Arrhythmias, Cardiac ; pathology ; physiopathology ; Humans ; Purkinje Fibers

Since haemoplasmodium is not detected for screening in blood donor,morbidity of transfusion-associated malaria increased rapidly in China recently and became an important problem of public health.It is necessary to emphasize donor screening for malaria.Hematology analyzer with MAPSS is recommended to screen malaria.Flow cytometry and immunochromatography test(ICT)can also screen malaria effectively.For the suspicious samples with haemoplasmodium,it is necessary to be reexamined by standard microscopic examination or polymerase chain reaction(PCR).

Humans ; Leukemia, Lymphocytic, Chronic, B-Cell ; Societies, Medical ; United States

Adult ; Bone Neoplasms ; diagnosis ; surgery ; Cervical Vertebrae ; Humans ; Male ; Osteochondroma ; diagnosis ; surgery

Animals ; Chronic Disease ; Heart Failure ; drug therapy ; Humans ; Hydroxymethylglutaryl-CoA Reductase Inhibitors ; adverse effects ; therapeutic use

Objective By surface-enhanced laser desorption / ionization time-of-flight mass spectrometry(SELDI-TOF-MS), the serum pmteomic fingerprints related with the changing of malignant tumor patients' serum glucose after chemotherapy was selected and constructed as an predictive model. Methods By SELDI-TOF-MS, the serum of 182 malignant tumor patients who had received chemotherapy were tested, and the pmteomic fingerprints were received. After 2 years follow-up, all the patients were divided into 3 groups: the euglycemia group(136 people), the carbohydrate tolerance abnormality group(27 people), and the diabetes mellitus group (19 people). The proteomic fingerprints were analyzed by Biomarker Wizard Software and the idio-proteomic fingerprint of protective models were constructed by BPS (biomarker pattern software). Results The diagnosis model composed with 2 proteins (M/Z values were 5298 and 9608) could classify the carbohydrate tolerance abnormality group, and the diabetes mellitus group correctly. In the test model, the sensitivity and specificity were 81.48 %(22/27) and 100.00 %(17/17) respectively, the accuracy was 88.64 % (39/44). The diagnosis model composed with 3 proteins (M/Z values were 10324, 2761 and 4084) could classify the diabetes mellitus group and the euglycemia group correctly. In the test model, the sensitivity and specificity were 62.35 %(53/85) and 88.24 %(15/17) respectively, the accuracy was 66.67 %(68/102). The diagnosis model composed with 6 proteins (M/Z values were 5895,6010,6099,3930,5430 and 2495) could classify the diabetes mellitus group and the the carbohydrate tolerance abnormality group correctly. In the test model, the sensitivity and specificity were 77.65 %(66/85) and 96.30 %(26/27) respectively, the accuracy was 82.14 %(92/112). Conclusion SELDI-TOF-MS could be utilized to analyze protein profiling in screening serum glucose changing-related biomarkers and developing diagnostic and predictive patterns, and the developed patterns may be used to predict the changing of serum glucose after chemotherapy in malignant tumor patients.

Objective To evaluate the hematologic effects of linezolid in advanced aged patients with hospital onset of infection.Methods The clinical characteristics and complete blood routine examination results of 45 elderly patients aged(92.9±5.2)years treated with linezolid over 600 mg of lowest dose everyday for more than 72 hours were retrospectively analyzed.Results Among the 45patients,20 patients(44.4％)had substantially lower platelet,6 patients(13.3％)lower haemoglobin,1 patient(2.2％)lower absolute neutrophil,and the linezolid treatment in 19 patients were discontinued due to hematological system adverse reactions.The significant drop in platelet occurred at(10.2±3.3)days after treatment.The significant drop in hemoglobin occurred at(9.8±3.0)d after treatment.The lowest count of platelet and hemoglobin took place at(13.1±3.9)d and (10.5±3.5)d after treatment,respectively.The drops in platelet and hemoglobin of some patients took place after cease of treatment.Stepwise logistic regression analysis revealed that treatment duration(OR =1.407,P＜0.05)and creatinine clearance rate(OR=0.732,P＜0.05)were the risk factors for thrombocytopenia.Conclusions The elderly patients using linezolid more likely suffer from adverse reactions of hematological system,particularly thrombocytopeniawhich is significantly more common in patients with renal insufficiency and prolongation of treatment time.The blood routine examination should be monitored closely when elderly patients are treated with linezolid.

Objective:For clinical interventional procedures in surgery,blind spot, as well as minimally invasive surgery more precise surgical navigation requirements, this paper explores the use of a new generation of Aurora electromagnetic tracking technology assisted surgical navigation through free combination with a variety of surgical instruments, surgical further addressed various problems encountered. Methods:Aurora electromagnetic tracking system used with metal-containing material that matches the medical surgical environment, the use of electromagnetic tracking system comes with control software, the use of which is equipped with the SDK (secondary development package), I use a set of VC programming language suitable for clinical surgical navigation, real-time display system, and in our hospital different medical surgical navigation application performance test and comparative experiments measured the accuracy of the tracking system, the statistics of various surgical instruments corresponding to different surgical applications. Results:By author in Jingzhou City, First People's Hospital conducted a series of clinical application, verify the advantages of the system, allows doctors during surgery even more powerful, fully reflects the Aurora electromagnetic tracking system, superior performance, as well as in the medical surgical navigation important far-reaching significance. Conclusion:By using a variety of surgical instruments and medical surgical navigation in different experiments, we found that Aurora electromagnetic tracking system surgery centimeter-level positioning accuracy can be changed from the millimeter level, navigation accuracy average of less than 2 mm, and is available for user secondary development package, free easy programming operation, combined with surgical instruments and flexible, easy to implement intelligent navigation surgery. For clinical work provides an updated, more broad application space, enhancing the overall surgical navigation accuracy and controllability.

OBJECTIVE:To determinate the dissolution of Compound Flavone capsules by HPLC.METHODS:Nova-Pak C18(250mm? 4.0mm,5? m) column was used with column at room temperature.The mobile phase consisted of methanol-0.4% H3PO4(50:50) at a flow rate of 1.0mL? min-1.The detective wavelength was 360nm.The dissolution of the Compound Flavone capsules was determined by basket stirring technique with 0.1mol? L-1 hydrochloric acid as dissolvent at a speed of 100r? min-1.RESULTS:The cumulative dissolution rate of Compound Flavone capsules was above 80% at 30 minutes.The linear range of Quercetin was 0.065 84～ 0.658 4?g(r=0.999 9).The average recovery was 99.35%,RSD=0.92%(n=6).CONCLUSION:The method is simple,accurate and reproducible,and suitable for the guality control of Compound Flavone capsules.

Objective To investigate the incidence of candidal colonization and to analyze its risk factors in patients with connective tissue diseases (CTDs). Methods Throat swabs, midstream urine and anal swabs from 153 patients with CTDs and 63 healthy volunteers were collected for fungus culture. The logistic regression was completed via the software package SPSS 11.0. Results The incidence rate of candidal colonization was higher in the patients than that in the healthy controls (35.29% vs 7.59%). Of all the isolated fungus strains, the proportion of Candida albicans was the highest. The logistic regression analysis revealed that decrease of blood erythrocytes, increase of urine protein, decrease of complements such as CH50, C3 and C4 in serum, high daily dosage of corticosteroids and broad-spectrum antibiotic administration all significantly increased the risk of candidal colonization (all P 0.05). Conclusions The incidence rate of candidal colonization in patients with CTDs is higher than that in healthy controls. Avoiding the risk factors will help to decrease the incidence of candidal colonization in CTDs.