Acute Disease ; Adolescent ; Adult ; Child ; Child, Preschool ; Female ; Humans ; Male ; Middle Aged ; Mushroom Poisoning ; diagnostic imaging ; Ultrasonography

Objective To analyze domestic and foreign papers on H5N1 virus research using literature metrology and visualized knowledge mapping.Methods Pajek, Bibexcel,and VoSviewer software were used based on Web of Knowledge platform.Results Study on H5N1 virus has been a research focus wide since 2005 and a series of institutions and investi-gators have appeared, with US being the leader in this area and followed by China.The most popular topics of studies on H5N1 included its virology, epidemiology, basic research, anti-virus drugs and vaccines.Conclusion Studies on H5N1 have received more attention, which suggests that more efforts have to be made in China in terms of communication and co-operation with key institutions and famous researchers so as to achieve more success in key areas.

Objective To identify research fronts of synthetic biology by mapping knowledge domains .Methods The research articles were clustered by co-cited analysis and bibliographic coupling while research domains of synthetic biology were mapped using software VOSviewer .Results There were seven research clusters by co-cited analysis and 32 clusters by bibliographic coupling .The articles published in the last two years have changed the structure of research domains .Con-clusion Bibliographic coupling is better at recognizing research fronts .Synthetic bioengineering , RNA expression , biology bone, biology library,and biology parts are research fronts of synthetic biology .

Nuclear medical emergency rescue , as an important task for the military , has become a priority in many countries.The U.S.military has accumulated rich experience in the development of nuclear medical emergency rescue forces.This paper analyzes the legal status , roles, responsibilities and tasks of the U .S.military during nuclear emergency rescue as well as the characteristics and current status of nuclear emergency rescue forces , hoping to provide some refer-ences for development of our military nuclear medical emergency rescue forces .

Objective To clarify the cellular localization of triptolide and to explore its in-cell action sites.Methods 4-(Bro-momethyl)-7-methoxycoumarin was employed to label triptolide,then labelled triptolide was incubated with human hepatoma carci-noma cells.Subsequently,incubated cells were subjected to stain with fluorescent dye DiI or PI,which were specific to cytoplasmic membrane system and nucleus,respectively.Results Compared with the non-triptolide control,coumarin labelled triptolide shown a light blue fluorescence under UV excitation;Co-localization with DiI showed that triptolide exist in cytoplasm and(or)on cell mem-brane;Co-localization with PI showed that triptolide located in cell nucleus.Moreover,microscopic observation indicated that the fluorescence intensity in nucleus was denser than that in cytoplasm.Conclusion The presnt study demonstrate that triptolide main-ly act in nucleus,followed by acting in cytoplasm and(or)on cell membrane.

Animals ; Gene Targeting ; instrumentation ; Genetic Vectors ; chemistry ; genetics ; metabolism ; Humans ; Macromolecular Substances ; chemistry ; Viruses ; chemistry ; genetics ; metabolism

A NTC thermistor-based wearable body temperature sensor was designed. This paper described the design principles and realization method of the NTC-based body temperature sensor. In this paper the temperature measurement error sources of the body temperature sensor were analyzed in detail. The automatic measurement and calibration method of ADC error was given. The results showed that the measurement accuracy of calibrated body temperature sensor is better than ± 0.04 degrees C. The temperature sensor has high accuracy, small size and low power consumption advantages.
Body Temperature ; Calibration ; Humans ; Monitoring, Physiologic ; methods

OBJECTIVE: To investigate the effect of trifluoro-icaritin (ICTF) on myocardial ischemia/reperfusion injury (MI/RI) in rats and to explore whether it plays a role in regulating autophagy through the serine/threonine kinase/mammalian target of rapamycin (Akt/mTOR) signaling pathway. METHODS: Male SD rats were ligated for 45 min and reperfused for 60 min to establish an MI/RI model. The rats were divided into sham, MI/RI model and model+ICTF 0.5, 1.0 and 2.0 mg·kg-1groups. II lead electrocardiogram (ECG) in T wave and ST segment changes were recorded. The area of myocardial infarction was determined by TTC. The protein levels and phosphorylation levels of microtubule-associated protein 2/1 light chain 3 (LC3 II/LC3 I), Beclin-1, Akt and mTOR in myocardial tissues were detected by Western blotting. The level of LC3 in myocardial tissues was detected by immunofluorescence test. RESULTS: The ECG showed that the T wave (P<0.05) and ST segment (P<0.01) of the model group were significantly higher than those of the sham group after 60 min of reperfusion, while the T wave (P<0.05) and ST segment (P<0.01) of the ICTF 1.0 mg·kg-1group were obviously lower than those of the model group. TTC staining of heart sections showed that the area of myocardial infarction in the model group was larger than in the sham group (P<0.01), while that in the ICTF 1.0 mg·kg1group was smaller than in the model group (P<0.01). Western blotting results showed that compared with the sham group, the ratios of LC3 II/LC3 I (P<0.01) and p-Beclin-1/Beclin-1 (P<0.05) in the model group were significantly increased, while Akt (P<0.01) and mTOR (P<0.05) were decreased. In addition, compared with the model group, the ratio of LC3 II/LC3 I (P<0.01) and p-Beclin-1/Beclin-1 (P<0.01) in the ICTF 1.0 mg·kg-1group was reduced, and the phosphorylation levels of Akt (P<0.01) and mTOR (P<0.01) were increased. Immunofluorescence results of frozen sections of myocardial tissues showed that LC3 protein expression increased in the model group compared with the sham group (P<0.01), but decreased in the ICTF 1.0 mg·kg-1group compared with the model group (P<0.01). CONCLUSION: ICTF has a protective effect on myocardial ischemia/reperfusion injury in rats, and its mechanism may be related to the regulation of Akt/mTOR signaling pathway to inhibit excessive autophagy.

Abstract: Objective　To analyze the molecular serotyping and drug resistance of foodborne Listeria monocytogenes (LM)isolates from the food monitoring network of Liaoning Province, and to provide a reference for identifying and tracing the outbreaks of foodborne diseases. Methods　The Listeria monocytogenes isolates were identified, biochemical typing was conducted by VITEK biochemical identification and PCR amplification techniques were applied for molecular serotyping of 67 strains of Listeria monocytogenes detected from 2 797 food samples collected from 15 monitoring sites across the province during 2021-2022. Verification was performed according to the instructions of the serodiagnosis manual. The sensitivity of the strains to ampicillin, penicillin, meropenem, cotrimoxazole, and erythromycin was determined by broth dilution method. Results　In food from Liaoning Province, the molecular serotypes of Listeria monocytogenes carried were identified as 1/2a(3a), 1/2b(3b, 7), 1/2c(3c), and 4b (4d, 4e), with the predominant serotypes being 1/2a accounting for 62.7% (42/67), and 1/2b accounting for 28.4% (19/67). Among them, the 4b strain was isolated in 3 strains, representing 4.5%. The dominant strains of Listeria monocytogenes in 2021 and 2022 in Liaoning Province were 1/2a(3a) and 1/2b(3b, 7), with the most diverse types carried by meat and meat products. Double resistance to cotrimoxazole and erythromycin accounted for 11.9% (8/67), while resistance to only one cotrimoxazole accounted for 9.0% (6/67). Conclusions　The presence of pathogenic serotypes 1/2a(3a), 1/2b(3b, 7), 1/2c(3c), and 4b (4d, 4e) in food in Liaoning Province and the emergence of strains with one or more drug resistances indicate the existence of food safety issues caused by Listeria monocytogenes, posing a potential risk of listeriosis. There is a need to optimize source tracing methods and strengthen the confirmation of Listeria monocytogenes outbreaks.

The outbreak of Ebola hemorrhagic fever in West Africa in February 2014 is the worst one in the history of the disease.Medicines and vaccines for Ebola have attracted special attention .This study utilizes Cortellis database of Thom-son Reuters and knowledge about Ebola virus to analyze current R&D of medicines and vaccines for Ebola in order to offer references for research and development .Study findings shows that there are mainly 5 important categories:vaccines, neu-tralizing antibodies , small-molecule anti-Ebola drugs , RNAi drugs and nucleotide drugs .In each category , there are prom-ising products under development that are worthy of attention .