Objective To investigate the dynamic CT features of peripheral lung cancer (PLC) and pulmonary inflammatory pseudotumor(IPT).Methods 23 patients with PLC and 6 patients with IPT were undergone plain and dynamic enhanced CT scans (beginning at 30 s after the onset of injection,serial scan were obtained at 30 s intervals within 180 s) before surgery or administration of antibiotic.Results The average CT values of plain CT,CT enhanced attenuation at 30 s and 90 s were significantly different between PLC and IPT.There were no significant decline following peak enhancement in IPT but PLC within 180 s.Conclusion The comprehensive analysis of plain CT value,enhanced CT value at 30 s,and the decreasing rate after 90 s of administration of contrast material may be useful in differential diagnosis between PLC and IPT.

Objective To study the relationship between expression of p16 and tumor angiogenesis as well as value of CT enhancement in peripheral lung cancer. Methods twenty-three patients with peripheral lung cancer were examed with dynamic CT scan before surgery.The MVD、protein expression of VEGF and p16 were measured immunohistochemically on speciments of resected tumors.Results There were negative correlation tendency between expressions of p16 and VEGF,but the difference were no significant.During the course of delaying 30 s from injecting contrast dose,the relating coefficient was the highest between enhanced CT value and MVD,P=0.9364.The mean value of MVD and CT enhancement in the course of delaying 30 s in p16 negative expression group were higher than that in the group of p16 positive expression with no significant.Conclusion p16 negative expression may improve angiogenesis in peripheral lung cancer.

AIM:To understand the interaction between a195- kilodalton protein,P195, on the surface of Plasmodium falciparum merozoite and human erythrocyte.METHODS:P195 was expressed in eight fragments in E.coli.After being refolded,the expressed proteins were la- belled with12 5 I,and incubated with human erythrocytes.RESULTS:According to binding assay, three fragments of P195:M3,M6,M9were found to have ability to bind to human erythrocyte. M6,which is equal to amino acid( AA) sequence from384 to595,could bind to human erythro- cytes but not to trypsin treated human erythrocytes,and the binding could be eluted by low p H buffer solution. M3( AA 12 3to 30 2 ) and M9( AA 10 78to 12 51) also have the ability to bind to human erythrocytes,but the binding was not affected by trypsin treatment and low p H buffer elu- tion. CONCL USION:The binding site of M6might be a surface protein receptor of human ery- throcytes,while the binding site of M3and M9might be an intracellular componentof human ery- throcyte.

Objective To observe the effect of intensive insulin therapy (ⅡT) on prognosis of severe chest trauma (SCT) pateints.Methods 42 consecutive patients were randomly assigned to 2 groups:ⅡT group (n =21) and the conventional insulin therapy group (CIT group,n =21).Blood glucose was maintained at the level of 4.4 -6.1mmol/L in ⅡT group,and 10.0 -11.1mmol/L in CIT group.The 2 groups were observed in terms of fatality rate during hospital stay,infection rate,the duration of mechanical ventilation,ICU stay length,pleural drainage day and count of neutrophils.Results No death or hypoglycemic reaction happened during hospitalization.Compared to CIT group,infenction rate,mechanical ventilation time,ICU stay length,and the time required for white blood cell to resume normal in ⅡT group were decreased significantly ( P ＜ 0.05 ).The difference had no statistical significance between the 2 groups in duration of pleural drainage and incidence of hypoglycemia (P ＞ 0.05).Conclusion ⅡT improves the short-term prognosis of SCT patients.

Objective To investigate the MRI imaging features, and pathologic basis of the orthotropic transplantation nude mouse model with human pancreatic cancer. Methods Adopting Siemens Magnetom Trio Tim 3.0 Tesla superconductive MRI and breast coil was used to examine 30 orthotropic transplantation nude mouse models of the human pancreatic cancer, these mouse were sampled to acquire TSE-T1 -weighted and T2-weighted transverse axial images. Intraperitoneal injection of Gd DTP A was used to perform continuous dynamic enhancement scanning. Signal intensities of tumors were measured in plain scanning and each phase' s enhancement scanning images, respectively. Intensification rates of tumors were calculated. Pathologic examination of tumors was performed to be compared with the findings of MRI scanning. Results The successful rate of inoculation of 30 nude mice was 100%. The histological findings were comparable with poorly differentiated adenocarcinoma. Compared with signal of adjacent tissues, the MRI findings of the tumors were uniformly slightly hypointensity (90% , 27/30) , or unevenly (10% , 3/30) on TSE-T1WI; uniformly (20% , 6/30) or unevenly (80% , 24/30) hyperintensity with equal or more hyper signal spots on TSE-T2WI. Signal intensities on plain scanning was 228.35 ±11.71, and 1.5,3,6,9, 12 min after enhancement scanning, thesignal intensities were 258.20 ± 11.17, 301.75 ± 17.09, 358.65 ±25.13, 480.05 ± 19.01, 558.35 ± 40.49, which were significantly higher than those in plain scanning (P <0.01). The intensification rate of every phase was 0.13 ±0.04, 0.35 ±0.11, 0.56 ±0.10, 1.10 ±0.10, 1.45 ±0.18, and the difference among these phases was statistically significant (P <0.01). The significantly intensified area was the area where the tumor cells grew actively with rich capillaries; the central area without intensification was the area of necrotic tissue and/or densely packed tumor cells and few capillaries. Conclusions High resolution MRI imaging of implanted tumors can be obtained by intraperitoneal injection of contrast, and it is consistent with pathologic examinations.

Objective To establish a stable orthotopic transplantation nude mouse model of the human pancreatic cancer and to explore the role of monitoring tumor growth with noninvesive MRI.Methods The tumors cells suspension made by the subcutaneous injection of human pancreatic cancer cell lines PANC1 were used as the source of tissue for orthotopic implantation of tissue.and transplanted into the pancreas of 20 BALB/C-nu nude mice.After implantation,the successful rate,tumor formation time,tumor growth speed,tumor shape and the change of signal of the tumor were monitored and recorded noninvasively by MRI.At the end of the 7th week,all the specimens were examined by pathological methods.Results Thirty-five percent (7/20)mice with implantation of primary human PANC1 adenocarcinoma cells were detected to have orthotopic implanted tumors by MRI after 15 days,and all the 20 nude mice developed pancreatic tumor within 27 days after operations,and the successful rate was 100%.Compared with adjacent normal tissue,the T1 WI imaging of 90%(18/20)of all the tumors showed uniformly hypo-intense signal,10%(2/20)showed iso-intense signal,the T2W 75%(15/20)showed uniformly hyper-intense signal.The tumor size 2,3,4,5,6,7 weeks after implantation was(912.6±2.4)mm3,(94.3±11.2)mm3,(175.9±82.5)mm3,(395.8±126.6)mm3,(1290.2±167.2)mm3,(1583.4±87.4)mm3,respectively.Pathologic examination confirmed poody differentiated pancreatic adenocatcinoma and it remained the primary tumor's biolosic features.Conclusions The orthotopic transplantation nude mouse model was in accordance with the feature of human pancreatic cancer and was easy for noninvasive monitoring,which provided an effective and stable in vivo experimental system.

The basic medicine teaching must meet the clinical application,while the clinical practice should be timely feedback to basic medical teaching,which is the embodiment of the idea in the translational medicine.The experimental teaching is an important part of modern pathology teaching.We actively improve the experiment course and deepen the basic concept of translational medicine in the medical education development by adopting such approaches as using the problem-based learning method(PBL) to promote the link between basic courses and clinical pathology,integrating and optimizing the teaching content to promote the knowledge mastery and conducting molecular pathology research to promote the implementation of translational medicine.

Objective Sperparamagnetic material is widely used in tumor localization and treatment , but there are still few studies in bone tissue engineering .This study aims to investigate the osteogenic efficiency of superparamagnetic scaffolds poly lactic acid hydroxy acetic acid (PLGA)/iron-doped hydroxyapatite (Fe-HA) for repairing rabbit mandibular defects , as well as evaluating the biocompatibility of the material . Methods 36 New Zealand rabbits were randomly selected and randomly divided into 6 groups, control without and with static magnetic field ( SMF ) groups ( no material is implanted), PLGA scaffold without and with SMF groups (implanted PLGA), PLGA/Fe-HA scaffold without and with SMF groups ( im-planted PLGA/Fe-HA), each group has 6 rabbits.We created bilat-eral mandibular defect models , executed the rabbits at 4 weeks and 12 weeks post-surgery, marked the mandibular specimens which detected by general observation, Micro-CT and HE staining, and ana-lyzed the results of bone mineral density in defect area .The hematological detection , pathological examination of liver and kidney sam-ples were carried out at 12 weeks pot-surgery.. Results The PLGA/Fe-HA scaffold without and with SMF groups began to appear rough defect area edge , decreased defect diameter , new bone formation from 4 weeks; the defect area formed a smooth , continuous bone repair from 12 weeks.The PLGA scaffold without and with SMF groups appeared rough , irregular callus repair , visible boundary between defect edge and normal bone tissue .There are still have part of unrepaired bone defect in the center of defect area in the control without and with SMF groups.At 12 weeks , the bone mineral density of PLGA/Fe-HA scaffold with SMF group [(572.50±19.09) mgHA/cm3] , respectively, compared with the PLGA scaffold with SMF group [(467.00±6.24)mgHA/cm3], the control with SMF scaffold group [(480.00±2.08) mgHA/cm3], the PLGA/Fe-HA scaffold without SMF group [(461.00±19.79)mgHA/cm3], the PL-GA scaffold without SMF group [(446.00±11.31)mgHA/cm3] and the control without SMF group [(422.00±28.28) mgHA/cm3], all the differences were statistically significant ( P<0.05).The bone mineral density of PLGA/Fe-HA scaffold with SMF group at 4 weeks [(572.50±19.09)mgHA/cm3] was significantly higher than that at 12 weeks [(276.00±28.28)mgHA/cm3] (P<0.05), the differences between 4 weeks and 12 weeks in other groups were statistically significant (P<0.05). Conclusion The superparama-gentic PLGA/Fe-HA scaffolds can promote the repair of bone defect , optimize the osteogenic effect of the material with SMF .Also ,the scaffold showed a good biocompatibility .

Objective To explore the dynamic change and correlation of the behavior and hippocampal volumes by MRI of rat depressive model. Methods Based on the evaluation of ethology, thirty SD rats were randomly and equally divided into control group and model group which was exposed to chronic unpredictable mild stress for 8 weeks. The body weight, fluid consumption test and Morris water maze test were done at the beginning and per week while the bilateral hippocampal volumes of every rat were measured by high field MRI at the beginning and per two weeks. Results Based on the behavioral data,the rat depressive model was established successfully,and changed dynamically. On 4th week,the left hippocampal volumes of depressive rat were significantly reduced (model group(37.13 ±2.40)mm3 ,control group (39.05 ±2.05)mm3, t=2.36, P＜0.05) while the right ones did not. On 6th week, the right ones began to reduce significantly ( model group ( 37.85 ± 2.11 ) mm3, control group ( 39.44 ± 2.10 ) mm3, t = 2.07, P ＜ 0.05 ) while the left one still reduced, but the extent of the left's reduction was bigger than the right( left:15% ,right:8% ). And there was apparent correlation between the behavior and the reduction of hippocampal volumes. Conclusion The behavior and hippocampal volumes of rat depressive model changes dynamically,and there was a regularity of them.

OBJECTIVETo study the clinical effect of root amputation in the treatment of periodontal/alveolar abscess teeth with one severe lesion root.

METHODS30 periodontal/alveolar abscess teeth with one severe lesion root were chosen in the study. After root canal treatment, supragingival scaling, subgingival scaling and root planning, occlusal adjustment were done. Then the teeth were treated by root amputation. The clinical effect was evaluated 3 months, 6 months and 1 year after surgery.

RESULTSOne year after surgery, 27 of 30 teeth were successful, 1 mandibular molar occurred root fracture, 1 mandibular molar was removed because of tooth loosening secondary to periodontal damage. 1 patient lost.

CONCLUSIONRoot amputation is an effective solution of periodontal/alveolar abscess.