Objective To investigate the role of compound traditional Chinese medicine Yiqilin in the regulation of immune-related genes expression in spontaneously hypertensive rats(SHR).Methods Ten SHR were randomly divided into test group(fed with Yiqilin and ordinary food at the ratio of 1:9 for 6 months,n=5)and control group(fed with ordinary food for 6 months,n=5).Total RNA was extracted from myocardial tissues of SHR after 6 months,and gene chip hybridization technique was employed to analyse the expression of immune-regulated genes of myocardial tissues in two groups.The genes with differentiation expression were screened,and were verified by Real-time PCR.Results The expression of 32 immune-related genes were up-regulated in the test group,including interleukin(IL)6 receptor,IL-6 signal transduction factor,chemotatic factor,antigen presenting molecule,antibody receptor,heat shock protein(Hsp), etc.No immune-related gene was down-regulated in test group.The results of 2 immune-related genes(Hsp 105 and Hsp 90)detected by Real-time PCR were consistent with those by gene chip hybridization.Conclusion Part of the immune-related genes in myocardial tissues in SHR may be up-regulated by Yiqiling,which indicates that Yiqiling may play a role in the regulation of expression of immune-related genes in SHR.

The changes in cell adhesion molecule CD15 and PECAM-1 in grafted renal tissue from 18 cases of acute allograft rejection were studied by immunohistochemistry techniques (SP method ) and computerized imaging analysis system. The results showed that: (1) CD15 positive expression level was higher in acute allograft rejection than that in normal renal in tuberular cells.(2) PECAM-1 expression level was lower in glomerular endothelial cells, but higher in tuberular cells than those in normal renal tissue during acute allograft rejection. The above findings suggested that the detection of CD15 and PECAM-1 in the grafted kidney might have some values in the diagnosis of acute allograft rejection.

· AlM: To explore the correlation of center retinal thickness and axial length in non-proliferative diabetic retinopathy ( DR) .
· METHODS:A total of 80 cases ( 155 eyes ) of non-proliferative DR patients were divided into three groups according to the axial length, 22~24mm of 27 cases (51 eyes) were normal group, 24 ~26mm of 28 cases ( 55 eyes) were long axis group, >26mm of 25 cases ( 49 eyes) were super long axis group.The center retinal thickness and ocular axial length of three groups were measured and the correlation of center retinal thickness and axial length were analyzed.
· RESULTS:Among the inner ring and outer ring, the center retinal thickness of upper and lower, bitamporal and nasal side of super long axis group was thicker than that in normal group, with statistically significant difference ( P<0.05 ); Among the inner ring and outer ring, the center retinal thickness of upper and lower, bitamporal and nasal side of super long axis group was thicker than that in long axis group, with statistically significant difference ( P<0.05); The DR central macular of retinal thickness was no correlation of the axial length ( P >0.05 ), but retinal thickness get thinning with increased of axial length on the inner ring and outer ring area, showed a negative correlation ( rinner ring =-0.63, router ring=-0.67, P<0.05).
·CONCLUSlON:Center retinal thickness and ocular axial length in non -proliferative DR patients, and center retinal thickness of super long axis patients are thinning obviously.

Objective:To evaluate the therapeutic effects of capsule of Biqi Capsule and VitB on lubar intervertebral disc protrusion(LIDP).Methods:78 Patients with LIDP were divided randomly into 2 groups:treatment and control group.39 Patients in treatment group were treated with Biqi Capsule and FENG's bone-setting manipulation,39 patients in control group were treated with vitamine B and FENG's bone-setting manipulation.All patients were followed up for 1 month after treatment for 2 weeks and therapeutic effects were evaluated.Results:In treatment group,24 patients were cured,6 patients had remarkable effects,5 effective,4 ineffective.In control group,the data were 16,8,8,7 correspondly.The therapeutic effects in treatment group were obviously better than those of control group(P

Neuro-ophthalmology,as an interdisciplinary,covers ophthalmology,neurology and neurosurgery.In China,the development of neuro-ophthalmology has just started,therefore how to train neuro-ophthalmological specialists in China is a problen.This paper analyzed the medical education and current situation of neuro-ophthalmology of China and America,discussed the differences between them,and then put forward some problems existing in the neurological ophthalmology physician training and solutions,aiming at improving the specialist physician train program of Neuro-ophthalmology by learing from foreign medical education development experience,and promoting the development of neuro-ophthalmology in China.

Objective To explore the microcosmic mechanism of pingyangmycin treating infantile proliferating capillary hemangiomas so as to find the optimal method for hemangiomas′ treatment.Methods Sixty samples of infantile proliferating hemangiomas were divided into control group(30 cases,aged from 2 days to 6 months) and experimental group(30 cases,aged from 2 to 6 months).Pingyangmycin was made into emulsion and smeared on the surfaces of the leision in experimental group with 3 times every day as well as only matrix in control group.The specimens were resected on d7,then made into pathological slices and electron microscope slices in order to observe the cells microcosmic structure changes and ultrastructure changes.Furthermore,the apoptotic indexes of two groups were detected by the molecular biology method(TUNEL test).Results The number of apoptotic cells were lower in control group(apoptotic index 18.87?13.67)but higher apparently in experimental group(apoptotic index 29.52?15.33).The difference between two groups was significant(t=2.842 P

Objective In order to explore the action of IL-1? involved in epileptogensis,the changes of Gs? expression were studied in hippocampus of epileptic rats induced by sodium glutamate. Methods Immunohistochemical staining and animal behaviours were observed during the experiments.Healthy adult SD rats were divided randomly into five groups:1.Control.2.GluNa group.3.IL-1?+GluNa group.4.rhIL-1ra+IL-1?+GluNa group.5.D-AP-5+IL-1?+GluNa group. Results The changes of behaviours showed that latent period of seizure in IL-1?+GluNa group was reduced remarkly compared with that of other groups;the degrees of seizure were more serious than that of other groups.The results of immuohistochemistry showed that immunoreaction of Gs? was obviously enhanced in hippocampal CA1,CA3 areas and dentate gyrus of IL-1?+GluNa group compared with other groups.Conclusion IL-1? may play an important role in medicating pathologenesis procession of epilepsy induced by glutamate and make effect by stimulating overexpression of Gs protein.

OBJECTIVE To analyze the pathogens distribution and the antibiotic resistant characters.METHODS Sputum culture was performed in 267 cases who suffered from lower respiratory tract infection in emergency ward from Jan 2004 to Jun 2007.The results of 183 pathogens and drug sensitivity were analyzed.RESULTS The detected 183 strains of pathogens showed that Gram-negative bacilli accounted for 77.1%(Klebsiella pneumoniae was in the first place) and Gram-positive cocci were for 22.9%(Enterococcus faecium and Staphylococcus aureus were in the first places),the total drug resistance rate of Gram-negative bacilli to gentamicin,cefazolin,SMZ/TMP,piperacillin,ciprofloxacin and aztreonam had highly reached 60.5-70%,however to meropenem,cefepime,amikacin,imipenem and Sulperazone was 32.5-38.6%.Gram-positive cocci appeared generally higher multidrug resistant,the drug resistance rate of Gram-positive cocci to penicillins and erythromycin was all 100%,but the resistantce of Gram-positive cocci to vancomycin hadn′t been found.CONCLUSIONS Gram-negative bacilli are major pathogens in lower respiratory tract infection of emergency ward,K.pneumoniae is in the first place of Gram-negative bacilli and E.faecium and S.aureus are in the first places of Gram-positve cocci,both of them appearing highly multidrug resistant,analysis of pathogens and drug sensitivity are necessary to rational antibiotics usage,and the immunological protection should be enhanced.

Adult ; Air Pollution, Indoor ; adverse effects ; Benzene ; adverse effects ; Control Groups ; Female ; Humans ; Leukocyte Count ; Male ; Middle Aged ; Occupational Exposure ; adverse effects ; Paint

A high Streptolydigin-producing strain Streptomyces lydicus AS 4.2501-L8 with genetic stability was obtained from the original strain S. lydicus AS 4.2501-P28 by mutagenesis with UV and acridine orange, followed by resistance selection. Its yield of Streptolydigin was 177.0 ?g/mL, 96.2% higher than the original strain.