Objective:To explore whether the introduction of web problem based learning (WPBL) teaching method in medical humanities English class under the background of new medical science is helpful to the course learning and the improvement of students' humanistic quality.Methods:Taking 602 students from Batch 2017 clinical medicine in Xi'an Medical University as the experimental group and 610 students from Batch 2018 as the control group, the research lasting a semester was carried out around eight modules. The control group adopted the traditional teaching mode, while the experimental group used the WPBL mode for teaching relying on the Blue Pigeon Cloud Network Language Learning Platform. After teaching, through comparing the final exam results and issuing self-made questionnaires, the teaching effects of the two methods on students' self-study ability, thinking ability, teamwork ability and knowledge application ability were compared. The data were statistically processed by SPSS 20.0.Results:The final average score of the experimental group (62.13±2.11) was higher than that of the control group (60.42±2.03), with significant differences ( P < 0.05). According to the statistical analysis of the questionnaire, there were differences between the two groups in evaluating the teaching effect, and the proportion of the experimental group was over 60%, significantly higher than that of the control group. 93.3% of the students in the experimental group were satisfied with the WPBL teaching method, while only 43.0% of the students in the control group were satisfied with the traditional teaching method, showing a significant difference between them. The evaluation of students in the experimental group were higher than the control group in terms of self-study ability, data access ability, knowledge exploration ability, language expression ability and problem finding and solving ability. 91.1% of students in the experimental group believed that the introduction of WPBL teaching mode in the course would help to improve their medical humanistic quality, which was significantly higher than the proportion of students in the control group. Conclusion:The empirical study shows that the introduction of WPBL teaching method in medical humanities English course can not only enable students to master learning skills, but also strengthen students' humanistic quality, thus promoting the development of medical education.

In order to study of etiology, pathogenesis, and therapy of endometriosis(EM) through animal models, we review the literature on rodent and primate models of endometriosis, including establishment of models and their applications.We hope that animal models provide useful tools for the studies of endometriosis.

Objective:To study the influence of transfer payment on the equalization of medical and health services.Methods:Based on panel data of China's 23 provinces from 2000 to 2014.the medical and health services equalization indexes were measured and developed as the explained index.The explanatory variables such as transfer payment,per capita GDP and health care input were involved.The threshold value of time and transfer payment were applied to developed to conduct the panel threshold regression.Results:Transfer payment significantly promoted the equal distrihntion of health service resources,but the elastic declined if transfered more than 33 billion yuan as the threshold.Meanwhile,there was a threshold effect that the elastic of transfer payment to the equalization of China's medical and health service declined after NCMC in 2003.Conclusion:To further increase transfer payment,it needed to enlarge the new farmers and scope,optimize the equal distribution of health service resources.

Objective: To explore the mechanism of phytoestrogen (genistein) on uterine endometrial cancer cells and provide the theory foundation for using phytoestrogen in clinical practice. Methods: Uterine endometrial cancer cell lines (ishikawa) were cultured in vitro at different genistein concentration, and the expressions of the two estrogen subtypes of endometrial cancer cells were observed by real-time PCR. And two treatment groups, estradiol and progestin, were used as positive controls. Results:(1) Low concentration of genistein could enhance the expression level of ER? mRNA of endometrial cancer cells, which was obviously weaker than that of the estradiol group. On the other hand, low concentration of genistein could decrease the expression level of ER? mRNA of endometrial cancer cells, however estradiol had hardly any effect on this .(2) High concentration of genistein could decline the expression levels of ER? and ER? mRNA of endometrial cancer cells, whose effect was weaker than that of progestin. Conclusion: The expression levels of ER? mRNA and ER? mRNA of endometrial cancer cells vary with genistein concentrations. The experimental results suggest that genistein may act as estrogen receptor regulator.

In the light of developing trend and reality of domestic pharmaceutical supervision and management we propose the guidance of drug market, using the scientific method, making supervision as center, taking emphasis on basic units, strengthening in vertical section, popularizing in horizontal section, expanding scale etc as guiding thoughts. In order to strengthen the basic pharmaceutical supervision and management the suggestion of ten kinds of strategic changes must be realized.

Objective To study the spatial spillover effects of medical and health resources on the hospital innovation.Methods Based on panel data of 31 provinces of China from 2000 to 2014,and with the method of Spatial Durbin Model(SDM),the paper does the regression with the sample of all provinces,eastern,central and western provinces,through using provincial patent number as explained variable,and using the provincial medical institutions of beds,per capita GDP,health care spending as explanatory variables.Results Medical and health resources have a significant role in promoting the hospital innovation,not only for local province but also for adjacent provinces,namely there is spillover effect;the promotion effect of medical resources for local and neighbouring province hospital innovation is almost the same;for eastern and central provinces,there is a positive effect of medical resources to hospital innovation,and for central province,the spillover effects exist.Conclusion The medical and health resources should be further increased,a provincial communication platform of resources and innovation be built,the east and western provinces spillover effect be promoted,to drive the reform of the supply side with innovation.

Colorectal Neoplasms ; pathology ; Humans ; Neoplasm Staging

Background Oxidative stress is one of the pathogenesis mechanisms of diabetic complications.Melatonin is the most powerful antioxidant in living organism.Seldom study on the effects of melatonin on diabetic complications is found in China now.Objective This study aimed to detect the expression and alteration of malonadehyde(MDA),glutathione(GSH)and glial fibrillary acidic protein(GFAP)in retina with experimental diabetes and explore the effects of melatonin on retinal oxidative stress in diabetic rats. Methods The animal models of diabetes were established by injection of 2％ streptozocin via caudal vein(STZ,45 mS/kg)in 48 6-week-old clean SD rats.Isometric citric acid buffer solution wag injected in 24 normal.rats as control group.Seven days after injection of STZ,melatonin(10 mg/kg)was injected into the abdominal cavity of 24 diabetic rats in melatonin group dailv,and the equal volume of normal saline was injected in other 24 diabetic rats(model rats)and 24 rats in normal control group.The animals were sacrificed and retinas were obtained in three groups in 4,6 and 8 weeks respectively.Immunohistochemistry and Western blot were used to detect the expression of GFAP in rat retina.The levels of MDA and GSH in retina homogenate were assayed by spectrophotometer.This experiment complied the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results The expression of GSH in model group was lower than that in melatonin group or control group at 4,6 and 8 weeks (all P<0.05).However,no statistically significant difference was seen in the expression level of GSH between melatonin group and normal control group at 4 and 6 weeks(P>0.05),and in the eighth week,expression of GSH in melatonin group was lower than that in control group(P<0.05).The expressions of MDA and GFAP were more prominent in the model group than in melatonin group and control group in 4,6 and 8 weeks(P<0.05),but no significant difference was found between melatonin group and control group at 4 and 6 weeks(P>0.05).The expressions of MDA and GFAP in melatonin group were higher than those in control group at 8 weeks(P<0.05).Conclusion Melatonin plays a powerful effect in protecting the retina from oxidative stress in diabetes retina by downregulating the MDA level,upregulating GSH level and inhibiting GFAP expression.

AlM: To learn the changes of the tear film before and after the trabeculectomy of glaucoma and explore the incidence of dry eye and the prevention and control measures.METHODS:The 36 patients (60 eyes) of glaucoma were examined in detail before 3d of trabeculectomy and after the surgery at 3, 7, 14 and 30d. The examinations include lower eyelid central river of tears, break-up time ( BUT) , Schirmer I test ( S I t ) and staining scores of corneal fluorescein under slit lamp microscope.RESULTS:The tear meniscus height of central lower eyelid was increased and the tear film BUT was shortened at the same time, the scores of S I t was reduced and corneal fluorescein staining score was increased at postoperative 3 and 7d compared with that of preoperation. The tear meniscus height of central lower eyelid, tear film BUT and Slt and score of corneal fluorescein staining began to recover in most of the affected eyes after surgery 14d. At 30d after surgery, 22%of patients tear film failed to recover to the preoperative level; dry eye occured in 18% preoperative eyes with normal tear film.CONCLUSlON:Trabeculectomy of glaucoma may affect the stability of the tear film and some patients showeing obvious dry eye and should be intervened and treatmented timely.

Objective To evaluate urodynamic diagnosis of intrinsic sphincter deficiency (ISD) in female stress urinary incontinence. Methods Leak-point pressures (LPP) and maximum urethra close pressures (MUCP) were detected in 30 patients with female stress urinary incontinence,among whom there were 11 cases with type Ⅲ,19 type Ⅱ/Ⅲ. Results Of type Ⅲ incontinence the LPP was less than 50 cmH 2O in 10 out of 11 and in 17 out of 19 type Ⅱ/Ⅲ incontinence the LPP 50～100 cmH 2O ( P