Asparaginase(ASP) is an important drug in the treatment of childhood acute lymphoblastic leukemia and non-Hodgkin lymphoma.Asparaginase associated pancreatitis (AAP) is the main treatment-adverse events of asparaginase.After reviewing the recent foreign literatures about AAP and the Chinese expert about polyethylene glycol conjugated asparaginase (PEG-ASP) in the treatment of acute lymphoblastic leukemia and malignant lymphoma with asparaginase,conclude and analysis the data about childhood AAP and show the epidemiology,clinical features,early diagnosis and effective treatment of children with AAP.Make clinical compare of L-asparaginase and PEG-ASP.Based on the full grasp of the relevant data,analyzing,introducing and integrating,this may be helpful to the diagnosis and treatment of childhood AAP.

Since upper limb function disorder exists in many children with cerebral palsy, it is improtant to assess and treat the disorder of the upper extremities. The common assessments include muscle tone, range of motion and upper extremity assessment. The goal of therapy is to decrease the muscle tone and increase the range of motion. In addition we should emphasize the functional ability and purposeful skill of the upper extremity.

Antihypertensive Agents ; therapeutic use ; Humans ; Vaccines

Angiotensin-Converting Enzyme Inhibitors ; therapeutic use ; Follow-Up Studies ; Heart Failure ; drug therapy ; Humans ; Retrospective Studies

Gene therapy of spinal cord injury (SCI) is the most promising method compared with the others, because it doesn't involve the problems of resource and higher exclusion which respectively exists in fetal nerve transplantation and peripheral nerve transplantation. There are two ways of gene therapy to be chosen: one is to transfer objective genes to the target-cells in vivo directly; the other is to transfer objective genes to one proper kind of transplantable cells firstly, then graft the highest expressing cells to the target-cells in vivo. To realize the transfer of genes to cells, two measures are used in common: physical or chemical measure such as micro infection et al and biochemical measure i. e. gene modified defective virus. Although there are some questions unresolved in this field, the clinical value of gene therapy of SCI in the future is depended.

We retrospectively analyzed 23 cases of histiocytic necrotizing lymphadenitis (HNL) admitted to our hospital as fever of unknown origin (FUO).Fever,lymphadenopathy and skin rash were the most common clinical manifestation.The major laboratory features included cytopenia,abnormal liver enzyme and elevated inflammatory markers.Three of the 23 cases were finally diagnosed as infectious disease,5 as autoimmune disease,while the left 15 remained as HNL during the follow-up.Four of the 15 HNL patients resolved spontaneously,while the other 11 relieved by steroid use.Two patients relapsed during the follow-up.HNL was one of the rare causes of FUO.Since it would be accompanied with infectious,autoimmune or malignant diseases,long follow-up is necessary.

Objective To investigate the test analysis of tumor marker CA19‐9 ,CEA ,CA242 ,CA125 and AFP for jointly assess‐ing the chemotherapeutic effect of gastric cancer .Methods Totally 160 patients with gastric cancer in our hospital from January 2010 to December 2013 were selected as the research subjects .The expressions of related tumor markers were detected before treat‐ment and at 3 months after treatment .The relationship between the TNM staging and the tumor markers ,changes of tumor markers before and after treatment ,and changes of tumor markers in the patients with recurrence and metastasis ,and patients without recur‐rence and metastasis were analyzed .Results Different TNM staging had significant correlation with tumor markers associated posi‐tive expression rate .The positive expression rate of related tumor markers in the patients with stage Ⅲ and stage Ⅳ were signifi‐cantly higher than that in the patients with stage Ⅰ - Ⅱ .The positive expression rates of CA19‐9 ,CA242 ,CA125 and AFP after chemotherapy were significantly decreased compared with before treatment (P<0 .05) ,while the CEA level had no statistically sig‐nificant difference before and after treatment (P>0 .05) .After treatment ,the CEA ,CA19‐9 ,CA242 ,CA125 and AFP positive ex‐pression rates were declined ,moreover showing statistical differences compared with pre‐treatment (P<0 .05) .The CEA ,CA19‐9 , CA242 ,CA124 and AFP levels in the patients with recurrence and metastasis were significantly higher than those in the patients without recurrence and metastasis (P<0 .05) .Conclusion CA19‐9 ,CEA ,CA242 ,CA125 and AFP have a significant correlation with tumor TNM staging ,their expression level and positive expression rates have certain correlation with the prognosis in the pa‐tients with gastric cancer ,which is worthy of focus in clinic .

Immunotherapy, particularly lung cancer vaccine therapy, has advantages of less side effects and low costs over classi-cal therapies of lung cancer, such as surgery, radiotherapy, and chemotherapy, as well as the relatively new molecular targeting therapy. PhaseⅢclinical trial for many kinds of non-small cell lung cancer (NSCLC) vaccines is ongoing based on deeper understanding of im-mune system and the expression of tumor antigens. This review summarizes the development of NSCLC vaccines.

Objective To explore effect of miR‐302a on proliferation and apoptosis in folate deficiency mouse embryonic stem cell (mESC) .Methods The cases were divided into complete culture medium group (control group) ,folate‐deficient culture medi‐um group (folate‐deficient group) ,folate‐deficient culture medium plus miR‐302a mimic group (miR‐302a group) .The expression of miR‐302a was examed by RT‐PCR in control group and folate‐deficient group .To construct miR‐302a mimic and then was trans‐fected into the folate‐deficient culture medium mESC .Effect of miR‐302a mimic on mESC viability was detected by MTT assay ,the effect of miR‐302a mimic on mESC apoptosis was examed by Annexin V‐FITC/PI flow dual‐staining method ,the effect of miR‐302a mimic on mESC cycle was examed by flow cytometry .The activation of phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) and expression of CyclinD1 ,p21 and p27 was assayed by Western blot . Results The expression of miR‐302a was lower in folate‐deficient group(P<0 .01) .Compared with control group ,the viability of mESC was lower ,the apoptosis of mESC was higher ,the cell cycle was arrested in G1 phase ,the level of phosphorylation of AKT and mTOR was lower ,the expression of CyclinD1 was lower ,the expression of p21 and p27 was higher in folate‐deficient group , with statistical significance (P<0 .01) .Compared with folate‐deficient group ,the viability of mESC was higher ,the apoptosis of mESC was lower ,G1 phase was shortened ,the level of phosphorylation of AKT and mTOR was higher ,the expression of CyclinD1 was higher ,the expression of p21 and p27 was lower in miR‐302a group with statistical significance (P<0 .01) .Conclusion These results suggested miR‐302a exerted anti‐apoptosis and promote cell proliferation in folate‐deficient culture medium ,which might be related to PI3K/AKT/mTOR signal pathway .