, P<0.01］. The stable plasma concentrations of ET and NO at 14 days after injury in the patients with acute brain injury and with non-brain injury were similar to that in the normal controls. 　Conclusions　 ET and NO are related to the pathophysiological process in the early period of acute brain injury, and the values of ET and NO correlate positively with the clinical manifestations. The changes of plasma ET and NO can be regarded as important indexes to diagnose the severity of acute brain injury.

The marrow stromal osteoblasts from New Zealand rabbits were seeded on coral treated with L-polylysine, fibronectin and culture medium only, then the cells/coral composites were cultured in vitro. The cells/coral composites were observed for the process of cells growth and matrix formation at 7, 14 and 21 days after culture. With the aid of electron microscope,it was demonstrated that on the surface of the coral holes,which was treated with fibronectin, there were more adhering osteoblasts and matrix formation than those treated with both L-polylysine and culture medium after 7 and 14 days.Cell count in coral blocks was determined by doing a fluorimetric DNA assay. Although the samples treated with L-polylysine demonstrated higher cell count than the coral treated with culture medium after 7 days, there was no statistically difference between the two after 14, 21 days. At each time point, the samples treated with fibronectin showed higher cell count and alkaline phosphatase activity than the orals otherwise treated, and the cell count also increased with culture time. The study suggests that fibronectin has a significant effect on promoting the adhesion and proliferation of osteoblasts on the surface of coral.

The miR-17-92 cluster,consisting of six individual microRNAs,including miR-17,miR-20a,miR-18a,miR-19a,miR-19b and miR-92a-1,is a kind of typical oncogenic polycistron microRNA.MiR-19 (containing miR-19a and miR-19b),as the key oncogenic component in miR-17-92 cluster,is overexpressed in human cancers including lymphomas,leukaemia,lung cancer,breast cancer and multiple myeloma,and has been one of research focus in recent years.It is found that miR-19 promotes tumor growth,invasion and metastasis through negative regulation of target genes such as PTEN,PP2A,Bim,SOCS1,and is correlated strongly with PI3K-AKT-mTOR pathway.MiR-19 plays an important role in tumor genesis and development.

Objective To evaluate the causes of postoperative complications in severe liver rupture and explore the prevention experienees.Methods The clinical data of 66 liver rupture patients who underwent surgical treatment in our hospital from January 2005 to July 2012 were retrospectively analyzed.The commou complications after surgery and the treatment measures of the complications were sludied.Results There were 9 deaths of severe liver rupture after surgery and related complications were found in 22 cases,including bile leakage (5 cases),intrahepatic abscess (3 eases),subphrenic infection (3 cases),pleural effusion (8 cases),intra-abdominal hemorrhage (2 cases),stress ulcer bleeding (2 cases).Conclusions Using damage control theory could improve the cure rate.Precise liver transection and postoperative excellent management was able to reduce the incidence of postoperative complications in severe liver rupture.

Continuing medical education is one of major parts in medical education system of minimal invasive surgeon. Based on the characteristics of net continuing medical education such as freedom of learning, no regional restriction and abundant resource, combined with visualized teaching of minimal invasive surgery education, the novel training model of e-learning based on internet was applied to establish minimal invasive surgery continuing education system.

Objective To understand the epigenetic regulations of P16 during cellular replicative senescence and premature senescence induced by hydrogen peroxide of human embryonic lung fibroblasts(HEFs).Methods The normal HEFs were divided into young cells(22 PDL),mid-aged cells(35 PDL)and replicative senescent cells(49 PDL)during replicative senescence.The synchronously cultured 22 PDL HEFs were exposed for 2 h to 400?mol/L H_2O_2 at half confluence on daily basis.The procedure lasted for 4 consecutive days.And then the treated cells were cultured for another 7 days,called premature senescent cells.The mRNA level of P16 was detected by fluorescence quantitative PCR.The methylation level in the promoter region -846～-639 bp was observed by methylation-specific PCR(MSP).The histone modifications was detected by chromatin immunoprecipitation-QPCR assay,including acetylation for H3,H4 and methylation for H3(Lys4)and H4(Lys20).Results In the process of cellular senescence,the mRNA level of P16 decreased in mid-aged cells,but increased significantly in both replicative and premature senescent cells compared with that of young cells(P

Using Xanthine-Xanthine Oxidase system to generate superoxide ani-on, using H2O2 -Fe2 + system to generate hydroxyl radical, the role of scavenging oxygen free radical by Miltiorrhiza was studied by electron paramagnetic resonance and spin trapping. The result suggested that the scavenging rate of hydroxyl radical by Salvia Miltiorrhiza injection is 65%, and that of superoxide anion is 100%. This result suggests that scavenging cytotoxic oxygen free radicals may be one of important pharmacological mechanism of Salvia Miltiorrhiza.

Rabbit osteoblasts were isolated, expanded in vitro, and then seeded into coral-hydroxylapatite. The osteoblasts/coral-hydroxylapatite composite was were implanted into the muscles of rabbits′ back. Bone regeneration was observed 4, 8 weeks after implantation by histological and scanning electron microscopic examinations.The results showed that osteoblasts could adhere and proliferate well on the surface of coral-hydroxylapatite. 4 weeks after implantation osteoid tissue was observed, 8 weeks after implantation large amount of new bone was found. It suggested that osteoblasts/coral-hydroxylapatite composite may be used for bone tissue engineering.

The marrow stromal cells of adult New Zealand rabbits cultivated and induced in vitro were used to form MCCAB by mixing,seeding and solidifying methods with the aid of alginate. The MCCABs were auto-transplanted intramuscularly into the rabbits for 4 to 8 weeks. The alginate-cancellous bone matrix composites or the cancellous bone matrix alone were implanted as control. The effectiveness of bone formation was assessed by means of roentgenography and histology.The results showed that the osteogeneses of MCCABs were better than those of the alginate-cancellous bone matrix composites and of the cancellous bone matrix. In the MCCABs, both intramembranous and cartilaginous osteogenesis were seen with the former predominating. In the control, only slight cartilaginous osteogenesis was seen. The results suggested that the osteogenesis of the MCCABs constructed by using tissue engineering method was obvious when transplanted intramuscularly, therefore, this kind of tissue-engineered bone could be an effective way for clinical application.

To investigate the feasibility of using coral and other materials as scaffolds for bone tissue engineering, coral, coral hydroxyapatite(CHA), cancellous bone matrix and other natural biomaterials served as culture scaffolds of osteoblasts were manufactured. The results showed, in addition to PLA, PGA, PLGA and other synthetic polymers, some natural biomaterials are also ideal scaffolds materials for bone tissue engineering.