1.Comparation on effect of two kinds operation in treatment of recurrent pterygium with symblepharon
International Eye Science 2014;(12):2286-2288
AlM: To analyze the effect of corneal limbus stem cell transplantation and amniotic membrane transplantation in treatment of recurrent pterygium with symblepharon.
METHODS:Totally 80 cases of patients with recurrent pterygium and symblepharon were randomly divided into pterygium resection combined with limbal stem cell transplantation group and pterygium excision with amniotic membrane transplantation group, 40 cases in each group. All patients were followed up for 6mo after the operation, the recurrence rate of pterygium and symblepharon were observed.
RESULTS:There were 2 cases of recurrence in the group of corneal limbus stem cell transplantation (5%), 8 cases of recurrence in the group of amniotic membrane transplantation (20%), with statistical difference between two groups (P<0. 05).
CONCLUSlON:Two kinds operation could significantly reduce the recurrence rate of recurrent pterygium, but the corneal limbus stem cell transplantation group has the lower recurrence rate.
2.Analysis of External Quality Assessment Results for Blood Cytomorphology from 2011 to 2013 in Shaanxi Province
Xiaojuan XIE ; Xucang WEI ; Ping ZHOU ; Liqiang WEI
Journal of Modern Laboratory Medicine 2014;(5):154-157
Objective To analyse the problems presented in external quality assessment (EQA)for blood cytomorphology and propose measures for improvement.Methods Statistical analysis was performed on EQA results for blood cytomorphology from 2011 to 2013 in Shaanxi Province,including general information of participant laboratories,coincident rate,and reported incorrect results.EQA for blood cytomorphology was held two times every year in Shaanxi province,with ten pictures inclu-ding bone marrow and peripheral blood smear each time.The compact disc including twenty pictures was given to partici-pants by EMS.Participants reported two EQA results in April and September each year.The center statisticed the EQA re-sults and provided the EQA reports to every participants in June and November.Results Participating laboratories increased from 76 in 2011 to 163 in 2013.The ratio of laboratories with the coincidence rate≥80% was 80%,47%,44%,55%,77%and 96% respectively.The number for single cell with the coincidence rate≥80% was 38.The coincidence rate of peripheral blood cells was higher than that of the bone marrow on the whole.Causes of incorrect results included cell lines’misclassifi-cation,growth stage’s misclassification,insufficient identification of abnormal cytomorphology,and so on.Conclusion The identification of blood cytomorphology was unbalanced in different leveled hospitals in Shaanxi Province.To develop EQA of blood cytomorphology definitely has a positive role in improving the experimenters’skill of identifying cytomorphology.
4.Practice and experiences on clinical pharmacy of TCM
Tanjun WEI ; Xiaoqun XIE ; Shuangshuang WANG ; Ping ZHOU ; Yi WANG
International Journal of Traditional Chinese Medicine 2014;(8):741-743
By retrospective analysis method, the paper was summarized experience of clinical pharmacy of TCM. The purpose of the paper was given location of clinical pharmacists in Traditional Chinese Medicine(TCM), to clear their main status and functions, seek methods and ways to solve the problems in the process of work. The work was proceeding orderly. Good results have been achieved. The clinical pharmacists of TCM participate in clinical rational use of drugs to change the service mode ofindirect service for the patients in the past with the patients, doctors and nurses so as to make better service for people's life and health of Chinese medicine.
5.The role of vitamin D in bone metabolism of ankylosing spondylitis
Jianli XIE ; Ping WEI ; Junxiang WANG ; Hanying CHEN ; Jinghong MENG
Chinese Journal of Rheumatology 2013;17(11):771-774
Objective The goal of this study was to investigate the correlation between the level of serum vitamin D and bone metabolism and disease activity in ankylosing spondylitis(AS) patients,and thus to explore the role of vitamin D in bone metabolism in AS patients.Methods The serum levels of BALP,TRACP-5b,25-(OH)D3 and blood lymphocytes VDR of 80 AS patients were measured by enzyme-linked immunosorbent assay (ELISA) and compared with those of the control group.Bone mineral density (BMD)was measured by dual-energy X-ray absorptiometry (DEXA).The ESR and CRP level of AS patients were also measured.The correlation between those parameters was analyzed and evaluated.Patients were divided into normal,insufficient and deficient subgroups according to the serum 25-(OH)D3 levels for further comparison.Indepondent saimple t test,t'test andx2 test were used for statistical analysis.Results The 25-(OH)D3 of AS patients [(11.9±2.7) μg/L] was significantly lower than that of the control groups [(22.3±7.9) μg/L] (P<0.05),while the serum levels of BALP [(3.9±2.7) μg/L] and TRACP-5b [(46±25) ng/L] of AS patients were significantly higher than those of the control group [(2.4±1.0) μg/L] (P<0.05).According to linear correlation analysis,25-(OH)D3 was negatively correlated with CRP (r=0.324,P=0.003).The ESR,BALP,TRACP-5b in the deficient subgroup were higher than those in the normal and insufficient subgroups(P<0.05).Conclusion The plasma 25-(OH)D3 may decrease in AS patients,and this may activate bone metabolism,results in increased morbidity of osteoporo-sis,and negatively affect disease activity.
6.Therapeutic Observation of Suspended Moxibustion plus Chinese Medication for Icteric Hepatitis
Yanfeng XIE ; Bingquan MO ; Ping GUI ; Wenzhu WEI
Shanghai Journal of Acupuncture and Moxibustion 2015;(7):639-641
Objective To observe the clinical efficacy of suspended moxibustion plus Chinese medication in treating icteric hepatitis. Method Ninety-two patients with icteric hepatitis were randomized into a treatment group and a control group, 46 in each group. The treatment group was intervened by suspended moxibustion plus oral administration of Chinese medication for supplementing yang, tonifying spleen, expelling dampness, and activating blood circulation;the control group was intervened only by the same Chinese medication. The symptom and sign score, and liver function items including TBil and ALT were observed, and the clinical efficacy was compared. Result The symptom and sign scores were significantly changed in both groups after intervention (P<0.05). After intervention, there was a significant difference in comparing the symptom and sign score between the two groups (P<0.05). The TBil and ALT levels were significantly changed after intervention in both groups (P<0.05). The recovery rate and total effective rate were respectively 87.0%and 95.7%in the treatment group versus 76.1%and 82.6%in the control group, and the inter-group differences were statistically significant (P<0.05). Conclusion Suspended moxibustion plus Chinese medication is an effective method in treating icteric hepatitis.
7.Ubiquitin ligase Smurf1 promotes Smurf2 neddylation modification
Chao LIU ; Haiwen LI ; Rongfei WEI ; Ping XIE ; Lingqiang ZHANG
Military Medical Sciences 2017;41(2):86-89
Objective To investigate the mechanism of Smad ubiquitination-related factor 2 (Smurf2)neddylation. Methods The Smurf2 protein level was tested by overexpression of Nedd8,while the method of immunoprecipitation(IP) and Western blotting were used to analyz Smurf2-Nedd8 modification.The GST-pulldown experiment was conducted to prove protein interactions.The protein was obtained by grinding mouse tissue and Western blotting was used to test the protein expression level.Results Over expression of Nedd8 could lead to the down regulation of the Smurf2′s protein level.Smurf1 and Smurf2 could interact in the GST-pulldown experiment. Smurf1 could enhance Smurf2-Nedd8 modification.The Smurf2′s protein level was up-regulated in mouse tissue of Smurf1 C426A.Conclusion There is some relationship and also difference between Smurf1 and Smurf2.Smurf1 can enhance Smurf2-Nedd8 modification.
8.A New Diol from Dimocarpus longan Seeds
Gongming ZHENG ; Liangxiong XU ; Haihui XIE ; Ping WU ; Xiaoyi WEI
Chinese Herbal Medicines 2011;03(1):7-8
Objective To investigate the chemical constituents of Dimocarpus longan seeds in Sapindaceae.Methods The chemical constituents were isolated from the ethanol extract of D.longan seeds by silica gel column chromatography.Their structures were identified on the basis of physical and chemical properties and spectral analysis.Results One compound was isolated and identified as 2-methyl-1,10-undecanediol,named longandiol(1).Conclusion Compound 1 is a new compound.
9.Levels of IL-18, IL-16, IL-8, eotaxin and the chymase activity in the sputum of asthmatics
Hua XIE ; Shaoheng HE ; Jifu WEI ; Li SUN ; Ping CHEN
Chinese Journal of Pathophysiology 2000;0(11):-
AIM: To investigate the levels of IL-18, IL-16, IL-8, eotaxin and the chymase activity in the sputum of asthmatics. METHODS: IL-18, IL-16, IL-8 and eotaxin levels were detected with sandwich ELISA procedures and chymase activity was determined spectrophotometrically (410 nm) by the rate of hydrolysis of N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide (SAAPP). RESULTS: The specific chymase activities in the severe and moderate asthmatics were higher than that in controls. Native protease inhibitors ?_1-antitrypsin (?_1-AT) and soybean trypsin inhibitor (SBTI) inhibited 71.9% and 72.1% enzymatic chymase activity, respectively. The levels of IL-18, IL-16, IL-8 and eotaxin were significantly elevated in the sputum of patients with acute asthma. There were correlations between the levels of IL-8 and IL-16 (r=0.55, P
10.Effects of Saikosaponin a on TNF-? release and its receptor expression in cultured hippocampal astrocytes induced by pentylenetetrazol
Wei XIE ; Ping KANG ; Zuowen ZHANG ; Linlin ZHU ; Yong BAO
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(07):-
Objective:To study e ects of Saikosaponin a(SSa) on tumour necrosis factor-alpha(TNF-?) release and its receptor expression in cultured hippocampal astrocytes induced by pentylenetetrazol(PTZ).Methods:The in vitro cultured primary hippocampal astrocytes were randomly divided into control group(group A),PTZ-induced group(group B)PTZ10mmol/L+SSa groups(group C and group D,the SSa concentrations were 1.25mg/L and 0.625mg/L respectively).The extracellular uid TNF-? level and the expression of tumour necrosis factor receptor type 1(TNFR1) in hippocampal astrocytes were respectively detected by ELISA and Western-blot after PTZ-induced 2 hours.Results:the TNF-? level and TNFR1 expression of group B were signi cantly higher than that of group A,group C and group D(P