1.How to improve the level of endoscopic diagnosis and treatment of early gastrointestinal cancer: private opinions.
Chinese Journal of Gastrointestinal Surgery 2012;15(7):649-653
At present, the level of diagnosis and treatment of early gastrointestinal cancer has a larger gap between China and developed countries. In recent years, the advent of new endoscopic imaging techniques has improved the detection rate of early gastrointestinal cancer. Along with this, endoscopic submucosal dissection(ESD) enables radical resection for early cancer. In this article, we discuss how to improve the level of endoscopic diagnosis and treatment of early gastrointestinal cancer.
China
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Endoscopy, Digestive System
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Gastrointestinal Neoplasms
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diagnosis
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surgery
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Humans
2.Effects of colon hydrotherapy on lowering body temperature,serum enzymes and inflammatory cytokines of dogs with heat stroke
Xin YAO ; Zitan FENG ; Wei LIU
Medical Journal of Chinese People's Liberation Army 2001;0(09):-
Objective To investigate the effects of colon hydrotherapy on lowering body temperature and serum enzymes as well as inflammatory cytokines of dogs with heat stroke.Methods 14 dogs were randomly devided into 2 groups,7 for each,as colon hydrotherapy group and conventional therapy group.After the reproduction of heat stroke model was finished(core temperature reached 43℃,maintaing for 10min),cooling therapy was commenced on dogs in circumstance with temperature at 23℃ and 65% of humidity.Conventional therapy group was lowered temperature by ice bags placed on conducting arteries,and the colon hydrotherapy group was by colon way therapeutic machine,the temperature of therapeutic liquid was maintained between 37℃~38℃.Cooling was finished while temperature in dogs reduced to 38℃.The periods of time for lowering body temperature in both groups were compared.Alanine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH)and creatine kinase(CK)were determined before heat stroke(0h),and 6h,24h and 48h after heat stroke.Interleukin-1?(IL-1?),Interleukin-6(IL-6)and tumor necrosis factor ?(TNF-?)were determined by radioimmunity assay.Results Body temperature lowering time in dogs with colon hydrotherapy were 92.9?9.5min,which was significantly shorter than that of conventional therapy group(123.4?11.6min,P0.05).IL-1? in colon hydrotherapy group at 6h,24h and 48h after treatment were significantly lower than those in conventional therapy group(P
3. Metabolomics study of Erzhi pills on acute hepatic injury induced by CCl4 in rats
Chinese Pharmaceutical Journal 2013;48(10):808-813
OBJECTIVE: To observe the plasma metabolic changes of rats after acute hepatic injury induced by CCl4 and the regulating action of Erzhi pills (EZW, traditonal Chinese medicines) on abnormal metabolism, and to find the potential biomarkers. And to explore the mechanisms of Erzhi pills in treatment of acute hepatic injury induced by CCl4. METHODS: Endogenous metabolites in plasma were determined with RRLC-Q-TOF/MS. RESULTS: Nine potential metabolic biomarkers were identified qualitatively. Two metabolic pathways were identified using ingenuity pathway analysis (IPA). CONCLUSION: Changed metabolites can be recovered by Erzhi pills in different degrees, and the treatment effect of Erzhi pills may be related with the regulation of two metabolic pathways. Unique pathway analysis may be effective in drug-target identification, which helps to further understand mechanism of drug action, and improve research efficiency.
4. Study on the excretion of schizonepetin in rats
Chinese Pharmaceutical Journal 2012;47(16):1318-1323
OBJECTIVE: To study the excretion profile of schizonepetin in rats. METHODS: An HPLC-UV method was developed and validated for the determination of schizonepetin in urine, feces and bile. Using this method, the excretion features were studied in rats after oral and intravenous administration. RESULTS: There was significant difference in the excretion percentages of schizonepetin between male and female both in rat urine and bile for oral administration and in urine for intravenous administration. No significant difference was observed in the excretion of schizonepetin through feces for the two administration routes. CONCLUSION: The amount of schizonepetin recovered from urine, feces and bile after administration is very low, indicating that schizonepetin is mainly excreted in the form of metabolites. Copyright 2012 by the Chinese Pharmaceutical Association.
5.Expression of osteopontin in local tissues of patients with cervical cancer
Xiaoling SUN ; Weihong YAO ; Qingping WEI ; Yahong FU ; Feng FENG
Cancer Research and Clinic 2010;22(z1):11-13
Objective To detect the expression of osteopontin (OPN) in cervical local tissues of patients with chronic cervicitis, cervical intraepithelial neoplasia (CIN), cervical cancer. To investigate the possibility that OPN is used as tumor marker of cervical cancer. Methods The expression of OPN in 25 cases of chronic cervicitis, 18 cases of CIN and 21 cases of cervical cancer was detected by immuohistochemistry SP method. Results The positive expression rates of OPN in the chronic cervicitis, CIN and the cervical cancer had a rising trend (48 %, 77.78 % and 80.95 %, respectively) and their differences had statistical significant (P <0.05). The OPN expression level in cervical cancer had no statistical correlation with age, tumor size, clinical stage and other clinical pathological features (P >0.05). Although OPN expression level in cervical cancer wasn' t related with tumor clinical stage, positive expression rates of OPN had a gradually increased trend along with the clinical stage. Conclusion High expression of OPN is found in cervical cancer and may be related to the occurrence and development of cervical cancer.
7.Diagnostic significance of urethral sphincter electromyography and external anal sphincter electromyography in patients with multiple system atrophy
Feng QIU ; Xiaokun QI ; Liping LI ; Jianguo LIU ; Wei YAO
Chinese Journal of Neurology 2012;45(3):188-191
Objective To assess the significance of urethral sphincter electromyography (US-EMG) and external anal sphincter electromyography (EAS-EMG) for the diagnosis of multiple system atrophy (MSA).Methods US-EMG and EAS-EMG were performed in 9 patients who were diagnosed as MSA.Duration,motor unit action potentials amplitude,polyphasicity,as well as recruited pattern and amplitude during powerful contraction were recorded and analyzed.Results Among 9 patients who were diagnosed as MSA,7 cases showed neural injury by both US-EMG and EAS-EMG.There was significant difference of electromyographic findings between US-EMG group and EAS-EMG group (average volatility (μV):1063.44 ±499.92 vs 634.89 ±265.07; polyphasic wave:0(0,20% ) vs 57% (28%,63% ) ; t =2.567,P=0.033;t =2.833,P=0.012).Conclusions Although US-EMG may be difficult to perform,US-EMG may have the same specificity as EAS-EMG for the diagnosis of MSA,especially for the diagnosis of MSA patients only with urination disorders,who are involved in Onuf neclear according to some of the abnormal indexes.
8.Effect of methylcobalamin,gliclazide and combined application of both on sciatic nerve morphology and nerve growth factor contents in streptozotocin-induced diabetic rats
Weiyi FENG ; Hongping YAO ; Youxia WEI ; Haiyan DONG
Chinese Pharmacological Bulletin 2003;0(10):-
Aim To investigate the curative effect and the mechanism of methylcobalamin,gliclazide and combined application of both on diabetic peripheral neuropathy(DPN)in streptozotocin-induced diabetic(STZ-D)rats.Methods STZ-D rats were administered with drugs orally for 8 weeks after streptozotocin treatment.Morphologies of sciatic nerve were observed with histological method.Nerve growth factor(NGF)contents in plasma or in sciatic nerve tissues were determined by ELISA kit or immunohistochemistry.Results Morphological degeneration in sciatic nerve,decrease of NGF concertration in plasma,as well as in nerve axis cylinder,in diabetic rats was all ameliorated by methylcobalamin and/or gliclazide.However,no significant differences in morphology and NGF contents were found between single drug treated groups and combined application group.Conclusions The observations suggest that methylcobalamin,gliclazide and combined application are effective in treating DPN in STZ-D rats.No significantly enhanced effect is found in combined application group as compared with methylcobalamin or gliclazide group.
9.The diagnostic value of urethral sphincter electromyography in patients with multiple system atrophy
Xiaokun QI ; Liping LI ; Wei YAO ; Jianguo LIU ; Feng QIU
Chinese Journal of Internal Medicine 2012;(12):975-977
Objective To analyze diagnostic evaluation of urethral sphincter electromyography (US-EMGs) for patients with multiple system atrophy (MSA).Methods Totally 15 patients who were diagnosed as MSA were examined as treatment group while 17 non-MSA patients were examined as controls.US-EMGs were performed in the both groups.Spontaneous activities when relax,parameters of motor unit potentials(MUPs) mean duration and amplitude,percentage of polyphasic ware,satellite potential,recruitment potentials and amplitude when strong contraction were recorded and analyzed.Results USEMGs changes of various abnormalities were found in 13 cases (86.7%) in MSA group.There were significant differences of electromyographic findings between the MSA group and control group including MUPs mean duration[(12.79 ±3.18)ms vs (9.49 ± 1.51)ms] and amplitude[(828.53 ±459.89) μV vs (378.76 ± 152.26) μV] as well as recruitment potentials [(11.47 ± 21.55) % vs (8.23 ± 10.74) %] and amplitude [(2.19 ± 1.24) mV vs (0.75 ± 0.42) mV] when strong contraction (all P values < 0.05).Conclusions There is certain value of US-EMGs for the diagnosis of MSA.It could be used as a routine electrophysiological method for the patients who are suspected of MSA.It could be a supplement of externalanal sphincter electromyography.
10.Medication Analysis for One Patient with Severe Ulcerative Colitis
Tongfei YANG ; Weiyi FENG ; Youxia WEI ; Maoyi WANG ; Hongping YAO
China Pharmacist 2015;18(10):1773-1776
Objective:To analyze the medication of one patient with ulcerative colitis to provide pharmaceutical care and support for rational drug use in patients with ulcerative colitis. Methods:During the treatment of the patient with severe ulcerative colitis, clin-ical pharmacists analyzed the drugs used by the patient and provided pharmaceutical care for doctors and the patient according to the ex-amination and diagnosis of the patient. Results:The compliance, therapeutic effect and medication safety of the patient were all im-proved by giving clinical drug rationalization suggestions and targeted medication monitoring and education, which fully embodied the necessity of work of clinical pharmacists in the medication of patients. Conclusion:Through case analysis, clinical thinking of clinical pharmacists can be developed to promote rational drug use, avoid adverse drug reactions and achieve optimal effect of drug treatment.