Objective:To determine whether p53 protein overexpression correlated with early tumor recurrence in carcinoma of maxillary sinus.Method:Forty-five patients with T2NoMo squamous cell carcinoma of maxillary sinus were treated with operation and/or radiotherapy . The median follow up time was 3 years. The ratio of local control was 62.2% (28 patients) , as control group ; The ratio of local recurrence and/or metastasis was 37.8% (17 patients),as experiment group . Cancer specimens were obtained before treatment.p53 protein overexpression, which indicated a mutated p53 gene ,was analyzed by immunohistochemistry, using the mouse monoclonal antibody DO-7.Result:Approximately 88.2% of the carcinoma that recurred locally overexpressed p53 protein , compared with only 28.6% of those with local control (P<0.01) .No significant relation was noted between p53 protein overexpression and pathohistologic grade .Intensity of staining did not predict tumor recurrece.Conclusion:The study demonstrated that the p53 protein is an important prognostic marker on gene level for maxillary sinus carcinoma.

Objective To establish a model of rat endotoxic pulpitis induced by Lipopolysaccharide (LPS);to study the dynamic expression and location of Oct-4B in rat pulpitis model at different time;To evaluate the role of Oct-4B on pulp injury and reparation in rat endotoxic pulpitis. Method The slides were made and the dynamic expression and location of Oct-4B was examined by immunohistochemistry. Results Oct-4B was moderately positive in the pulp fibroblasts and odontoblasts in 2 weeks. After 2 weeks, it was still positive in odontoblasts and fibroblasts. Up to 3 weeks, it was weakly positive in odontoblasts and fibroblasts. Conclusion Oct-4B is dynamically expressed in the rats with pulpitis, and may play an important role in the incidence,development and recovery of pulpitis.

This paper deals with the development of Paragonimus westermani (Kerbert, 1878) in mice inoculated with metacercariae or stunted adolescariae. In metacercaria-inoculated mice, all worms were stunted all the time, but when the stunted worms were orally transferred Into mice, some (8.4%) grew so fast that their rudimentory reproductive organs appeared and their length was over 3 times that of stunted worms 3 weeks post inoculation; while 91.6% of the recovered adolescariae were still stunted and morphologically similar to excysted metacercaiae. When the stunted worms were repeatedly transferred through mice by oral route 8.2-10.1% of the recovered worms developed quickly, while the remainder was still stunted (Table 1).After 3 transfers in mice, the stunted worms were still able to infect dogs orally and reach maturity (Table 2).On the basis of the study, it is postulated that cannibalism and preying among wild rats would enable P. westermani to be transferred among rodent paratenic hosts.

BACKGROUND: The grafted human hair keratin(HHK) artificial tendon,being degradable and absorbable, can induce formation of neotendon. In the present study, the main biomechanical indexes of HHK artificial tendon and its components were measured so as to determine a proper ratio for the artificial tendon composition according to different needs of the body sites and make it possess tensile stress required for functional activities.OBJECTIVE: To determine the main biomechanical indexes of HHK artificial tendon and its components.DESIGN: An observational study based on HHK artificial tendon.SETTING: Department of Biochemistry and Molecular Biology, Medical Biomechanical Key Laboratory of Chinese PLA, and Department of Histology and Embryology, a military medical university; Foshan Kangxing Medical Technology Co., Ltd.MATERIALS: The experiment was completed at the Medical Biomechanical Key Laboratory of the First Military Medical University of Chinese PLA in October 2001. The material of HHK artificial tendons was uncontaminated black hair collected from healthy young women in remote mountainous areas free from endemic diseases. There were three components of HHK artificial tendons with different in vivo absorption velocities Z, B and F. The in vivo absorption was the slowest for Z, faster for B, and the fastest for F. Different kinds of HHK artificial tendon were the mixture of Z, B and F at a proper ratio. Each group had 3 kinds, namely, Z3.0-20, B3.0-20, and F3.0-20. HHK artificial tendon consisted of 6 kinds: ZBF1. 0-20,ZBF1.5-20, ZBF3.0-20, ZBF6. 0-20, ZBF9. 0-20, and ZBF12.0-20.METHODS: Both ends of an HHK artificial tendon or each of its components were fixed on MTS 858 Moni Bionix checker to determine such biomechanical indexes as fracturing pull and tensile stress.MAIN OUTCOME MEASURES: Fracturing pull and tensile stress of HHK artificial tendon of different kinds and its components.RESULTS: Fracturing pull in Z3.0-20, B3.0-20 and F3.0-20 groups was (211.8 ± 12. 1 ), ( 178.8 ± 8.2), and ( 151.3 ± 6. 7) N, respectively; tensile stress was(71.3 ±3.9), (59.9 ±2. 7), and(50. 3 ±2.3) Mpa, respectively. Fracturing pull and tensile stress were decreased gradually in the same cross-section area of the 3 kinds of materials; Fracturing pull in ZBF1.0-20,ZBF1.5-20, ZBF3.0-20, ZBF6.0-20, ZBF9.0-20, and ZBF12.0-20 groups was(75.0 ± 3.0), (107.8±4.2), (194.1±5.3), (375.9±12.7),(508.2 ±21.3), and(670.8 ±25.4) N, respectively, while tensile stress was(75.0 ±3.0), (72.0 ±2.8), (65.1 ± 1.8), (62.9 ±2.2), (56. 3 ±2.4),and(55.8 ± 1.5) Mpa, respectively.CONCLUSION: Fracturing pull of HHK artificial tendon is increased with the increase of cross-section area, whereas tensile stress is slightly decreased with the increase of cross section area. With the increased extent of human hair treatment, its absorption in vivo is accelerated, and fracturing pull and tensile stress of HHK artificial tendon are decreased.

Due to the theoretical and technique limitation of traditional surgery,surgical treatment of complex intrahepatic biliary diseases was left for an unresolved difficult problem of the last century.Uncertainties of the anatomical,physiological,pathological changes and surgical techniques in the intrahepatic biliary tract contribute to this complexity.Through integrated application of modern scientific technology and traditional medicine methods and systematic optimization and innovation of biliary surgical theories and techniques,authors have developed a paradigm of precision biliary surgery which is characterized by high quantification,visualization and controllability.The establishment of the precise biliary surgical system solves the difficulty in surgeries for intrahepatic biliary diseases,puts forward the biliary surgery from extrahepatic ducts,hilar ducts to intrahepatic ducts,entering a new segment era of the biliary surgery marked by precision treatment of intrahepatic biliary diseases.

Objective To evaluate the possible protective effect of isoflurane and propofol on myocardium during off pump coronary artery bypass grafting (CABG) surgery. Methods Twenty-six patients undergoing elective CABG without cardiopulmonary bypass were allocated into propofol group (n=13) and isoflurane group (n=13). Anesthesia was performed in propofol group with propofol plus fentanyl, while isoflurane and fentanyl were used in isoflurane group. Artery blood samples were collected before induction of anesthesia, and during and after the surgery. Plasma troponin I (cTnI), creatine kinase-MB (CK-MB), interleukin-6 (IL-6), and lactate concentrations were determined respectively. Results At the 4 hours after operation, plasma level of cTnI was significantly increased in both groups, and no significant differences were found between them. Compared with baseline values, IL-6 in propofol group showed no significant changes at each endpoint. However, IL-6 values in isoflurane group were significantly higher than those in propofol group during and after surgery (P

Objective To study the expressions of XIAP and survivin in breast carcinoma tissues and their(value) in predicting the recurrence of breast carcinoma.Methods The expression of XIAP and survivin in 65 benign and 65 malignant breast paraffin-embedded specimens was detected by using immunohistochemical assay and the results were compared.Results The positive rate of XIAP and survivin in recurrent breast carcinoma(recurrent group) was 82.2% and 85.7% respectively,and in non-recurrent breast carcinoma tissue(non-recurrent group) was 54.5% and 45.5% respectively,and in both groups were increased significantly as compared with that of benign breast tissues(20.0%,and 13.3% respectively).The positive expression of XIAP and survivin was increased significantly in recurrent group as compared with non-recurrent group(P

Objective:To evaluate the long-term effect of augmentation rhinoplasty with silicone. Method:360 patients underwent augmentation rhinoplasty with silicone. Improved L shaped implants were used in 343patients. Result: Among 360 patients, 334 cases were successful enough (92.83 ％0 ). Conclusion: The key to themaintenance of the long-term effect is to use properly improvedL-shaped implant,to choose a suitable tunnel,to prevent various complications and to ask patient's opinions for implant design.

OBJECTIVE To summarize and evaluate the therapeutic studies on obstructive sleep apnea-hypopnea syndrome(OSAHS)in our country. METHODS Articles of therapeutic studies on OSAHS published in major journals of our country from Jan. 1995 to Dec. 2004 were evaluated retrospectively. RESULTS A total of 201 articles were recruited. The number of articles published every year was increased with time and most of the articles were retrospective study. There were no standard criteria for evaluating the curative effect,and the cure rate of UPPP were obviously different according to different evaluation criteria. In same evaluation criteria,the short- term outcome were better than long-term outcome,and the patients underwent multiple levels surgery had better outcome than single UPPP. CONCLUSION Multi-center,large sampled and prospective random comparison therapeutic study based on evaluative criteria constituted by Chinese Medical Association should be carried out in the future study. The impersonal methods and criteria for evaluation the upper airway anatomic structure and pharyngeal wall resilience should be established. Individuality therapy should be done. And finally, bring on therapeutic effect of OSAHS to a higher level in our country.

8 hours and without and binding nursing in these 3 groups respectively. Observed the condition of respiratory tract infection in 3 groups. Results The incidence rate of respiratory tract infection in binding nursing groups was 50%, which was significant higher than that of in no binding nursing group (P8 hours group was 66.67%, and this rate had an tendency of rising with the time of binding nursing lasting (P