To find out the health need of the city population in Guangzhou and the service level of the city's medical units and to set up a public health service system to answer the need of the market e- conomy,an investigative analysis is here focused on the health state and health service need of the city's population,the allocation of the health resources and the utilization of the city's health resources,eco- nomic factors,ways of medical payment,the cultural and age composition of the population structure, and the impact of the service personnel quality on the utilization of the service.It is suggested that the government increase its fund appropriation on public health,establish neighborhood medical leagues, start medical insurance,speed up cooperative medicine in the countryside,and complete the medical se- curity system.

Objective To reduce the complications of male indwelling urinary catheter and improve patient compliance by improved the traditional male urinary catheter.Methods Inspired by using ‘condom’‘fresh-keeping bags’and other pseudo catheter in clinic,the tradi-tional male urinary catheter was improved as‘pseudo catheter ’and the hydrocolloid dressing was used as bonding device.Results An im-proved male urinary catheter was successfully designed,which applied in 68 patients without urinary tract infection,catheterization failure nor foreskin edema.Conclusion The improved male urinary catheter has the advantages of less cost,simple operation,no pain,higher patient com-pliance and less complications,wihch is worthy to spread in clinic,especially in young patients and long-term bedridden elderly patients at home.

Objective To understand the microbiological pollution of drinking water dispensers and find out the effective disinfection method for dispensers. Methods The counts of bacteria, fungi and yeast in water samples collected from the water stored in liners of 52 drinking water dispensers were determined. The total counts of bacteria in water samples of 8 water dispensers were determined before and after disinfection by 1/10 YOUJIE disinfectant for 2 min. Results The medians and over standard rates were 440 cfu/ml and 96.15% for the total count of bacteria, 0 and 30.77% for fungi and yeast in the water samples before disinfection respectively. After 2_min disinfection by 1/10 YOUJIE disinfectant, 5 of 8 drinking water dispensers showed bactericidal rates of 100% and other 3 water dispensers showed bactericidal rates of 99.56%, 99.77% and 99.78% respectively. Bacillus was found in some unkilled bacteria. Conclusion Microbiological pollution in water stored in liners of drinking water dispensers was high, which could be effectively eliminated and disinfected by 1/10 YOUJIE disinfectant for 2 min.

AIM: The purpose of this study was to investigate the effects of rhBMP-2/collagen composite on bone regeneration during expansion of the interparietal suture in the rats. METHODS: 32 10-week old SD rats were divided into groups consisting of 8 rats each. They were comprised of normal control group, expansion control group and the treatment group, the two treatment groups were covered with atelo-typeⅠcollagen and rhBMP-2/collagen composite on the suture before subjected to the expansion force. The bone regeneration in the interparietal suture was estimated by histological method, the osteocalcin content was measured by radioimmonoassay and the calcium content was determined by atomic absorption spectrophotometer. RESULTS: The bone regeneration was more active in the suture after giving an expanding force than in the suture without any intervention. Even bone bridge was formed in the rhBMP-2/collagen composite group. Both the osteocalcin content and calcium content were much higher in the rhBMP-2/collagen composite group than in other three groups (P

Objective To investigate the clinical value of the thickness of pharyngeal wall of nasopharynx in adenoids hypertrophy examined with CT. Methods CT manifestations in 117 patients suspected with adenoids hypertrophy were analysed. The thickness of adenoid and pharyngeal wall were observed. The width of adenoid/pharynx(A/N) ratio were measured and the correlation of pharyn-geal wails with A/N ratio were analysed. Results A/N ratio was ≥0.70 in 50 cases (group Ⅰ), between 0.60 ～ 0.70 in 35 cases (group Ⅱ) ,≤0.60 in 32 cases (group Ⅲ) ,the thickness of pharyngeal wall were (1.26±0.33) cm, (0.99±0.41) cm and (0.86± 0.19) cm respectively. There were significant differences between group Ⅰ and Ⅱ, Ⅲ in the thickness of pharyngeal wails(P<0.01). The relationship of linear regression of A/N ratio with pharyngeal wall was found and the equation of the thickness of pharyngeal wall was 1.69×A/N ratio-0.08. Conclusion There is correlation between A/N ratio and thickness of pharyngeal wall, which is helpful for clinical diagnosis of adenoid hypertrophy.

Objective To develop a rapid and sensitive DNA microarray for Listeria monocytogenes detection.Methods A DNA microarray was developed using gyrB,ISR,16S rRNA,23S rRNA,hlyA,iap and prfA as the target genes and tested against 18 different species of known reference for repeatability,sensitivity,and specificity to verify the effectiveness of the chip.Results After testing of samples by the LM array,results show that the 70 mer Oligos synthesized by IDT are superior to the Oligos synthesized by Sagon with respect to both probe spotting or samples detection.The comparison of 3 spotting probe concentrations of 10 μmol/L,40 μmol/L and 80 μmol/L demonstrated that the 10 pmol/L probes result in good detection signals equivalent to the 40 μmol/L and 80 μmol/L probes.The repeatability and sensitivity evaluated by sample testing on the LM array revealed that the chips developed in this study have good repeatability and the lower limit of sample detection is 0.9 ng DNA.The LM array can distinguish clearly and definitively between Listeria and non-Listeria bacteria in the sample.Conclusion The microarray is able to rapidly detect and identify Listeria monocytogenes.

In recent 20 years, the most important advancement of rectal cancer surgery is the total mesorectal excision(TME) and laparoscopic minimal invasive surgery. However, visualization of the distal rectum is still troublesome because of narrow pelvic, bulky tumor, and especially obese male patients. A possible solution for this situation is distal rectal mobilization by transanal minimally invasive surgery (TAMIS), or even pure transanal total mesorectal excision (taTME). When people applaud a new era of rectal cancer surgery is coming, we must also concern about the patients selection, the long-term follow up results and even the operative safety.
Abdomen ; Anal Canal ; Digestive System Surgical Procedures ; Humans ; Laparoscopy ; Male ; Minimally Invasive Surgical Procedures ; Patient Selection ; Rectal Neoplasms

In recent 20 years, the most important advancement of rectal cancer surgery is the total mesorectal excision (TME) and laparoscopic minimal invasive surgery. However, visualization of the distal rectum is still troublesome because of narrow pelvic, bulky tumor, and especially obese male patients. A possible solution for this situation is distal rectal mobilization by transanal minimally invasive surgery (TAMIS), or even pure transanal total mesorectal excision (taTME). When people applaud a new era of rectal cancer surgery is coming, we must also concern about the patients selection , the long-term follow up results and even the operative safety.

In recent 20 years, the most important advancement of rectal cancer surgery is the total mesorectal excision (TME) and laparoscopic minimal invasive surgery. However, visualization of the distal rectum is still troublesome because of narrow pelvic, bulky tumor, and especially obese male patients. A possible solution for this situation is distal rectal mobilization by transanal minimally invasive surgery (TAMIS), or even pure transanal total mesorectal excision (taTME). When people applaud a new era of rectal cancer surgery is coming, we must also concern about the patients selection , the long-term follow up results and even the operative safety.

Objective:To retrospectively analyze the long-term survival (10-15 years) and late toxicity of nasopharyngeal carcinoma (NPC) patients after intensity-modulated radiotherapy (IMRT), aiming to provide reference for the optimal treatment of NPC.Methods:132 patients with NPC who were treated with IMRT in Sichuan Cancer Hospital from 2003 to 2009 were recruited. Among them, 3 patients were classified as stage Ⅰ, 22 cases of grade Ⅱ, 61 cases of grade Ⅲ, 43 cases of Ⅳ A and 3 cases of Ⅳ B, respectively. The median dose was 73.37Gy (66 to 85Gy), divided into 33 times. Twenty patients received radiotherapy alone, 112 cases of concurrent radiochemotherapy. The survival rate was calculated by Kaplan-Meier method and log- rank test. Univariate prognostic analysis was performed. Cox model was used to conduct multivariate prognostic analysis. The late radiation toxicity was evaluated by RTOG/EORTC criteria. Results:The median follow-up duration was 128 months (range, 3 to 191 months). The 10-and 15-year local control rates of NPC patients were 86.0% and 79.9%. The disease-free survival rates were 72.5% and 63.2%, and the overall survival (OS) rates were 65.2% and 57.1%. The local recurrence rate was 12.1%, and the distant metastasis rate was 16.7%. A total of 53 patients died, of whom 15 patients died of local recurrence, 20 patients died of distant metastasis and 18 patients died of other diseases (pneumonia, intracranial hemorrhage and accident, etc.). The 10-and 15-year non-tumor-related mortality rates were 11.3% and 13.6%. Univariate analysis showed that age, smoking habit, lactate dehydrogenase (LDH), T stage and clinical stage were the independent prognostic factors of OS in NPC patients. Multivariate analysis demonstrated that LDH, T stage and synchronous chemotherapy were the prognostic factors of OS in NPC patients. The incidence of gradeⅠ-Ⅱ late radiation injury (hearing impairment, dysphagia, dental caries and xerostomia) was 90.4%, and 8.5% for grade Ⅲ-Ⅳ late radiation injury (skin fibrosis, hearing impairment and radiation brain injury).Conclusions:The 10-and 15-year OS of NPC patients treated with IMRT is relatively high. With the prolongation of survival, the non-tumor-related mortality rate is increased. Distant metastasis is the main cause of treatment failure. The main late injuries include grade Ⅰ/Ⅱ hearing impairment, dysphagia, dental caries and xerostomia.