Objective To compare the therapeutic effects of tug-of-war needling method versus conventional electroacupuncture on knee osteoarthritis, seek a better method for treating this disease and prove the importance of needling and moxibustion techniques. Method One hundred patients meeting the criteria of knee osteoarthritis were allocated, in order of visits, to an electroacupuncture group of 50 cases and a tug-of-war needling group of 50 cases. In the tug-of-war needling group of patients, points Zusanli(ST36) and Heding(EX-LE2) were mainly selected and after the arrival of qi following needle insertion, forward pushing or backward pulling was repeatedly made like tug of war to strengthen or eliminate the needling sensation. In the electroacupuncture group, local points were mainly selected according to the routine, and after the arrival of qi, a G6805-1 type electric acupuncture therapeutic instrument was connected and sparse-dense waves were used. Reactions were recorded in the two groups of patients after two courses of treatment. Joint function was assessed using the VAS and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). Result Both groups of patients were followed up at six months after the end of treatment. There were statistically significant pre-/post-treatment differences in the VAS score and the WOMAC sub-scores and total score in the two groups (P＜0.01). The VAS score and the WOMAC sub-scores and total score decreased more in the tug-of-war needling group of patients than in the electroacupuncture group (P＜0.01). The total efficacy rates in the tug-of-war needling and electroacupuncture groups were 92.0% and 90.0%, respectively, at the end of treatment and 94.0% and 92.0%, respectively, at the follow-up;there was no statistically significant difference between the two groups and between at the end of treatment and at the follow-up in the two groups (P>0.05). However, the cure and marked efficacy rates in the two groups of patients were 68.0%and 52.0%, respectively, at the end of treatment and 68.0%and 54.0%, respectively, at the follow-up. It was statistically higher in the tug-of-war needling group than in the electroacupuncture group (P＜0.05). Conclusion Both tug-of-war needling and electroacupuncture have a good and very stable therapeutic effect on knee osteoarthritis. Tug-of-war needling is better than electroacupuncture in improving the clinical symptoms.

[Objective] To observe the effect of long-term gavage of Folium Sennae (FS) on colic electromyogram and interstitial cells of Cajal (ICC) in rats. [Methods] Forty rats were randomized into normal control group and FS group. The normal control group was fed with conventional forage. FS group was fed with dry forage mixed with FS powder for 3 months, the preliminary dose of FS was 200mg?kg-1?d-1 and then increased progressively by 100mg?kg-1?d-1 to ensure over half of the rats having diarrhea, and the final dose was 2800 mg?kg-1?d-1. The frequency and amplitude of colic slow wave were detected and the changes of myenteric plexus and ICC were observed by zinc iodide -osmic acid staining method. [Results] After long-term gavage of FS, the frequency and amplitude of colic slow wave in rats obviously decreased ( P

Familial hypercholesterolemia(FH) is a common autosomal dominant dyslipidemia,which is caused by mutations of the low-density lipoprotein receptor(LDLR) gene producing defect or deficiency in LDLR.The characters of FH are elevated level of total and LDL cholesterol.FH is considered to be a complex polygenic disease.Recently,more findings indicate that proprotein convertase subtilisin/kexin type 9(PCSK9) gene play an important role in serum cholesterol metabolism.Some mutated PCSK9 proteins decrease LDLR,which cause FH in affected families.Some other mutated PCSK9 proteins decrease self-affinity,which cause hypocholesterolemia.We review the newest researches about the structure,function ofPCSK9 gene and the relation of its mutations with plasma cholesterol metabolism.

Objective:To study the feasibility of digital subtraction angiography(DSA) and selective external carotid embolization(SECE) in the diagnosis and treatment of maxillofacial tumor. Methods: DSA features, embolotherapy, clinical treatment and complications were investigated retrospectively in 22 cases diagnozed by means of DSA and treated with SECE. Results: After embolization, the bleeding was completely stopped in two cases with emergent hemorrhage, the tumor was shrunk in one case with central hemangioma in maxilla three weeks later and the bleeding in operation was reduced significantly. The tumor boundary was clear for tumorectomy in all the other cases. No recurrence was observed in 3-year-follow up. Conclusion:DSA and SECE are safe, optimal and effective in the treatment of maxillofacial tumour and hemorrhage.

Objective:To observe the clinical efficacy of nerve-near needling for occipital neuralgia.Methods:Eighty-two subjects were randomly allocated into two groups:treatment group in which 46 cases was treated by nerve-near needling[points Tianzhu(BL 10)and Tianyou(TE 16)were selected],and control group in which 36 cases were treated by conventional acupuncture[points Tianzhu(BL 10),Fengchi(GB 20),Baihui(GV 20),Shimian Ⅱ (Extra),Wangu(GB 12)and Hegu(LI 4)were selected].One course of treatment consisted of 3.5 times,and three courses of treatment were given at most before calculating efficacy.Results:The short-term total effective rate was 93.5%in the treatment group and 69.4%in the control group,with a statistically significant difference between the two groups(P＜0.01);the long-term total effective rate Was 64.1%in the treatment group and 43.3%in the control group,with a statistically significant difierence between the two groups(P＜0.05).Conclusion:Nerve-near needling is better than conventional acupuncture in treating occipital neuralgia.

SOCS3 has significant regulation effects in cell signal transduction pathways, which can be induced by many kinds of cytokines and proinflammatory factors. After being studied for years, the effect of SOCS3 has become clear in maintaining physiological functions and affecting histopathologic changes in human tissues. This review presents the role of SOCS3 in the occurrence, development, diagnosis and treatment of human diseases, such as inflammation, virus infection, obesity and tumor. As abnormal levels or impaired function of SOCS3 were reported in the onset and development of disease, SOCS3 can be considered as a bio-marker to diagnose and predict prognosis of some disorders, and as a therapeutic target for certain diseases.

The process of tumor angiogenesis is a complicated chain reaction which happens under the action of a series of positive feedback and negative feedback factors . Cancer stem cells are small part cell groups of self-renewal and multilineage differentiation potential in tumors. Many studies indicate that tumor stem cells participate in the process of tumor angiogenesis, and vascular endothelial growth factor can regulate endothelial proliferation and angiogenesis, thereby promoting tumor growth and metastasis.

Inflammatory microenvironment plays a key role in the tumor recurrence and metastasis.The inflammation of tumor microenvironment can start the tumor metastasis by regulating epithelial-mesenchymal transition ;can improve the tumor invasion by degradation the extracellular matrix;in addition to,inflammation can supply appropriate microenvironment for tumor vascular angiogenesis.

Atherosclerosis (AS) is pathologically important basis of many kinds of coronary atherosclerosis disease (CAD). It can be substantially protected by raising high-density lipoprotein (HDL).In view of mechanism, drugs for raising HDL include: cholesterol ester transfer protein inhibitors, peroxisomal proliferator-activated receptor agonists, liver X-activated receptor agonists, farnesoid X receptor antagonists or agonists, lipoprotein lipase activators, niacin, and phenytoin and lecin : cholesterol acyltransferase activators, etc. This review aimed to the progress of drugs for regulating high-density lipoprotein and their mechanism, in view of clinical and preclinical aspects.

OBJECTIVE:To study the chemical constituents of Glycyrrhiza pallidiflora.METHOD:IR, 1 H-NMR,13C-NMR,MS and TLC were used to identify conpounds .RESULTS:Five compounds were elucidated as homopterocarpin,β-sitosterol,4＇,7-dimethoxyisoflavone,medicarpin and isoglabrol. CONCLUSION:The 4＇,7-dimethoxyisoflavone was first separated from this plant .