Objective To evaluate the role of PI3K-Akt-HIF-1α signaling pathway in dexmedetomidine-induced reduction of damage to the unventilated lung during one-lung ventilation (OLV) in rats.Methods Forty adult male Sprague-Dawley rats,weighing 250-350 g,were randomly divided into 4 groups (n=10 each) using a random number table:two-lung ventilation TLV) group,OLV + normal saline group (group OLV),OLV + dexmedetomidine group (group D),and OLV + dexmedetomidine +LY294002 group (group DL).Bilateral lungs were ventilated for 2.5 h in group TLV,and the right lung was ventilated for 2 h followed by 0.5 h TLV in the other groups.The rats were sacrificed,the left lung was removed for determination of wet to dry lung weight ratio (W/D ratio),and expression of phosphorylated Akt (p-Akt) and hypoxia-inducible factor-1 alpha (HIF-1α) (by Western blot).Results Compared with group TLV,W/D ratio was significantly increased,the expression of p-Akt was down-regulated,and the expression of HIF-1α was up-regulated in the other three groups (P＜0.05).Compared with group OLV,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-lα was up-regulated in D and DL groups (P＜0.05).Compared with group DL,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-1α was up-regulated in group D (P＜0.05).Conclusion PI3K-Akt-HIF-1α signaling pathway is involved in dexmedetomidine-induced reduction of damage to the unventilated lung during OLV in rats.

The deposition of amyloid β-protein (Aβ is the key pathological basis of cerebral amyloid angiopathy (CAA) and Alzheimer's disease (AD). The current studies emphasis that the stability of internal environment between cells of the neurovascular unit is the key to maintain normal brain function. This article reviews the relationship between the Aβand the neurovascular unit injury, and we expect to provide some new ideas for the clinical prevention and treatment of CAA and AD.

Asia ; epidemiology ; Humans ; Non-alcoholic Fatty Liver Disease ; epidemiology ; Prevalence

DICOM standard is a compulsory international standard for the establishment of Integrated Hospital Information System (IHIS) and Picture Archiving and Communication System (PACS). This paper introduces such information of DICOM3.0 as its generation, components, operational mechanism and application. To understand DICOM standard exactly is helpful for the efficient operation of PACS.

There are many therapeutic drugs for rheumatoid arthritis(RA) treatment,but the pathogenesis of RA have not been fully understood,therefore no curable drug has been found.With increasing knowledge of the inflammatory process,especially in signaling,some drugs modifying disease progress and interfering signaling pathways have been studied.Cytokine inhibitors such as tumor necrosis factor-blocking agents and IL-1 receptor antagonist in clinical trials and in practice have resulted in dramatic improvement in RA disease control.Important molecular mechanisms of actions on total glucosides of paeony(TGP) and Glucosides of Chaenomeles speciosa(GCS) were showed to modulate G protein-AC-cAMP and Ras-MAPKs signaling pathways and to inhibit synoviacytes proliferation.This review focuses on agents which target various cytokines,other inflammatory mediators,antigen of cells,and signaling pathways.

Objective To explore whether microsatellite instability (MSI) is exsist in human gastric carcinoma and precancerous lesion.Methods The MSI was detected in gastric carcinoma and precancerous lesion by tissue laser capture microdissection and denaturing high performance liquid chromatogragh (DHPLC).Results MSI was found in 10.5 ％ intestinal metaplasia,21.12 ％ gastric hyperplasia and 43.75 ％gastric carcinoma respectively.In contrast,there was no microsatellite instability in normol gastric mucosa.It displayed accumulation and alteration from normal to cancerous tissues.Conclusion Pure tumor samples are obtained from tissue laser capture microdissection which could avoid the heteroplasmy of tissue.DHPLC is a high sensitive,fast and semiautomated method for identifying MSI.This experiment showed an increasing tendency of MSI incidence from precancerous lesion to gastric carcinoma.MSI could be used as a marker to predicate the occurrence of gastric carcinoma.

Objective To evaluate the clinical efficacy of Danhong injection combined with edaravone in the treatment of acute ischemic stroke.Methods 90 patients with acute ischemic stroke were randomly divided into control group and observation group,45 cases in each group.The control group was given edaravone treatment on the basis of conventional treatment,the observation group was treated with Danhong injection combined with edaravone treatment,the two groups were treated for 14 days.The changes of main physiological indices,clinical efficacy and adverse reactions etc.were observed.Results After treatment,in the two groups,the differences were statistically significant in the platelet aggregation and plasma viscosity compared with before treatment (t =4.412,4.215,3.984,4.608,all P ＜ 0.05),and the differences were statistically significant in the improvement of the observation group compared with the control group(t =5.553,4.436,all P ＜ 0.05).The total effective rate of the observation group was 88.9％,that of the control group was 68.9％,the difference was statistically significant between the two groups (x2 =2.702,P ＜0.05).The incidence of adverse reactions in the observation group was 15.6％,which in the control group was 17.8％,the difference was not statistically significant between the two groups (x2 =0.040,P ＞ 0.05).Conclusion On the basis of conventional therapy,Danhong injection combined with edaravone in the treatment of acute ischemic stroke can significantly improve the clinical symptoms of the patients,improve the clinical efficacy and has good safety and great clinical significance.

ObjectiveTo apply the three-dimensional reconstruction technique of multi-slice spiral CT in spine disease.Methods40 cases with spine disease who accepted three-dimensional reconstruction technique of multi-slice spiral CT were reported.ResultsCalcification of posterior longitudinal ligament,narrow of disc space or neural foramen,varied fracture bone sclerosis and spondylolisthesis could be found with this technique.ConclusionThree-dimensional reconstruction technique may be recommended for the diagnosis of spine disease during recovering.

Objective To investigate the application of multi-slices spiral CT in the diagnosis of cerebrospinal fluid rhinorrhea. Methods 40 cases with cerebrospinal fluid rhinorrhea were examined with multi-slices spiral CT, and their coronal reconstruction images were analyzed. Results Single skull bone defect was found in 30 cases. The leakage positions were showed and consistent with the position of the skull defect in 28 cases. Multiple skull bone defects were found in 10 cases which showed more than 2 leaks. Leakage position was not showed in 2 cases, but the paranasal sinus cavity with high density fluid might suggest leakage location. The leakage positions were located in ethmoid sinus (28 cases), posterior wall of frontal sinus (9 cases), superior wall of sphenoid sinus (3 cases). Conclusion Multi-slices spiral CT is an effective examination to determine location of cerebrospinal fluid leak.

This paper briefly reviewed the history and current development of traditional Chinese medicine(TCM)in the United Kingdom and introduced TCM associations and TCM colleges and universities in detail in this country.This paper provided a reference for comprehensively understanding the current TCM associations.education and researching institutes in the United Kingdom.