Objective To evaluate the role of PI3K-Akt-HIF-1α signaling pathway in dexmedetomidine-induced reduction of damage to the unventilated lung during one-lung ventilation (OLV) in rats.Methods Forty adult male Sprague-Dawley rats,weighing 250-350 g,were randomly divided into 4 groups (n=10 each) using a random number table:two-lung ventilation TLV) group,OLV + normal saline group (group OLV),OLV + dexmedetomidine group (group D),and OLV + dexmedetomidine +LY294002 group (group DL).Bilateral lungs were ventilated for 2.5 h in group TLV,and the right lung was ventilated for 2 h followed by 0.5 h TLV in the other groups.The rats were sacrificed,the left lung was removed for determination of wet to dry lung weight ratio (W/D ratio),and expression of phosphorylated Akt (p-Akt) and hypoxia-inducible factor-1 alpha (HIF-1α) (by Western blot).Results Compared with group TLV,W/D ratio was significantly increased,the expression of p-Akt was down-regulated,and the expression of HIF-1α was up-regulated in the other three groups (P＜0.05).Compared with group OLV,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-lα was up-regulated in D and DL groups (P＜0.05).Compared with group DL,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-1α was up-regulated in group D (P＜0.05).Conclusion PI3K-Akt-HIF-1α signaling pathway is involved in dexmedetomidine-induced reduction of damage to the unventilated lung during OLV in rats.

The deposition of amyloid β-protein (Aβ is the key pathological basis of cerebral amyloid angiopathy (CAA) and Alzheimer's disease (AD). The current studies emphasis that the stability of internal environment between cells of the neurovascular unit is the key to maintain normal brain function. This article reviews the relationship between the Aβand the neurovascular unit injury, and we expect to provide some new ideas for the clinical prevention and treatment of CAA and AD.

Asia ; epidemiology ; Humans ; Non-alcoholic Fatty Liver Disease ; epidemiology ; Prevalence

There are many therapeutic drugs for rheumatoid arthritis(RA) treatment,but the pathogenesis of RA have not been fully understood,therefore no curable drug has been found.With increasing knowledge of the inflammatory process,especially in signaling,some drugs modifying disease progress and interfering signaling pathways have been studied.Cytokine inhibitors such as tumor necrosis factor-blocking agents and IL-1 receptor antagonist in clinical trials and in practice have resulted in dramatic improvement in RA disease control.Important molecular mechanisms of actions on total glucosides of paeony(TGP) and Glucosides of Chaenomeles speciosa(GCS) were showed to modulate G protein-AC-cAMP and Ras-MAPKs signaling pathways and to inhibit synoviacytes proliferation.This review focuses on agents which target various cytokines,other inflammatory mediators,antigen of cells,and signaling pathways.

DICOM standard is a compulsory international standard for the establishment of Integrated Hospital Information System (IHIS) and Picture Archiving and Communication System (PACS). This paper introduces such information of DICOM3.0 as its generation, components, operational mechanism and application. To understand DICOM standard exactly is helpful for the efficient operation of PACS.

Improved medical postgraduate reexamination system is essential to the quality of students. In this study, organization of medical postgraduate reexamination was discussed from the perspectives of preliminary arrangements of medical postgraduate reexamination, fairness of reexami-nation process and improvement of the follow-up after the reexamination. Sufficient attention on the re-examination, effective supervision of the reexamination process and control of human factors were the three main factors for the successful medical postgraduate reexamination and effective guarantee for the improvement of postgraduate quality.

Objective To observe the clinical effect of long-range treatment for elderly patients with reflux esophagitis.Methods 124 reflux esophagitis patients were randomly divided into observation group (62 cases) and control group (62 cases) according to random number method.The observation group received long-range treatment (maintain treatment for 3 months after the end of the routine treatment),while the control group received conventional treatment,symptoms score and endoscopic healing of two groups were compared.Results Before treatment and 3 months after treatment,two groups of reflux symptom scores were not significantly different (t =1.325,1.241,all P ＞ 0.05),6 and 12 months after treatment,reflux symptoms score of the observation group was significantly lower than that of the control group (t =24.325,18.124,P ＜ 0.01),after total treatment,the good treatment effect rate of the observation group was 90.0%,which was higher than that of the contol group(55.7%)(x2 =17.902,P ＜0.01),healing of esophagitis excellent rate of the observation group was 86.7%,which was higher than that of the contol group (57.4%) (x2 =12.844,P ＜0.01).Conclusion Long-range treatment of symptomatic improvement has good effect in elderly the reflux esophagitis,the lesion healing to improve the quality,which is worthy of further promotion research.

Mass spectrometry is an important and powerful tool for protein quantification. With the in-depth development of quantitative proteomics, limitations of classic MS based quantification methods, such as complicated matrix interference and throughput/capacity limitation, start to appear. Recent progress of series novel MS based techniques provide effective solutions for the limitations of relative and absolute proteomic quantification, including synchronous precursor selection ( SPS ) , mass defect isobaric labeling, parallel reaction monitoring ( PRM) , multiplexing acquisition ( MSX) , and various novel data independent acquisition ( DIA) modes. Here we summarized the current limitations of quantitative proteomics, reviewed the latest MS based quantification approaches, and discussed the features and advantages of these novel techniques for quantitative proteomic application.

Objective To investigate the clinical characteristics and the treatment of the infection of heli-eobacter pylori in gastric stump. Methods The clinical data of 210 patients who were examined by gastroscopy for gastric stump and 200 randomly selected patients who had no history of operation due to gastrointestinal diseases and received gastroscopy and Hp detections were retrospectively analyzed. Results The Hp infective rate in gastric stump was 26.2 %, and the Hp infective rates had no significant difference among different operative causes. The Hp positive rate after Billroth-Ⅰ operation was obviously higher than that after Billroth-Ⅱ type operation;the Hp posi-tive rate in the patients with bile regurgitation was lower than that in the patients without bile regurgitation. The Hp infective rate became lower as the time after subtotal gastrectomy prolonged. The clearance rate of anti-Hp treatment was 87.3 %. Conclusion The Hp infective rate in gastric stump is lower, but the Hp lesion in gastric stump ks ag-gravated. The anti-Hp therapeutic program which is commonly used at present plus gastric mucosa protectant and gastric motive power drugs are suitable for treating Hp infection in gastric stump, and have definite value in clinical application.

Objective To summarize the experiences of logistics support in disaster areas stricken by the catastrophic Wenchuan earthquake with the aim to improve the ability to effectively meet various military missions. Methods The experiences of logistics support in disaster areas stricken by the catastrophic Wenchuan earthquake were reviewed and analyzed retrospectively. With the author's knowledge of commanding the health service in rescue work, a proposal was put forward on the organization of military logistics and health support to effectively meet various military missions in the future. Results The disaster areas stricken by the strong Wenchuan earthquake were about 500 thousand km2, in which about 130 thousand km2 areas were heavily stricken, and about 4.3 million casualties needed to be treated. The peculiarities of the rescue support in the disaster areas were as follows: urgency in mission, difficulty in coordination, urgent and rapid changes in tasks, and difficelty in ensuring supply. For the military logistic service, the specific services in disaster areas were: to strictly carry out the commands from high authorities and determination to carry out the rescue work of health support; to unify the commands, and coordinate as a whole to enhance the efficiency of health service command; to carry out rational deployment of medical teams and rapidly organize a combined military and civilian medical support system; to dexterously utilize every single resource of health service, and continuously enhance the efficiency of health service. By means of high efficiency of health service, the fighting capacity of troops in carrying out the task had been withheld and enhanced, ensuring timely treatment of the injured, and no outbreak of epidemic, which used to prevail after a strong earthquake. Conclusions In view of existing problems in medical support in rescuing the casualties in this disaster, such as insufficient preparedness of health service in dealing with catastrophic natural disaster, imperfect organization and coordination system, and backward in equipments and techniques, and the prospects that the necessity of developing military health service to meet the needs of various military tasks in the future, it is suggested that: the capacity to respond to meet sudden accidents should be honed, capacity of coordinated action in carrying out tasks by combined forces should be enhanced; the levels of surgical expertise and medical support should be elevated, and the construction of logistics forces should be strengthened.