Objective To detect the expression of SDF-1 and CD34,and MVD changes at slen der narrow pedicle flap of hypoxia and ischemia during the survival process,and to investigate the role of SDF-1 at flap neovascularization.Methods The slender narrow pedicle flaps,which pedicle's length-breadth ratio were 4 ∶ 2,and the flap size of each took an area of 2 cm × 2 cm (group A),3 cm ×3 cm (group B),4 cm × 4 cm (group C),5 cm × 5 cm (group D) and 6 cm × 6 cm (group E),were designed and elevated on each pigs' double dorsum.The flaps were served as a hypoxia/ischemia flap model.The survival area,histologic analysis,SDF-1 and CD34 enzyme-linked immunosorbent assay at the distal flap were evaluated at days 0,3,5,7,and 14 after operation,respectively.Results In the each group of flaps,the expression of SDF-1,CD34 and MVD increased with time,reached the plateau level after 5 days (maximal values of SDF-1:124.80 ± 4.05 ingroup A,137.85 ±3.03 in group B,166.53 ± 2.98 in group C,72.80 ± 2.63 in group D and 62.79 ± 2.20 in group E),7 days ( maximal values of CD34:16.76 ± 0.62,17.60 ± 0.72,18.48 ± 0.55,12.70 ± 0.60,and 11.51 ± 0.70,each group),and 7 days (maximal values of MVD:52.45 ± 2.78,59.34 ± 3.12,61.14 ± 3.35,25.25 ± 3.78,and 24.46 ± 7.46,each group),and then gradually decreased.In the different groups of flaps,when the flap area increased,the expression of SDF-1,CD34 and MVD increased,but the parameters decreased at the area of 5 cm × 5 cm,and the flaps were partial necrosis.Conclusions SDF-1 may play an adjusted role in the survival process of the slender narrow pedicle flap.

Objective To reveal the relationship between NOS,CD34 expression and the flap survival area,and to explore the survival mechanism of narrow strip flap,by designing different skin flaps with a slender narrow pedicle in a certain proportion of length to width on pigs' backs.Methods Five narrow flaps were formed on each side of the five white domestic pigs' back,of which pedicle width length ratio were 2 cm:4 cm,the area of the flaps was 2 cm × 2 cm,3 cm × 3 cm,4 cm ×4 cm,5 cm × 5 cm,and 6 cm × 6 cm,respectively,and named as flaps A,B,C,D,and E in turn.Besides,the flap A was taken as control.After the operation,the flaps were evaluated with general obveration,and NOS and CD34 expression and survival area were analyzed.Results For groups A,B and C flaps,the expression level of NOS and CD34 gradually elevated as the flap increased in size,and flaps completely survived (P＜0.05) ; For flaps in groups D and E,when distal end of flap was partial necrosis,the expression level of NOS and CD34 no longer elevated with the increase of skin flap area,and their expression was relatively constant (P＞0.05).Conclusions The survival area of the skin flaps is not in proportion to the width of its pedicle.Thus there is a maximum survival area of a skin flap carried by slender narrow pedicle in a certain proportion of length to width.

Objective To investigate the application and survival mechanism of a long and narrow pedicle flap which was used to repair the tissue defects after removal of tumor in aged patients.Methods The long and narrow pedicle flap was designed with its pedicle located beside wound surface along the pathway of well-known or perforating branch blood vessel to repair the defect caused by removing the tumor in aged patients.The size of the flaps ranged from 3 cm × 4 cm to 10 cm × 12 cm.The length and width of the pedicle ranged 2 - 8 cm and 1.0 - 1.5 cm.Results This flap was used in 17 cases.The flap was survived well in 14 patients,and healed later in other 3 patients because of wrong bandaging in earlier period.Conclusions The capability of bearing ischemia and hypoxia of the flap tissue is stronger after transfer owing to low metabolic rate in the skin tissue in aged patients.The flap is easily survived by repairing the tissue defects after removal of tumors in aged patients with the long and narrow pedicle flap.The pedicle of flap is narrow and long,and the transposition of the narrow pedicle flap is easy.The postoperative appearance is satisfactory.It is an ideal choice for repairing the defect caused by removal of tumor in aged patients.

Ultrasonic plane wave imaging is one of the hot topics in the study of ultrasound imaging, but the plane wave ultra-high frequency ultrasound has brought a large amount of data. If these data are transmitted directly through the hardware, it will not only bring a great burden on the hardware, but also put a requirement for the ability of the hardware processing data. In order to reduce the problem caused by the high frame frequency and large amount of data, the data compression methods are used to reduce the amount of data acquisition and transmission. In this paper both lossy compression and lossless compression are studied, where LZW compression algorithm is chosen to test lossless compression effect, and based on the plane wave characteristics, the frequency domain cutting compression algorithm is developed as a lossy compression method. In this paper, the LZW algorithm is implemented in FGPA, and it can achieve a certain compression ratio after testing the ultra sonic simulation data. Under the condition of known fractional bandwidth, it is found that the main bandwidth can be preserved in the spectrum, while the rest of the frequency signal has little effect on the image. By testing the ultrasonic simulation data, the frequency domain cutting algorithm can achieve high compression ratio, and the difference between the original image and the reconstructed image is relatively smal . So from the point of the compression ratio, the frequency domain cutting algorithm is the primary choice of the ultrasonic data compression algorithm.

Abstract OBJECTIVE To determine the index weight, optimize the extraction process of gastropathy oral thick paste 1. METHODS Taking the amount of water added, extraction time and extraction times as factors, depend on the single factor experiment, L9(34) orthogonal table was selected to design the experiment. The content of chlorogenic acid was determined by HPLC and the content of total flavonoids content was determined by ultraviolet spectrophotometry. With the above two contents plus the yield of dry extractum as the evaluation index, the weights were assigned based on the analytic hierarchy process, entropy weight method and independence weight method, and the extraction process was optimized by orthogonal experimental design. RESULTS Multi-index scoring was more scientific and reasonable, and the final optimal extraction conditions for gastropathy oral thick paste 1 were as follows: add 8 times the amount of water, extract twice and 0.5 h for each time. CONCLUSION The optimized extraction process using analytic hierarchy process, entropy weight method and independence weight method is reasonable, feasible, stable, and reproducible, which is suitable for the preparation of gastropathy oral thick paste 1, providing reference for subsequent research and development.

Zika virus (ZIKV) is an emerging pathogen associated with neurological complications, such as Guillain-Barré syndrome in adults and microcephaly in fetuses and newborns. This mosquito-borne flavivirus causes important social and sanitary problems owing to its rapid dissemination. However, the development of antivirals against ZIKV is lagging. Although various strategies have been used to study anti-ZIKV agents, approved drugs or vaccines for the treatment (or prevention) of ZIKV infections are currently unavailable. Repurposing clinically approved drugs could be an effective approach to quickly respond to an emergency outbreak of ZIKV infections. The well-established safety profiles and optimal dosage of these clinically approved drugs could provide an economical, safe, and efficacious approach to address ZIKV infections. This review focuses on the recent research and development of agents against ZIKV infection by repurposing clinical drugs. Their characteristics, targets, and potential use in anti-ZIKV therapy are presented. This review provides an update and some successful strategies in the search for anti-ZIKV agents are given.
Adult ; Animals ; Drug Repositioning ; Humans ; Infant, Newborn ; Microcephaly ; Pharmaceutical Preparations ; Zika Virus ; Zika Virus Infection/prevention & control*