Objective:To study the significancy of P27 Kip1 expression, cell apoptosis and cell cycle distribution in the evaluation of sensitivity in oral cancer chemotherapy. Methods:40 cases of oral tumor were treated with MTX,CDDP and PYM by subcutaneous implantable drug pump.Operation was performed 2~4 weeks after the induction chemotherapy. Cell cycle distribution, apoptosis and P27 Kip1 protein expression in the removed tumor tissue were detected by fllowcytometry and indirect fluoressent assay pre-and post-treatment.Results:(1)After induction chemotherapy S,G_2M and PI ration in cell cycle distribution decreased,G_0/G_1, AP and FI_ p27 increased(P

OBJECTIVE:To summarize the materials, design, fit-shape and fixation techniques and clinical application of temporomandibular joint (TMJ) prosthesis.DATA SOURCES: An online search was undertaken to identify English articles about TMJ prosthesis published in Pubmed database from January 1998 to December 2006 using the key words of "temporomandibular joint, joint prosthesis".STUDY SELECTION: The collected literatures about TMJ prosthesis were sorted, and those with good pertinence were selected. For the literatures of the same field, those published recently or in authorized journals were selected.DATA EXTRACTION: Totally 69 literatures were collected, 28 were enrolled, and the other 41 were excluded, including 23 were irrelative with the aim of this study, and 18 were repetitive studies.DATA SYNTHESIS: Joint prosthesis is one of the fields that develop the fastest in orthopedic surgery in recent 30 years.TMJ replacement aims to enhance the function of TMJ, alleviate pain, and prevent serious complication. With the rapid developments of material science, tissue engineering, joint biomechanics and other related subjects, TMJ prosthesis has been significantly improved in the materials, design, fit-shape and fixation techniques. People have developed from simply imitating the outline form and mechanical motor of TMJ to pay more attention to its pathological function. It is very significant to trace the new progress in hip prosthesis and knee joint, and apply the good outcomes in the design and manufacture of TMJ prosthesis.CONCLUSION: Prosthetic material plays leading and promoting roles in the development of joint prosthesis, good design,fit-shape and fixation are the necessary conditions for prosthesis to act its role, and it is also necessary to investigate joint biomechanics. TMJ prosthesis should be further investigated, and it has good prospect.

objective: To study the effects of induction chemotherapy via subcutaneous implantable drug pump on local immune status. Methods: 47 cases with node negative oral cancer were treated with MTX, CDDP, PYM via subcutaneous implantable drug pump(in 27 cases), or via vein for case control trial(in 20 cases). Local immune status of tumor tissue was detected by immunohistochemical technique before and after treatment. Results: The effectiveness of treatment with the drugs via subcutaneous implantable drug pump (DSIP) was observed in 21 out of 27 cases (77.8%) and that with the drugs via vein(DV) was in 13 out of 20(65.0%) respectively( P 0.05), while the number of positive cells of CD 4 and CD 4/CD 8 in the cases treated with DV was decreased( P

Objective To investigate the effects of exercise on the activity of heme oxygenase-1 ( HO-1 ) and the expression of HO-1 mRNA and the mechanisms involved in the vascular smooth muscle tissue of rats with spontaneous hypertension (SHR). Methods Twelve male Wistar rats with normal blood pressure were used as control group ( group C ).Another 24 male rats with SHR were randomly assigned to one of 2 experimental groups ( 12 rats per group):an SHR group (group S) and an SHR treated group (group T).Rats in group T were treated with 60 minutes of unloaded swimming exercise 6 times a week for 9 weeks.Their blood pressure was measured once a week.After the nine weeks HO-1 activity as well as the expression of HO-1 mRNA in vascular smooth muscle and the concentration of carbon monoxide in plasma were measured. Results After the 9 weeks of training,average systolic blood pressure in group T was close to that of group C.The systolic blood pressure of group S continued to rise,and was significantly higher than that of the other 2 groups at each time point.Average HO-1 activity in group S (637.94 ± 73.637 ) reduced significantly compared with that of group C (786.20 ± 74.698) or with that of group T ( 1036.53 ± 140.63 ).That of group T was significantly higher than that of group C.The average expression of HO1 mRNA in group S (80.85 ± 6.953 ) was significantly higher than that of group C (45.15 ± 7.651 ) and lower than that of group T (90.70 ± 11.20),and the differences were statistically significant at the 5％ level of confidence.The average level of expression of HO-1 mRNA in the T group was significantly higher than that of group C.The plasma carbon monoxide content of S group was significantly lower than that of groups C and T. Conclusions Exercise can enhance the activity of HO-1 and the expression of HO-1 mRNA in vascular smooth muscle in rats with SHR to reduce blood pressure.

Objective To evaluate the expression and significance of P-glycoprotein(P-gp) in colorectal carcinoma. Methods The expression patterns of P-gp in 48 patients with colorectal carcinoma were examined by immunohistochemistry SP mothod and were analyzed its correlation to clinic opathological features. Results The expression of P-gp was related to lympy node metastasis. Its expression was not related to sex and age of the patients, localization of the primary tumor, tumorsize, histological grade, localinvasion, distant metastasis and Duke's stage. Conclusion Detecting the expression of P-gp might play an important role in chemotherapy strategy of colorectal carcinoma.

ObjectiveTo assess whether intravenous contrast medium would result in acute kidney injury (AKI), and to determine the risk factors associated with contrast induced AKI (CI-AKI) and its outcome.Methods A retrospective observational study was conducted in intensive care unit (ICU) of Fuyang People's Hospital in Zhejiang Province from January 1st 2011 to December 31st 2014. All enrolled critically ill patients had accepted CT scan, and the hospital length of stay was longer than 48 hours, and the patients who needed renal replacement treatment were excluded. Patients were divided into contrast medium group and control group. AKI was defined according to Acute Kidney Injury Network (AKIN) criteria (serum creatinine content over 26.4μmol/L or 50% increase of it from baseline within 48 hours). The incidence of AKI was compared between the two groups, and risk factors for CI-AKI were determined by multiple logistic regression analysis. The relationship of CI-AKI and outcomes were also analyzed. Results A total of 2 370 critically ill patients were enrolled during the period. 474 (20.0%) of the 2 370 patients received contrast medium, and 70 of them suffered from CI-AKI (14.8%). In 1 896 patients who did not receive contrast medium, 235 of them suffered from AKI (12.4%). There was no significant difference in the incidence of AKI between two groups (χ2= 1.905,P = 0.168). After several confounding factors were adjusted, multiple logistic regression analysis showed that contrast medium was not found to associate with AKI in critically ill patients [odds ratio (OR) = 1.66, 95% confidence interval (95%CI) = 0.72-3.90,P = 0.201], and high acute physiology and chronic health evaluationⅡ (APACHEⅡ) score (OR = 1.70, 95%CI = 1.33-2.40,P< 0.001), sepsis (OR= 8.06, 95%CI =3.28-17.80,P< 0.001), shock (OR= 3.57, 95%CI = 1.73-8.01,P< 0.001) and use of nephrotoxic agent (OR= 1.96, 95%CI = 1.25-2.63,P = 0.015) were risk factors of CI-AKI. Ten of 70 patients with CI-AKI died (14.3%), and 21 out of 404 patients without CI-AKI, died (5.2%). There was no significant difference in the mortality rate (χ2= 8.060, P = 0.005). It was shown by multiple logistic regression analysis that age (OR=1.30, 95%CI = 1.05-1.71,P = 0.027), male sex (OR = 1.13, 95%CI = 1.05-1.20,P = 0.039), APACHEⅡscore (OR = 1.07, 95%CI = 1.03-1.18,P< 0.001), and sepsis (OR = 3.29, 95%CI = 1.92-6.46,P< 0.001) were highly associated with mortality of critically ill patients in whom contrast medium was used. However, the occurrence of CI-AKI showed no influence on the mortality rate (OR = 1.70, 95%CI = 0.88-3.56,P = 0.227).Conclusions The use of contrast medium is not a risk factor of CI-AKI in critically ill patients. CI-AKI will not raise mortality rate in ICU patients.

Objective To assess the value of two-dimensional speckle tracking echocardiography (2D-STE) of predicting the acute response to cardiac resynchronization therapy (CRT) in patients with congestive heart failure. Methods Twenty-four patients with congestive heart failure scheduled for CRT were included. 2D-STE was performed within 7 days of implantation with device ON and OFF. Left ventricular (LV) dyssynchrony was defined as an interval ≥130 ms for the absolute difference in time to peak radial strain for the anteroseptal wall versus the posterior wall (T_(AS-POST)) with 2D-STE. Acute hemodynamic response was measured as LVdp/dt, and percentage change in LVdp/dt was used to classify responders (Δdp/dt%＞25%) and nonresponders (Δdp/dt%≤25%). Results Fifteen patients (62.50%) were classified as acute responders. Compared with nonresponders, the responders demonstrated significant increase of LV ejection fraction and reduction of TAS-POST after CRT-ON. T_(AS-POST)was the only determinants of Δdp/dt%＞25%. T_(AS-POST)≥130 ms prognosticated acute response to CRT with sensitivity of 86.24% and specificity of 70.38%. Conclusion CRT can immediately increase the LV systolic function and synchrony. 2D-STE is highly predictive for acute response to CRT.

Objective To study the effects of diagnostic ultrasound combined with microbubble contrast agent on augmentation thrombolysis in vivo and to discuss the synergism and mechanism of combination of urokinase. Methods Thirty-two rabbits of femoral vein thrombus were divided into four groups: simple urokinase group, ultrasound and contrast microbubble group, ultrasound and contrast microbubble combined with urokinase group, control group. Then according to the best parameter combination which was definited in previous experiment, the experiments combination of urokinase were performed. Color Doppler was used to evaluate the recanalization of different group in 15 rain,30 min and 60 min. Results After the treatment of 15 rain and 30 min,the recanalization rate in ultrasound and contrast microbubble combined with urokinase group improved significantly compared with other groups (P<0.05);after 60 min, the recanalization grade of ultrasound and contrast microbubble and urokinase group was evidently better than other two group. The result of immunohistochemistry showed that disaggregation of clot and fibrous in this group at 60 min was better than the results at 15 min, 30 min. Conclusions Diagnostic ultrasound with microbubble contrast agent showed superior effects of thrombolysis and the synergism of combination of urokinase in vivo.

Objective To investigate the relationship between the expression of the Smad4,TGF-?_1 and T?R II and the possible mechanisms in the development of cholangiocarcinoma. Methods Immunohistochemical SABC method was used to examine the expression of Smad4,TGF-?_1 and T?R II in 49 cases of cholangiocarcinoma and 8 nomal common bile duct.Results The expression rates of Smad4,TGF-?_1 and T?R II in cholangiocarcinoma were (40.82)%(20/49),71.43%(35/49) and 36.73%(18/49) respectively; in normal common bile duct tissues were 87.50(7/8),12.50%(1/8) and 100%(8/8) respectively. There was a significant relationship between the (expression) of TGF-?_1 and clinical stage, and tumor metastasis (P

Objective To observe the effects of trimetazidine (TMZ) on the cardiac function and neurohormonal of heart failure model in rats.Methods Partially banding abdominal aortic artery to achieve congestive heart failure rats model.Interventricular septum thickness(IVST),left ventricular posterior wall thickness(LVPWT),left ventricular end-diastolic diameter(LVEDD),left ventricular end-systolic diameter (LVESD),left ventricular ejection fraction(LVEF) and shortening fraction(FS) were measured by echocardiogram,Pathological changes of myocardial cells was observed,B-type natriuretic peptide (BNP)、C-type natriuretic peptide receptor (NPRC),atrial natriuretic peptide (ANP),myosin heavy chain (β-MHC) and angiotensinl (AT1) were measured by Real-Time PCR,superoxide dismutase (SOD) was measured by immunohistochemistry method.Results Trimetazidine treatment of the high-dose group and the model group compare IVST LVPWT,LVESD,LVEDD were (0.63 ± 0.05) mn,(0.73 ± 0.06) mm,(0.73 ±0.05)mm,(0.87 ±0.06)mm and (1.07 ±0.06)mm,(1.13 ±0.06) mm,(0.93 ±0.06)mm,(1.33 ±0.06) mm,was significantly reduced (P ＜ 0.05),LVEF,FS increased to (27.75 ± 1.83) ％,(11.44 ± 0.76) ％ and (11.78 ±0.56)％,(4.27 ± 0.22)％ (P ＜ 0.01),Myocardial cell structure were remarkably improved.The expression of BNP,ANP,NPRC,ATI,β-MHC were remarkably decreased.The expression of SOD was elevated.Conclusion TMZ treatment group can improve the secretion of neurohormonal of heart failure model in rats,and also obviously improve the cardiac contractility.