Objective To investigate the HOMA-IR and HOMA-? index in the diagnosis and judge the patients with vesting traumatic hemorrhagic MODS in the clinical and prognostic value,as well as the role of insulin resistance and decreased insulin secretion in energy metabolism disorder of patients with MODS.Early prevention and treatment of clinical provided for MODS.Methods 60 patients with traumatic MODS and 30 healthy adults participated in the study,were measured and compared their fasting blood glucose,insulin,lactate and pyruvicacad,glucagon and cortisol levels,calculated HOMA-IR,HOMA-?,and the lung,liver,kidney,pancreatic ? cells,and other major organ function in MODS patients parameters and indicators of the level of cells,HOMA-IR index were relevant and regression analysis.Results MODS group HOMA-IR index,fasting plasma glucagon and cortisol were significantly higher than the normal control group(P

Objective To study the effects of mild hypothermia on secondary cerebral vasospasm and levels of endothelin (ET),calcitonin gene related peptide (CGRP) in cerebrospinal fluid (CSF)and plasma in patients with severe brain injury(SBI).Methods Sixty-five patients with SBI were randomly divided into mild hypothermia group(30 cases)and control group(35 cases).Based on the routine treating method,the patients of mild hypothermia group were treated with local mild hypothennia.The levels of ET,CGRP in CSF and plasma were detected before and after treatment.And the incidence rates of secondary cerebral vasospasm cases were compared between the two groups.Results (1)At 7,14 day after treatment,ET levels in CSF and plasma in mild hypothermia group were significantly lower than these in control group (P＜0.05or＜0.01).The CGRP levels in CSF and plasma were the lowest at 7 day,then gradually increased in two groups.However,the CGRP levels in mild hypothermia group at 7,14 day were higher than those in control group (P＜0.05 or＜0.01).(2) The rate of secondary cerebral vasospasm in mild hypothermia group was 6.67% which significantly less than that in control group (22.86%) (P＜0.05).Conclusion Mild hypothermia can prevent the increasing of ET and the decreasing of CGRP in CSF and plasma,and decrease the incidence rate of secondary cerebral vasespasm in patients with SBI.

[ABSTRACT]AIM:ToobservetheeffectofthesignalingpathwayofproteinkinaseC(PKC)-nuclearfactor-ery-throid 2-related factor 2 (Nrf2) on the expression of heme oxygenase-1 (HO-1) induced by cigarette smoke extract (CSE) in the rat airway epithelial cells .METHODS:After exposed to CSE , the airway epithelial cells of male SD rats were di-vided into control group , CSE3 group, RO318220 group, Nrf2 siRNA group and RO318220+Nrf2 siRNA group.The pro-tein levels of HO-1, Nrf2 and PKC were semi-quantified by Western blotting .The protein expression of HO-1 was assessed by immunocytochemistry .The mRNA expression of HO-1 was detected by reverse transcription-polymerase chain reaction ( RT-PCR) .Nrf2 protein location was observed by immunofluorescence staining .HO-1 activity was also determined .RE-SULTS:After exposure to CSE for 3 h, the Nrf2 protein was mainly located in the nucleus , and the expression level of Nrf2 protein in the nucleus was stronger than that in the control cells .CSE also significantly enhanced the levels of p-PKC protein, and HO-1 mRNA, protein and activity .Pretreatment with RO318220 significantly decreased the levels of PKC pro-tein, Nrf2 protein, and HO-1 mRNA, protein and activity.Meanwhile, Nrf2 protein significantly decreased , and the activ-ity, mRNA and protein levels of HO-1 were also significantly attenuated by pretreatment with siRNA to knock down Nrf2. Pretreatment with RO318220 and Nrf2 siRNA significantly decreased the PKC protein , Nrf2 protein, and HO-1 mRNA, protein and activity .CONCLUSION:CSE up-regulates the HO-1 expression through PKC-induced nuclear translocation of Nrf2 in the rat airway epithelial cells .

Objective To explore the role of IEGs in the central neurotoxicity of mice induced by anatoxin-a ANTX-A. Methods 80 Kunming mice were divided into groups and treated with ANTX-A at 200, 50 and 12.5 ?g/kg respectively i.p. for one month, 0.9% sodium chloride solution was used as the control. The expression of c-fos and NGFI-A genes and CREB protein in mouse brain were determined by using RT-PCR and immunocytochemical methods. Results The expression of c-fos gene significantly increased in the females of 50, 200 ?g/kg groups and in the males of 200 ?g/kg group compared with the control group. The expression of C-FOS and CREB-1 protein was significantly higher in 200 ?g/kg group than that in the control group. In all ANTX-A treated groups, the expression of NGFI-A gene did not show a significant difference compared with the control. Conclusion c-fos and CREB-1 genes may play a role in the central neurotoxicity induced by anatoxin-a.

Objective:To investigate the biological characteristics of dental epithelial cells derived from cervical-loop epithelium in rat lower incisor. Methods:The apical end of the lower incisor was dissected from two-day-old Sprague-Dawley rats by visual microscopy, and dental epithelial cells were obtained by culture of the apical end explants of the lower incisor. The biological characteristics of dental epithelial cells were determined by morphology and immunocytochemistry fluorescence. Results:Dental epithelial cells emigrated from the cervical loop of rat lower incisor, and epithelium-like morphologies with round-shape or square-shape was observed. Dental epithelial cells had a striking proliferation potential with a high level of BurdU labeling. As a marker of epithelium stem cells, P63 expression was observed in most early cultured cells. However, as cells were subcultured, dental epithelial cells,with the lower level of BurdU labeling,decreasing expression of P63 and increasing expression of alkaline phosphatase, were inclined to terminal differentiation.Conclusion:Stem cells or dental epithelial progenitor cells might reside in the cervical loop of the apical end of the rat lower incisor. The present study may provide an excellent cell source for study on the differentiation of dental epithelial cells and tooth regeneration.

Objective: To investigate the correlation between neck lymph node metastasis and focal adhesion kinases(FAK) expression in oral squamous cell carcinoma(OSCC). Methods: Immunohistochemistry LsAB technique was used to observe the expression of FAK in primary tumour and nodal metastasis of OSCC in 80 cases, correlation between the expression of FAK in OSCC and neck lymph node metastasis was analyzed statistically. Results: The results demonstrated that FAK was expressed in all 80 cases, the expression of FAK in the peripheral area of OSCC was stronger than that in central area (P

Objective To study the expressions of XIAP and survivin in breast carcinoma tissues and their(value) in predicting the recurrence of breast carcinoma.Methods The expression of XIAP and survivin in 65 benign and 65 malignant breast paraffin-embedded specimens was detected by using immunohistochemical assay and the results were compared.Results The positive rate of XIAP and survivin in recurrent breast carcinoma(recurrent group) was 82.2% and 85.7% respectively,and in non-recurrent breast carcinoma tissue(non-recurrent group) was 54.5% and 45.5% respectively,and in both groups were increased significantly as compared with that of benign breast tissues(20.0%,and 13.3% respectively).The positive expression of XIAP and survivin was increased significantly in recurrent group as compared with non-recurrent group(P

0.05).Measurement results of adjacent segment movement extention showed more significant changes in the fusion group than in the non-fusion group(P

Extracellular matrix remodeling plays a role in the pathogenesis of variety of diseases.Among these extracellular proteinases,Cathepsin is a key member.In this review,the relationships between Cathepsins and many diseases associated with extracellular matrix remodeling is discussed.

Objective To evaluate the clinical efficacy of sodium tanshinone IIA sulfonate(STS)in the treatment of ad-vanced schistosomiasis. Methods Fifty cases with advanced schistosomiasis admitted to the Touzao Township Hospital of Dong-tai City during the period from November 2012 to November 2013 were treated with STS for 10 days,and the internal diameter of the portal vein,levels of ALT,AST,γ-GT,PIIIP,CIV,HA and LN were measured and compared before and after the adminis-tration of STS. Results The mean levels of all serological parameters except HA were within the normal range before STS treat-ment,while the highest positive rate was detected inγ-GT(26.0%)and HA(54.0%). Following the STS treatment,the mean lev-els of all parameters and the positive rates reduced,with the greatest reduction observed inγ-GT(36.7%)and HA(37.8%);how-ever,the mean HA level was still higher than the normal range. The mean internal diameter of the portal vein reduced from(10.5± 1.7)mm before the STS treatment to(9.8±1.3)mm after the STS administration,with a significant diffrtence(P<0.05). Conclu-sion STS appears effective in the treatment of advanced schistosomiasis.