ObjectiveTo identify the chemical composition of stem, leaf and peel ofCynanchumchinese R.Br..MethodsFourier IR spectra, the second derivative spectrum and two-dimensional correlation spectroscopy were adopted to identify IR of different parts ofCynanchumchinese R.Br..ResultsIt was found that the IR spectra of the leaf was similar to that of the peel but was different from the stem. What’s more, in the second derivative spectra of the leaf, it showed that the absorption peak was strong at the position of 1 543 cm-1, 1 515 cm-1, 1 499 cm-1 and 1 467 cm-1, respectively and there existed carbonyl absorption peak at 1 738 cm-1 and 1 659 cm-1 in addition. Therefore, it was inferred that flavonoids were the major components while less in the stem and peel.ConclusionsThe differences of the three parts aboutCynanchumchinese R.Br. were studied through IR spectrum macroscopically, which provided reference for exploring the constituents and clinical medication.

OBJECTIVE:To explore the effects of ethanol extracts of Solanum lyratum Thunb(ST) on induction of apoptosis and the expression of apoptosis associated genes fas and caspase-3 in human lung cancer SPC-A-1 cells.METHODS:Cultured human lung cancer SPC-A-1 cells were randomly divided into the control group,ethanol extracts of ST treated groups(2.5、5、10 mg?L-1)and the positive control group(cisplatin).After treatment with drug for 48 h,the proliferation inhibitory rate was evaluated by MTT assay,induction of cell apoptosis rate was determined by TUNEL method,the expression of fas and caspase-3 mRNA was detected by semi-quantitive RT-PCR.RESULTS:Compared with control group,the inhibitory rate was increased obviously(P

Intravascular stent has been widely used in clinic to treat cardiovascular diseases, but the complications gradually present. Vascular endothelialization following stent implantation is a key factor that influences the biocompatibility of material and human body. After stent implantation, vascular endothelium function is abnormal, inducing endodermis shedding, platelet aggregation, inflammatory reactions, vascular smooth muscle cell proliferation and migration, and excellular matrix formation. These pathological reactions cause in-stent restenosis. So repair and functional changes of vascular endothelial cells play an important role in in-stent restenosis. To deeply understand the vascular endothelialization following implantation of various stents, this paper discussed rapid vascular endothelialization by retrieving articles so as to search a method to solve this problem.

AlM:To observe the clinical effect of 2. 2mm micro-incision phacoemulsification ( Phaco ) combined intraocular lens( lOL) implantation in the eyes with short axial length and compared with traditional 3. 0mm incision phacoemulsification operation for clinical work and research objective information.METHODS:ln this prospective clinical control study, 60 cases (60 eyes) with cataract performed 2. 2mm micro-incision phacoemulsification combined lOL implantation ( micro-incision group, implanted Akreos Ml60 lOL ) and 3. 0mm incision phacoemulsification operation ( small incision group, implanted Akreos Adapt lOL ) , whose axial length ranged 17. 68 ~ 21. 32mm, average 20. 35 ± 0. 61mm. Average axiall ength respectively of two groups:20.57±0.39mm and 20.21±0.52mm. Effect Phaco time ( EPT ) and average ultrasonic energy ( AVE ) were recorded. The best corrected visual acuity ( BCVA ) , intraocular pressure ( lOP ) , anterior chamber depth ( ACD) , corneal endothelial cell density and the operative complications were observed postoperatively. All cases were followed up at 1, 7, 30 and 90d after operation.RESULTS:EPT and AVE between two groups had no statistical significance(P>0. 05). At the 1, 7 and 30d after surgery, induced astigmatism ( SlA ) of micro-incision group was lower than that of small-incision group. SlA between two groups had statistically significant ( P <0. 05), but in the 90d SlA between two groups had no statistical significance(P>0. 05). At the 7d, the BCVA between two groups had statistical significance(P<0. 05), but at the 30 and 90d, BCVA between two groups had no statistical significance(P>0. 05). At the 7, 30 and 90d, corneal endothelial cell density of micro-incision group was higher than that of small incision group. Corneal endothelial cell density between two groups had no statistical significance. The mean ACD was significant increased postoperatively than preoperatively and had no statistical significance ( P>0. 05 ) . The anterior chamber stabilized during the operation. There was not incision hot harmed during operation and postoperation.CONCLUSlON:Compared with 2. 2mm micro-incision and traditional 3. 0mm incision phacoemulsification, two groups have the same safety and 2. 2mm micro-incision phacoemulsification has small SlA, organization small harm and improved visual acuity in early stage.

Coblation is a new technology widely used in the otolaryngology in recent years.This article described the principle,indications,methods and advantages which the coblation was used in pharynx and throat diseases in children.It's a safe,effective and minimally invasive way.

OBJECTIVE:To observe the effects of praeruptorin A for brachial plexus block anesthesia in patients receiving up-per extremity fractures surgery. METHODS:68 patients receiving upper extremity fracture surgery were randomly divided into trial group and control group with 34 cases in each group. Trial group was given praeruptorin A 0.2 mg/kg intravenously before brachial plexus block anesthesia,and control group was given equivalent volume of 0.9% sodium chloride injection. The anesthesia induc-tion time,the pain reflex disappearance time and postoperative recovery time were compared between 2 groups,and hemodynamic parameters and RPP were recorded in 2 groups before anesthesia (T0),immediately after anesthesia (T1),5 min after anesthesia (T2),10 min after anesthesia (T3),20 min after anesthesia (T4) . RESULTS:Anesthesia induction time,pain reflex disappear-ance time and postoperative respiratory recovery time in trial group were all significantly shorter than in control group,with statisti-cal significance(P<0.05). SBP,DBP,HR and MAP of trial group were lower than those of control group at T2,T3,T4,with sta-tistical significance(P<0.05). RPP of trial group was significantly lower than that of control group at T1,T2,T3 and T4,with sta-tistical significance (P<0.05). CONCLUSIONS:For brachial plexus anesthesia of upper extremity fractures surgery,preoperative application of praeruptorin A can shorten anesthesia induction time,pain reflex disappearance time and postoperative respiratory re-covery time,and reduce the fluctuation of hemodynamics.

Aluminum is a common metal element,and its nervous toxicity probably has an effect on the learning and memory mechanism. Long-term potentiation (LTP) is an available electrophysiology-model,cAMP/protein kinase A (PKA) approach is an important signal transduction pathway of nerve cells,and plays an important role in the process of learning and memory mechanism. Aluminum has some adverse effects on learning and memory through influencing some links,such as neurotransmitter,Ca2 + concentration,protein kinase and so on. According to the recent studies in this field,in electrophysiology and biochemistry,the present paper reviewed the effects of aluminum on the learning and memory mechanism,as well as the role of cAMP/PKA approach.

BACKGROUND:Less Invasive Stabilization System (LISS) has been shown to hold the same biomechanical strength to proximal femoral nail anti-rotation.OBEJCTIVE:To investigate the safety and effectiveness of the reversed LISS plates for senile femoral intertrochanteric fractures.METHODS:Thirty elderly patients with femoral intertrochanteric fractures were treated with reversed LISS plates.The operation time,incision length and total blood loss (intraoperative plus volume of drainage 1 day postoperative) were recorded.Physiologic and Operative Severity Score for the Enumeration of Mortality and Morbidity (POSSUM) and modified POSSUM scoring systems were used to predict the incidence of complications and mortality,and then the real incidence of complication and mortality was compared to evaluate the safety of reversed LISS plates.The clinical effectiveness was evaluated by fracture healing time and Harris scores at the last follow-up.RESULTS AND CONCLUSION:(1) The incidence of complications showed significant difference between predicted (46.05％) and real (13.3％) results (P=0.05),but the mortality did not differ significantly between predicted (3.93％) and real (0％) results (P=1).(2) The fracture healing time was 21-32 weeks,and the excellent and good rate in Harris scores at the last follow-up was 83.3％.(3) These results indicate that the reversed LISS plates are easy to operate,safe and obtain good efficacy in the treatment of senile femoral intertrochanteric fractures.

Adult ; Aged ; Electrocardiography ; Humans ; Male ; Middle Aged ; Radiography, Thoracic ; Silicosis ; complications ; diagnostic imaging

Objective: To study the influence of Wnt5a gene silence on the proliferation, migration and invasion of lung squamous carcinoma cells. Methods: A recombinant plasmid pH1-siRNAWnt5a- was constructed and used to deliver small interference RNA (siRNA) targeting Wnt5a in SK-MES-1 cells; the transfected cells were screened to establish a stable transgenic cell line. MTT, cell cycle and Transwell assays were employed to evaluate the effect of Wnt5a gene silence on the proliferation, migration and invasion of lung squamous carcinoma cells. Results: Western blotting assay revealed that Wnt5a was lowly expressed in SK-MES-1Wnt5a- (13.6%). The proliferation index (PI) of transgenic cell line was slightly lower than that of the control cell line ([28.3±3.8] % vs [30.5±5.2]%). The migration and invasion capabilities of SK MES-1Wnt5a- cells were (47.3±9.2)% and (39.7±11.7)% of the control cells, respectively. Conclusion: Low Wnt5a expression can significantly inhibit the migration and invasion capabilities of SK-MES-1 cells, indicating that WntSa might be a potential target for gene therapy of lung squamous carcinoma.